RchiOBHm_Chr3g0475041

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0015657
Physical Location & Seq
Reverse (-)
20832770 .. 20835531
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGGAGTGGCTGCGTATCGTGGTGTTATTTTCGACGTTAGCAATTCATGTATCATGGGGTCTAGAGAGCACTACTGGTTCGTTACCGGAGCTGAGTCAACTGAGTCGGGAGTTGCTGGACTCGGCCAGGGAGCCCGAGTTCTTTGATTGGTTAAGAAAAGTCAGGAGGAAAATCCACGAGAACCCTGAGCTCGCCTTTGAGGAGGACGAGACGAGCCAATTCATAAGATCGGAGCTCGACTTGCTTGGGATAGAATACACGTGGCCGGTGGCCAAGACTGGAGTGGTTGCCTCCATCGGTTCTGGGTTGCAGCCATGGTTCGCTCTCAGGGCAGACATGGATGCTCTCCCAATTCAGGAATTGGTTGAGTGGGAGCACAAGAGCAAGAACCCCGGGAAGATGCATGCTTGTGGCCATGATGTTCATGTAACCATGCTGCTTGGAGCTGCTAAGTTACTTCAGCTCAAGAGTAAACAATTAAAGGGCACAGTCAAGCTTGTTTTTCAACCTGCAGAGGAGGGTCATGCAGGGGCATACCATATGTTAAAAGAGGTTGCTCTTGATGGAATTCAAGGCATTTTTGGATTACATGTTTCGCCAGAAATGCAGACTGGTTCAATTGGTTCTAGACCTGGTCCATTTCTTGCTGGTGCTGGTAGATTTTTAGTCACAGTTCAAGGAAAAGGTGGACATGCTGCAGCTCCCCATTTGACTGCAGACCCAATTCTTGCAGCCTGCTCGGTCATCCTTGCGCTCCAACAGATTGTATCTCGAGAAACCGACCCTCTAGAGGCCAGGGTGGTAACAGTTGGGTTTGTTAACGGTGGCCAAGCCGCAAATGTCATTCCAGAGACGGTGGAACTTAAAGGGACTTTTCGAAGCTTGACTTTAGATGGTATACATTACCTTCAGCAAAGGATTAAGGAGGTGATAGAGATGCAAGCATCAGTGCATCAGTGTAATGCAACAGTGGACTTCATGCATGAAAAAATGAGGCCGTATCCACCAACTGTAATTGATGAAGCACTCTATGTACACAGCAAGAGTGTTAGTGAAGCCTTGGTTGGGGAAGCAAATGTGTATCTTCTTCCAATGACTATGGGAGCAGAGGACTTTAGCTTCTATACACATAAAATGCCAGCCGCAATGTTCATGATCGGGACTACAGACCATACCTTGGGATCGAATGCTACAAGTTTGCATTCACCGTACTTGGTCATTGATGAGGAGGTACTTCCAATAGGAGCAGCTCTTCATGCTGCAGTTGCGATCTCGTTCTTGGATAACAATACTGATGCTGCTGCTGCGCACTCAGTCATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

441

Amino Acids

48.14

Weight (kDa)

5.57

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 108 - 423 2.6e-41 Peptidase family M20/M25/M40
M20_dimer PF07687 217 - 312 3.1e-13 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1308
Acc36I ACCTGC 1 cut(s) 517
AccB7I CCANNNNNTGG 1 cut(s) 1177
AccI GTMKAC 1 cut(s) 898
AciI CCGC 2 cut(s) 834, 1143
AclWI GGATC 1 cut(s) 1189
AcoI YGGCCR 5 cut(s) 123, 263, 270, 412, 826
AcsI RAATTY 1 cut(s) 567
AcuI CTGAAG 2 cut(s) 443, 893
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 3 cut(s) 1035, 1211, 1233
AfiI CCNNNNNNNGG 4 cut(s) 355, 790, 1065, 1177
AflIII ACRYGT 2 cut(s) 258, 589
AgsI TTSAA 4 cut(s) 506, 572, 618, 677
AjnI CCWGG 3 cut(s) 125, 631, 794
AjuI GAANNNNNNNTTGG 2 cut(s) 1047, 1079
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
Alw21I GWGCWC 4 cut(s) 71, 192, 237, 378
Alw26I GTCTC 2 cut(s) 203, 845
AlwI GGATC 1 cut(s) 1189
Ama87I CYCGRG 3 cut(s) 134, 392, 771
AoxI GGCC 7 cut(s) 123, 263, 270, 412, 792, 826, 995
ApoI RAATTY 1 cut(s) 567
AspLEI GCGC 2 cut(s) 754, 1309
AspS9I GGNCC 1 cut(s) 635
AsuC2I CCSGG 2 cut(s) 393, 394
AsuHPI GGTGA 2 cut(s) 940, 1197
AsuII TTCGAA 1 cut(s) 877
AvaI CYCGRG 3 cut(s) 134, 392, 771
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 488
BalI TGGCCA 3 cut(s) 272, 414, 828
BanII GRGCYC 3 cut(s) 135, 192, 237
BauI CACGAG 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 261
Bbv12I GWGCWC 4 cut(s) 71, 192, 237, 378
BccI CCATC 3 cut(s) 302, 557, 887
BceAI ACGGC 1 cut(s) 982
BcgI CGANNNNNNTGC 2 cut(s) 720, 754
BciT130I CCWGG 3 cut(s) 127, 633, 796
BciVI GTATCC 1 cut(s) 1011
BcnI CCSGG 2 cut(s) 393, 394
BcoDI GTCTC 2 cut(s) 203, 845
BfaI CTAG 4 cut(s) 62, 627, 788, 1324
BfmI CTRYAG 5 cut(s) 510, 696, 714, 1164, 1260
BfuAI ACCTGC 1 cut(s) 517
BfuI GTATCC 1 cut(s) 1011
Bme1390I CCNGG 5 cut(s) 127, 393, 394, 633, 796
Bme18I GGWCC 1 cut(s) 635
BmeT110I CYCGRG 3 cut(s) 134, 392, 771
BmgT120I GGNCC 1 cut(s) 635
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 5 cut(s) 127, 393, 394, 633, 796
BmsI GCATC 6 cut(s) 331, 390, 927, 953, 961, 1285
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 877
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 2 cut(s) 393, 394
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 7 cut(s) 126, 314, 391, 392, 795, 1059, 1176
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 790, 1065, 1177
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 3 cut(s) 79, 283, 616
Bse3DI GCAATG 1 cut(s) 1152
BseBI CCWGG 3 cut(s) 127, 633, 796
BseDI CCNNGG 7 cut(s) 126, 314, 391, 392, 795, 1059, 1176
BseGI GGATG 2 cut(s) 346, 744
BseLI CCNNNNNNNGG 4 cut(s) 355, 790, 1065, 1177
BseMI GCAATG 1 cut(s) 1152
BseMII CTCAG 5 cut(s) 83, 92, 177, 340, 1326
BseNI ACTGG 3 cut(s) 79, 283, 616
BseRI GAGGAG 3 cut(s) 215, 530, 1241
BseSI GKGCMC 1 cut(s) 488
BshFI GGCC 7 cut(s) 125, 265, 272, 414, 794, 828, 997
BsiHKAI GWGCWC 4 cut(s) 71, 192, 237, 378
BsiHKCI CYCGRG 3 cut(s) 134, 392, 771
BsiSI CCGG 3 cut(s) 86, 266, 393
BslFI GGGAC 2 cut(s) 883, 1174
BslI CCNNNNNNNGG 4 cut(s) 355, 790, 1065, 1177
BsmAI GTCTC 2 cut(s) 203, 845
BsmBI CGTCTC 2 cut(s) 203, 845
BsmFI GGGAC 2 cut(s) 883, 1174
BsmI GAATGC 2 cut(s) 1192, 1201
BsnI GGCC 7 cut(s) 125, 265, 272, 414, 794, 828, 997
BsoBI CYCGRG 3 cut(s) 134, 392, 771
Bsp119I TTCGAA 1 cut(s) 877
Bsp1286I GDGCHC 6 cut(s) 71, 135, 192, 237, 378, 488
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 4 cut(s) 227, 1155, 1181, 1269
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 2 cut(s) 834, 1143
BspANI GGCC 7 cut(s) 125, 265, 272, 414, 794, 828, 997
BspCNI CTCAG 5 cut(s) 84, 93, 178, 339, 1325
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 1 cut(s) 132
BspMAI CTGCAG 4 cut(s) 514, 700, 718, 1264
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 1 cut(s) 1189
BspQI GCTCTTC 1 cut(s) 1257
BspT104I TTCGAA 1 cut(s) 877
BsrDI GCAATG 1 cut(s) 1152
BsrFI RCCGGY 1 cut(s) 265
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 3 cut(s) 79, 283, 616
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 7 cut(s) 126, 314, 391, 392, 795, 1059, 1176
BssMI GATC 4 cut(s) 227, 1155, 1181, 1269
BssNAI GTATAC 1 cut(s) 899
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 3 cut(s) 314, 1059, 1176
Bst1107I GTATAC 1 cut(s) 899
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 127, 633, 796
Bst4CI ACNGT 8 cut(s) 490, 673, 808, 824, 856, 970, 1012, 1209
Bst6I CTCTTC 1 cut(s) 1257
BstAUI TGTACA 1 cut(s) 1033
BstBAI YACGTR 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 877
BstC8I GCNNGC 5 cut(s) 192, 405, 736, 942, 1140
BstDEI CTNAG 6 cut(s) 92, 101, 186, 326, 450, 1312
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 2 cut(s) 346, 744
BstHHI GCGC 2 cut(s) 754, 1309
BstKTI GATC 4 cut(s) 230, 1158, 1184, 1272
BstMAI GTCTC 2 cut(s) 203, 845
BstMBI GATC 4 cut(s) 227, 1155, 1181, 1269
BstMWI GCNNNNNNNGC 6 cut(s) 241, 329, 604, 1256, 1265, 1304
BstNI CCWGG 3 cut(s) 127, 633, 796
BstNSI RCATGY 3 cut(s) 407, 593, 695
BstSCI CCNGG 5 cut(s) 125, 391, 392, 631, 794
BstSFI CTRYAG 5 cut(s) 510, 696, 714, 1164, 1260
BstSLI GKGCMC 1 cut(s) 488
BstZ17I GTATAC 1 cut(s) 899
BsuI GTATCC 1 cut(s) 1011
BsuRI GGCC 7 cut(s) 125, 265, 272, 414, 794, 828, 997
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 2 cut(s) 346, 744
BtsIMutI CAGTG 3 cut(s) 954, 962, 975
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 5 cut(s) 192, 405, 736, 942, 1140
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 2 cut(s) 754, 1309
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 635
Cfr9I CCCGGG 1 cut(s) 392
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 3 cut(s) 1034, 1210, 1232
CviQI GTAC 3 cut(s) 1034, 1210, 1232
DdeI CTNAG 6 cut(s) 92, 101, 186, 326, 450, 1312
DpnI GATC 4 cut(s) 229, 1157, 1183, 1271
DpnII GATC 4 cut(s) 227, 1155, 1181, 1269
EaeI YGGCCR 5 cut(s) 123, 263, 270, 412, 826
Eam1104I CTCTTC 1 cut(s) 1257
EarI CTCTTC 1 cut(s) 1257
Ecl136II GAGCTC 2 cut(s) 190, 235
Eco130I CCWWGG 3 cut(s) 314, 1059, 1176
Eco24I GRGCYC 3 cut(s) 135, 192, 237
Eco47I GGWCC 1 cut(s) 635
Eco53kI GAGCTC 2 cut(s) 190, 235
Eco57I CTGAAG 2 cut(s) 443, 893
Eco72I CACGTG 1 cut(s) 261
Eco88I CYCGRG 3 cut(s) 134, 392, 771
EcoICRI GAGCTC 2 cut(s) 190, 235
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 3 cut(s) 125, 631, 794
EcoT14I CCWWGG 3 cut(s) 314, 1059, 1176
EcoT22I ATGCAT 2 cut(s) 405, 984
EcoT38I GRGCYC 3 cut(s) 135, 192, 237
ErhI CCWWGG 3 cut(s) 314, 1059, 1176
Esp3I CGTCTC 2 cut(s) 203, 845
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 2 cut(s) 883, 1174
FauNDI CATATG 1 cut(s) 540
FblI GTMKAC 1 cut(s) 898
FokI GGATG 2 cut(s) 353, 731
FriOI GRGCYC 3 cut(s) 135, 192, 237
FspBI CTAG 4 cut(s) 62, 627, 788, 1324
FspI TGCGCA 1 cut(s) 1308
GlaI GCGC 2 cut(s) 753, 1308
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 7 cut(s) 125, 265, 272, 414, 794, 828, 997
HapII CCGG 3 cut(s) 86, 266, 393
HhaI GCGC 2 cut(s) 754, 1309
Hin6I GCGC 2 cut(s) 752, 1307
HinP1I GCGC 2 cut(s) 752, 1307
HincII GTYRAC 2 cut(s) 98, 820
HindII GTYRAC 2 cut(s) 98, 820
HindIII AAGCTT 2 cut(s) 494, 880
HinfI GANTC 3 cut(s) 94, 103, 119
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 3 cut(s) 86, 266, 393
HphI GGTGA 2 cut(s) 940, 1197
Hpy166II GTNNAC 7 cut(s) 98, 473, 689, 820, 899, 973, 1036
Hpy188I TCNGA 1 cut(s) 232
Hpy8I GTNNAC 7 cut(s) 98, 473, 689, 820, 899, 973, 1036
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 917
HpyCH4III ACNGT 8 cut(s) 490, 673, 808, 824, 856, 970, 1012, 1209
HpyCH4IV ACGT 2 cut(s) 35, 260
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 329, 604, 1256, 1265, 1304
HpyF3I CTNAG 6 cut(s) 92, 101, 186, 326, 450, 1312
HpySE526I ACGT 2 cut(s) 35, 260
HspAI GCGC 2 cut(s) 752, 1307
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 4 cut(s) 227, 1155, 1181, 1269
LguI GCTCTTC 1 cut(s) 1257
LmnI GCTCC 9 cut(s) 88, 130, 232, 373, 443, 706, 759, 1103, 1244
LweI GCATC 6 cut(s) 331, 390, 927, 953, 961, 1285
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 4 cut(s) 62, 627, 788, 1324
MaeII ACGT 2 cut(s) 35, 260
MaeIII GTNAC 5 cut(s) 81, 427, 453, 667, 802
MalI GATC 4 cut(s) 229, 1157, 1183, 1271
MboI GATC 4 cut(s) 227, 1155, 1181, 1269
MboII GAAGA 4 cut(s) 409, 1076, 1079, 1244
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 6 cut(s) 71, 135, 192, 237, 378, 488
MlsI TGGCCA 3 cut(s) 272, 414, 828
MluCI AATT 9 cut(s) 42, 218, 351, 359, 476, 567, 618, 723, 1014
MluNI TGGCCA 3 cut(s) 272, 414, 828
MlyI GAGTC 3 cut(s) 103, 112, 113
MmeI TCCRAC 1 cut(s) 781
Mox20I TGGCCA 3 cut(s) 272, 414, 828
Mph1103I ATGCAT 2 cut(s) 405, 984
MscI TGGCCA 3 cut(s) 272, 414, 828
MseI TTAA 6 cut(s) 152, 479, 545, 819, 864, 921
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 3 cut(s) 272, 414, 828
MspI CCGG 3 cut(s) 86, 266, 393
MspR9I CCNGG 5 cut(s) 127, 393, 394, 633, 796
MunI CAATTG 1 cut(s) 618
Mva1269I GAATGC 2 cut(s) 1192, 1201
MvaI CCWGG 3 cut(s) 127, 633, 796
MwoI GCNNNNNNNGC 6 cut(s) 241, 329, 604, 1256, 1265, 1304
NciI CCSGG 2 cut(s) 393, 394
NcoI CCATGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 540
NdeII GATC 4 cut(s) 227, 1155, 1181, 1269
NlaIV GGNNCC 1 cut(s) 132
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 667
NsbI TGCGCA 1 cut(s) 1308
NsiI ATGCAT 2 cut(s) 405, 984
NspI RCATGY 3 cut(s) 407, 593, 695
NspV TTCGAA 1 cut(s) 877
PaeI GCATGC 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 771
PagI TCATGA 1 cut(s) 1152
PciI ACATGT 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 1257
PctI GAATGC 2 cut(s) 1192, 1201
PflFI GACNNNGTC 1 cut(s) 633
PflMI CCANNNNNTGG 1 cut(s) 1177
PleI GAGTC 3 cut(s) 102, 111, 113
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 3 cut(s) 102, 111, 113
Ppu21I YACGTR 1 cut(s) 261
PscI ACATGT 1 cut(s) 589
Psp124BI GAGCTC 2 cut(s) 192, 237
Psp6I CCWGG 3 cut(s) 125, 631, 794
PspCI CACGTG 1 cut(s) 261
PspGI CCWGG 3 cut(s) 125, 631, 794
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 635
PstI CTGCAG 4 cut(s) 514, 700, 718, 1264
PsyI GACNNNGTC 1 cut(s) 633
RsaI GTAC 3 cut(s) 1035, 1211, 1233
RsaNI GTAC 3 cut(s) 1034, 1210, 1232
RseI CAYNNNNRTG 1 cut(s) 408
SacI GAGCTC 2 cut(s) 192, 237
SapI GCTCTTC 1 cut(s) 1257
SaqAI TTAA 6 cut(s) 152, 479, 545, 819, 864, 921
Sau3AI GATC 4 cut(s) 227, 1155, 1181, 1269
Sau96I GGNCC 1 cut(s) 635
SchI GAGTC 3 cut(s) 103, 112, 113
ScrFI CCNGG 5 cut(s) 127, 393, 394, 633, 796
SduI GDGCHC 6 cut(s) 71, 135, 192, 237, 378, 488
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 6 cut(s) 331, 390, 927, 953, 961, 1285
SfcI CTRYAG 5 cut(s) 510, 696, 714, 1164, 1260
Sfr274I CTCGAG 1 cut(s) 771
SfuI TTCGAA 1 cut(s) 877
SinI GGWCC 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 771
SmaI CCCGGG 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 464, 771
SmoI CTYRAG 2 cut(s) 464, 771
SphI GCATGC 1 cut(s) 407
Sse9I AATT 9 cut(s) 42, 218, 351, 359, 476, 567, 618, 723, 1014
SsiI CCGC 2 cut(s) 834, 1143
SspMI CTAG 4 cut(s) 62, 627, 788, 1324
SstI GAGCTC 2 cut(s) 192, 237
StyD4I CCNGG 5 cut(s) 125, 391, 392, 631, 794
StyI CCWWGG 3 cut(s) 314, 1059, 1176
TaaI ACNGT 8 cut(s) 490, 673, 808, 824, 856, 970, 1012, 1209
TaiI ACGT 2 cut(s) 38, 263
TaqI TCGA 5 cut(s) 32, 237, 772, 877, 1184
TaqII GACCGA 1 cut(s) 730
TasI AATT 9 cut(s) 42, 218, 351, 359, 476, 567, 618, 723, 1014
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 2 cut(s) 836, 1145
Tru1I TTAA 6 cut(s) 152, 479, 545, 819, 864, 921
Tru9I TTAA 6 cut(s) 152, 479, 545, 819, 864, 921
TscAI CASTG 3 cut(s) 954, 962, 975
TseFI GTSAC 1 cut(s) 667
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 8 cut(s) 35, 211, 413, 967, 999, 1035, 1141, 1244
TspMI CCCGGG 1 cut(s) 392
TspRI CASTG 3 cut(s) 954, 962, 975
Tth111I GACNNNGTC 1 cut(s) 633
Van91I CCANNNNNTGG 1 cut(s) 1177
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 1 cut(s) 567
XbaI TCTAGA 3 cut(s) 61, 626, 787
XceI RCATGY 3 cut(s) 407, 593, 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 771
XmaI CCCGGG 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 898
XspI CTAG 4 cut(s) 62, 627, 788, 1324
Zsp2I ATGCAT 2 cut(s) 405, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.