Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013384

Physical Location & Seq

Genomic Coordinates Reverse (-)

43120185 .. 43122800

Gene Structure 2616 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1191 bp

ATGGCGCTTGTGAAACCCATCACGAATTTCGGCAATGTCGTTACTCCCAAATTTGGGAATACGAGAAGCACTAGTAGCAAGACGTCGAGCACCATGATCAGAATGTCGTCGTCCACCAAAAAGTCCAGTACTAAGAAAGCCATCAAGGAGACACTGTTGGCACCAAGGTTCTACACCACCGACTTTGATGAGATGGAGACCCTTTTCAACACCGAAATCAACAACAATTTGAACCAGGTTGAGTTTGAGGCTCTGCTTCAGGAGTTCAAGACCGACTACAACCAGACCCATTTTGTGAGGAACAAGGAGTTCAAGGTGGCTGCTGATAATCTCCAGGGCCCCCTCAGACAGATCTTTGTCGAGTTCTTGGAGAGGTCTTGTACTGCTGAGTTCTCTGGCTTCCTTCTTTACAAAGAGCTTGGAAGAAGGCTTAAGAAAACCAATCCAGTGGTAGCTGAGATATTCTCTCTCATGTCCAGGGATGAAGCCAGGCATGCCGGTTTCTTGAACAAAGGCCTGTCGGATTTCAATTTGGCATTGGACTTGGGGTTTCTGACCAAGGCAAGAAAATACACATTTTTCAAACCAAAGTTCATCTTCTATGCCACTTATTTGTCTGAGAAAATTGGATACTGGAGATACATCACCATATACAGGCATCTCAAGGCAAATCCCGAGTACCAGTGCTATCCCATTTTCAAGTATTTTGAGAATTGGTGCCAGGATGAGAACAGACATGGTGACTTCTTTTCTGCACTCATGAAGGCGCAACCCCAGTTCCTAAATGACTGGAAGGCAAAGTTGTGGTCTCGATTCTTCTGCTTATCGGTATATGTGACAATGTACCTAAATGATTGCCAACGGACAGCTTTCTATGAAGGCATCGGGCTAAATACAAAAGAATTTGATATGCATGTCATCATTGAGACAAATCGCACAACAGCCAGAATTTTCCCAGCCGTGCTTGATGTTGAGAACCCAGAATTCAAAAGGAAACTTGACCGCATGGTGGAGATTAACCAGAAGCTCATTGCTGTTGGGGAGAGCGACGACATTCCCCTGGTGAAAAATTTGAAGAGGATACCAATTGTTGCTGCATTGGCCTCTGAACTTCTGGCTGCGTACTTAATGCCACCCATTGAGTCTGGATCCGTCGATTTTGCAGAGTTCGAGCCCCAAGTGGTGTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004497 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009526 GO:0009528 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009657 GO:0009658 GO:0009706 GO:0009941 GO:0009987 GO:0015994 GO:0015995 GO:0016020 GO:0016043 GO:0016491 GO:0016705 GO:0016709 GO:0018130 GO:0019222 GO:0019438 GO:0019866 GO:0031090 GO:0031323 GO:0031967 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0033013 GO:0033014 GO:0034357 GO:0034641 GO:0042170 GO:0042440 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046483 GO:0048529 GO:0050789 GO:0050794 GO:0051171 GO:0051186 GO:0051188 GO:0055035 GO:0055114 GO:0065007 GO:0071704 GO:0071840 GO:0097159 GO:1901360 GO:1901362 GO:1901363 GO:1901401 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

Rubrerythrin

Protein Analysis

396

Amino Acids

46.05

Weight (kDa)

8.82

Isoelectric Point (pI)

34.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rubrerythrin	PF02915	121 - 254	3.6e-43	Rubrerythrin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013384)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56940 AT3G56940
fragaria_vesca	FvH4_1g28960 FvH4_1g28960
malus_domestica	MD00G1107700.v1.1
prunus_persica	Prupe.7G022500_v2.0.a1
pyrus_communis	pycom12g09220
rosa_chinensis	RchiOBHm_Chr3g0494361
rosa_laevigata	RLG00000022676
rosa_multiflora	Rmu_sc0031350.1_g000001
rosa_roxburghii	Rroxscaffold_6G00391150
rosa_rugosa	Rorug03G0264700
rosa_samantha	Rh3AG315100 Rh3AG315200 Rh3BG350400 Rh3CG347100 Rh3DG349700
rosa_wichuraiana	Rw3G027520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	86
AccB1I	GGYRCC	2 cut(s)	160, 717
AciI	CCGC	1 cut(s)	1003
AclWI	GGATC	2 cut(s)	1143, 1156
AcsI	RAATTY	6 cut(s)	25, 50, 902, 948, 983, 1069
AcuI	CTGAAG	1 cut(s)	242
AcyI	GRCGYC	1 cut(s)	83
AfaI	GTAC	6 cut(s)	130, 382, 680, 845, 1124, 1187
AfiI	CCNNNNNNNGG	4 cut(s)	53, 54, 654, 1009
AflII	CTTAAG	1 cut(s)	431
AhlI	ACTAGT	1 cut(s)	71
AjnI	CCWGG	6 cut(s)	234, 333, 476, 488, 720, 1059
AluBI	AGCT	4 cut(s)	418, 455, 869, 1027
AluI	AGCT	4 cut(s)	418, 455, 869, 1027
Alw21I	GWGCWC	1 cut(s)	92
Alw26I	GTCTC	4 cut(s)	143, 191, 813, 920
AlwI	GGATC	2 cut(s)	1143, 1156
Ama87I	CYCGRG	1 cut(s)	674
AoxI	GGCC	3 cut(s)	337, 514, 1101
ApaI	GGGCCC	1 cut(s)	341
ApeKI	GCWGC	3 cut(s)	320, 1094, 1118
ApoI	RAATTY	6 cut(s)	25, 50, 902, 948, 983, 1069
ArsI	GACNNNNNNTTYG	2 cut(s)	791, 823
AspLEI	GCGC	2 cut(s)	7, 769
AspS9I	GGNCC	2 cut(s)	337, 338
AsuHPI	GGTGA	3 cut(s)	637, 752, 1075
AvaI	CYCGRG	1 cut(s)	674
BaeGI	GKGCMC	1 cut(s)	341
BamHI	GGATCC	1 cut(s)	1148
BanI	GGYRCC	2 cut(s)	160, 717
BanII	GRGCYC	2 cut(s)	341, 1176
Bbv12I	GWGCWC	1 cut(s)	92
BbvI	GCAGC	3 cut(s)	307, 1081, 1105
BccI	CCATC	3 cut(s)	26, 149, 187
BceAI	ACGGC	1 cut(s)	944
BcgI	CGANNNNNNTGC	2 cut(s)	801, 835
BciT130I	CCWGG	6 cut(s)	236, 335, 478, 490, 722, 1061
BciVI	GTATCC	2 cut(s)	623, 1074
BclI	TGATCA	1 cut(s)	96
BcoDI	GTCTC	4 cut(s)	143, 191, 813, 920
BcuI	ACTAGT	1 cut(s)	71
BfaI	CTAG	1 cut(s)	72
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	431
BfuI	GTATCC	2 cut(s)	623, 1074
BglII	AGATCT	1 cut(s)	351
BisI	GCNGC	3 cut(s)	321, 1095, 1119
BlsI	GCNGC	3 cut(s)	322, 1096, 1120
BmcAI	AGTACT	1 cut(s)	130
Bme1390I	CCNGG	6 cut(s)	236, 335, 478, 490, 722, 1061
BmeT110I	CYCGRG	1 cut(s)	674
BmgT120I	GGNCC	2 cut(s)	337, 338
BmiI	GGNNCC	5 cut(s)	162, 339, 340, 719, 1150
BmrFI	CCNGG	6 cut(s)	236, 335, 478, 490, 722, 1061
BmrI	ACTGGG	1 cut(s)	769
BmsI	GCATC	2 cut(s)	667, 891
BmuI	ACTGGG	1 cut(s)	769
BplI	GAGNNNNNCTC	2 cut(s)	449, 481
BpmI	CTGGAG	2 cut(s)	317, 655
BpuEI	CTTGAG	1 cut(s)	647
BsaHI	GRCGYC	1 cut(s)	83
BsaI	GGTCTC	2 cut(s)	191, 813
BsaJI	CCNNGG	5 cut(s)	164, 334, 477, 558, 1059
BsaXI	ACNNNNNCTCC	2 cut(s)	140, 170
Bsc4I	CCNNNNNNNGG	4 cut(s)	53, 54, 654, 1009
Bse118I	RCCGGY	1 cut(s)	497
Bse1I	ACTGG	6 cut(s)	126, 446, 638, 682, 775, 794
Bse3DI	GCAATG	2 cut(s)	40, 1029
BseBI	CCWGG	6 cut(s)	236, 335, 478, 490, 722, 1061
BseDI	CCNNGG	5 cut(s)	164, 334, 477, 558, 1059
BseGI	GGATG	2 cut(s)	487, 730
BseLI	CCNNNNNNNGG	4 cut(s)	53, 54, 654, 1009
BseMI	GCAATG	2 cut(s)	40, 1029
BseMII	CTCAG	4 cut(s)	358, 378, 447, 609
BseNI	ACTGG	6 cut(s)	126, 446, 638, 682, 775, 794
BseSI	GKGCMC	1 cut(s)	341
BseXI	GCAGC	3 cut(s)	307, 1081, 1105
BseYI	CCCAGC	1 cut(s)	955
BsgI	GTGCAG	1 cut(s)	738
BshFI	GGCC	3 cut(s)	339, 516, 1103
BshNI	GGYRCC	2 cut(s)	160, 717
BsiHKAI	GWGCWC	1 cut(s)	92
BsiHKCI	CYCGRG	1 cut(s)	674
BsiSI	CCGG	1 cut(s)	498
BslI	CCNNNNNNNGG	4 cut(s)	53, 54, 654, 1009
BsmAI	GTCTC	4 cut(s)	143, 191, 813, 920
BsnI	GGCC	3 cut(s)	339, 516, 1103
Bso31I	GGTCTC	2 cut(s)	191, 813
BsoBI	CYCGRG	1 cut(s)	674
Bsp120I	GGGCCC	1 cut(s)	337
Bsp1286I	GDGCHC	3 cut(s)	92, 341, 1176
Bsp143I	GATC	3 cut(s)	96, 351, 1148
BspACI	CCGC	1 cut(s)	1003
BspANI	GGCC	3 cut(s)	339, 516, 1103
BspCNI	CTCAG	4 cut(s)	357, 379, 448, 610
BspHI	TCATGA	1 cut(s)	759
BspLI	GGNNCC	5 cut(s)	162, 339, 340, 719, 1150
BspPI	GGATC	2 cut(s)	1143, 1156
BspT107I	GGYRCC	2 cut(s)	160, 717
BspTI	CTTAAG	1 cut(s)	431
BspTNI	GGTCTC	2 cut(s)	191, 813
BsrDI	GCAATG	2 cut(s)	40, 1029
BsrFI	RCCGGY	1 cut(s)	497
BsrI	ACTGG	6 cut(s)	126, 446, 638, 682, 775, 794
BssAI	RCCGGY	1 cut(s)	497
BssECI	CCNNGG	5 cut(s)	164, 334, 477, 558, 1059
BssMI	GATC	3 cut(s)	96, 351, 1148
BssNI	GRCGYC	1 cut(s)	83
BssT1I	CCWWGG	2 cut(s)	164, 558
Bst2UI	CCWGG	6 cut(s)	236, 335, 478, 490, 722, 1061
Bst4CI	ACNGT	1 cut(s)	156
Bst6I	CTCTTC	1 cut(s)	1070
BstACI	GRCGYC	1 cut(s)	83
BstAFI	CTTAAG	1 cut(s)	431
BstC8I	GCNNGC	1 cut(s)	495
BstDEI	CTNAG	5 cut(s)	132, 344, 387, 456, 618
BstF5I	GGATG	2 cut(s)	487, 730
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 769
BstKTI	GATC	3 cut(s)	99, 354, 1151
BstMAI	GTCTC	4 cut(s)	143, 191, 813, 920
BstMBI	GATC	3 cut(s)	96, 351, 1148
BstMWI	GCNNNNNNNGC	3 cut(s)	75, 494, 1100
BstNI	CCWGG	6 cut(s)	236, 335, 478, 490, 722, 1061
BstNSI	RCATGY	2 cut(s)	497, 917
BstSCI	CCNGG	6 cut(s)	234, 333, 476, 488, 720, 1059
BstSLI	GKGCMC	1 cut(s)	341
BstV1I	GCAGC	3 cut(s)	307, 1081, 1105
BstX2I	RGATCY	2 cut(s)	351, 1148
BstXI	CCANNNNNNTGG	1 cut(s)	448
BstYI	RGATCY	2 cut(s)	351, 1148
BsuI	GTATCC	2 cut(s)	623, 1074
BsuRI	GGCC	3 cut(s)	339, 516, 1103
BtsCI	GGATG	2 cut(s)	487, 730
BtsIMutI	CAGTG	3 cut(s)	152, 453, 689
Cac8I	GCNNGC	1 cut(s)	495
CciI	TCATGA	1 cut(s)	759
CfoI	GCGC	2 cut(s)	7, 769
Cfr10I	RCCGGY	1 cut(s)	497
Cfr13I	GGNCC	2 cut(s)	337, 338
CsiI	ACCWGGT	1 cut(s)	234
Csp6I	GTAC	6 cut(s)	129, 381, 679, 844, 1123, 1186
CspCI	CAANNNNNGTGG	2 cut(s)	166, 201
CviAII	CATG	7 cut(s)	94, 472, 494, 737, 760, 914, 1006
CviQI	GTAC	6 cut(s)	129, 381, 679, 844, 1123, 1186
DdeI	CTNAG	5 cut(s)	132, 344, 387, 456, 618
DpnI	GATC	3 cut(s)	98, 353, 1150
DpnII	GATC	3 cut(s)	96, 351, 1148
Eam1104I	CTCTTC	1 cut(s)	1070
EarI	CTCTTC	1 cut(s)	1070
Eco130I	CCWWGG	2 cut(s)	164, 558
Eco147I	AGGCCT	1 cut(s)	516
Eco24I	GRGCYC	2 cut(s)	341, 1176
Eco31I	GGTCTC	2 cut(s)	191, 813
Eco57I	CTGAAG	1 cut(s)	242
Eco88I	CYCGRG	1 cut(s)	674
EcoO109I	RGGNCCY	2 cut(s)	337, 338
EcoRI	GAATTC	1 cut(s)	983
EcoRII	CCWGG	6 cut(s)	234, 333, 476, 488, 720, 1059
EcoT14I	CCWWGG	2 cut(s)	164, 558
EcoT22I	ATGCAT	1 cut(s)	915
EcoT38I	GRGCYC	2 cut(s)	341, 1176
ErhI	CCWWGG	2 cut(s)	164, 558
FaeI	CATG	7 cut(s)	97, 475, 497, 740, 763, 917, 1009
FalI	AAGNNNNNCTT	2 cut(s)	581, 613
FatI	CATG	7 cut(s)	93, 471, 493, 736, 759, 913, 1005
FbaI	TGATCA	1 cut(s)	96
Fnu4HI	GCNGC	3 cut(s)	321, 1095, 1119
FokI	GGATG	2 cut(s)	494, 737
FriOI	GRGCYC	2 cut(s)	341, 1176
Fsp4HI	GCNGC	3 cut(s)	321, 1095, 1119
FspBI	CTAG	1 cut(s)	72
GlaI	GCGC	2 cut(s)	6, 768
GluI	GCNGC	3 cut(s)	321, 1095, 1119
GsaI	CCCAGC	1 cut(s)	959
GsuI	CTGGAG	2 cut(s)	317, 655
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	3 cut(s)	339, 516, 1103
HapII	CCGG	1 cut(s)	498
HhaI	GCGC	2 cut(s)	7, 769
Hin1I	GRCGYC	1 cut(s)	83
Hin1II	CATG	7 cut(s)	97, 475, 497, 740, 763, 917, 1009
Hin6I	GCGC	2 cut(s)	5, 767
HinP1I	GCGC	2 cut(s)	5, 767
HinfI	GANTC	2 cut(s)	813, 1142
HpaII	CCGG	1 cut(s)	498
HphI	GGTGA	3 cut(s)	637, 752, 1075
Hpy166II	GTNNAC	2 cut(s)	114, 1186
Hpy188I	TCNGA	6 cut(s)	101, 347, 523, 555, 619, 1108
Hpy188III	TCNNGA	8 cut(s)	22, 260, 268, 505, 674, 760, 810, 1146
Hpy8I	GTNNAC	2 cut(s)	114, 1186
Hpy99I	CGWCG	4 cut(s)	88, 112, 1052, 1157
HpyAV	CCTTC	5 cut(s)	413, 420, 757, 787, 872
HpyCH4III	ACNGT	1 cut(s)	156
HpyCH4IV	ACGT	1 cut(s)	83
HpyCH4V	TGCA	4 cut(s)	755, 913, 1097, 1163
HpyF10VI	GCNNNNNNNGC	3 cut(s)	75, 494, 1100
HpyF3I	CTNAG	5 cut(s)	132, 344, 387, 456, 618
HpySE526I	ACGT	1 cut(s)	83
Hsp92I	GRCGYC	1 cut(s)	83
Hsp92II	CATG	7 cut(s)	97, 475, 497, 740, 763, 917, 1009
HspAI	GCGC	2 cut(s)	5, 767
Ksp22I	TGATCA	1 cut(s)	96
Kzo9I	GATC	3 cut(s)	96, 351, 1148
Lsp1109I	GCAGC	3 cut(s)	307, 1081, 1105
LweI	GCATC	2 cut(s)	667, 891
MabI	ACCWGGT	1 cut(s)	234
MaeI	CTAG	1 cut(s)	72
MaeII	ACGT	1 cut(s)	83
MaeIII	GTNAC	3 cut(s)	40, 740, 835
MalI	GATC	3 cut(s)	98, 353, 1150
MboI	GATC	3 cut(s)	96, 351, 1148
MboII	GAAGA	4 cut(s)	435, 589, 808, 1087
MfeI	CAATTG	1 cut(s)	1086
MflI	RGATCY	2 cut(s)	351, 1148
MhlI	GDGCHC	3 cut(s)	92, 341, 1176
MlyI	GAGTC	1 cut(s)	1151
MmeI	TCCRAC	1 cut(s)	501
MnlI	CCTC	6 cut(s)	241, 291, 353, 366, 1071, 1114
Mph1103I	ATGCAT	1 cut(s)	915
MseI	TTAA	3 cut(s)	432, 1017, 1127
MspCI	CTTAAG	1 cut(s)	431
MspI	CCGG	1 cut(s)	498
MspR9I	CCNGG	6 cut(s)	236, 335, 478, 490, 722, 1061
MunI	CAATTG	1 cut(s)	1086
MvaI	CCWGG	6 cut(s)	236, 335, 478, 490, 722, 1061
MwoI	GCNNNNNNNGC	3 cut(s)	75, 494, 1100
NdeII	GATC	3 cut(s)	96, 351, 1148
NlaIII	CATG	7 cut(s)	97, 475, 497, 740, 763, 917, 1009
NlaIV	GGNNCC	5 cut(s)	162, 339, 340, 719, 1150
NmuCI	GTSAC	2 cut(s)	740, 835
NsiI	ATGCAT	1 cut(s)	915
NspI	RCATGY	2 cut(s)	497, 917
PaeI	GCATGC	1 cut(s)	497
PagI	TCATGA	1 cut(s)	759
PceI	AGGCCT	1 cut(s)	516
PcsI	WCGNNNNNNNCGW	1 cut(s)	36
PfeI	GAWTC	1 cut(s)	813
PkrI	GCNGC	3 cut(s)	322, 1096, 1120
PleI	GAGTC	1 cut(s)	1150
PpsI	GAGTC	1 cut(s)	1150
Psp6I	CCWGG	6 cut(s)	234, 333, 476, 488, 720, 1059
PspFI	CCCAGC	1 cut(s)	955
PspGI	CCWGG	6 cut(s)	234, 333, 476, 488, 720, 1059
PspN4I	GGNNCC	5 cut(s)	162, 339, 340, 719, 1150
PspOMI	GGGCCC	1 cut(s)	337
PspPI	GGNCC	2 cut(s)	337, 338
PsuI	RGATCY	2 cut(s)	351, 1148
RsaI	GTAC	6 cut(s)	130, 382, 680, 845, 1124, 1187
RsaNI	GTAC	6 cut(s)	129, 381, 679, 844, 1123, 1186
SaqAI	TTAA	3 cut(s)	432, 1017, 1127
SatI	GCNGC	3 cut(s)	321, 1095, 1119
Sau3AI	GATC	3 cut(s)	96, 351, 1148
Sau96I	GGNCC	2 cut(s)	337, 338
ScaI	AGTACT	1 cut(s)	130
SchI	GAGTC	1 cut(s)	1151
ScrFI	CCNGG	6 cut(s)	236, 335, 478, 490, 722, 1061
SduI	GDGCHC	3 cut(s)	92, 341, 1176
SexAI	ACCWGGT	1 cut(s)	234
SfaNI	GCATC	2 cut(s)	667, 891
SmlI	CTYRAG	2 cut(s)	431, 662
SmoI	CTYRAG	2 cut(s)	431, 662
SpeI	ACTAGT	1 cut(s)	71
SphI	GCATGC	1 cut(s)	497
SseBI	AGGCCT	1 cut(s)	516
SsiI	CCGC	1 cut(s)	1003
SspMI	CTAG	1 cut(s)	72
StuI	AGGCCT	1 cut(s)	516
StyD4I	CCNGG	6 cut(s)	234, 333, 476, 488, 720, 1059
StyI	CCWWGG	2 cut(s)	164, 558
TaaI	ACNGT	1 cut(s)	156
TaiI	ACGT	1 cut(s)	86
TaqI	TCGA	5 cut(s)	86, 360, 811, 1155, 1170
TaqII	GACCGA	1 cut(s)	287
TatI	WGTACW	3 cut(s)	128, 380, 1185
TfiI	GAWTC	1 cut(s)	813
Tru1I	TTAA	3 cut(s)	432, 1017, 1127
Tru9I	TTAA	3 cut(s)	432, 1017, 1127
TscAI	CASTG	3 cut(s)	159, 453, 689
TseFI	GTSAC	2 cut(s)	740, 835
TseI	GCWGC	3 cut(s)	320, 1094, 1118
Tsp45I	GTSAC	2 cut(s)	740, 835
TspDTI	ATGAA	4 cut(s)	498, 583, 776, 891
TspGWI	ACGGA	2 cut(s)	877, 1141
TspRI	CASTG	3 cut(s)	159, 453, 689
Vha464I	CTTAAG	1 cut(s)	431
XapI	RAATTY	6 cut(s)	25, 50, 902, 948, 983, 1069
XceI	RCATGY	2 cut(s)	497, 917
XspI	CTAG	1 cut(s)	72
ZraI	GACGTC	1 cut(s)	84
ZrmI	AGTACT	1 cut(s)	130
Zsp2I	ATGCAT	1 cut(s)	915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.