RchiOBHm_Chr3g0496481

Ankyrin repeat and protein kinase domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013599
Physical Location & Seq
Forward (+)
47373171 .. 47374808
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1638 bp
ATGGACCGGCTCATAAGCTTGGAGCCATCAAATTTCGTTGCAATACGGATCGAACGAGGTCAGAAATGTTACGGCCTACTCACGTTGCGCAATGTCATGTACACCATGCCTGTGGCCTTCAGGCTTCAAGCCCTGGTCAAGAATCGCTACACGGTCAGACCTCAATCCGGGATCATATCTCCGCTCGAGAAGCTAACCATTGAGATCGTCTATCACCTTCCACCGGGATCTGGCCTCCCGGACTCGTTCCCTTATTGCCATGACTCCTTCCTTTTGCATAGCGTGGTCGTGCCCGGAGCAGCCATCAAAAACTCTTCATCGACTTTCGATGCGGTCCCCAGTGACTGGTTCACAACAAAGAAGAAGCAAGTGTTCATAGACAGCGGTGTGAAGATTATGTTCACTGGTTCACCTGTTTTGGCACAACTTGTGTCCGATGGTTTGATGGACGATATCAGAGAAGTGCTCGAGAAGAGCGACCCTTCATGGAAAGCAGCCGACTCTGTGGATTCGGATGGTCAATCTTTACTTCACTTGGCTATTACTCAAGGCCGACCCGACCTCGTCCAGCTGCTTCTCGAATTCAAGCCAGACGTGGAGGCTCAGAGCCGTTCGGGGTCGAGCCCGCTTGAGGCCGCGGCTGCTTCGGGTGAGGCTTTGATAGTCGAGCTTCTGTTGGCTCGTCGTGCAAGCACTGAAAGATCAGAGTCATCCACTTGGGGACCTATCCACCTGGCAGCCGGAGCTGGCCACGTCGAAGTTCTCAGGCTTCTTATAATTAAGGGGGCTAATGTTGATGCTCAAACAAAAGACGGAAACACCGCCTTACACCTAGCAGTGGAAGAGCGCAGAAGGGACTGTGCGAGGCTTTTGCTAGCAAGTGGCGCTAAGGCCGAAGTTCGTAATAGCAAGGAGGGTGACACCCCGCTGCACATAGCCGCAGGGTTGGGAGATGAGTACATGGTCAAGCTGTTGCTGCAAAAGGGTACTGCCAATAAAGATATCAGAAACTACGCCGGTAGAACGGCTTATGATGTTGCGGCAGAGAATGGTCACACCAAGCTCTTTGACTCCCTTCGGCTCGGAGATAGCTTGTGTATAGCGGCCAGGAAAGGAGAGGTGAGAACAATTCTGAGGCTGCTGGAGACCGGGGCCGCGATCAACGGAAGAGACCAACATGGGTGGACTGCTCTGCATAGGGCGTGCTTTAAAGGCAAAGTTGACGCCGTGCGATCGCTGCTGGAGAAGGGTGTGGATGTGGATGCTAAAGATGAGGATGGATACACGGCATTGCACTGCGCAGCGGAATCGGGGCATGCGGATGTGATAGATATGTTGGTGAAGAAAGGAGCTGATGCTGAAGCCAGGACTAATAAGGGTGTGACTGCGCTGCAGATTGCCGAGTCTTTGCACTATGCTGGGATTACAAGGATTCTTATGACACATGGAGGTGGTGCAATAAAAGATCAGAACATGGCACAGATTCTGTCTCACGCTTCAGTTCATTCGTTTGGGAAGAAATCCATGGGCGTTGAGGAAGAGATGATCAAGGGAGGGATGATGGTGAGGAAAAAATCGACTCGAGCTAGAGCTCTTCGTGGTAGCTTTGATCGTTCTGTGCCCTTGGCTGTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002262 GO:0002376 GO:0002520 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006887 GO:0006888 GO:0006996 GO:0007010 GO:0007163 GO:0007275 GO:0008092 GO:0008093 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010638 GO:0012505 GO:0014731 GO:0015672 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016528 GO:0016529 GO:0018130 GO:0019438 GO:0019899 GO:0030011 GO:0030016 GO:0030017 GO:0030018 GO:0030097 GO:0030099 GO:0030154 GO:0030218 GO:0030424 GO:0030507 GO:0030673 GO:0030674 GO:0030863 GO:0031252 GO:0031253 GO:0031256 GO:0031430 GO:0031672 GO:0031674 GO:0032501 GO:0032502 GO:0032589 GO:0032940 GO:0033013 GO:0033014 GO:0033036 GO:0033043 GO:0033267 GO:0034101 GO:0034613 GO:0034641 GO:0035088 GO:0035090 GO:0042383 GO:0042592 GO:0042995 GO:0043005 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043292 GO:0044237 GO:0044249 GO:0044271 GO:0044304 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044448 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045197 GO:0045199 GO:0045202 GO:0045211 GO:0046483 GO:0046903 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048731 GO:0048821 GO:0048856 GO:0048869 GO:0048872 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051117 GO:0051128 GO:0051130 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055072 GO:0055076 GO:0055080 GO:0060090 GO:0061245 GO:0061515 GO:0065007 GO:0065008 GO:0070727 GO:0071704 GO:0071840 GO:0071944 GO:0072657 GO:0072659 GO:0097060 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099512 GO:0099568 GO:0120025 GO:0120038 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990778
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 Motile_Sperm

Protein Analysis

545

Amino Acids

58.97

Weight (kDa)

8.37

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 5 - 107 1.9e-07 MSP (Major sperm protein) domain
Ank_2 PF12796 164 - 230 6.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 221 - 301 5.3e-14 Ankyrin repeats (3 copies)
Ank PF00023 242 - 270 3e-06 Ankyrin repeat
Ank_4 PF13637 243 - 292 2.7e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 263 - 313 3e-06 Ankyrin repeats (many copies)
Ank PF00023 271 - 302 7.6e-06 Ankyrin repeat
Ank_2 PF12796 299 - 359 7e-09 Ankyrin repeats (3 copies)
Ank PF00023 306 - 337 2.4e-07 Ankyrin repeat
Ank_2 PF12796 365 - 455 1.4e-20 Ankyrin repeats (3 copies)
Ank_4 PF13637 365 - 410 2.1e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 380 - 432 9.6e-09 Ankyrin repeats (many copies)
Ank PF00023 393 - 424 2.6e-07 Ankyrin repeat
Ank_4 PF13637 398 - 447 1.6e-14 Ankyrin repeats (many copies)
Ank_5 PF13857 413 - 467 3.1e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 423 - 483 3e-09 Ankyrin repeats (3 copies)
Ank PF00023 426 - 458 5.5e-08 Ankyrin repeat
Ank_3 PF13606 426 - 454 7.2e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
Acc16I TGCGCA 2 cut(s) 89, 1300
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 184
AccII CGCG 2 cut(s) 638, 1157
AclWI GGATC 3 cut(s) 56, 179, 235
AcoI YGGCCR 2 cut(s) 748, 1104
AcsI RAATTY 2 cut(s) 31, 581
AcuI CTGAAG 3 cut(s) 103, 1380, 1482
AcyI GRCGYC 1 cut(s) 1224
AfaI GTAC 3 cut(s) 101, 959, 988
AfiI CCNNNNNNNGG 6 cut(s) 167, 223, 230, 345, 631, 838
AgsI TTSAA 2 cut(s) 128, 586
AjiI CACGTC 2 cut(s) 595, 754
AjnI CCWGG 4 cut(s) 132, 732, 1106, 1364
Alw21I GWGCWC 2 cut(s) 468, 1594
Alw26I GTCTC 3 cut(s) 1139, 1164, 1494
AlwI GGATC 3 cut(s) 56, 179, 235
AlwNI CAGNNNCTG 2 cut(s) 345, 1486
Ama87I CYCGRG 3 cut(s) 185, 467, 1581
AoxI GGCC 9 cut(s) 73, 114, 232, 550, 633, 748, 891, 1104, 1152
ApoI RAATTY 2 cut(s) 31, 581
AsiSI GCGATCGC 1 cut(s) 1235
AspLEI GCGC 5 cut(s) 90, 849, 887, 1301, 1390
AspS9I GGNCC 4 cut(s) 4, 334, 722, 1152
AsuC2I CCSGG 5 cut(s) 169, 225, 239, 294, 1150
AsuHPI GGTGA 7 cut(s) 206, 402, 662, 929, 1132, 1351, 1576
AsuNHI GCTAGC 1 cut(s) 874
AvaI CYCGRG 3 cut(s) 185, 467, 1581
AvaII GGWCC 3 cut(s) 4, 334, 722
BaeGI GKGCMC 2 cut(s) 294, 1623
BalI TGGCCA 1 cut(s) 750
BanII GRGCYC 2 cut(s) 626, 1594
Bbv12I GWGCWC 2 cut(s) 468, 1594
BccI CCATC 7 cut(s) 34, 311, 431, 439, 509, 1271, 1555
BceAI ACGGC 5 cut(s) 88, 594, 1041, 1211, 1302
BcgI CGANNNNNNTGC 4 cut(s) 853, 887, 1290, 1324
BciT130I CCWGG 4 cut(s) 134, 734, 1108, 1366
BciVI GTATCC 1 cut(s) 1274
BclI TGATCA 1 cut(s) 1545
BcnI CCSGG 5 cut(s) 169, 225, 239, 294, 1150
BcoDI GTCTC 3 cut(s) 1139, 1164, 1494
BfaI CTAG 3 cut(s) 833, 875, 1587
BfmI CTRYAG 1 cut(s) 1391
BfoI RGCGCY 1 cut(s) 888
BfuI GTATCC 1 cut(s) 1274
Bme1390I CCNGG 9 cut(s) 134, 169, 225, 239, 294, 734, 1108, 1150, 1366
Bme18I GGWCC 3 cut(s) 4, 334, 722
BmeT110I CYCGRG 3 cut(s) 185, 467, 1581
BmgBI CACGTC 2 cut(s) 595, 754
BmgT120I GGNCC 4 cut(s) 4, 334, 722, 1152
BmiI GGNNCC 4 cut(s) 24, 336, 723, 1153
BmrFI CCNGG 9 cut(s) 134, 169, 225, 239, 294, 734, 1108, 1150, 1366
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 4 cut(s) 319, 787, 1252, 1345
BmtI GCTAGC 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 450, 482
BpmI CTGGAG 2 cut(s) 1163, 1262
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 531, 650
BpuMI CCSGG 5 cut(s) 169, 225, 239, 294, 1150
BsaHI GRCGYC 1 cut(s) 1224
BsaI GGTCTC 2 cut(s) 1139, 1164
BsaJI CCNNGG 5 cut(s) 132, 636, 1149, 1524, 1623
Bsc4I CCNNNNNNNGG 6 cut(s) 167, 223, 230, 345, 631, 838
Bse118I RCCGGY 2 cut(s) 6, 1016
Bse1I ACTGG 3 cut(s) 339, 350, 409
Bse3DI GCAATG 2 cut(s) 97, 1289
BseBI CCWGG 4 cut(s) 134, 734, 1108, 1366
BseDI CCNNGG 5 cut(s) 132, 636, 1149, 1524, 1623
BseGI GGATG 7 cut(s) 520, 710, 1261, 1267, 1282, 1327, 1563
BseLI CCNNNNNNNGG 6 cut(s) 167, 223, 230, 345, 631, 838
BseMI GCAATG 2 cut(s) 97, 1289
BseMII CTCAG 3 cut(s) 617, 778, 1124
BseNI ACTGG 3 cut(s) 339, 350, 409
BseSI GKGCMC 2 cut(s) 294, 1623
BseYI CCCAGC 1 cut(s) 1418
BsgI GTGCAG 1 cut(s) 914
Bsh1236I CGCG 2 cut(s) 638, 1157
Bsh1285I CGRYCG 1 cut(s) 1235
BshFI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
BsiEI CGRYCG 1 cut(s) 1235
BsiHKAI GWGCWC 2 cut(s) 468, 1594
BsiHKCI CYCGRG 3 cut(s) 185, 467, 1581
BsiSI CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1149
BslFI GGGAC 3 cut(s) 320, 735, 869
BslI CCNNNNNNNGG 6 cut(s) 167, 223, 230, 345, 631, 838
BsmAI GTCTC 3 cut(s) 1139, 1164, 1494
BsmFI GGGAC 3 cut(s) 320, 735, 869
BsnI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
Bso31I GGTCTC 2 cut(s) 1139, 1164
BsoBI CYCGRG 3 cut(s) 185, 467, 1581
Bsp1286I GDGCHC 5 cut(s) 294, 468, 626, 1594, 1623
Bsp1407I TGTACA 1 cut(s) 99
Bsp19I CCATGG 1 cut(s) 1524
BspANI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
BspCNI CTCAG 3 cut(s) 616, 777, 1125
BspFNI CGCG 2 cut(s) 638, 1157
BspLI GGNNCC 4 cut(s) 24, 336, 723, 1153
BspMAI CTGCAG 1 cut(s) 1395
BspOI GCTAGC 1 cut(s) 878
BspPI GGATC 3 cut(s) 56, 179, 235
BspQI GCTCTTC 3 cut(s) 467, 837, 1599
BspTNI GGTCTC 2 cut(s) 1139, 1164
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 97, 1289
BsrFI RCCGGY 2 cut(s) 6, 1016
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 3 cut(s) 339, 350, 409
BssAI RCCGGY 2 cut(s) 6, 1016
BssECI CCNNGG 5 cut(s) 132, 636, 1149, 1524, 1623
BssNI GRCGYC 1 cut(s) 1224
BssT1I CCWWGG 2 cut(s) 1524, 1623
Bst2UI CCWGG 4 cut(s) 134, 734, 1108, 1366
Bst4CI ACNGT 2 cut(s) 154, 860
Bst6I CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1599
BstACI GRCGYC 1 cut(s) 1224
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 6 cut(s) 626, 691, 748, 876, 1204, 1317
BstDEI CTNAG 4 cut(s) 603, 764, 888, 1133
BstDSI CCRYGG 2 cut(s) 636, 1524
BstF5I GGATG 7 cut(s) 520, 710, 1261, 1267, 1282, 1327, 1563
BstFNI CGCG 2 cut(s) 638, 1157
BstH2I RGCGCY 1 cut(s) 888
BstHHI GCGC 5 cut(s) 90, 849, 887, 1301, 1390
BstMAI GTCTC 3 cut(s) 1139, 1164, 1494
BstMCI CGRYCG 1 cut(s) 1235
BstMWI GCNNNNNNNGC 8 cut(s) 190, 641, 686, 743, 884, 976, 1212, 1237
BstNI CCWGG 4 cut(s) 134, 734, 1108, 1366
BstNSI RCATGY 1 cut(s) 1319
BstSCI CCNGG 9 cut(s) 132, 167, 223, 237, 292, 732, 1106, 1148, 1364
BstSFI CTRYAG 1 cut(s) 1391
BstSLI GKGCMC 2 cut(s) 294, 1623
BstUI CGCG 2 cut(s) 638, 1157
BstX2I RGATCY 1 cut(s) 227
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 1 cut(s) 227
BsuI GTATCC 1 cut(s) 1274
BsuRI GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
BtgI CCRYGG 2 cut(s) 636, 1524
BtrI CACGTC 2 cut(s) 595, 754
BtsCI GGATG 7 cut(s) 520, 710, 1261, 1267, 1282, 1327, 1563
BtsI GCAGTG 2 cut(s) 843, 1294
BtsIMutI CAGTG 5 cut(s) 346, 402, 693, 843, 1294
Cac8I GCNNGC 6 cut(s) 626, 691, 748, 876, 1204, 1317
CaiI CAGNNNCTG 2 cut(s) 345, 1486
CfoI GCGC 5 cut(s) 90, 849, 887, 1301, 1390
Cfr10I RCCGGY 2 cut(s) 6, 1016
Cfr13I GGNCC 4 cut(s) 4, 334, 722, 1152
Cfr42I CCGCGG 1 cut(s) 639
CseI GACGC 1 cut(s) 1232
Csp6I GTAC 3 cut(s) 100, 958, 987
CspCI CAANNNNNGTGG 2 cut(s) 1163, 1198
CviQI GTAC 3 cut(s) 100, 958, 987
DdeI CTNAG 4 cut(s) 603, 764, 888, 1133
DraI TTTAAA 1 cut(s) 1210
EaeI YGGCCR 2 cut(s) 748, 1104
Eam1104I CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1599
EarI CTCTTC 6 cut(s) 319, 467, 837, 1162, 1533, 1599
Ecl136II GAGCTC 1 cut(s) 1592
Eco130I CCWWGG 2 cut(s) 1524, 1623
Eco24I GRGCYC 2 cut(s) 626, 1594
Eco31I GGTCTC 2 cut(s) 1139, 1164
Eco32I GATATC 2 cut(s) 454, 1003
Eco47I GGWCC 3 cut(s) 4, 334, 722
Eco53kI GAGCTC 1 cut(s) 1592
Eco57I CTGAAG 3 cut(s) 103, 1380, 1482
Eco88I CYCGRG 3 cut(s) 185, 467, 1581
EcoICRI GAGCTC 1 cut(s) 1592
EcoO109I RGGNCCY 1 cut(s) 722
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 4 cut(s) 132, 732, 1106, 1364
EcoRV GATATC 2 cut(s) 454, 1003
EcoT14I CCWWGG 2 cut(s) 1524, 1623
EcoT38I GRGCYC 2 cut(s) 626, 1594
ErhI CCWWGG 2 cut(s) 1524, 1623
FaqI GGGAC 3 cut(s) 320, 735, 869
FauI CCCGC 2 cut(s) 633, 933
FbaI TGATCA 1 cut(s) 1545
FokI GGATG 7 cut(s) 527, 697, 1268, 1274, 1289, 1334, 1570
FriOI GRGCYC 2 cut(s) 626, 1594
FspBI CTAG 3 cut(s) 833, 875, 1587
FspI TGCGCA 2 cut(s) 89, 1300
GlaI GCGC 5 cut(s) 89, 848, 886, 1300, 1389
GsaI CCCAGC 1 cut(s) 1422
GsuI CTGGAG 2 cut(s) 1163, 1262
HaeII RGCGCY 1 cut(s) 888
HaeIII GGCC 9 cut(s) 75, 116, 234, 552, 635, 750, 893, 1106, 1154
HapII CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1149
HgaI GACGC 1 cut(s) 1232
HhaI GCGC 5 cut(s) 90, 849, 887, 1301, 1390
Hin1I GRCGYC 1 cut(s) 1224
Hin6I GCGC 5 cut(s) 88, 847, 885, 1299, 1388
HinP1I GCGC 5 cut(s) 88, 847, 885, 1299, 1388
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 16
HpaII CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1149
HphI GGTGA 7 cut(s) 206, 402, 662, 929, 1132, 1351, 1576
Hpy166II GTNNAC 6 cut(s) 102, 351, 402, 410, 1185, 1222
Hpy188III TCNNGA 4 cut(s) 139, 187, 469, 578
Hpy8I GTNNAC 6 cut(s) 102, 351, 402, 410, 1185, 1222
Hpy99I CGWCG 2 cut(s) 687, 758
HpyAV CCTTC 7 cut(s) 127, 227, 277, 492, 846, 1085, 1240
HpyCH4III ACNGT 2 cut(s) 154, 860
HpyCH4IV ACGT 3 cut(s) 83, 594, 753
HpyF10VI GCNNNNNNNGC 8 cut(s) 190, 641, 686, 743, 884, 976, 1212, 1237
HpyF3I CTNAG 4 cut(s) 603, 764, 888, 1133
HpySE526I ACGT 3 cut(s) 83, 594, 753
Hsp92I GRCGYC 1 cut(s) 1224
HspAI GCGC 5 cut(s) 88, 847, 885, 1299, 1388
Ksp22I TGATCA 1 cut(s) 1545
KspI CCGCGG 1 cut(s) 639
LguI GCTCTTC 3 cut(s) 467, 837, 1599
LmnI GCTCC 4 cut(s) 22, 296, 743, 1349
LweI GCATC 4 cut(s) 319, 787, 1252, 1345
MaeI CTAG 3 cut(s) 833, 875, 1587
MaeII ACGT 3 cut(s) 83, 594, 753
MaeIII GTNAC 5 cut(s) 68, 341, 917, 1052, 1381
MbiI CCGCTC 1 cut(s) 184
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 5 cut(s) 294, 468, 626, 1594, 1623
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 4 cut(s) 31, 581, 777, 1128
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 7 cut(s) 236, 257, 494, 716, 1064, 1412, 1573
Mox20I TGGCCA 1 cut(s) 750
MscI TGGCCA 1 cut(s) 750
MseI TTAA 3 cut(s) 780, 1209, 1636
MslI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 5 cut(s) 384, 571, 638, 928, 1304
MspI CCGG 8 cut(s) 7, 168, 224, 239, 294, 741, 1017, 1149
MspR9I CCNGG 9 cut(s) 134, 169, 225, 239, 294, 734, 1108, 1150, 1366
MvaI CCWGG 4 cut(s) 134, 734, 1108, 1366
MvnI CGCG 2 cut(s) 638, 1157
MwoI GCNNNNNNNGC 8 cut(s) 190, 641, 686, 743, 884, 976, 1212, 1237
NciI CCSGG 5 cut(s) 169, 225, 239, 294, 1150
NcoI CCATGG 1 cut(s) 1524
NheI GCTAGC 1 cut(s) 874
NlaIV GGNNCC 4 cut(s) 24, 336, 723, 1153
NmeAIII GCCGAG 1 cut(s) 1426
NmuCI GTSAC 4 cut(s) 341, 917, 1052, 1381
NsbI TGCGCA 2 cut(s) 89, 1300
NspI RCATGY 1 cut(s) 1319
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 3 cut(s) 185, 467, 1581
PciSI GCTCTTC 3 cut(s) 467, 837, 1599
PcsI WCGNNNNNNNCGW 2 cut(s) 52, 474
PfeI GAWTC 5 cut(s) 142, 509, 1307, 1432, 1483
PflFI GACNNNGTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 167, 237
Ple19I CGATCG 1 cut(s) 1235
PleI GAGTC 7 cut(s) 236, 257, 494, 715, 1064, 1411, 1573
PpsI GAGTC 7 cut(s) 236, 257, 494, 715, 1064, 1411, 1573
PpuMI RGGWCCY 1 cut(s) 722
PsiI TTATAA 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 1594
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 4 cut(s) 132, 732, 1106, 1364
PspFI CCCAGC 1 cut(s) 1418
PspGI CCWGG 4 cut(s) 132, 732, 1106, 1364
PspN4I GGNNCC 4 cut(s) 24, 336, 723, 1153
PspPI GGNCC 4 cut(s) 4, 334, 722, 1152
PspPPI RGGWCCY 1 cut(s) 722
PspXI VCTCGAGB 1 cut(s) 1581
PstI CTGCAG 1 cut(s) 1395
PstNI CAGNNNCTG 2 cut(s) 345, 1486
PsuI RGATCY 1 cut(s) 227
PsyI GACNNNGTC 1 cut(s) 563
PvuI CGATCG 1 cut(s) 1235
PvuII CAGCTG 1 cut(s) 571
RgaI GCGATCGC 1 cut(s) 1235
RsaI GTAC 3 cut(s) 101, 959, 988
RsaNI GTAC 3 cut(s) 100, 958, 987
RseI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SacI GAGCTC 1 cut(s) 1594
SacII CCGCGG 1 cut(s) 639
SapI GCTCTTC 3 cut(s) 467, 837, 1599
SaqAI TTAA 3 cut(s) 780, 1209, 1636
Sau96I GGNCC 4 cut(s) 4, 334, 722, 1152
SchI GAGTC 7 cut(s) 236, 257, 494, 716, 1064, 1412, 1573
ScrFI CCNGG 9 cut(s) 134, 169, 225, 239, 294, 734, 1108, 1150, 1366
SduI GDGCHC 5 cut(s) 294, 468, 626, 1594, 1623
SfaAI GCGATCGC 1 cut(s) 1235
SfaNI GCATC 4 cut(s) 319, 787, 1252, 1345
SfcI CTRYAG 1 cut(s) 1391
Sfr274I CTCGAG 3 cut(s) 185, 467, 1581
Sfr303I CCGCGG 1 cut(s) 639
SgfI GCGATCGC 1 cut(s) 1235
SgrBI CCGCGG 1 cut(s) 639
SinI GGWCC 3 cut(s) 4, 334, 722
SlaI CTCGAG 3 cut(s) 185, 467, 1581
SmiMI CAYNNNNRTG 3 cut(s) 110, 1320, 1449
SmlI CTYRAG 5 cut(s) 185, 467, 546, 629, 1581
SmoI CTYRAG 5 cut(s) 185, 467, 546, 629, 1581
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 4 cut(s) 31, 581, 777, 1128
SspMI CTAG 3 cut(s) 833, 875, 1587
SstI GAGCTC 1 cut(s) 1594
StyD4I CCNGG 9 cut(s) 132, 167, 223, 237, 292, 732, 1106, 1148, 1364
StyI CCWWGG 2 cut(s) 1524, 1623
TaaI ACNGT 2 cut(s) 154, 860
TaiI ACGT 3 cut(s) 86, 597, 756
TasI AATT 4 cut(s) 31, 581, 777, 1128
TatI WGTACW 2 cut(s) 99, 957
TauI GCSGC 6 cut(s) 638, 641, 941, 1043, 1106, 1157
TfiI GAWTC 5 cut(s) 142, 509, 1307, 1432, 1483
Tru1I TTAA 3 cut(s) 780, 1209, 1636
Tru9I TTAA 3 cut(s) 780, 1209, 1636
TscAI CASTG 5 cut(s) 346, 409, 700, 843, 1301
TseFI GTSAC 4 cut(s) 341, 917, 1052, 1381
Tsp45I GTSAC 4 cut(s) 341, 917, 1052, 1381
TspDTI ATGAA 4 cut(s) 306, 364, 474, 1493
TspGWI ACGGA 3 cut(s) 61, 828, 1179
TspRI CASTG 5 cut(s) 346, 409, 700, 843, 1301
Tth111I GACNNNGTC 1 cut(s) 563
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 4, 334, 722
XapI RAATTY 2 cut(s) 31, 581
XceI RCATGY 1 cut(s) 1319
XhoI CTCGAG 3 cut(s) 185, 467, 1581
XspI CTAG 3 cut(s) 833, 875, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.