RchiOBHm_Chr2g0153481

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009811
Physical Location & Seq
Reverse (-)
70783238 .. 70785447
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
ATGAACAAGTTTCAGAAGAATGGCCTCCTCTCGTTTTTCTGCCTCCTCCTTTTACTCCCAATCTTCGCTTCCGCTGAGTGTACGTGCGACGAGGCCGAAGATGAAGGTGACAGCAACAAAAGCAAAGCTCTCAAATACAAACTCGGCGCATTCGCTTCCATACTCGTCGCCAGTACAGTCGGTGTTTGTATCCCAATTCTAGGGAAGACAATTCCGGCTCTGCAACCGGACAAGAATATCTTCTTCATCGTCAAGGCTTTCGCCGCCGGCGTCATATTGGCCACCGGATTCATCCACGTGCTTCCGGACGCTTTCGACAACTTGACGTCGCCGTGCCTGAAAGAAAACCCGTGGGGGAAGTTTCCCTTCACCGGATTTGTTGCCATGATGGCGGCCATCGGAACTTTGATGGTGGATTCCATTGCTACTTCTTATTTCAGCGGGTCTCACTTCAATACGGCTCAGAATCAGATTAATGGGGATGAGGAGAAAGAGGGACAGCACGAGGGGCACGTACATGTTCATACACATGGCACTCATGGTCATTCTCATGGTTCGCTTGATACGAATTCGGCTGAATCACAACTTCTTCGGCACAGAGTGATTTCAAAGGTGTTGGAGTTGGGAATTGTGGTGCACTCGGTAATTATTGGAATTTCATTGGGTGTTTCTGAAAGTCCCGATACAATTCGACCTCTTGTAGCTGCTTTGACTTTCCATCAATTTTTTGAAGGCATGGGACTTGGTGGGTGCATAGCTCAGGCGAGAGAATCACGAATAACAACTATAATCATGGTGCTGTTCTTCTCCCTTACAACCCCTGTTGGAATCGCGATTGGAATTGGAATATCAAGCCATTACAAGGAAAACAGTCCAACTGCTCTCATACTTGAAGGAACTTTTAATGCGGCATCAGCTGGGATCTTAATCTACATGTCTCTGGTCGATCTGCTAGCGGCTGATTTTATGAACCCGAAAATGCAGAAGAATGTGAAGCTTCAAATTGGGGCTAATGCTTCACTTCTTTTTGGAGCTGGTTGCATGTCTCTTATGGCCAAATGGGCTTAA

Protein Analysis

355

Amino Acids

37.94

Weight (kDa)

6.26

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 45 - 352 3.5e-82 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 329
AccII CGCG 1 cut(s) 833
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 6 cut(s) 72, 264, 392, 441, 908, 956
AclWI GGATC 1 cut(s) 929
AcoI YGGCCR 3 cut(s) 279, 393, 1053
AcsI RAATTY 2 cut(s) 568, 654
AcvI CACGTG 1 cut(s) 298
AcyI GRCGYC 2 cut(s) 270, 326
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 3 cut(s) 82, 175, 516
AfiI CCNNNNNNNGG 3 cut(s) 200, 371, 862
AflIII ACRYGT 2 cut(s) 517, 933
AgsI TTSAA 5 cut(s) 454, 609, 731, 893, 1001
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 6 cut(s) 128, 704, 758, 917, 997, 1034
AluI AGCT 6 cut(s) 128, 704, 758, 917, 997, 1034
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 3 cut(s) 450, 942, 1050
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 1 cut(s) 929
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 5 cut(s) 22, 93, 279, 393, 1053
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 1 cut(s) 704
ApoI RAATTY 2 cut(s) 568, 654
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 119, 361
AsuNHI GCTAGC 1 cut(s) 952
BaeGI GKGCMC 2 cut(s) 513, 639
BalI TGGCCA 2 cut(s) 281, 1055
BauI CACGAG 1 cut(s) 503
BbrPI CACGTG 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 1 cut(s) 691
BccI CCATC 4 cut(s) 382, 403, 404, 726
BceAI ACGGC 2 cut(s) 316, 474
BciVI GTATCC 1 cut(s) 200
BcoDI GTCTC 3 cut(s) 450, 942, 1050
BfaI CTAG 2 cut(s) 200, 953
BfuI GTATCC 1 cut(s) 200
BglI GCCNNNNNGGC 2 cut(s) 389, 1061
BisI GCNGC 5 cut(s) 264, 393, 705, 909, 957
BlsI GCNGC 5 cut(s) 265, 394, 706, 910, 958
BmsI GCATC 1 cut(s) 920
BmtI GCTAGC 1 cut(s) 956
BpiI GAAGAC 1 cut(s) 212
Bpu10I CCTNAGC 1 cut(s) 759
BsaAI YACGTR 3 cut(s) 84, 298, 514
BsaBI GATNNNNATC 1 cut(s) 926
BsaHI GRCGYC 2 cut(s) 270, 326
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 350
BsaWI WCCGGW 4 cut(s) 226, 284, 304, 371
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 371, 862
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 926
BseAI TCCGGA 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 350
BseGI GGATG 2 cut(s) 291, 487
BseJI GATNNNNATC 1 cut(s) 926
BseLI CCNNNNNNNGG 3 cut(s) 200, 371, 862
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 3 cut(s) 66, 476, 773
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 3 cut(s) 17, 35, 500
BseSI GKGCMC 2 cut(s) 513, 639
BseXI GCAGC 1 cut(s) 691
BseYI CCCAGC 1 cut(s) 917
Bsh1236I CGCG 1 cut(s) 833
BshFI GGCC 5 cut(s) 24, 95, 281, 395, 1055
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 6 cut(s) 215, 227, 267, 285, 305, 372
BslFI GGGAC 3 cut(s) 510, 663, 753
BslI CCNNNNNNNGG 3 cut(s) 200, 371, 862
BsmAI GTCTC 3 cut(s) 450, 942, 1050
BsmFI GGGAC 3 cut(s) 510, 663, 753
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 5 cut(s) 24, 95, 281, 395, 1055
Bso31I GGTCTC 1 cut(s) 450
Bsp1286I GDGCHC 2 cut(s) 513, 639
Bsp13I TCCGGA 1 cut(s) 304
Bsp143I GATC 2 cut(s) 921, 946
Bsp68I TCGCGA 1 cut(s) 833
BspACI CCGC 6 cut(s) 72, 264, 392, 441, 908, 956
BspANI GGCC 5 cut(s) 24, 95, 281, 395, 1055
BspCNI CTCAG 3 cut(s) 67, 475, 772
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 1 cut(s) 833
BspOI GCTAGC 1 cut(s) 956
BspPI GGATC 1 cut(s) 929
BspTNI GGTCTC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 171
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 350
BssMI GATC 2 cut(s) 921, 946
BssNI GRCGYC 2 cut(s) 270, 326
BssSI CACGAG 1 cut(s) 503
Bst2BI CACGAG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 178, 872
BstACI GRCGYC 2 cut(s) 270, 326
BstBAI YACGTR 3 cut(s) 84, 298, 514
BstC8I GCNNGC 2 cut(s) 268, 954
BstDEI CTNAG 3 cut(s) 75, 462, 759
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 291, 487
BstFNI CGCG 1 cut(s) 833
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 924, 949
BstMAI GTCTC 3 cut(s) 450, 942, 1050
BstMBI GATC 2 cut(s) 921, 946
BstMWI GCNNNNNNNGC 6 cut(s) 120, 263, 389, 508, 914, 1061
BstNSI RCATGY 3 cut(s) 521, 937, 1045
BstSLI GKGCMC 2 cut(s) 513, 639
BstUI CGCG 1 cut(s) 833
BstV1I GCAGC 1 cut(s) 691
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 921
BstYI RGATCY 1 cut(s) 921
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 5 cut(s) 24, 95, 281, 395, 1055
BtgI CCRYGG 1 cut(s) 350
BtsCI GGATG 2 cut(s) 291, 487
BtuMI TCGCGA 1 cut(s) 833
Cac8I GCNNGC 2 cut(s) 268, 954
CfoI GCGC 1 cut(s) 149
Cfr10I RCCGGY 1 cut(s) 266
CseI GACGC 2 cut(s) 259, 317
Csp6I GTAC 3 cut(s) 81, 174, 515
CviAII CATG 9 cut(s) 385, 518, 530, 539, 551, 736, 793, 934, 1042
CviQI GTAC 3 cut(s) 81, 174, 515
DdeI CTNAG 3 cut(s) 75, 462, 759
DpnI GATC 2 cut(s) 923, 948
DpnII GATC 2 cut(s) 921, 946
DraIII CACNNNGTG 1 cut(s) 601
EaeI YGGCCR 3 cut(s) 279, 393, 1053
Eco31I GGTCTC 1 cut(s) 450
Eco72I CACGTG 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 568
FaeI CATG 9 cut(s) 388, 521, 533, 542, 554, 739, 796, 937, 1045
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 350, 382
FaqI GGGAC 3 cut(s) 510, 663, 753
FatI CATG 9 cut(s) 384, 517, 529, 538, 550, 735, 792, 933, 1041
FauI CCCGC 1 cut(s) 434
Fnu4HI GCNGC 5 cut(s) 264, 393, 705, 909, 957
FokI GGATG 2 cut(s) 278, 494
Fsp4HI GCNGC 5 cut(s) 264, 393, 705, 909, 957
FspBI CTAG 2 cut(s) 200, 953
GlaI GCGC 1 cut(s) 148
GluI GCNGC 5 cut(s) 264, 393, 705, 909, 957
GsaI CCCAGC 1 cut(s) 921
HaeIII GGCC 5 cut(s) 24, 95, 281, 395, 1055
HapII CCGG 6 cut(s) 215, 227, 267, 285, 305, 372
HgaI GACGC 2 cut(s) 259, 317
HhaI GCGC 1 cut(s) 149
Hin1I GRCGYC 2 cut(s) 270, 326
Hin1II CATG 9 cut(s) 388, 521, 533, 542, 554, 739, 796, 937, 1045
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 6 cut(s) 288, 416, 466, 578, 770, 828
HpaII CCGG 6 cut(s) 215, 227, 267, 285, 305, 372
HphI GGTGA 2 cut(s) 119, 361
Hpy166II GTNNAC 2 cut(s) 81, 637
Hpy188I TCNGA 5 cut(s) 15, 401, 465, 471, 673
Hpy188III TCNNGA 4 cut(s) 305, 680, 774, 832
Hpy8I GTNNAC 2 cut(s) 81, 637
Hpy99I CGWCG 3 cut(s) 92, 170, 331
HpyAV CCTTC 4 cut(s) 98, 376, 725, 887
HpyCH4III ACNGT 2 cut(s) 178, 872
HpyCH4IV ACGT 4 cut(s) 83, 297, 326, 513
HpyCH4V TGCA 5 cut(s) 223, 637, 753, 982, 1041
HpyF10VI GCNNNNNNNGC 6 cut(s) 120, 263, 389, 508, 914, 1061
HpyF3I CTNAG 3 cut(s) 75, 462, 759
HpySE526I ACGT 4 cut(s) 83, 297, 326, 513
Hsp92I GRCGYC 2 cut(s) 270, 326
Hsp92II CATG 9 cut(s) 388, 521, 533, 542, 554, 739, 796, 937, 1045
HspAI GCGC 1 cut(s) 147
Kpn2I TCCGGA 1 cut(s) 304
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Kzo9I GATC 2 cut(s) 921, 946
LmnI GCTCC 1 cut(s) 1031
Lsp1109I GCAGC 1 cut(s) 691
LweI GCATC 1 cut(s) 920
MaeI CTAG 2 cut(s) 200, 953
MaeII ACGT 4 cut(s) 83, 297, 326, 513
MaeIII GTNAC 1 cut(s) 107
MalI GATC 2 cut(s) 923, 948
MboI GATC 2 cut(s) 921, 946
MboII GAAGA 9 cut(s) 28, 55, 110, 217, 232, 235, 581, 796, 997
MflI RGATCY 1 cut(s) 921
MhlI GDGCHC 2 cut(s) 513, 639
MlsI TGGCCA 2 cut(s) 281, 1055
MluNI TGGCCA 2 cut(s) 281, 1055
MmeI TCCRAC 3 cut(s) 597, 805, 899
MnlI CCTC 9 cut(s) 35, 38, 53, 56, 85, 478, 487, 499, 705
Mox20I TGGCCA 2 cut(s) 281, 1055
MreI CGCCGGCG 1 cut(s) 266
MroI TCCGGA 1 cut(s) 304
MroNI GCCGGC 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 239
MscI TGGCCA 2 cut(s) 281, 1055
MseI TTAA 4 cut(s) 474, 903, 926, 1066
MslI CAYNNNNRTG 4 cut(s) 296, 516, 528, 549
Msp20I TGGCCA 2 cut(s) 281, 1055
MspA1I CMGCKG 3 cut(s) 74, 441, 917
MspI CCGG 6 cut(s) 215, 227, 267, 285, 305, 372
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 1 cut(s) 833
MwoI GCNNNNNNNGC 6 cut(s) 120, 263, 389, 508, 914, 1061
NaeI GCCGGC 1 cut(s) 268
NdeII GATC 2 cut(s) 921, 946
NgoMIV GCCGGC 1 cut(s) 266
NheI GCTAGC 1 cut(s) 952
NlaIII CATG 9 cut(s) 388, 521, 533, 542, 554, 739, 796, 937, 1045
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 107
NruI TCGCGA 1 cut(s) 833
NspI RCATGY 3 cut(s) 521, 937, 1045
PciI ACATGT 2 cut(s) 517, 933
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 510, 563
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 6 cut(s) 288, 416, 466, 578, 770, 828
PkrI GCNGC 5 cut(s) 265, 394, 706, 910, 958
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
Ppu21I YACGTR 3 cut(s) 84, 298, 514
PscI ACATGT 2 cut(s) 517, 933
PshBI ATTAAT 1 cut(s) 474
PspCI CACGTG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 917
PsuI RGATCY 1 cut(s) 921
PvuII CAGCTG 1 cut(s) 917
RruI TCGCGA 1 cut(s) 833
RsaI GTAC 3 cut(s) 82, 175, 516
RsaNI GTAC 3 cut(s) 81, 174, 515
RseI CAYNNNNRTG 4 cut(s) 296, 516, 528, 549
SaqAI TTAA 4 cut(s) 474, 903, 926, 1066
SatI GCNGC 5 cut(s) 264, 393, 705, 909, 957
Sau3AI GATC 2 cut(s) 921, 946
SduI GDGCHC 2 cut(s) 513, 639
SfaNI GCATC 1 cut(s) 920
SgrAI CRCCGGYG 1 cut(s) 266
SmiMI CAYNNNNRTG 4 cut(s) 296, 516, 528, 549
SsiI CCGC 6 cut(s) 72, 264, 392, 441, 908, 956
SspMI CTAG 2 cut(s) 200, 953
TaaI ACNGT 2 cut(s) 178, 872
TaiI ACGT 4 cut(s) 86, 300, 329, 516
TaqI TCGA 3 cut(s) 315, 691, 945
TatI WGTACW 1 cut(s) 173
TauI GCSGC 4 cut(s) 266, 395, 911, 959
TfiI GAWTC 6 cut(s) 288, 416, 466, 578, 770, 828
Tru1I TTAA 4 cut(s) 474, 903, 926, 1066
Tru9I TTAA 4 cut(s) 474, 903, 926, 1066
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 1 cut(s) 704
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 7 cut(s) 17, 117, 235, 280, 512, 648, 983
VneI GTGCAC 1 cut(s) 635
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 2 cut(s) 568, 654
XceI RCATGY 3 cut(s) 521, 937, 1045
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 2 cut(s) 200, 953
ZraI GACGTC 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.