RchiOBHm_Chr3g0476871

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016402
Physical Location & Seq
Reverse (-)
22836212 .. 22838597
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2139 bp
ATGGATAAAATTAGGGAGATACCATTTTTCTCCAAAGAGGTGAAGAAGATATGGGACGTGTGGAATCTGCAGGGCTGTATTCTCTTAAGTCTCTCACTGCAAGTTTCTCTGCTCTTGGCATCATTTCGGAAAAAATGCAGAAGCAAATTGCTACGAGGACTCATCTGGTCTGCGTATTTGCTCGCCGATTGGATTGCTGCTGTTGCAATAGGGATAATCACCAAATCCCAAGTTGACACTTGCGACCCGAAGGGCAACAAAGATCTTTTGGCATTCTGGGCTTCCTTTCTTCTACTCCATCTTGGCGGCCCTGATACCATTACCTCTTTTGCTCTTGAGGACAACGAGTTCTGGGTTAGGCACTTGGTTGGACTAATTGTTCAAGTTCTTGCAGCTGCTTGTTGTTTCTACCTGACACTTCATAACAAGTTCTTGGTTCCGACAGTACTAGTCTTTGTTGTTGGAACAATCAAGTTTGGGGAGAGAACTTGTGCTCTGTATCTTGCTAGTCTAGACCATTTTGGGGAGACTGTATTGCCAAAGCCGGTGGCTGGACCTGACTATGAAGAAACTGTAGCAATCCATACTTCAATGAGGTCAGTCGCAGTCTCTACGACACAAGAAGTAGTGGTACCAATCAGGCCATATATTGGGGATGCACATAAAGATCCAAAGTTTACGATTAAGGGAAATTCAAAAGAATCTTTAAACGACATGCAATTGCTGCAAGAAGCGTATTACCTCTTTGAGAGCTTCAAAAGACTCATTGCTGGCTTTCTTCTCAGCTCCAAAGACCGAGAACTGAGTCGAGATTTCTTCCTTAAAAGAACCTATACTAGCGCTTTTCAGTTGATAGAGTACGAGCTCAACTTCATGTATGAGGTTCTTCACACCAAGGTTATTGTGGTGCGTCGCAAAATTGGGTACACCCTCCGCTTAATAAGCTTCTGTGCAACCATTGTTGCCTTGGTGTTCTTCTTTTCGGTTGAAAAGAAAGAGTTTGTCAAGTTTGACATTGATCTTACTTATGCTTTGTTTAGTGGAGCCATTTTCCTGGACATCATATCTGTCTTTATGCTTATCTTCTCTGACTGGACCCTCATTGCCTTCAACAACACTAACATTTGGAGCAGATTTATTGCTGCTGTAATAATGAAAAGAGGAAGGTGGTCTGGATCGGTATCCCAATACAATATGATAAAGAATTGCCTGGATGAGCGTCCAGAATGTGTCTTTACTCTTGCCGGTTGTCTGGGTGTTGGGGGAATTCTGGGCAGAACCAAAATTGTCTACTTTTCATCCTCAGAAGCAGTCACCGAAGAGCTGAAGTACTTTATCTTCAGGCAGCTGAGGTTAAAATCTCTGAAAGCAAACAACAGAAGGGATGGAATGGAGGCATGTTCACAAAGAGGTGACTGGGCTCTGCTGCTGAATTTAAACTATCAGAAACTCAAATGGAGCATTGGGGATTATCAATATGCGGAAAGTGTTCTTCTCTGGCACATTGCAACAGAACTCTACTATGCTGAAAGTGGAACCGAGCAATGTCCTGATTGTCGTCAAGGTAGACAAGCAACACAAAGGGAATGTGATAGTGACAATCGACTAAAACTTTGCAATTTTTGTGAAAGTAACGCCAGGCGTAGTATTTGCAAACTTGTTTCAGATTATCTGTTTTATCTTCTGGTGATGCAGCATCCAATGATGGCCCCAGTTTTGGGGAATTGGCATATAGTGTTCCAGGACACTTGTGCAGAGGCCAAGAGATTCTTTCGCAAGCATTCAGTAAAAGATCATAAGGAAGCCTGTAAAAAGATTTCTGGTGTCGAAGCCAAATTCAGATCGGCTGCTGTGAAGGGAAGTAAAAGCAAATCTGTGCTGTTTGATGCATGTATGCTTGCAGAACAGCTTAAGGAGTTGGAGGCAGATCATGACCCATGGAAGCTAATGAGCCGGGTGTGGGTGGAATTATTGTCATATGCTTCCATTAAATGTAGACCGATTGTACATGCTCAGCAACCAAGTAGGGGTGGAGAACTGATGACTTTCACTTGGCTACTGATGAATCATCTGGGCTTGGGAACACAATTCTACGAGCTGGAAAACCAAGCTGGAACAAAAATGGTGGCAGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

712

Amino Acids

81.41

Weight (kDa)

8.82

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 56 - 401 2.3e-92 Domain of unknown function (DUF4220)
DUF594 PF04578 642 - 693 1.5e-17 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016402)

Species Orthologous Gene IDs
malus_domestica MD10G1305500.v1.1
prunus_persica Prupe.4G034200_v2.0.a1
pyrus_communis pycom10g25790
rosa_chinensis RchiOBHm_Chr3g0476871
rosa_laevigata RLG00000023747
rosa_multiflora Rmu_sc0000288.1_g000035
rosa_roxburghii Rroxscaffold_6G00404500
rosa_rugosa Rorug03G0157400
rosa_samantha Rh3AG207500 Rh3BG240100 Rh3CG233800 Rh3CG234900 Rh3DG233800
rosa_wichuraiana Rw0G011130 Rw3G018890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 631
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 3 cut(s) 1290, 1567, 1996
AciI CCGC 3 cut(s) 306, 934, 1481
AclWI GGATC 2 cut(s) 662, 1183
AcsI RAATTY 4 cut(s) 691, 1266, 1432, 1835
AcuI CTGAAG 2 cut(s) 1324, 1346
AfaI GTAC 6 cut(s) 447, 633, 860, 926, 1331, 2007
AfeI AGCGCT 1 cut(s) 841
AfiI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
AflII CTTAAG 2 cut(s) 85, 1910
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 6 cut(s) 383, 591, 696, 757, 989, 1111
AhlI ACTAGT 1 cut(s) 448
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 4 cut(s) 1053, 1209, 1637, 1740
AloI GAACNNNNNNTCC 4 cut(s) 332, 363, 364, 395
Alw21I GWGCWC 2 cut(s) 496, 867
Alw26I GTCTC 3 cut(s) 95, 521, 613
AlwI GGATC 2 cut(s) 662, 1183
Aor51HI AGCGCT 1 cut(s) 841
AoxI GGCC 4 cut(s) 307, 641, 1707, 1758
ApeKI GCWGC 9 cut(s) 197, 392, 395, 724, 1142, 1345, 1426, 1693, 1847
ApoI RAATTY 4 cut(s) 691, 1266, 1432, 1835
ArsI GACNNNNNNTTYG 2 cut(s) 1596, 1628
Asp700I GAANNNNTTC 1 cut(s) 1488
Asp718I GGTACC 1 cut(s) 631
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 4 cut(s) 308, 554, 1095, 1708
AsuC2I CCSGG 1 cut(s) 1955
AsuHPI GGTGA 5 cut(s) 52, 211, 1306, 1424, 1699
AvaII GGWCC 2 cut(s) 554, 1095
BanI GGYRCC 1 cut(s) 631
BanII GRGCYC 2 cut(s) 867, 1423
BarI GAAGNNNNNNTAC 4 cut(s) 615, 647, 1322, 1354
Bbv12I GWGCWC 2 cut(s) 496, 867
BbvCI CCTCAGC 1 cut(s) 1349
BbvI GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
BccI CCATC 3 cut(s) 306, 1379, 1699
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 4 cut(s) 1055, 1211, 1639, 1742
BciVI GTATCC 1 cut(s) 1192
BcnI CCSGG 1 cut(s) 1955
BcoDI GTCTC 3 cut(s) 95, 521, 613
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 4 cut(s) 449, 507, 512, 837
BfmI CTRYAG 2 cut(s) 68, 573
BfoI RGCGCY 1 cut(s) 843
BfrI CTTAAG 2 cut(s) 85, 1910
BfuI GTATCC 1 cut(s) 1192
BglII AGATCT 1 cut(s) 262
BlpI GCTNAGC 1 cut(s) 2013
BmcAI AGTACT 2 cut(s) 447, 1331
Bme1390I CCNGG 5 cut(s) 1055, 1211, 1639, 1742, 1955
Bme18I GGWCC 2 cut(s) 554, 1095
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 4 cut(s) 308, 554, 1095, 1708
BmiI GGNNCC 6 cut(s) 438, 633, 1045, 1097, 1537, 1710
BmrFI CCNGG 5 cut(s) 1055, 1211, 1639, 1742, 1955
BmrI ACTGGG 2 cut(s) 1426, 1706
BmsI GCATC 5 cut(s) 128, 646, 1680, 1705, 1876
BmuI ACTGGG 2 cut(s) 1426, 1706
Bpu10I CCTNAGC 1 cut(s) 1349
Bpu1102I GCTNAGC 1 cut(s) 2013
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 1955
BsaBI GATNNNNATC 1 cut(s) 1180
BsaJI CCNNGG 3 cut(s) 894, 966, 1937
Bsc4I CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
Bse118I RCCGGY 2 cut(s) 544, 1244
Bse1I ACTGG 3 cut(s) 1097, 1421, 1712
Bse3DI GCAATG 4 cut(s) 765, 1101, 1503, 1550
Bse8I GATNNNNATC 1 cut(s) 1180
BseBI CCWGG 4 cut(s) 1055, 1211, 1639, 1742
BseDI CCNNGG 3 cut(s) 894, 966, 1937
BseGI GGATG 5 cut(s) 661, 1219, 1298, 1390, 1696
BseJI GATNNNNATC 1 cut(s) 1180
BseLI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
BseMI GCAATG 4 cut(s) 765, 1101, 1503, 1550
BseMII CTCAG 5 cut(s) 794, 796, 1317, 1340, 2027
BseNI ACTGG 3 cut(s) 1097, 1421, 1712
BseXI GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
BsgI GTGCAG 1 cut(s) 1773
BshFI GGCC 4 cut(s) 309, 643, 1709, 1760
BshNI GGYRCC 1 cut(s) 631
BsiHKAI GWGCWC 2 cut(s) 496, 867
BsiSI CCGG 3 cut(s) 545, 1245, 1954
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 7 cut(s) 523, 551, 650, 1717, 1718, 1960, 2027
BsmAI GTCTC 3 cut(s) 95, 521, 613
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 2 cut(s) 272, 1780
BsnI GGCC 4 cut(s) 309, 643, 1709, 1760
Bsp1286I GDGCHC 3 cut(s) 496, 867, 1423
Bsp1407I TGTACA 1 cut(s) 2005
Bsp143I GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
Bsp1720I GCTNAGC 1 cut(s) 2013
Bsp19I CCATGG 1 cut(s) 1937
BspACI CCGC 3 cut(s) 306, 934, 1481
BspANI GGCC 4 cut(s) 309, 643, 1709, 1760
BspCNI CTCAG 5 cut(s) 795, 795, 1316, 1341, 2026
BspHI TCATGA 1 cut(s) 1930
BspLI GGNNCC 6 cut(s) 438, 633, 1045, 1097, 1537, 1710
BspMAI CTGCAG 1 cut(s) 72
BspPI GGATC 2 cut(s) 662, 1183
BspQI GCTCTTC 1 cut(s) 1314
BspT107I GGYRCC 1 cut(s) 631
BspTI CTTAAG 2 cut(s) 85, 1910
BsrDI GCAATG 4 cut(s) 765, 1101, 1503, 1550
BsrFI RCCGGY 2 cut(s) 544, 1244
BsrGI TGTACA 1 cut(s) 2005
BsrI ACTGG 3 cut(s) 1097, 1421, 1712
BssAI RCCGGY 2 cut(s) 544, 1244
BssECI CCNNGG 3 cut(s) 894, 966, 1937
BssMI GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
BssT1I CCWWGG 3 cut(s) 894, 966, 1937
Bst2UI CCWGG 4 cut(s) 1055, 1211, 1639, 1742
Bst4CI ACNGT 3 cut(s) 445, 532, 574
Bst6I CTCTTC 1 cut(s) 1314
BstAFI CTTAAG 2 cut(s) 85, 1910
BstAPI GCANNNNNTGC 1 cut(s) 724
BstAUI TGTACA 1 cut(s) 2005
BstC8I GCNNGC 4 cut(s) 183, 772, 1778, 1899
BstDEI CTNAG 5 cut(s) 782, 803, 1303, 1349, 2013
BstDSI CCRYGG 1 cut(s) 1937
BstF5I GGATG 5 cut(s) 661, 1219, 1298, 1390, 1696
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 7 cut(s) 265, 670, 1021, 1178, 1795, 1844, 1930
BstMAI GTCTC 3 cut(s) 95, 521, 613
BstMBI GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
BstMWI GCNNNNNNNGC 4 cut(s) 203, 278, 724, 942
BstNI CCWGG 4 cut(s) 1055, 1211, 1639, 1742
BstNSI RCATGY 4 cut(s) 718, 1401, 1893, 2012
BstSCI CCNGG 5 cut(s) 1053, 1209, 1637, 1740, 1953
BstSFI CTRYAG 2 cut(s) 68, 573
BstV1I GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
BstX2I RGATCY 2 cut(s) 262, 667
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 262, 667
BsuI GTATCC 1 cut(s) 1192
BsuRI GGCC 4 cut(s) 309, 643, 1709, 1760
BtgI CCRYGG 1 cut(s) 1937
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 5 cut(s) 661, 1219, 1298, 1390, 1696
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 4 cut(s) 183, 772, 1778, 1899
CciI TCATGA 1 cut(s) 1930
CfoI GCGC 1 cut(s) 842
Cfr10I RCCGGY 2 cut(s) 544, 1244
Cfr13I GGNCC 4 cut(s) 308, 554, 1095, 1708
CseI GACGC 2 cut(s) 899, 1208
Csp6I GTAC 6 cut(s) 446, 632, 859, 925, 1330, 2006
CspCI CAANNNNNGTGG 3 cut(s) 528, 563, 2106
CviAII CATG 7 cut(s) 715, 874, 1398, 1890, 1931, 1938, 2009
CviQI GTAC 6 cut(s) 446, 632, 859, 925, 1330, 2006
DdeI CTNAG 5 cut(s) 782, 803, 1303, 1349, 2013
DpnI GATC 7 cut(s) 264, 669, 1020, 1177, 1794, 1843, 1929
DpnII GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
DraI TTTAAA 2 cut(s) 708, 1437
Eam1104I CTCTTC 1 cut(s) 1314
EarI CTCTTC 1 cut(s) 1314
Ecl136II GAGCTC 1 cut(s) 865
Eco130I CCWWGG 3 cut(s) 894, 966, 1937
Eco24I GRGCYC 2 cut(s) 867, 1423
Eco47I GGWCC 2 cut(s) 554, 1095
Eco47III AGCGCT 1 cut(s) 841
Eco53kI GAGCTC 1 cut(s) 865
Eco57I CTGAAG 2 cut(s) 1324, 1346
EcoICRI GAGCTC 1 cut(s) 865
EcoRI GAATTC 1 cut(s) 1266
EcoRII CCWGG 4 cut(s) 1053, 1209, 1637, 1740
EcoT14I CCWWGG 3 cut(s) 894, 966, 1937
EcoT22I ATGCAT 1 cut(s) 1891
EcoT38I GRGCYC 2 cut(s) 867, 1423
ErhI CCWWGG 3 cut(s) 894, 966, 1937
FaeI CATG 7 cut(s) 718, 877, 1401, 1893, 1934, 1941, 2012
FalI AAGNNNNNCTT 2 cut(s) 1754, 1786
FaqI GGGAC 1 cut(s) 68
FatI CATG 7 cut(s) 714, 873, 1397, 1889, 1930, 1937, 2008
FauNDI CATATG 1 cut(s) 1978
FblI GTMKAC 3 cut(s) 1290, 1567, 1996
FokI GGATG 5 cut(s) 668, 1226, 1285, 1397, 1683
FriOI GRGCYC 2 cut(s) 867, 1423
FspBI CTAG 4 cut(s) 449, 507, 512, 837
GlaI GCGC 1 cut(s) 841
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 4 cut(s) 309, 643, 1709, 1760
HapII CCGG 3 cut(s) 545, 1245, 1954
HgaI GACGC 2 cut(s) 899, 1208
HhaI GCGC 1 cut(s) 842
Hin1II CATG 7 cut(s) 718, 877, 1401, 1893, 1934, 1941, 2012
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 7 cut(s) 64, 159, 701, 762, 805, 1767, 2065
HpaII CCGG 3 cut(s) 545, 1245, 1954
HphI GGTGA 5 cut(s) 52, 211, 1306, 1424, 1699
Hpy166II GTNNAC 7 cut(s) 235, 678, 927, 1291, 1403, 1568, 1997
Hpy188I TCNGA 8 cut(s) 129, 441, 1090, 1306, 1365, 1446, 1666, 1841
Hpy188III TCNNGA 7 cut(s) 335, 512, 809, 1173, 1223, 1550, 1931
Hpy8I GTNNAC 7 cut(s) 235, 678, 927, 1291, 1403, 1568, 1997
Hpy99I CGWCG 1 cut(s) 915
HpyAV CCTTC 5 cut(s) 244, 1117, 1158, 1374, 1849
HpyCH4III ACNGT 3 cut(s) 445, 532, 574
HpyCH4IV ACGT 1 cut(s) 57
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 278, 724, 942
HpyF3I CTNAG 5 cut(s) 782, 803, 1303, 1349, 2013
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 7 cut(s) 718, 877, 1401, 1893, 1934, 1941, 2012
HspAI GCGC 1 cut(s) 840
KpnI GGTACC 1 cut(s) 635
Kzo9I GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 4 cut(s) 791, 1043, 1128, 1458
Lsp1109I GCAGC 9 cut(s) 184, 382, 404, 711, 1129, 1357, 1413, 1705, 1834
LweI GCATC 5 cut(s) 128, 646, 1680, 1705, 1876
MaeI CTAG 4 cut(s) 449, 507, 512, 837
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 4 cut(s) 1312, 1412, 1595, 1631
MalI GATC 7 cut(s) 264, 669, 1020, 1177, 1794, 1843, 1929
MboI GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 2 cut(s) 262, 667
MhlI GDGCHC 3 cut(s) 496, 867, 1423
MlyI GAGTC 3 cut(s) 153, 756, 814
MmeI TCCRAC 4 cut(s) 349, 442, 464, 1899
Mph1103I ATGCAT 1 cut(s) 1891
MroXI GAANNNNTTC 1 cut(s) 1488
MseI TTAA 9 cut(s) 86, 684, 707, 822, 938, 1355, 1436, 1911, 1989
MspA1I CMGCKG 2 cut(s) 395, 1348
MspCI CTTAAG 2 cut(s) 85, 1910
MspI CCGG 3 cut(s) 545, 1245, 1954
MspR9I CCNGG 5 cut(s) 1055, 1211, 1639, 1742, 1955
MunI CAATTG 1 cut(s) 719
Mva1269I GAATGC 2 cut(s) 272, 1780
MvaI CCWGG 4 cut(s) 1055, 1211, 1639, 1742
MwoI GCNNNNNNNGC 4 cut(s) 203, 278, 724, 942
NciI CCSGG 1 cut(s) 1955
NcoI CCATGG 1 cut(s) 1937
NdeI CATATG 1 cut(s) 1978
NdeII GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
NlaIII CATG 7 cut(s) 718, 877, 1401, 1893, 1934, 1941, 2012
NlaIV GGNNCC 6 cut(s) 438, 633, 1045, 1097, 1537, 1710
NmuCI GTSAC 3 cut(s) 1312, 1412, 1595
NsiI ATGCAT 1 cut(s) 1891
NspI RCATGY 4 cut(s) 718, 1401, 1893, 2012
PagI TCATGA 1 cut(s) 1930
PciSI GCTCTTC 1 cut(s) 1314
PctI GAATGC 2 cut(s) 272, 1780
PdmI GAANNNNTTC 1 cut(s) 1488
PfeI GAWTC 4 cut(s) 64, 701, 1767, 2065
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 2 cut(s) 1053, 1740
PleI GAGTC 3 cut(s) 153, 756, 813
PpsI GAGTC 3 cut(s) 153, 756, 813
Psp124BI GAGCTC 1 cut(s) 867
Psp6I CCWGG 4 cut(s) 1053, 1209, 1637, 1740
PspGI CCWGG 4 cut(s) 1053, 1209, 1637, 1740
PspN4I GGNNCC 6 cut(s) 438, 633, 1045, 1097, 1537, 1710
PspPI GGNCC 4 cut(s) 308, 554, 1095, 1708
PstI CTGCAG 1 cut(s) 72
PsuI RGATCY 2 cut(s) 262, 667
PvuII CAGCTG 2 cut(s) 395, 1348
RsaI GTAC 6 cut(s) 447, 633, 860, 926, 1331, 2007
RsaNI GTAC 6 cut(s) 446, 632, 859, 925, 1330, 2006
SacI GAGCTC 1 cut(s) 867
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 9 cut(s) 86, 684, 707, 822, 938, 1355, 1436, 1911, 1989
Sau3AI GATC 7 cut(s) 262, 667, 1018, 1175, 1792, 1841, 1927
Sau96I GGNCC 4 cut(s) 308, 554, 1095, 1708
ScaI AGTACT 2 cut(s) 447, 1331
SchI GAGTC 3 cut(s) 153, 756, 814
ScrFI CCNGG 5 cut(s) 1055, 1211, 1639, 1742, 1955
SduI GDGCHC 3 cut(s) 496, 867, 1423
SfaNI GCATC 5 cut(s) 128, 646, 1680, 1705, 1876
SfcI CTRYAG 2 cut(s) 68, 573
SinI GGWCC 2 cut(s) 554, 1095
SmlI CTYRAG 3 cut(s) 85, 335, 1910
SmoI CTYRAG 3 cut(s) 85, 335, 1910
SpeI ACTAGT 1 cut(s) 448
SsiI CCGC 3 cut(s) 306, 934, 1481
SspMI CTAG 4 cut(s) 449, 507, 512, 837
SstI GAGCTC 1 cut(s) 867
StyD4I CCNGG 5 cut(s) 1053, 1209, 1637, 1740, 1953
StyI CCWWGG 3 cut(s) 894, 966, 1937
TaaI ACNGT 3 cut(s) 445, 532, 574
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 808, 1603, 1827
TaqII GACCGA 2 cut(s) 810, 2014
TatI WGTACW 3 cut(s) 445, 1329, 2005
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 4 cut(s) 64, 701, 1767, 2065
Tru1I TTAA 9 cut(s) 86, 684, 707, 822, 938, 1355, 1436, 1911, 1989
Tru9I TTAA 9 cut(s) 86, 684, 707, 822, 938, 1355, 1436, 1911, 1989
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 3 cut(s) 1312, 1412, 1595
TseI GCWGC 9 cut(s) 197, 392, 395, 724, 1142, 1345, 1426, 1693, 1847
Tsp45I GTSAC 3 cut(s) 1312, 1412, 1595
TspDTI ATGAA 6 cut(s) 410, 579, 862, 1169, 1287, 2078
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 650
Vha464I CTTAAG 2 cut(s) 85, 1910
VpaK11BI GGWCC 2 cut(s) 554, 1095
XapI RAATTY 4 cut(s) 691, 1266, 1432, 1835
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 4 cut(s) 718, 1401, 1893, 2012
XcmI CCANNNNNNNNNTGG 2 cut(s) 901, 964
XmiI GTMKAC 3 cut(s) 1290, 1567, 1996
XmnI GAANNNNTTC 1 cut(s) 1488
XspI CTAG 4 cut(s) 449, 507, 512, 837
ZrmI AGTACT 2 cut(s) 447, 1331
Zsp2I ATGCAT 1 cut(s) 1891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.