RchiOBHm_Chr2g0130691

COBRA-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018533
Physical Location & Seq
Forward (+)
46768501 .. 46769153
653 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGACTGCTTTTGCAGAGGCCTATGATCCATTGGATCCCAATGGAAAAATTACTATTCGTTGGGATATAATGAATTGGGCGCCAGATGGATACGAGGCAACCATCACAATGTTTAACTTCCAGAAATATTGGCATATCGAACAGCCAGGTTGGACATTAGGATGGACCTGGGCAAAAAAAGAATTCATATGGTCTATGCTAGGAGGGAAAACAATAGACCGAGGGGACTGCTCAAGTTTCACAGGACCCACTCCACACTGTTGCAAGATGAATCCAAAAGTTGTTGACTTACTTCCTGATGTCGGAAGTTGTTGTAGGGGAGGAGTCATGAGCTCGTTGATTCAGGACACGAGCAAGGCTGTCAGCAGATTCCAGATTACTGTGGGTGGTGCCGGAAGTAACAACGAGCTACTTGTGCTTTGTTACTGCACCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

145

Amino Acids

16.15

Weight (kDa)

5.58

Isoelectric Point (pI)

28.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 31 - 134 4.3e-37 COBRA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018533)

Species Orthologous Gene IDs
pyrus_communis pycom06g18180
rosa_chinensis RchiOBHm_Chr2g0130691
rosa_multiflora Rmu_co8433061.1_g000001 Rmu_sc0002279.1_g000054 Rmu_sc0041315.1_g000001
rosa_roxburghii Rroxscaffold_2G00113710
rosa_samantha Rh2AG344100 Rh2CG331300 Rh2DG370600
rosa_wichuraiana Rw2G027550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 79, 389
AclWI GGATC 3 cut(s) 20, 29, 42
AcsI RAATTY 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 302
AjnI CCWGG 2 cut(s) 145, 167
AluBI AGCT 2 cut(s) 333, 409
AluI AGCT 2 cut(s) 333, 409
Alw21I GWGCWC 1 cut(s) 335
AlwI GGATC 3 cut(s) 20, 29, 42
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 165, 245
AvaII GGWCC 2 cut(s) 165, 245
BamHI GGATCC 1 cut(s) 34
BanI GGYRCC 2 cut(s) 79, 389
BanII GRGCYC 1 cut(s) 335
BauI CACGAG 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 335
BccI CCATC 3 cut(s) 80, 110, 156
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 2 cut(s) 147, 169
BciVI GTATCC 1 cut(s) 83
BfaI CTAG 2 cut(s) 200, 436
BfoI RGCGCY 1 cut(s) 83
BfuI GTATCC 1 cut(s) 83
Bme1390I CCNGG 2 cut(s) 147, 169
Bme18I GGWCC 2 cut(s) 165, 245
BmgT120I GGNCC 2 cut(s) 165, 245
BmiI GGNNCC 4 cut(s) 36, 81, 247, 391
BmrFI CCNGG 2 cut(s) 147, 169
BpuEI CTTGAG 1 cut(s) 217
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 168, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 302
BseBI CCWGG 2 cut(s) 147, 169
BseDI CCNNGG 2 cut(s) 168, 220
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 336
BsgI GTGCAG 1 cut(s) 412
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 2 cut(s) 79, 389
BsiHKAI GWGCWC 1 cut(s) 335
BsiSI CCGG 1 cut(s) 393
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 25, 34
BspANI GGCC 1 cut(s) 20
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 4 cut(s) 36, 81, 247, 391
BspPI GGATC 3 cut(s) 20, 29, 42
BspT107I GGYRCC 2 cut(s) 79, 389
BssECI CCNNGG 2 cut(s) 168, 220
BssMI GATC 2 cut(s) 25, 34
BssNI GRCGYC 1 cut(s) 80
BssSI CACGAG 1 cut(s) 349
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 2 cut(s) 147, 169
Bst4CI ACNGT 2 cut(s) 260, 382
BstACI GRCGYC 1 cut(s) 80
BstF5I GGATG 1 cut(s) 167
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 2 cut(s) 28, 37
BstMBI GATC 2 cut(s) 25, 34
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNI CCWGG 2 cut(s) 147, 169
BstSCI CCNGG 2 cut(s) 145, 167
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 83
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 167
BtsIMutI CAGTG 1 cut(s) 256
CciI TCATGA 1 cut(s) 327
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 165, 245
CviAII CATG 1 cut(s) 328
CviJI RGCY 5 cut(s) 20, 145, 333, 359, 409
CviKI_1 RGCY 5 cut(s) 20, 145, 333, 359, 409
DinI GGCGCC 1 cut(s) 81
DpnI GATC 2 cut(s) 27, 36
DpnII GATC 2 cut(s) 25, 34
Ecl136II GAGCTC 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 2 cut(s) 165, 245
Eco53kI GAGCTC 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 145, 167
EcoT38I GRGCYC 1 cut(s) 335
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
FaeI CATG 1 cut(s) 331
FaiI YATR 7 cut(s) 24, 68, 135, 188, 190, 197, 329
FaqI GGGAC 1 cut(s) 239
FatI CATG 1 cut(s) 327
FauNDI CATATG 1 cut(s) 188
FokI GGATG 1 cut(s) 174
FriOI GRGCYC 1 cut(s) 335
FspBI CTAG 2 cut(s) 200, 436
GlaI GCGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 1 cut(s) 20
HapII CCGG 1 cut(s) 393
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 1 cut(s) 331
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 4 cut(s) 271, 324, 340, 369
HpaII CCGG 1 cut(s) 393
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 5 cut(s) 121, 296, 328, 344, 373
Hpy8I GTNNAC 1 cut(s) 286
HpyCH4III ACNGT 2 cut(s) 260, 382
HpyCH4V TGCA 3 cut(s) 14, 264, 429
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 1 cut(s) 331
HspAI GCGC 1 cut(s) 80
KasI GGCGCC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 25, 34
MaeI CTAG 2 cut(s) 200, 436
MaeIII GTNAC 2 cut(s) 398, 422
MalI GATC 2 cut(s) 27, 36
MboI GATC 2 cut(s) 25, 34
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 3 cut(s) 48, 73, 182
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 2 cut(s) 131, 283
MnlI CCTC 5 cut(s) 10, 88, 197, 215, 314
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 2 cut(s) 107, 160
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 2 cut(s) 147, 169
MvaI CCWGG 2 cut(s) 147, 169
MwoI GCNNNNNNNGC 1 cut(s) 415
NarI GGCGCC 1 cut(s) 80
NdeI CATATG 1 cut(s) 188
NdeII GATC 2 cut(s) 25, 34
NlaIII CATG 1 cut(s) 331
NlaIV GGNNCC 4 cut(s) 36, 81, 247, 391
PagI TCATGA 1 cut(s) 327
PceI AGGCCT 1 cut(s) 20
PfeI GAWTC 3 cut(s) 271, 340, 369
PleI GAGTC 1 cut(s) 332
PluTI GGCGCC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 332
PpuMI RGGWCCY 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 145, 167
PspGI CCWGG 2 cut(s) 145, 167
PspN4I GGNNCC 4 cut(s) 36, 81, 247, 391
PspPI GGNCC 2 cut(s) 165, 245
PspPPI RGGWCCY 1 cut(s) 245
PsuI RGATCY 1 cut(s) 34
RseI CAYNNNNRTG 2 cut(s) 107, 160
SacI GAGCTC 1 cut(s) 335
SaqAI TTAA 1 cut(s) 114
Sau3AI GATC 2 cut(s) 25, 34
Sau96I GGNCC 2 cut(s) 165, 245
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 2 cut(s) 147, 169
SduI GDGCHC 1 cut(s) 335
SetI ASST 4 cut(s) 151, 170, 335, 411
SfoI GGCGCC 1 cut(s) 81
SinI GGWCC 2 cut(s) 165, 245
SmiMI CAYNNNNRTG 2 cut(s) 107, 160
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 3 cut(s) 48, 73, 182
SseBI AGGCCT 1 cut(s) 20
SspDI GGCGCC 1 cut(s) 79
SspI AATATT 1 cut(s) 128
SspMI CTAG 2 cut(s) 200, 436
SstI GAGCTC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 2 cut(s) 145, 167
TaaI ACNGT 2 cut(s) 260, 382
TaqI TCGA 1 cut(s) 138
TaqII GACCGA 1 cut(s) 234
TasI AATT 3 cut(s) 48, 73, 182
TfiI GAWTC 3 cut(s) 271, 340, 369
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 86, 175, 284
TspRI CASTG 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 165, 245
XapI RAATTY 1 cut(s) 182
XspI CTAG 2 cut(s) 200, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.