Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_4g20951 |
| rosa_chinensis | RchiOBHm_Chr2g0161521 RchiOBHm_Chr3g0478151 |
| rosa_multiflora | Rmu_sc0007744.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00053410 |
| rosa_samantha | Rh3AG215300 Rh3BG248900 Rh5AG180600 Rh5BG179200 |
| rosa_wichuraiana | Rw2G046050 Rw3G019570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 175 |
| AciI | CCGC | 1 cut(s) | 150 |
| AclWI | GGATC | 2 cut(s) | 148, 161 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 149, 163 |
| AgsI | TTSAA | 1 cut(s) | 92 |
| AluBI | AGCT | 1 cut(s) | 57 |
| AluI | AGCT | 1 cut(s) | 57 |
| Alw21I | GWGCWC | 2 cut(s) | 59, 171 |
| Alw44I | GTGCAC | 1 cut(s) | 167 |
| AlwI | GGATC | 2 cut(s) | 148, 161 |
| ApaLI | GTGCAC | 1 cut(s) | 167 |
| AspS9I | GGNCC | 2 cut(s) | 115, 140 |
| AvaII | GGWCC | 2 cut(s) | 115, 140 |
| BaeGI | GKGCMC | 1 cut(s) | 171 |
| BamHI | GGATCC | 1 cut(s) | 153 |
| BanII | GRGCYC | 1 cut(s) | 59 |
| BbsI | GAAGAC | 1 cut(s) | 23 |
| Bbv12I | GWGCWC | 2 cut(s) | 59, 171 |
| Bme18I | GGWCC | 2 cut(s) | 115, 140 |
| BmgT120I | GGNCC | 2 cut(s) | 115, 140 |
| BmiI | GGNNCC | 1 cut(s) | 155 |
| BpiI | GAAGAC | 1 cut(s) | 23 |
| BpmI | CTGGAG | 1 cut(s) | 65 |
| BsaWI | WCCGGW | 2 cut(s) | 112, 142 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 149, 163 |
| Bse1I | ACTGG | 1 cut(s) | 168 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 149, 163 |
| BseNI | ACTGG | 1 cut(s) | 168 |
| BseSI | GKGCMC | 1 cut(s) | 171 |
| Bsh1285I | CGRYCG | 1 cut(s) | 179 |
| BsiEI | CGRYCG | 1 cut(s) | 179 |
| BsiHKAI | GWGCWC | 2 cut(s) | 59, 171 |
| BsiSI | CCGG | 2 cut(s) | 113, 143 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 149, 163 |
| Bsp1286I | GDGCHC | 2 cut(s) | 59, 171 |
| Bsp143I | GATC | 3 cut(s) | 129, 153, 186 |
| BspACI | CCGC | 1 cut(s) | 150 |
| BspLI | GGNNCC | 1 cut(s) | 155 |
| BspPI | GGATC | 2 cut(s) | 148, 161 |
| BsrI | ACTGG | 1 cut(s) | 168 |
| BssMI | GATC | 3 cut(s) | 129, 153, 186 |
| Bst4CI | ACNGT | 1 cut(s) | 173 |
| BstKTI | GATC | 3 cut(s) | 132, 156, 189 |
| BstMBI | GATC | 3 cut(s) | 129, 153, 186 |
| BstMCI | CGRYCG | 1 cut(s) | 179 |
| BstSLI | GKGCMC | 1 cut(s) | 171 |
| BstV2I | GAAGAC | 1 cut(s) | 23 |
| BstX2I | RGATCY | 1 cut(s) | 153 |
| BstYI | RGATCY | 1 cut(s) | 153 |
| Cfr13I | GGNCC | 2 cut(s) | 115, 140 |
| CviJI | RGCY | 1 cut(s) | 57 |
| CviKI_1 | RGCY | 1 cut(s) | 57 |
| DpnI | GATC | 3 cut(s) | 131, 155, 188 |
| DpnII | GATC | 3 cut(s) | 129, 153, 186 |
| Ecl136II | GAGCTC | 1 cut(s) | 57 |
| Eco24I | GRGCYC | 1 cut(s) | 59 |
| Eco47I | GGWCC | 2 cut(s) | 115, 140 |
| Eco53kI | GAGCTC | 1 cut(s) | 57 |
| EcoICRI | GAGCTC | 1 cut(s) | 57 |
| EcoT38I | GRGCYC | 1 cut(s) | 59 |
| FauI | CCCGC | 1 cut(s) | 143 |
| FblI | GTMKAC | 1 cut(s) | 175 |
| FriOI | GRGCYC | 1 cut(s) | 59 |
| GsuI | CTGGAG | 1 cut(s) | 65 |
| HapII | CCGG | 2 cut(s) | 113, 143 |
| HincII | GTYRAC | 1 cut(s) | 176 |
| HindII | GTYRAC | 1 cut(s) | 176 |
| HinfI | GANTC | 2 cut(s) | 88, 105 |
| HpaII | CCGG | 2 cut(s) | 113, 143 |
| Hpy166II | GTNNAC | 2 cut(s) | 169, 176 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 194 |
| Hpy188III | TCNNGA | 2 cut(s) | 44, 92 |
| Hpy8I | GTNNAC | 2 cut(s) | 169, 176 |
| Hpy99I | CGWCG | 1 cut(s) | 177 |
| HpyCH4III | ACNGT | 1 cut(s) | 173 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 169 |
| Kzo9I | GATC | 3 cut(s) | 129, 153, 186 |
| LmnI | GCTCC | 1 cut(s) | 62 |
| LpnPI | CCDG | 7 cut(s) | 12, 29, 123, 126, 131, 149, 156 |
| MalI | GATC | 3 cut(s) | 131, 155, 188 |
| MboI | GATC | 3 cut(s) | 129, 153, 186 |
| MboII | GAAGA | 2 cut(s) | 28, 181 |
| MflI | RGATCY | 1 cut(s) | 153 |
| MhlI | GDGCHC | 2 cut(s) | 59, 171 |
| MnlI | CCTC | 2 cut(s) | 40, 117 |
| MspI | CCGG | 2 cut(s) | 113, 143 |
| NdeII | GATC | 3 cut(s) | 129, 153, 186 |
| NlaIV | GGNNCC | 1 cut(s) | 155 |
| PfeI | GAWTC | 2 cut(s) | 88, 105 |
| PflFI | GACNNNGTC | 1 cut(s) | 74 |
| Psp124BI | GAGCTC | 1 cut(s) | 59 |
| PspN4I | GGNNCC | 1 cut(s) | 155 |
| PspPI | GGNCC | 2 cut(s) | 115, 140 |
| PsuI | RGATCY | 1 cut(s) | 153 |
| PsyI | GACNNNGTC | 1 cut(s) | 74 |
| SacI | GAGCTC | 1 cut(s) | 59 |
| SalI | GTCGAC | 1 cut(s) | 174 |
| Sau3AI | GATC | 3 cut(s) | 129, 153, 186 |
| Sau96I | GGNCC | 2 cut(s) | 115, 140 |
| SduI | GDGCHC | 2 cut(s) | 59, 171 |
| SetI | ASST | 5 cut(s) | 9, 51, 59, 128, 185 |
| SinI | GGWCC | 2 cut(s) | 115, 140 |
| SsiI | CCGC | 1 cut(s) | 150 |
| SstI | GAGCTC | 1 cut(s) | 59 |
| TaaI | ACNGT | 1 cut(s) | 173 |
| TaqI | TCGA | 1 cut(s) | 175 |
| TaqII | GACCGA | 1 cut(s) | 193 |
| TfiI | GAWTC | 2 cut(s) | 88, 105 |
| Tth111I | GACNNNGTC | 1 cut(s) | 74 |
| VneI | GTGCAC | 1 cut(s) | 167 |
| VpaK11BI | GGWCC | 2 cut(s) | 115, 140 |
| XmiI | GTMKAC | 1 cut(s) | 175 |