RchiOBHm_Chr2g0112781

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0013513
Physical Location & Seq
Forward (+)
24848793 .. 24849831
1039 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 396 bp
ATGCATTTCTCATCAGTGAATTCTTTTTTCACCATCCCCATGAATTCTTCAATAAATCTTCGGTTTTTCACCGCAACCAGAGCCATTATTGTTCTTCTTCTTCTTGTTCTCTCTCCAGCCTCTTGTTTTATTTCTCTTCGGGGACTATTGTTTGCTGAGAAAGCCAGATTGGGCTCAACACCACCGAGCTGCCACAACAAGTGCAACCAGTGCCACCCTTGCATGGCGGTCCAAGTCCCCACCATGCCCAGCCGCGACCGGGTCCGACCCGTGGGGAAGACCCGACCCGCTAAGCCCATGGTGCTCTTTGACCCGTCTCATCTGGATAACAGGTACTCCAATTACAAGCCACTGGGTTGGAAATGCCATTGTGGTGACCACTTCTTCAATCCCTAG

Protein Analysis

131

Amino Acids

14.75

Weight (kDa)

9.77

Isoelectric Point (pI)

53.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 56 - 131 3.6e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013513)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10310 AT5G10310
fragaria_vesca FvH4_1g20560
malus_domestica MD01G1033300.v1.1 MD15G1322000.v1.1
prunus_persica Prupe.6G190800_v2.0.a1
pyrus_communis pycom01g06080
rosa_chinensis RchiOBHm_Chr2g0112781
rosa_laevigata RLG00000018009
rosa_multiflora Rmu_co8293215.1_g000001 Rmu_sc0001708.1_g000028 Rmu_sc0002351.1_g000005
rosa_roxburghii Rroxscaffold_2G00131150
rosa_rugosa Rorug02G0182700
rosa_samantha Rh2AG237200 Rh2BG250000
rosa_wichuraiana Rw2G018350 Rw2G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 4 cut(s) 72, 227, 253, 288
AcsI RAATTY 2 cut(s) 19, 43
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 3 cut(s) 223, 259, 271
AgsI TTSAA 2 cut(s) 51, 388
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 1 cut(s) 189
AluI AGCT 1 cut(s) 189
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 321
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 2 cut(s) 19, 43
AspS9I GGNCC 2 cut(s) 229, 262
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 3 cut(s) 22, 61, 386
AvaII GGWCC 2 cut(s) 229, 262
BanII GRGCYC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 176
BccI CCATC 1 cut(s) 41
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 1 cut(s) 394
BisI GCNGC 2 cut(s) 190, 253
BlpI GCTNAGC 1 cut(s) 291
BlsI GCNGC 2 cut(s) 191, 254
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 2 cut(s) 229, 262
BmgT120I GGNCC 2 cut(s) 229, 262
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 260
BmrI ACTGGG 1 cut(s) 362
BmuI ACTGGG 1 cut(s) 362
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 99
Bpu1102I GCTNAGC 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 270, 297
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 259, 271
Bse1I ACTGG 2 cut(s) 208, 357
BseDI CCNNGG 2 cut(s) 270, 297
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 3 cut(s) 223, 259, 271
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 2 cut(s) 208, 357
BseXI GCAGC 1 cut(s) 176
BseYI CCCAGC 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 259
BsiEI CGRYCG 1 cut(s) 259
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 1 cut(s) 259
BslFI GGGAC 2 cut(s) 156, 221
BslI CCNNNNNNNGG 3 cut(s) 223, 259, 271
BsmAI GTCTC 1 cut(s) 321
BsmBI CGTCTC 1 cut(s) 321
BsmFI GGGAC 2 cut(s) 156, 221
Bsp1286I GDGCHC 2 cut(s) 176, 306
Bsp1720I GCTNAGC 1 cut(s) 291
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 4 cut(s) 72, 227, 253, 288
BspCNI CTCAG 1 cut(s) 148
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 263
BsrI ACTGG 2 cut(s) 208, 357
BssECI CCNNGG 2 cut(s) 270, 297
BssT1I CCWWGG 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 156, 291
BstDSI CCRYGG 2 cut(s) 270, 297
BstEII GGTNACC 1 cut(s) 374
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 1 cut(s) 255
BstMAI GTCTC 1 cut(s) 321
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 5 cut(s) 80, 161, 210, 219, 301
BstPI GGTNACC 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 258
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 284
BstXI CCANNNNNNTGG 1 cut(s) 357
BtgI CCRYGG 2 cut(s) 270, 297
BtsCI GGATG 1 cut(s) 33
BtsIMutI CAGTG 3 cut(s) 21, 215, 350
Cfr13I GGNCC 2 cut(s) 229, 262
Csp6I GTAC 1 cut(s) 334
CviAII CATG 4 cut(s) 40, 223, 244, 298
CviJI RGCY 8 cut(s) 83, 119, 164, 174, 189, 252, 295, 349
CviKI_1 RGCY 8 cut(s) 83, 119, 164, 174, 189, 252, 295, 349
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 156, 291
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 2 cut(s) 229, 262
Eco91I GGTNACC 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 374
EcoRI GAATTC 2 cut(s) 19, 43
EcoT14I CCWWGG 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 297
Esp3I CGTCTC 1 cut(s) 321
FaeI CATG 4 cut(s) 43, 226, 247, 301
FaiI YATR 4 cut(s) 41, 224, 245, 299
FaqI GGGAC 2 cut(s) 156, 221
FatI CATG 4 cut(s) 39, 222, 243, 297
FauI CCCGC 1 cut(s) 295
Fnu4HI GCNGC 2 cut(s) 190, 253
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 190, 253
FspBI CTAG 1 cut(s) 394
GluI GCNGC 2 cut(s) 190, 253
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 99
HapII CCGG 1 cut(s) 259
Hin1II CATG 4 cut(s) 43, 226, 247, 301
HpaII CCGG 1 cut(s) 259
HphI GGTGA 3 cut(s) 22, 61, 386
Hpy188I TCNGA 1 cut(s) 266
Hpy188III TCNNGA 1 cut(s) 323
HpyCH4V TGCA 3 cut(s) 4, 204, 222
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 161, 210, 219, 301
HpyF3I CTNAG 2 cut(s) 156, 291
Hsp92II CATG 4 cut(s) 43, 226, 247, 301
LpnPI CCDG 9 cut(s) 91, 129, 178, 221, 262, 272, 308, 316, 338
Lsp1109I GCAGC 1 cut(s) 176
MaeI CTAG 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 374
MboII GAAGA 8 cut(s) 39, 50, 86, 89, 92, 128, 289, 376
MhlI GDGCHC 2 cut(s) 176, 306
MluCI AATT 3 cut(s) 19, 43, 340
MmeI TCCRAC 2 cut(s) 289, 338
MnlI CCTC 1 cut(s) 130
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 2 cut(s) 38, 372
MspI CCGG 1 cut(s) 259
MspR9I CCNGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 80, 161, 210, 219, 301
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 297
NlaIII CATG 4 cut(s) 43, 226, 247, 301
NlaIV GGNNCC 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 6
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 2 cut(s) 191, 254
PspEI GGTNACC 1 cut(s) 374
PspFI CCCAGC 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 2 cut(s) 229, 262
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 2 cut(s) 38, 372
SatI GCNGC 2 cut(s) 190, 253
Sau96I GGNCC 2 cut(s) 229, 262
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 2 cut(s) 176, 306
SetI ASST 2 cut(s) 191, 335
SinI GGWCC 2 cut(s) 229, 262
SmiMI CAYNNNNRTG 2 cut(s) 38, 372
Sse9I AATT 3 cut(s) 19, 43, 340
SsiI CCGC 4 cut(s) 72, 227, 253, 288
SspMI CTAG 1 cut(s) 394
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 1 cut(s) 297
TasI AATT 3 cut(s) 19, 43, 340
TauI GCSGC 1 cut(s) 255
TscAI CASTG 3 cut(s) 21, 215, 357
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 189
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 56
TspRI CASTG 3 cut(s) 21, 215, 357
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 229, 262
XapI RAATTY 2 cut(s) 19, 43
XspI CTAG 1 cut(s) 394
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.