RchiOBHm_Chr4g0415241

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015709
Physical Location & Seq
Reverse (-)
39845684 .. 39846175
492 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGAACTTGATGGAGACGCAGAAGAAGGTGAAGAAGCCACGAGCTTTACCATTTGATGGTCCACCACCAGAGTTGCCTATGGATTTAAGAAACAAGATCGAATCCCTGCATGGCTCCGAGCCCAAACTAGTAATACAGAAGGAGATTACAAATACTGATGTGAACCGTCATCATGATCGTCTGAATATGCCGAGGAACCAAGTACTGACCATGGACGTTCTCAATGAACAGGAGCGAAAAAAGGTTTTTGAACATGGTTTGGAAGTGAGAGTGATACAACCATGTCTAGAAGAGAGAAACTTACAGTTAAGGAAGTGGAATTCGCACAACGGCGGCTATTCCTATGTCTTAAACAGTGGTTGGAAAGATGTTGTTAGTAAGTGTGAGGATCGTATTAGGCCTGGTAATACAATTCAGGTTTGGTCATTCCGAGTTGATGATCGCGGAGAGGTCTGTTTTGCCCTTGTGTGCCATGGGCCAACAAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

163

Amino Acids

18.98

Weight (kDa)

8.93

Isoelectric Point (pI)

35.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 28 - 127 1.9e-20 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 56
AccII CGCG 1 cut(s) 444
AciI CCGC 2 cut(s) 333, 444
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 1 cut(s) 319
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 1 cut(s) 251
AhlI ACTAGT 1 cut(s) 127
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 1 cut(s) 396
AoxI GGCC 2 cut(s) 398, 476
ApoI RAATTY 1 cut(s) 319
AspS9I GGNCC 2 cut(s) 59, 476
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 123
BauI CACGAG 1 cut(s) 39
BccI CCATC 2 cut(s) 4, 50
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 8
BcuI ACTAGT 1 cut(s) 127
BfaI CTAG 2 cut(s) 128, 287
BisI GCNGC 1 cut(s) 334
BlsI GCNGC 1 cut(s) 335
BmcAI AGTACT 1 cut(s) 204
Bme1390I CCNGG 1 cut(s) 402
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 59, 476
BmiI GGNNCC 2 cut(s) 115, 197
BmrFI CCNGG 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 191, 210, 472
Bsc4I CCNNNNNNNGG 1 cut(s) 56
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 3 cut(s) 191, 210, 472
BseLI CCNNNNNNNGG 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 2 cut(s) 400, 478
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
BsnI GGCC 2 cut(s) 400, 478
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 4 cut(s) 96, 175, 388, 439
Bsp19I CCATGG 2 cut(s) 210, 472
BspACI CCGC 2 cut(s) 333, 444
BspANI GGCC 2 cut(s) 400, 478
BspFNI CGCG 1 cut(s) 444
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 115, 197
BspPI GGATC 1 cut(s) 396
BssECI CCNNGG 3 cut(s) 191, 210, 472
BssMI GATC 4 cut(s) 96, 175, 388, 439
BssSI CACGAG 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 210, 472
Bst2BI CACGAG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 167, 306, 356, 489
Bst6I CTCTTC 1 cut(s) 285
BstDSI CCRYGG 2 cut(s) 210, 472
BstFNI CGCG 1 cut(s) 444
BstKTI GATC 4 cut(s) 99, 178, 391, 442
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 4 cut(s) 96, 175, 388, 439
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 400
BstUI CGCG 1 cut(s) 444
BsuRI GGCC 2 cut(s) 400, 478
BtgI CCRYGG 2 cut(s) 210, 472
BtsIMutI CAGTG 1 cut(s) 361
CciI TCATGA 1 cut(s) 172
Cfr13I GGNCC 2 cut(s) 59, 476
CseI GACGC 1 cut(s) 25
Csp6I GTAC 1 cut(s) 203
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 6 cut(s) 110, 173, 211, 254, 282, 473
CviJI RGCY 7 cut(s) 37, 44, 114, 121, 336, 400, 478
CviKI_1 RGCY 7 cut(s) 37, 44, 114, 121, 336, 400, 478
CviQI GTAC 1 cut(s) 203
DpnI GATC 4 cut(s) 98, 177, 390, 441
DpnII GATC 4 cut(s) 96, 175, 388, 439
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco130I CCWWGG 2 cut(s) 210, 472
Eco147I AGGCCT 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 2 cut(s) 210, 472
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 2 cut(s) 210, 472
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 6 cut(s) 113, 176, 214, 257, 285, 476
FaiI YATR 9 cut(s) 80, 111, 174, 188, 212, 255, 283, 345, 474
FatI CATG 6 cut(s) 109, 172, 210, 253, 281, 472
Fnu4HI GCNGC 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 334
FspBI CTAG 2 cut(s) 128, 287
GluI GCNGC 1 cut(s) 334
HaeIII GGCC 2 cut(s) 400, 478
HgaI GACGC 1 cut(s) 25
Hin1II CATG 6 cut(s) 113, 176, 214, 257, 285, 476
HinfI GANTC 1 cut(s) 101
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 2 cut(s) 62, 163
Hpy188I TCNGA 3 cut(s) 118, 183, 431
Hpy188III TCNNGA 2 cut(s) 173, 287
Hpy8I GTNNAC 2 cut(s) 62, 163
HpyAV CCTTC 2 cut(s) 19, 133
HpyCH4III ACNGT 4 cut(s) 167, 306, 356, 489
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 109
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 6 cut(s) 113, 176, 214, 257, 285, 476
Kzo9I GATC 4 cut(s) 96, 175, 388, 439
LmnI GCTCC 2 cut(s) 119, 232
LpnPI CCDG 6 cut(s) 81, 119, 215, 387, 401, 414
MaeI CTAG 2 cut(s) 128, 287
MaeII ACGT 1 cut(s) 216
MalI GATC 4 cut(s) 98, 177, 390, 441
MboI GATC 4 cut(s) 96, 175, 388, 439
MboII GAAGA 3 cut(s) 34, 43, 302
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 2 cut(s) 319, 411
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 3 cut(s) 186, 379, 442
MseI TTAA 3 cut(s) 86, 308, 350
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MvnI CGCG 1 cut(s) 444
NcoI CCATGG 2 cut(s) 210, 472
NdeII GATC 4 cut(s) 96, 175, 388, 439
NlaIII CATG 6 cut(s) 113, 176, 214, 257, 285, 476
NlaIV GGNNCC 2 cut(s) 115, 197
NmeAIII GCCGAG 1 cut(s) 216
PagI TCATGA 1 cut(s) 172
PceI AGGCCT 1 cut(s) 400
PfeI GAWTC 1 cut(s) 101
PflMI CCANNNNNTGG 1 cut(s) 56
PkrI GCNGC 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 115, 197
PspPI GGNCC 2 cut(s) 59, 476
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
SaqAI TTAA 3 cut(s) 86, 308, 350
SatI GCNGC 1 cut(s) 334
Sau3AI GATC 4 cut(s) 96, 175, 388, 439
Sau96I GGNCC 2 cut(s) 59, 476
ScaI AGTACT 1 cut(s) 204
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 1 cut(s) 123
SetI ASST 6 cut(s) 30, 46, 219, 246, 420, 453
SinI GGWCC 1 cut(s) 59
SpeI ACTAGT 1 cut(s) 127
Sse9I AATT 2 cut(s) 319, 411
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 2 cut(s) 333, 444
SspMI CTAG 2 cut(s) 128, 287
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 2 cut(s) 210, 472
TaaI ACNGT 4 cut(s) 167, 306, 356, 489
TaiI ACGT 1 cut(s) 219
TaqI TCGA 1 cut(s) 99
TasI AATT 2 cut(s) 319, 411
TatI WGTACW 1 cut(s) 202
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 3 cut(s) 86, 308, 350
Tru9I TTAA 3 cut(s) 86, 308, 350
TscAI CASTG 1 cut(s) 361
TspDTI ATGAA 2 cut(s) 17, 240
TspRI CASTG 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 1 cut(s) 319
XbaI TCTAGA 1 cut(s) 286
XspI CTAG 2 cut(s) 128, 287
ZrmI AGTACT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.