Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G55325 AT1G55325 AT1G55325 AT1G55325 |
| fragaria_vesca | FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 FvH4_3g45550 |
| malus_domestica | MD03G1004100.v1.1 |
| prunus_persica | Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 Prupe.6G003600_v2.0.a1 |
| pyrus_communis | pycom03g00420 |
| rosa_chinensis | RchiOBHm_Chr5g0083741 |
| rosa_laevigata | RLG00000037049 |
| rosa_multiflora | Rmu_sc0001287.1_g000006 |
| rosa_roxburghii | Rroxscaffold_1G00000840 |
| rosa_rugosa | Rorug05G0485500 Rorug05G0485600 Rorug05G0485700 |
| rosa_samantha | Rh5AG538600 Rh5BG564000 Rh5CG585900 Rh5DG572400 |
| rosa_wichuraiana | Rw5G050090 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 5341 |
| Acc36I | ACCTGC | 4 cut(s) | 2873, 3294, 4783, 5341 |
| AccB1I | GGYRCC | 3 cut(s) | 1541, 1774, 4322 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1670 |
| AccBSI | CCGCTC | 1 cut(s) | 1661 |
| AccI | GTMKAC | 5 cut(s) | 1297, 1683, 3776, 4195, 4726 |
| AccII | CGCG | 1 cut(s) | 3301 |
| AciI | CCGC | 7 cut(s) | 1659, 3073, 3301, 3486, 4439, 4942, 5761 |
| AcoI | YGGCCR | 1 cut(s) | 4340 |
| AcsI | RAATTY | 6 cut(s) | 506, 622, 1871, 2098, 2332, 5666 |
| AcuI | CTGAAG | 9 cut(s) | 546, 626, 868, 1836, 2528, 2708, 3746, 5153, 5375 |
| AcvI | CACGTG | 1 cut(s) | 2234 |
| AcyI | GRCGYC | 1 cut(s) | 2393 |
| AdeI | CACNNNGTG | 2 cut(s) | 470, 1166 |
| AflII | CTTAAG | 2 cut(s) | 3368, 4103 |
| AflIII | ACRYGT | 4 cut(s) | 2031, 2231, 4413, 5402 |
| AgeI | ACCGGT | 1 cut(s) | 2084 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 5739 |
| AhlI | ACTAGT | 1 cut(s) | 4073 |
| AjiI | CACGTC | 1 cut(s) | 772 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 813, 845, 935, 967, 5081, 5113 |
| AleI | CACNNNNGTG | 2 cut(s) | 1323, 5309 |
| Alw21I | GWGCWC | 6 cut(s) | 480, 577, 2614, 5137, 5260, 5835 |
| Alw44I | GTGCAC | 1 cut(s) | 5133 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 385, 1631, 1670, 1838, 4789 |
| Ama87I | CYCGRG | 3 cut(s) | 1225, 2616, 3839 |
| ApaI | GGGCCC | 1 cut(s) | 1074 |
| ApaLI | GTGCAC | 1 cut(s) | 5133 |
| ApoI | RAATTY | 6 cut(s) | 506, 622, 1871, 2098, 2332, 5666 |
| AseI | ATTAAT | 2 cut(s) | 2750, 4896 |
| AsiGI | ACCGGT | 1 cut(s) | 2084 |
| AspLEI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4647 |
| AsuC2I | CCSGG | 3 cut(s) | 227, 4236, 4273 |
| AsuII | TTCGAA | 1 cut(s) | 1749 |
| AvaI | CYCGRG | 3 cut(s) | 1225, 2616, 3839 |
| AvaII | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| AxyI | CCTNAGG | 1 cut(s) | 482 |
| BaeGI | GKGCMC | 3 cut(s) | 1074, 3833, 5137 |
| BalI | TGGCCA | 1 cut(s) | 4342 |
| BamHI | GGATCC | 2 cut(s) | 202, 3890 |
| BanI | GGYRCC | 3 cut(s) | 1541, 1774, 4322 |
| BanII | GRGCYC | 8 cut(s) | 1074, 1623, 2614, 3185, 3671, 5260, 5282, 5835 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 2188, 2220, 2887, 2919, 4969, 5001 |
| BauI | CACGAG | 2 cut(s) | 65, 4149 |
| BbrPI | CACGTG | 1 cut(s) | 2234 |
| BbsI | GAAGAC | 4 cut(s) | 2087, 2418, 3892, 4215 |
| Bbv12I | GWGCWC | 6 cut(s) | 480, 577, 2614, 5137, 5260, 5835 |
| BbvCI | CCTCAGC | 1 cut(s) | 996 |
| BceAI | ACGGC | 2 cut(s) | 2426, 3024 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1535, 1569, 3830, 3864 |
| BciVI | GTATCC | 3 cut(s) | 1420, 2400, 4552 |
| BclI | TGATCA | 4 cut(s) | 78, 937, 1876, 5572 |
| BcnI | CCSGG | 3 cut(s) | 227, 4236, 4273 |
| BcuI | ACTAGT | 1 cut(s) | 4073 |
| BfoI | RGCGCY | 3 cut(s) | 1363, 2602, 4648 |
| BfrI | CTTAAG | 2 cut(s) | 3368, 4103 |
| BfuAI | ACCTGC | 4 cut(s) | 2873, 3294, 4783, 5341 |
| BfuI | GTATCC | 3 cut(s) | 1420, 2400, 4552 |
| BglII | AGATCT | 1 cut(s) | 3923 |
| BmcAI | AGTACT | 3 cut(s) | 603, 3151, 4005 |
| Bme18I | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 5739 |
| BmeT110I | CYCGRG | 3 cut(s) | 1225, 2616, 3839 |
| BmgBI | CACGTC | 1 cut(s) | 772 |
| BmrI | ACTGGG | 1 cut(s) | 5642 |
| BmuI | ACTGGG | 1 cut(s) | 5642 |
| BpiI | GAAGAC | 4 cut(s) | 2087, 2418, 3892, 4215 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1021, 1053, 3566, 3598 |
| BpmI | CTGGAG | 5 cut(s) | 348, 1647, 4766, 5274, 5774 |
| Bpu10I | CCTNAGC | 1 cut(s) | 996 |
| Bpu14I | TTCGAA | 1 cut(s) | 1749 |
| BpuEI | CTTGAG | 9 cut(s) | 276, 762, 1377, 2617, 3434, 3612, 4560, 5140, 5244 |
| BpuMI | CCSGG | 3 cut(s) | 227, 4236, 4273 |
| Bsa29I | ATCGAT | 1 cut(s) | 3468 |
| BsaAI | YACGTR | 2 cut(s) | 2034, 2234 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2571, 4951 |
| BsaHI | GRCGYC | 1 cut(s) | 2393 |
| BsaI | GGTCTC | 1 cut(s) | 2626 |
| BsaWI | WCCGGW | 2 cut(s) | 2084, 5285 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1897, 1927 |
| Bse118I | RCCGGY | 1 cut(s) | 2084 |
| Bse21I | CCTNAGG | 1 cut(s) | 482 |
| Bse3DI | GCAATG | 4 cut(s) | 2922, 3329, 3639, 3658 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2571, 4951 |
| BseCI | ATCGAT | 1 cut(s) | 3468 |
| BseJI | GATNNNNATC | 2 cut(s) | 2571, 4951 |
| BseMI | GCAATG | 4 cut(s) | 2922, 3329, 3639, 3658 |
| BseRI | GAGGAG | 3 cut(s) | 645, 2604, 5818 |
| BseSI | GKGCMC | 3 cut(s) | 1074, 3833, 5137 |
| BseYI | CCCAGC | 2 cut(s) | 1012, 1694 |
| BsgI | GTGCAG | 2 cut(s) | 1347, 3374 |
| Bsh1236I | CGCG | 1 cut(s) | 3301 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1324 |
| BshNI | GGYRCC | 3 cut(s) | 1541, 1774, 4322 |
| BshTI | ACCGGT | 1 cut(s) | 2084 |
| BshVI | ATCGAT | 1 cut(s) | 3468 |
| BsiEI | CGRYCG | 1 cut(s) | 1324 |
| BsiHKAI | GWGCWC | 6 cut(s) | 480, 577, 2614, 5137, 5260, 5835 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1225, 2616, 3839 |
| BsiSI | CCGG | 6 cut(s) | 227, 1772, 2085, 4235, 4272, 5286 |
| BslFI | GGGAC | 6 cut(s) | 362, 995, 2329, 3722, 4844, 5115 |
| BsmBI | CGTCTC | 1 cut(s) | 778 |
| BsmFI | GGGAC | 6 cut(s) | 362, 995, 2329, 3722, 4844, 5115 |
| BsmI | GAATGC | 3 cut(s) | 610, 2381, 4276 |
| Bso31I | GGTCTC | 1 cut(s) | 2626 |
| BsoBI | CYCGRG | 3 cut(s) | 1225, 2616, 3839 |
| Bsp119I | TTCGAA | 1 cut(s) | 1749 |
| Bsp120I | GGGCCC | 1 cut(s) | 1070 |
| Bsp1407I | TGTACA | 1 cut(s) | 2755 |
| Bsp19I | CCATGG | 2 cut(s) | 1779, 3810 |
| BspACI | CCGC | 7 cut(s) | 1659, 3073, 3301, 3486, 4439, 4942, 5761 |
| BspDI | ATCGAT | 1 cut(s) | 3468 |
| BspFNI | CGCG | 1 cut(s) | 3301 |
| BspHI | TCATGA | 1 cut(s) | 5610 |
| BspMAI | CTGCAG | 9 cut(s) | 1059, 1636, 2994, 3291, 3493, 4041, 4354, 4773, 4794 |
| BspMI | ACCTGC | 4 cut(s) | 2873, 3294, 4783, 5341 |
| BspQI | GCTCTTC | 3 cut(s) | 468, 1855, 4807 |
| BspT104I | TTCGAA | 1 cut(s) | 1749 |
| BspT107I | GGYRCC | 3 cut(s) | 1541, 1774, 4322 |
| BspTI | CTTAAG | 2 cut(s) | 3368, 4103 |
| BspTNI | GGTCTC | 1 cut(s) | 2626 |
| BsrBI | CCGCTC | 1 cut(s) | 1661 |
| BsrDI | GCAATG | 4 cut(s) | 2922, 3329, 3639, 3658 |
| BsrFI | RCCGGY | 1 cut(s) | 2084 |
| BsrGI | TGTACA | 1 cut(s) | 2755 |
| BssAI | RCCGGY | 1 cut(s) | 2084 |
| BssNI | GRCGYC | 1 cut(s) | 2393 |
| BssSI | CACGAG | 2 cut(s) | 65, 4149 |
| BssT1I | CCWWGG | 3 cut(s) | 197, 1779, 3810 |
| Bst2BI | CACGAG | 2 cut(s) | 65, 4149 |
| BstACI | GRCGYC | 1 cut(s) | 2393 |
| BstAFI | CTTAAG | 2 cut(s) | 3368, 4103 |
| BstAUI | TGTACA | 1 cut(s) | 2755 |
| BstBAI | YACGTR | 2 cut(s) | 2034, 2234 |
| BstBI | TTCGAA | 1 cut(s) | 1749 |
| BstDSI | CCRYGG | 2 cut(s) | 1779, 3810 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| BstFNI | CGCG | 1 cut(s) | 3301 |
| BstH2I | RGCGCY | 3 cut(s) | 1363, 2602, 4648 |
| BstHHI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4647 |
| BstMCI | CGRYCG | 1 cut(s) | 1324 |
| BstNSI | RCATGY | 9 cut(s) | 220, 548, 569, 1556, 2112, 3531, 4417, 5368, 5406 |
| BstSLI | GKGCMC | 3 cut(s) | 1074, 3833, 5137 |
| BstUI | CGCG | 1 cut(s) | 3301 |
| BstV2I | GAAGAC | 4 cut(s) | 2087, 2418, 3892, 4215 |
| BstX2I | RGATCY | 7 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 124, 4378 |
| BstYI | RGATCY | 7 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154 |
| Bsu15I | ATCGAT | 1 cut(s) | 3468 |
| Bsu36I | CCTNAGG | 1 cut(s) | 482 |
| BsuI | GTATCC | 3 cut(s) | 1420, 2400, 4552 |
| BsuTUI | ATCGAT | 1 cut(s) | 3468 |
| BtgI | CCRYGG | 2 cut(s) | 1779, 3810 |
| BtgZI | GCGATG | 1 cut(s) | 1689 |
| BtrI | CACGTC | 1 cut(s) | 772 |
| BtsI | GCAGTG | 6 cut(s) | 106, 1008, 2179, 2846, 3978, 5409 |
| BveI | ACCTGC | 4 cut(s) | 2873, 3294, 4783, 5341 |
| CaiI | CAGNNNCTG | 5 cut(s) | 385, 1631, 1670, 1838, 4789 |
| CciI | TCATGA | 1 cut(s) | 5610 |
| CfoI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4647 |
| Cfr10I | RCCGGY | 1 cut(s) | 2084 |
| ClaI | ATCGAT | 1 cut(s) | 3468 |
| CseI | GACGC | 3 cut(s) | 1286, 2382, 4313 |
| CsiI | ACCWGGT | 1 cut(s) | 4444 |
| CspAI | ACCGGT | 1 cut(s) | 2084 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2822, 2857 |
| DraI | TTTAAA | 1 cut(s) | 34 |
| DraIII | CACNNNGTG | 2 cut(s) | 470, 1166 |
| DriI | GACNNNNNGTC | 1 cut(s) | 5739 |
| EaeI | YGGCCR | 1 cut(s) | 4340 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 5739 |
| EciI | GGCGGA | 1 cut(s) | 5776 |
| Ecl136II | GAGCTC | 3 cut(s) | 2612, 5258, 5833 |
| Eco130I | CCWWGG | 3 cut(s) | 197, 1779, 3810 |
| Eco24I | GRGCYC | 8 cut(s) | 1074, 1623, 2614, 3185, 3671, 5260, 5282, 5835 |
| Eco31I | GGTCTC | 1 cut(s) | 2626 |
| Eco32I | GATATC | 1 cut(s) | 339 |
| Eco47I | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| Eco53kI | GAGCTC | 3 cut(s) | 2612, 5258, 5833 |
| Eco57I | CTGAAG | 9 cut(s) | 546, 626, 868, 1836, 2528, 2708, 3746, 5153, 5375 |
| Eco72I | CACGTG | 1 cut(s) | 2234 |
| Eco81I | CCTNAGG | 1 cut(s) | 482 |
| Eco88I | CYCGRG | 3 cut(s) | 1225, 2616, 3839 |
| EcoICRI | GAGCTC | 3 cut(s) | 2612, 5258, 5833 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| EcoO109I | RGGNCCY | 4 cut(s) | 858, 1070, 1071, 1835 |
| EcoRI | GAATTC | 1 cut(s) | 2098 |
| EcoRV | GATATC | 1 cut(s) | 339 |
| EcoT14I | CCWWGG | 3 cut(s) | 197, 1779, 3810 |
| EcoT22I | ATGCAT | 2 cut(s) | 3658, 4661 |
| EcoT38I | GRGCYC | 8 cut(s) | 1074, 1623, 2614, 3185, 3671, 5260, 5282, 5835 |
| ErhI | CCWWGG | 3 cut(s) | 197, 1779, 3810 |
| Esp3I | CGTCTC | 1 cut(s) | 778 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 813, 845, 1402, 1434, 2097, 2129, 2996, 3028 |
| FaqI | GGGAC | 6 cut(s) | 362, 995, 2329, 3722, 4844, 5115 |
| FauNDI | CATATG | 1 cut(s) | 3421 |
| FbaI | TGATCA | 4 cut(s) | 78, 937, 1876, 5572 |
| FblI | GTMKAC | 5 cut(s) | 1297, 1683, 3776, 4195, 4726 |
| FriOI | GRGCYC | 8 cut(s) | 1074, 1623, 2614, 3185, 3671, 5260, 5282, 5835 |
| GlaI | GCGC | 4 cut(s) | 1361, 2583, 2600, 4646 |
| GsaI | CCCAGC | 2 cut(s) | 1016, 1698 |
| GsuI | CTGGAG | 5 cut(s) | 348, 1647, 4766, 5274, 5774 |
| HaeII | RGCGCY | 3 cut(s) | 1363, 2602, 4648 |
| HapII | CCGG | 6 cut(s) | 227, 1772, 2085, 4235, 4272, 5286 |
| HgaI | GACGC | 3 cut(s) | 1286, 2382, 4313 |
| HhaI | GCGC | 4 cut(s) | 1362, 2584, 2601, 4647 |
| Hin1I | GRCGYC | 1 cut(s) | 2393 |
| Hin6I | GCGC | 4 cut(s) | 1360, 2582, 2599, 4645 |
| HinP1I | GCGC | 4 cut(s) | 1360, 2582, 2599, 4645 |
| HincII | GTYRAC | 4 cut(s) | 1462, 1684, 2940, 3580 |
| HindII | GTYRAC | 4 cut(s) | 1462, 1684, 2940, 3580 |
| HindIII | AAGCTT | 1 cut(s) | 3750 |
| HpaI | GTTAAC | 1 cut(s) | 2940 |
| HpaII | CCGG | 6 cut(s) | 227, 1772, 2085, 4235, 4272, 5286 |
| Hpy99I | CGWCG | 1 cut(s) | 4143 |
| HpyCH4IV | ACGT | 5 cut(s) | 771, 1165, 2033, 2233, 3929 |
| HpySE526I | ACGT | 5 cut(s) | 771, 1165, 2033, 2233, 3929 |
| Hsp92I | GRCGYC | 1 cut(s) | 2393 |
| HspAI | GCGC | 4 cut(s) | 1360, 2582, 2599, 4645 |
| Ksp22I | TGATCA | 4 cut(s) | 78, 937, 1876, 5572 |
| KspAI | GTTAAC | 1 cut(s) | 2940 |
| LguI | GCTCTTC | 3 cut(s) | 468, 1855, 4807 |
| MabI | ACCWGGT | 1 cut(s) | 4444 |
| MaeII | ACGT | 5 cut(s) | 771, 1165, 2033, 2233, 3929 |
| MbiI | CCGCTC | 1 cut(s) | 1661 |
| MfeI | CAATTG | 4 cut(s) | 501, 4937, 5199, 5205 |
| MflI | RGATCY | 7 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154 |
| MlsI | TGGCCA | 1 cut(s) | 4342 |
| MluNI | TGGCCA | 1 cut(s) | 4342 |
| MmeI | TCCRAC | 5 cut(s) | 51, 1792, 3015, 3178, 5405 |
| Mox20I | TGGCCA | 1 cut(s) | 4342 |
| Mph1103I | ATGCAT | 2 cut(s) | 3658, 4661 |
| MscI | TGGCCA | 1 cut(s) | 4342 |
| MslI | CAYNNNNRTG | 6 cut(s) | 122, 1323, 2165, 2577, 4217, 5309 |
| Msp20I | TGGCCA | 1 cut(s) | 4342 |
| MspA1I | CMGCKG | 5 cut(s) | 995, 1694, 3488, 4355, 5395 |
| MspCI | CTTAAG | 2 cut(s) | 3368, 4103 |
| MspI | CCGG | 6 cut(s) | 227, 1772, 2085, 4235, 4272, 5286 |
| MunI | CAATTG | 4 cut(s) | 501, 4937, 5199, 5205 |
| Mva1269I | GAATGC | 3 cut(s) | 610, 2381, 4276 |
| MvnI | CGCG | 1 cut(s) | 3301 |
| NciI | CCSGG | 3 cut(s) | 227, 4236, 4273 |
| NcoI | CCATGG | 2 cut(s) | 1779, 3810 |
| NdeI | CATATG | 1 cut(s) | 3421 |
| NmeAIII | GCCGAG | 3 cut(s) | 1448, 1773, 1918 |
| NmuCI | GTSAC | 8 cut(s) | 851, 1532, 2909, 3052, 4045, 5037, 5194, 5355 |
| NsiI | ATGCAT | 2 cut(s) | 3658, 4661 |
| NspI | RCATGY | 9 cut(s) | 220, 548, 569, 1556, 2112, 3531, 4417, 5368, 5406 |
| NspV | TTCGAA | 1 cut(s) | 1749 |
| OliI | CACNNNNGTG | 2 cut(s) | 1323, 5309 |
| PaeI | GCATGC | 3 cut(s) | 220, 548, 5368 |
| PaeR7I | CTCGAG | 1 cut(s) | 1225 |
| PagI | TCATGA | 1 cut(s) | 5610 |
| PaqCI | CACCTGC | 1 cut(s) | 5341 |
| PasI | CCCWGGG | 1 cut(s) | 4827 |
| PciI | ACATGT | 2 cut(s) | 4413, 5402 |
| PciSI | GCTCTTC | 3 cut(s) | 468, 1855, 4807 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1162, 4147 |
| PctI | GAATGC | 3 cut(s) | 610, 2381, 4276 |
| PflFI | GACNNNGTC | 1 cut(s) | 1381 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1670 |
| PfoI | TCCNGGA | 1 cut(s) | 5251 |
| PinAI | ACCGGT | 1 cut(s) | 2084 |
| Ple19I | CGATCG | 1 cut(s) | 1324 |
| PmaCI | CACGTG | 1 cut(s) | 2234 |
| PmlI | CACGTG | 1 cut(s) | 2234 |
| Ppu21I | YACGTR | 2 cut(s) | 2034, 2234 |
| PpuMI | RGGWCCY | 1 cut(s) | 1835 |
| PscI | ACATGT | 2 cut(s) | 4413, 5402 |
| PshBI | ATTAAT | 2 cut(s) | 2750, 4896 |
| Psp124BI | GAGCTC | 3 cut(s) | 2614, 5260, 5835 |
| Psp5II | RGGWCCY | 1 cut(s) | 1835 |
| PspCI | CACGTG | 1 cut(s) | 2234 |
| PspFI | CCCAGC | 2 cut(s) | 1012, 1694 |
| PspOMI | GGGCCC | 1 cut(s) | 1070 |
| PspPPI | RGGWCCY | 1 cut(s) | 1835 |
| PspXI | VCTCGAGB | 1 cut(s) | 1225 |
| PstI | CTGCAG | 9 cut(s) | 1059, 1636, 2994, 3291, 3493, 4041, 4354, 4773, 4794 |
| PstNI | CAGNNNCTG | 5 cut(s) | 385, 1631, 1670, 1838, 4789 |
| PsuI | RGATCY | 7 cut(s) | 202, 947, 1120, 1502, 3890, 3923, 4154 |
| PsyI | GACNNNGTC | 1 cut(s) | 1381 |
| PvuI | CGATCG | 1 cut(s) | 1324 |
| PvuII | CAGCTG | 4 cut(s) | 995, 1694, 4355, 5395 |
| RseI | CAYNNNNRTG | 6 cut(s) | 122, 1323, 2165, 2577, 4217, 5309 |
| SacI | GAGCTC | 3 cut(s) | 2614, 5260, 5835 |
| SalI | GTCGAC | 1 cut(s) | 1682 |
| SapI | GCTCTTC | 3 cut(s) | 468, 1855, 4807 |
| ScaI | AGTACT | 3 cut(s) | 603, 3151, 4005 |
| SexAI | ACCWGGT | 1 cut(s) | 4444 |
| Sfr274I | CTCGAG | 1 cut(s) | 1225 |
| SfuI | TTCGAA | 1 cut(s) | 1749 |
| SinI | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| SlaI | CTCGAG | 1 cut(s) | 1225 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 122, 1323, 2165, 2577, 4217, 5309 |
| SpeI | ACTAGT | 1 cut(s) | 4073 |
| SphI | GCATGC | 3 cut(s) | 220, 548, 5368 |
| SsiI | CCGC | 7 cut(s) | 1659, 3073, 3301, 3486, 4439, 4942, 5761 |
| SspI | AATATT | 1 cut(s) | 537 |
| SstI | GAGCTC | 3 cut(s) | 2614, 5260, 5835 |
| StyI | CCWWGG | 3 cut(s) | 197, 1779, 3810 |
| TaiI | ACGT | 5 cut(s) | 774, 1168, 2036, 2236, 3932 |
| TaqI | TCGA | 7 cut(s) | 1226, 1366, 1683, 1749, 2624, 3468, 4141 |
| TaqII | GACCGA | 1 cut(s) | 3325 |
| TatI | WGTACW | 5 cut(s) | 601, 2224, 2755, 3149, 4003 |
| TauI | GCSGC | 4 cut(s) | 3075, 3304, 3488, 4945 |
| TseFI | GTSAC | 8 cut(s) | 851, 1532, 2909, 3052, 4045, 5037, 5194, 5355 |
| Tsp45I | GTSAC | 8 cut(s) | 851, 1532, 2909, 3052, 4045, 5037, 5194, 5355 |
| TspGWI | ACGGA | 2 cut(s) | 2208, 3883 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1381 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1670 |
| Vha464I | CTTAAG | 2 cut(s) | 3368, 4103 |
| VneI | GTGCAC | 1 cut(s) | 5133 |
| VpaK11BI | GGWCC | 5 cut(s) | 6, 177, 1835, 2810, 2875 |
| VspI | ATTAAT | 2 cut(s) | 2750, 4896 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 377, 924 |
| XapI | RAATTY | 6 cut(s) | 506, 622, 1871, 2098, 2332, 5666 |
| XbaI | TCTAGA | 1 cut(s) | 1991 |
| XceI | RCATGY | 9 cut(s) | 220, 548, 569, 1556, 2112, 3531, 4417, 5368, 5406 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 589, 2171, 2885 |
| XhoI | CTCGAG | 1 cut(s) | 1225 |
| XmiI | GTMKAC | 5 cut(s) | 1297, 1683, 3776, 4195, 4726 |
| ZrmI | AGTACT | 3 cut(s) | 603, 3151, 4005 |
| Zsp2I | ATGCAT | 2 cut(s) | 3658, 4661 |