RchiOBHm_Chr2g0118861

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015157
Physical Location & Seq
Reverse (-)
31077975 .. 31078688
714 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGCCTGCTTCTGCTTCTGCTTCTCACCAACTTCACGATCTTCTTGGGGACTTCTACTCCAGGAGACTACTCCTCCACAACCCACTTTACCCTCCACCCATCTCTCCATCTTCAACCTCGCACGACACATCTTCGTCCTCTCCTGATCAGTACCCCGGAGACAACAGCTTCGATGCTAATGTGGTCATGGTGCTCTCTGTCCTCCTCTGCGCCCTAATTTGCTCTTTGGGGCTCAACTCCATCATAAAGTGCGCTCTGAGGTGCTCGAGCTTCGTGTCCTCGGAGTCTTCTTCTTCCTCAAGAGACAACTCGGCGAGGCTGGCCAACACCGGAGTCAAGAAGAAAGCTCTCAAGACTTTTACGACGGTGAATTACTCAGCTGAGCTTAAGCTTCCCGGGTTGGACAGTGAGTGCATAATTTGTTTGTCGGAGTTTACAACCGGCGAGCGCGTGCGGCTACTTCCCAAGTGTAACCATGGATTCCATGTGAGGTGCATCGATAAGTGGCTGAGCTCTCACTCTTCGTGCCCTAAATGCCGGCACAGCTTGATCGAGACGTGTCAGAAGATCGTTGGCTTCAGCCAGGCAGATAGCTCGTCGGAGACAACTCGTGCTCTTCCTGTGCAGGAAACTACAGTAAGCATTGCAGCGCTTGAACCTGAAGGGTTTATTCGTAATTATAGGGGAATTATTAGCCAAGCTAGCTAG

Protein Analysis

235

Amino Acids

25.47

Weight (kDa)

8.01

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 135 - 180 3.7e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 137 - 180 2.5e-14 Ring finger domain
zf-RING_11 PF17123 137 - 166 2e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 137 - 179 1.4e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 138 - 179 3.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49200 AT1G49210 AT1G49220 AT1G49230 AT3G18773
fragaria_vesca FvH4_1g23480
prunus_persica Prupe.6G163700_v2.0.a1
pyrus_communis pycom01g02700
rosa_chinensis RchiOBHm_Chr2g0118861
rosa_laevigata RLG00000018427
rosa_roxburghii Rroxscaffold_2G00124640
rosa_rugosa Rorug02G0219400
rosa_samantha Rh2AG276400 Rh2DG283300 Rh2DG302600
rosa_wichuraiana Rw2G022000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 321
AcuI CTGAAG 2 cut(s) 562, 681
AfaI GTAC 1 cut(s) 152
AfeI AGCGCT 1 cut(s) 651
AflII CTTAAG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 2 cut(s) 114, 656
AjiI CACGTC 1 cut(s) 558
AjnI CCWGG 2 cut(s) 59, 582
Alw21I GWGCWC 4 cut(s) 195, 266, 515, 616
Alw26I GTCTC 5 cut(s) 58, 153, 297, 548, 596
Ama87I CYCGRG 2 cut(s) 265, 395
Aor51HI AGCGCT 1 cut(s) 651
AoxI GGCC 1 cut(s) 321
ApeKI GCWGC 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 4 cut(s) 212, 254, 450, 652
AsuC2I CCSGG 3 cut(s) 156, 396, 397
AsuHPI GGTGA 2 cut(s) 17, 379
AsuNHI GCTAGC 1 cut(s) 701
AvaI CYCGRG 2 cut(s) 265, 395
BaeGI GKGCMC 1 cut(s) 530
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 2 cut(s) 234, 515
BauI CACGAG 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 4 cut(s) 195, 266, 515, 616
BbvI GCAGC 1 cut(s) 659
BccI CCATC 3 cut(s) 107, 115, 248
BcgI CGANNNNNNTGC 2 cut(s) 576, 610
BciT130I CCWGG 2 cut(s) 61, 584
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 3 cut(s) 156, 396, 397
BcoDI GTCTC 5 cut(s) 58, 153, 297, 548, 596
BfaI CTAG 2 cut(s) 702, 706
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 1 cut(s) 653
BfrI CTTAAG 1 cut(s) 386
BisI GCNGC 2 cut(s) 455, 648
BlpI GCTNAGC 2 cut(s) 381, 509
BlsI GCNGC 2 cut(s) 456, 649
Bme1390I CCNGG 5 cut(s) 61, 156, 396, 397, 584
BmeT110I CYCGRG 2 cut(s) 265, 395
BmgBI CACGTC 1 cut(s) 558
BmrFI CCNGG 5 cut(s) 61, 156, 396, 397, 584
BmsI GCATC 2 cut(s) 163, 504
BmtI GCTAGC 1 cut(s) 705
BpiI GAAGAC 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 43
Bpu1102I GCTNAGC 2 cut(s) 381, 509
BpuEI CTTGAG 2 cut(s) 283, 335
BpuMI CCSGG 3 cut(s) 156, 396, 397
Bsa29I ATCGAT 1 cut(s) 498
BsaJI CCNNGG 4 cut(s) 154, 279, 395, 475
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 4 cut(s) 41, 57, 71, 87
Bse118I RCCGGY 2 cut(s) 440, 537
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 2 cut(s) 61, 584
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 4 cut(s) 154, 279, 395, 475
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 4 cut(s) 248, 372, 390, 500
BseRI GAGGAG 2 cut(s) 62, 194
BseSI GKGCMC 1 cut(s) 530
BseXI GCAGC 1 cut(s) 659
BsgI GTGCAG 1 cut(s) 644
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 1 cut(s) 323
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 4 cut(s) 195, 266, 515, 616
BsiHKCI CYCGRG 2 cut(s) 265, 395
BsiSI CCGG 5 cut(s) 156, 330, 396, 441, 538
BslFI GGGAC 1 cut(s) 62
BsmAI GTCTC 5 cut(s) 58, 153, 297, 548, 596
BsmBI CGTCTC 1 cut(s) 548
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 1 cut(s) 323
BsoBI CYCGRG 2 cut(s) 265, 395
Bsp1286I GDGCHC 6 cut(s) 195, 234, 266, 515, 530, 616
Bsp143I GATC 4 cut(s) 37, 145, 549, 567
Bsp1720I GCTNAGC 2 cut(s) 381, 509
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 1 cut(s) 454
BspANI GGCC 1 cut(s) 323
BspCNI CTCAG 4 cut(s) 249, 373, 389, 501
BspDI ATCGAT 1 cut(s) 498
BspFNI CGCG 1 cut(s) 450
BspOI GCTAGC 1 cut(s) 705
BspQI GCTCTTC 1 cut(s) 621
BspTI CTTAAG 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 2 cut(s) 440, 537
BssAI RCCGGY 2 cut(s) 440, 537
BssECI CCNNGG 4 cut(s) 154, 279, 395, 475
BssMI GATC 4 cut(s) 37, 145, 549, 567
BssSI CACGAG 1 cut(s) 609
BssT1I CCWWGG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 609
Bst2UI CCWGG 2 cut(s) 61, 584
Bst4CI ACNGT 3 cut(s) 367, 407, 637
Bst6I CTCTTC 2 cut(s) 526, 621
BstAFI CTTAAG 1 cut(s) 386
BstC8I GCNNGC 6 cut(s) 6, 321, 446, 452, 539, 703
BstDEI CTNAG 4 cut(s) 257, 376, 381, 509
BstDSI CCRYGG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 653
BstHHI GCGC 4 cut(s) 212, 254, 450, 652
BstKTI GATC 4 cut(s) 40, 148, 552, 570
BstMAI GTCTC 5 cut(s) 58, 153, 297, 548, 596
BstMBI GATC 4 cut(s) 37, 145, 549, 567
BstMWI GCNNNNNNNGC 5 cut(s) 320, 454, 534, 543, 702
BstNI CCWGG 2 cut(s) 61, 584
BstSCI CCNGG 5 cut(s) 59, 154, 394, 395, 582
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 1 cut(s) 530
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 498
BsuRI GGCC 1 cut(s) 323
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 475
BtrI CACGTC 1 cut(s) 558
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 6 cut(s) 6, 321, 446, 452, 539, 703
CfoI GCGC 4 cut(s) 212, 254, 450, 652
Cfr10I RCCGGY 2 cut(s) 440, 537
Cfr9I CCCGGG 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 498
Csp6I GTAC 1 cut(s) 151
CviAII CATG 3 cut(s) 187, 476, 485
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 4 cut(s) 257, 376, 381, 509
DpnI GATC 4 cut(s) 39, 147, 551, 569
DpnII GATC 4 cut(s) 37, 145, 549, 567
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 2 cut(s) 526, 621
EarI CTCTTC 2 cut(s) 526, 621
Ecl136II GAGCTC 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 475
Eco24I GRGCYC 2 cut(s) 234, 515
Eco47III AGCGCT 1 cut(s) 651
Eco53kI GAGCTC 1 cut(s) 513
Eco57I CTGAAG 2 cut(s) 562, 681
Eco88I CYCGRG 2 cut(s) 265, 395
EcoICRI GAGCTC 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 59, 582
EcoT14I CCWWGG 1 cut(s) 475
EcoT38I GRGCYC 2 cut(s) 234, 515
ErhI CCWWGG 1 cut(s) 475
Esp3I CGTCTC 1 cut(s) 548
FaeI CATG 3 cut(s) 190, 479, 488
FaiI YATR 6 cut(s) 188, 245, 416, 477, 486, 681
FaqI GGGAC 1 cut(s) 62
FatI CATG 3 cut(s) 186, 475, 484
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 455, 648
FriOI GRGCYC 2 cut(s) 234, 515
Fsp4HI GCNGC 2 cut(s) 455, 648
FspBI CTAG 2 cut(s) 702, 706
GlaI GCGC 4 cut(s) 211, 253, 449, 651
GluI GCNGC 2 cut(s) 455, 648
GsuI CTGGAG 1 cut(s) 43
HaeII RGCGCY 1 cut(s) 653
HaeIII GGCC 1 cut(s) 323
HapII CCGG 5 cut(s) 156, 330, 396, 441, 538
HhaI GCGC 4 cut(s) 212, 254, 450, 652
Hin1II CATG 3 cut(s) 190, 479, 488
Hin6I GCGC 4 cut(s) 210, 252, 448, 650
HinP1I GCGC 4 cut(s) 210, 252, 448, 650
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 3 cut(s) 284, 333, 480
HpaII CCGG 5 cut(s) 156, 330, 396, 441, 538
HphI GGTGA 2 cut(s) 17, 379
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 5 cut(s) 258, 283, 430, 564, 601
Hpy188III TCNNGA 6 cut(s) 35, 143, 300, 337, 352, 553
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 2 cut(s) 367, 601
HpyAV CCTTC 1 cut(s) 656
HpyCH4III ACNGT 3 cut(s) 367, 407, 637
HpyCH4IV ACGT 1 cut(s) 557
HpyCH4V TGCA 4 cut(s) 414, 495, 625, 647
HpyF10VI GCNNNNNNNGC 5 cut(s) 320, 454, 534, 543, 702
HpyF3I CTNAG 4 cut(s) 257, 376, 381, 509
HpySE526I ACGT 1 cut(s) 557
Hsp92II CATG 3 cut(s) 190, 479, 488
HspAI GCGC 4 cut(s) 210, 252, 448, 650
KroI GCCGGC 1 cut(s) 537
KroNI GCCGGC 1 cut(s) 539
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 37, 145, 549, 567
LguI GCTCTTC 1 cut(s) 621
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 2 cut(s) 163, 504
MaeI CTAG 2 cut(s) 702, 706
MaeII ACGT 1 cut(s) 557
MaeIII GTNAC 1 cut(s) 470
MalI GATC 4 cut(s) 39, 147, 551, 569
MboI GATC 4 cut(s) 37, 145, 549, 567
MhlI GDGCHC 6 cut(s) 195, 234, 266, 515, 530, 616
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 5 cut(s) 216, 370, 417, 676, 687
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 2 cut(s) 293, 342
MmeI TCCRAC 3 cut(s) 381, 408, 579
Mox20I TGGCCA 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 537
MscI TGGCCA 1 cut(s) 323
MseI TTAA 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 5 cut(s) 156, 330, 396, 441, 538
MspR9I CCNGG 5 cut(s) 61, 156, 396, 397, 584
MvaI CCWGG 2 cut(s) 61, 584
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 5 cut(s) 320, 454, 534, 543, 702
NaeI GCCGGC 1 cut(s) 539
NciI CCSGG 3 cut(s) 156, 396, 397
NcoI CCATGG 1 cut(s) 475
NdeII GATC 4 cut(s) 37, 145, 549, 567
NgoMIV GCCGGC 1 cut(s) 537
NheI GCTAGC 1 cut(s) 701
NlaIII CATG 3 cut(s) 190, 479, 488
NmeAIII GCCGAG 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 621
PdiI GCCGGC 1 cut(s) 539
PfeI GAWTC 1 cut(s) 480
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 2 cut(s) 456, 649
PleI GAGTC 2 cut(s) 292, 341
PpsI GAGTC 2 cut(s) 292, 341
Psp124BI GAGCTC 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 59, 582
PspGI CCWGG 2 cut(s) 59, 582
PspXI VCTCGAGB 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SacI GAGCTC 1 cut(s) 515
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 2 cut(s) 455, 648
Sau3AI GATC 4 cut(s) 37, 145, 549, 567
SchI GAGTC 2 cut(s) 293, 342
ScrFI CCNGG 5 cut(s) 61, 156, 396, 397, 584
SduI GDGCHC 6 cut(s) 195, 234, 266, 515, 530, 616
SfaNI GCATC 2 cut(s) 163, 504
SfcI CTRYAG 1 cut(s) 633
Sfr274I CTCGAG 1 cut(s) 265
SlaI CTCGAG 1 cut(s) 265
SmaI CCCGGG 1 cut(s) 397
SmlI CTYRAG 4 cut(s) 265, 298, 350, 386
SmoI CTYRAG 4 cut(s) 265, 298, 350, 386
Sse9I AATT 5 cut(s) 216, 370, 417, 676, 687
SsiI CCGC 1 cut(s) 454
SspMI CTAG 2 cut(s) 702, 706
SstI GAGCTC 1 cut(s) 515
StyD4I CCNGG 5 cut(s) 59, 154, 394, 395, 582
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 3 cut(s) 367, 407, 637
TaiI ACGT 1 cut(s) 560
TaqI TCGA 4 cut(s) 171, 266, 498, 552
TasI AATT 5 cut(s) 216, 370, 417, 676, 687
TauI GCSGC 1 cut(s) 457
TfiI GAWTC 1 cut(s) 480
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 412
TseI GCWGC 1 cut(s) 647
TspMI CCCGGG 1 cut(s) 395
TspRI CASTG 1 cut(s) 412
Vha464I CTTAAG 1 cut(s) 386
XhoI CTCGAG 1 cut(s) 265
XmaI CCCGGG 1 cut(s) 395
XspI CTAG 2 cut(s) 702, 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.