RchiOBHm_Chr2g0117011

Belongs to the Nudix hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008057
Physical Location & Seq
Reverse (-)
29335999 .. 29338158
2160 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGGATATATGCCGATTTGCCTTGCATAGTTACCCGCTTTTTCTTCCCTCTTTATCAGTTCTCATCAAGCCTACAGTTGTATTGCCATCGTCCTCAAGCTGCCGTCCTAAACTAGCTAAGTTCGCACAGCTGTCAATCATTTACTCGAAACCAACTATTTATACTAAAGTGCGCTGTGCCTCCTCCTATTCTTCATCAGCATCATCGTCAATGGAAGCCCCTCCCGAAGGTTACAGAAGGAATGTTGGTATCTGCCTCATCAATGATTCAAAGAAGATTTTTGCTGCTTCAAGGTTAGATGTACCTGATTCTTGGCAAATGCCGCAAGGTGGCATTGATGAGGGTGAAGATCCAAGAACTGCAGCCATCAGGGAATTAAGAGAAGAGACAGGAGTTCAGTCGGCAGAAATTCTTGCAGAGACACCTAGTTGGTTAACCTATGATTTCCCACCAGAAGTTAGGGCAAAACTTCAGAAACAGTGGGGATCAGATTGGAAGGGCCAAGCACAGAAGTGGTTTCTTTTTCGGTTCACTGGGAAGGATGAAGAAGTCAATCTTCTAGGTGATGGCAGTGAAAAACCTGAGTTTGGCGAGTGGTCATGGATAACACCAGAACAAGTAGTTGATCTTGCTGTTGATTTTAAGAAGCCTGTTTACAAAGAAGTTCTTGCAGTTTTCGCACCCTATTTTCAATAA

Protein Analysis

231

Amino Acids

26.03

Weight (kDa)

5.63

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 78 - 220 2e-24 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 35, 323
AclWI GGATC 2 cut(s) 344, 493
AcsI RAATTY 1 cut(s) 408
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 1 cut(s) 303
AfiI CCNNNNNNNGG 3 cut(s) 227, 329, 587
AgsI TTSAA 3 cut(s) 270, 291, 692
AleI CACNNNNGTG 1 cut(s) 511
AluBI AGCT 3 cut(s) 99, 116, 130
AluI AGCT 3 cut(s) 99, 116, 130
Alw26I GTCTC 2 cut(s) 380, 413
AlwI GGATC 2 cut(s) 344, 493
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 3 cut(s) 99, 284, 362
ApoI RAATTY 1 cut(s) 408
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 499
AsuHPI GGTGA 2 cut(s) 356, 575
BarI GAAGNNNNNNTAC 2 cut(s) 638, 670
BbvI GCAGC 3 cut(s) 86, 271, 374
BccI CCATC 3 cut(s) 94, 374, 560
BceAI ACGGC 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 380, 413
BfaI CTAG 3 cut(s) 113, 426, 560
BfmI CTRYAG 2 cut(s) 72, 360
BisI GCNGC 4 cut(s) 100, 285, 323, 363
BlsI GCNGC 4 cut(s) 101, 286, 324, 364
BmgT120I GGNCC 1 cut(s) 499
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 1 cut(s) 209
BmuI ACTGGG 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 79
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 329, 587
Bse1I ACTGG 1 cut(s) 538
BseGI GGATG 1 cut(s) 547
BseLI CCNNNNNNNGG 3 cut(s) 227, 329, 587
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 86, 271, 374
BshFI GGCC 1 cut(s) 501
BslI CCNNNNNNNGG 3 cut(s) 227, 329, 587
BsmAI GTCTC 2 cut(s) 380, 413
BsnI GGCC 1 cut(s) 501
Bsp143I GATC 3 cut(s) 349, 485, 625
BspACI CCGC 2 cut(s) 35, 323
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 1 cut(s) 574
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 2 cut(s) 344, 493
BsrI ACTGG 1 cut(s) 538
BssMI GATC 3 cut(s) 349, 485, 625
Bst4CI ACNGT 2 cut(s) 76, 480
Bst6I CTCTTC 1 cut(s) 378
BstDEI CTNAG 2 cut(s) 117, 582
BstENI CCTNNNNNAGG 1 cut(s) 225
BstF5I GGATG 1 cut(s) 547
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 352, 488, 628
BstMAI GTCTC 2 cut(s) 380, 413
BstMBI GATC 3 cut(s) 349, 485, 625
BstMWI GCNNNNNNNGC 3 cut(s) 122, 322, 677
BstSFI CTRYAG 2 cut(s) 72, 360
BstV1I GCAGC 3 cut(s) 86, 271, 374
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 1 cut(s) 501
BtsCI GGATG 1 cut(s) 547
BtsI GCAGTG 1 cut(s) 577
BtsIMutI CAGTG 3 cut(s) 485, 531, 577
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 499
Csp6I GTAC 1 cut(s) 302
CviAII CATG 1 cut(s) 600
CviJI RGCY 8 cut(s) 70, 99, 116, 130, 218, 365, 501, 649
CviKI_1 RGCY 8 cut(s) 70, 99, 116, 130, 218, 365, 501, 649
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 2 cut(s) 117, 582
DpnI GATC 3 cut(s) 351, 487, 627
DpnII GATC 3 cut(s) 349, 485, 625
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 455
EcoNI CCTNNNNNAGG 1 cut(s) 225
FaeI CATG 1 cut(s) 603
FaiI YATR 6 cut(s) 8, 10, 27, 162, 441, 601
FalI AAGNNNNNCTT 4 cut(s) 540, 572, 651, 683
FatI CATG 1 cut(s) 599
FauI CCCGC 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 100, 285, 323, 363
FokI GGATG 1 cut(s) 554
Fsp4HI GCNGC 4 cut(s) 100, 285, 323, 363
FspBI CTAG 3 cut(s) 113, 426, 560
GlaI GCGC 1 cut(s) 173
GluI GCNGC 4 cut(s) 100, 285, 323, 363
HaeIII GGCC 1 cut(s) 501
HhaI GCGC 1 cut(s) 174
Hin1II CATG 1 cut(s) 603
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 2 cut(s) 266, 308
HpaI GTTAAC 1 cut(s) 435
HphI GGTGA 2 cut(s) 356, 575
Hpy166II GTNNAC 3 cut(s) 435, 531, 655
Hpy188I TCNGA 2 cut(s) 474, 490
Hpy188III TCNNGA 1 cut(s) 224
Hpy8I GTNNAC 3 cut(s) 435, 531, 655
HpyAV CCTTC 4 cut(s) 221, 231, 490, 532
HpyCH4III ACNGT 2 cut(s) 76, 480
HpyCH4V TGCA 4 cut(s) 25, 362, 416, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 322, 677
HpyF3I CTNAG 2 cut(s) 117, 582
Hsp92II CATG 1 cut(s) 603
HspAI GCGC 1 cut(s) 172
KspAI GTTAAC 1 cut(s) 435
Kzo9I GATC 3 cut(s) 349, 485, 625
LpnPI CCDG 8 cut(s) 318, 355, 375, 465, 519, 594, 624, 663
Lsp1109I GCAGC 3 cut(s) 86, 271, 374
LweI GCATC 1 cut(s) 209
MaeI CTAG 3 cut(s) 113, 426, 560
MaeIII GTNAC 2 cut(s) 29, 230
MalI GATC 3 cut(s) 351, 487, 627
MboI GATC 3 cut(s) 349, 485, 625
MboII GAAGA 7 cut(s) 35, 183, 286, 359, 395, 548, 557
MflI RGATCY 1 cut(s) 349
MluCI AATT 2 cut(s) 374, 408
MnlI CCTC 7 cut(s) 58, 103, 190, 193, 231, 266, 334
MseI TTAA 3 cut(s) 377, 434, 642
MslI CAYNNNNRTG 1 cut(s) 511
MspA1I CMGCKG 1 cut(s) 130
MwoI GCNNNNNNNGC 3 cut(s) 122, 322, 677
NdeII GATC 3 cut(s) 349, 485, 625
NlaIII CATG 1 cut(s) 603
OliI CACNNNNGTG 1 cut(s) 511
PfeI GAWTC 2 cut(s) 266, 308
PkrI GCNGC 4 cut(s) 101, 286, 324, 364
PspPI GGNCC 1 cut(s) 499
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 130
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 511
SaqAI TTAA 3 cut(s) 377, 434, 642
SatI GCNGC 4 cut(s) 100, 285, 323, 363
Sau3AI GATC 3 cut(s) 349, 485, 625
Sau96I GGNCC 1 cut(s) 499
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 2 cut(s) 72, 360
SmiMI CAYNNNNRTG 1 cut(s) 511
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 2 cut(s) 374, 408
SsiI CCGC 2 cut(s) 35, 323
SspMI CTAG 3 cut(s) 113, 426, 560
TaaI ACNGT 2 cut(s) 76, 480
TaqI TCGA 1 cut(s) 146
TasI AATT 2 cut(s) 374, 408
TauI GCSGC 1 cut(s) 325
TfiI GAWTC 2 cut(s) 266, 308
Tru1I TTAA 3 cut(s) 377, 434, 642
Tru9I TTAA 3 cut(s) 377, 434, 642
TscAI CASTG 3 cut(s) 485, 538, 577
TseI GCWGC 3 cut(s) 99, 284, 362
TspDTI ATGAA 2 cut(s) 183, 558
TspRI CASTG 3 cut(s) 485, 538, 577
XagI CCTNNNNNAGG 1 cut(s) 225
XapI RAATTY 1 cut(s) 408
XspI CTAG 3 cut(s) 113, 426, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.