Rh3AG215300

Ubiquitin-2 like Rad60 SUMO-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0017872
Physical Location & Seq
Forward (+)
21687111 .. 21697503
10393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG215300.1

Sequence Viewer

Length: 552 bp
ATGCAGATTTTCGTGAAAACTCTAACTGGGAAGACCATCACTCTCGAGGTTGAGAGCAGCGACACCATCGATAATGTCAAAGCCAAGATCCAGGATAAGGAAGGCATCCCTCCTGATCAACAGAGATTGATTTTTGCCGGTAAGCAATTGGAAGATGGTAGGACTCTTGCAGATTATAACATCCAAAAGGAGTCAACTCTTCACCTGGTTTTGAGGCTGAGGGGAGGAACTATGATTAAGGTGAAGACTCTTACCGGGAAAGAAATTGAGATTGATATTGAACCGACTGACACCATTGACCGAATTAAGGAAAGGGTTGAGGAGAAAGAGGGAATTCCTCCGGTGCAACAAAGGTTTTTTCCTGTGACACAAGTTCTACTTTCTAATCCTGGAAACTCAAGGAATCAGCTTGCAAGAATGCTAGCCATTGATTCAAGTGGCTGCTTCATTGCTGCTAGTGCATATGTAGACCGGTTGGCTATGTTCTCTGTTTCAATGCCTGGAGGAAGGTTAAGCTGCTACTTTATCCCTCTTCTTATGTGCCGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

183

Amino Acids

20.49

Weight (kDa)

7.67

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 2.1e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 2.7e-32 Ubiquitin family
ubiquitin PF00240 79 - 120 2e-11 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017872)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20951
rosa_chinensis RchiOBHm_Chr2g0161521 RchiOBHm_Chr3g0478151
rosa_multiflora Rmu_sc0007744.1_g000001
rosa_roxburghii Rroxscaffold_1G00053410
rosa_samantha Rh3AG215300 Rh3BG248900 Rh5AG180600 Rh5BG179200
rosa_wichuraiana Rw2G046050 Rw3G019570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccI GTMKAC 1 cut(s) 468
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 333
AfiI CCNNNNNNNGG 2 cut(s) 97, 307
AgeI ACCGGT 1 cut(s) 471
AgsI TTSAA 3 cut(s) 281, 435, 495
AjnI CCWGG 4 cut(s) 90, 204, 388, 499
AluBI AGCT 2 cut(s) 409, 516
AluI AGCT 2 cut(s) 409, 516
AlwI GGATC 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 44
ApeKI GCWGC 4 cut(s) 57, 441, 452, 516
ApoI RAATTY 1 cut(s) 333
AsiGI ACCGGT 1 cut(s) 471
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 2 cut(s) 194, 253
AsuNHI GCTAGC 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 44
BbsI GAAGAC 2 cut(s) 38, 251
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 4 cut(s) 69, 428, 439, 503
BccI CCATC 3 cut(s) 44, 74, 149
BciT130I CCWGG 4 cut(s) 92, 206, 390, 501
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 1 cut(s) 256
BfaI CTAG 3 cut(s) 422, 456, 550
BisI GCNGC 4 cut(s) 58, 442, 453, 517
BlsI GCNGC 4 cut(s) 59, 443, 454, 518
Bme1390I CCNGG 5 cut(s) 92, 206, 256, 390, 501
BmeT110I CYCGRG 1 cut(s) 44
BmrFI CCNGG 5 cut(s) 92, 206, 256, 390, 501
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 114
BmtI GCTAGC 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 38, 251
BpmI CTGGAG 1 cut(s) 522
Bpu10I CCTNAGC 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 256
Bsa29I ATCGAT 1 cut(s) 69
BsaWI WCCGGW 2 cut(s) 340, 471
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 307
Bse118I RCCGGY 2 cut(s) 137, 471
Bse1I ACTGG 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 4 cut(s) 92, 206, 390, 501
BseCI ATCGAT 1 cut(s) 69
BseGI GGATG 2 cut(s) 105, 180
BseLI CCNNNNNNNGG 2 cut(s) 97, 307
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 31
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 4 cut(s) 69, 428, 439, 503
BshTI ACCGGT 1 cut(s) 471
BshVI ATCGAT 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 4 cut(s) 138, 255, 341, 472
BslI CCNNNNNNNGG 2 cut(s) 97, 307
BsmI GAATGC 1 cut(s) 423
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 2 cut(s) 87, 115
BspCNI CTCAG 1 cut(s) 210
BspDI ATCGAT 1 cut(s) 69
BspOI GCTAGC 1 cut(s) 425
BspPI GGATC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 447
BsrFI RCCGGY 2 cut(s) 137, 471
BsrI ACTGG 1 cut(s) 31
BssAI RCCGGY 2 cut(s) 137, 471
BssMI GATC 2 cut(s) 87, 115
Bst2UI CCWGG 4 cut(s) 92, 206, 390, 501
Bst6I CTCTTC 2 cut(s) 204, 537
BstC8I GCNNGC 2 cut(s) 411, 423
BstDEI CTNAG 1 cut(s) 218
BstF5I GGATG 2 cut(s) 105, 180
BstKTI GATC 2 cut(s) 90, 118
BstMBI GATC 2 cut(s) 87, 115
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 4 cut(s) 92, 206, 390, 501
BstSCI CCNGG 5 cut(s) 90, 204, 254, 388, 499
BstV1I GCAGC 4 cut(s) 69, 428, 439, 503
BstV2I GAAGAC 2 cut(s) 38, 251
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 69
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 2 cut(s) 105, 180
Cac8I GCNNGC 2 cut(s) 411, 423
Cfr10I RCCGGY 2 cut(s) 137, 471
ClaI ATCGAT 1 cut(s) 69
CsiI ACCWGGT 1 cut(s) 204
CspAI ACCGGT 1 cut(s) 471
CviJI RGCY 7 cut(s) 83, 217, 409, 425, 441, 479, 516
CviKI_1 RGCY 7 cut(s) 83, 217, 409, 425, 441, 479, 516
DdeI CTNAG 1 cut(s) 218
DpnI GATC 2 cut(s) 89, 117
DpnII GATC 2 cut(s) 87, 115
Eam1104I CTCTTC 2 cut(s) 204, 537
EarI CTCTTC 2 cut(s) 204, 537
Eco88I CYCGRG 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 4 cut(s) 90, 204, 388, 499
FaiI YATR 6 cut(s) 177, 233, 463, 465, 482, 539
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 4 cut(s) 58, 442, 453, 517
FokI GGATG 2 cut(s) 92, 167
Fsp4HI GCNGC 4 cut(s) 58, 442, 453, 517
FspBI CTAG 3 cut(s) 422, 456, 550
GluI GCNGC 4 cut(s) 58, 442, 453, 517
GsuI CTGGAG 1 cut(s) 522
HapII CCGG 4 cut(s) 138, 255, 341, 472
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 5 cut(s) 163, 191, 247, 403, 431
HpaII CCGG 4 cut(s) 138, 255, 341, 472
HphI GGTGA 2 cut(s) 194, 253
Hpy166II GTNNAC 2 cut(s) 195, 469
Hpy188III TCNNGA 3 cut(s) 13, 44, 113
Hpy8I GTNNAC 2 cut(s) 195, 469
HpyAV CCTTC 2 cut(s) 95, 501
HpyCH4V TGCA 5 cut(s) 4, 170, 346, 413, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 1 cut(s) 218
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 87, 115
Lsp1109I GCAGC 4 cut(s) 69, 428, 439, 503
LweI GCATC 1 cut(s) 114
MabI ACCWGGT 1 cut(s) 204
MaeI CTAG 3 cut(s) 422, 456, 550
MaeIII GTNAC 1 cut(s) 364
MalI GATC 2 cut(s) 89, 117
MboI GATC 2 cut(s) 87, 115
MboII GAAGA 5 cut(s) 43, 164, 191, 256, 524
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 1 cut(s) 87
MluCI AATT 4 cut(s) 146, 264, 303, 333
MlyI GAGTC 3 cut(s) 157, 200, 241
MseI TTAA 3 cut(s) 237, 306, 512
MspI CCGG 4 cut(s) 138, 255, 341, 472
MspR9I CCNGG 5 cut(s) 92, 206, 256, 390, 501
MunI CAATTG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 423
MvaI CCWGG 4 cut(s) 92, 206, 390, 501
MwoI GCNNNNNNNGC 1 cut(s) 458
NciI CCSGG 1 cut(s) 256
NdeI CATATG 1 cut(s) 463
NdeII GATC 2 cut(s) 87, 115
NheI GCTAGC 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 364
PaeR7I CTCGAG 1 cut(s) 44
PctI GAATGC 1 cut(s) 423
PfeI GAWTC 2 cut(s) 403, 431
PfoI TCCNGGA 2 cut(s) 90, 388
PinAI ACCGGT 1 cut(s) 471
PkrI GCNGC 4 cut(s) 59, 443, 454, 518
PleI GAGTC 3 cut(s) 157, 199, 241
PpsI GAGTC 3 cut(s) 157, 199, 241
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 4 cut(s) 90, 204, 388, 499
PspGI CCWGG 4 cut(s) 90, 204, 388, 499
PsuI RGATCY 1 cut(s) 87
SaqAI TTAA 3 cut(s) 237, 306, 512
SatI GCNGC 4 cut(s) 58, 442, 453, 517
Sau3AI GATC 2 cut(s) 87, 115
SchI GAGTC 3 cut(s) 157, 200, 241
ScrFI CCNGG 5 cut(s) 92, 206, 256, 390, 501
SetI ASST 7 cut(s) 51, 207, 243, 356, 411, 512, 518
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 1 cut(s) 114
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 44, 397
SmoI CTYRAG 2 cut(s) 44, 397
Sse9I AATT 4 cut(s) 146, 264, 303, 333
SspMI CTAG 3 cut(s) 422, 456, 550
StyD4I CCNGG 5 cut(s) 90, 204, 254, 388, 499
TaqI TCGA 2 cut(s) 45, 69
TaqII GACCGA 1 cut(s) 315
TasI AATT 4 cut(s) 146, 264, 303, 333
TfiI GAWTC 2 cut(s) 403, 431
Tru1I TTAA 3 cut(s) 237, 306, 512
Tru9I TTAA 3 cut(s) 237, 306, 512
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 4 cut(s) 57, 441, 452, 516
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 436
XapI RAATTY 1 cut(s) 333
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 3 cut(s) 422, 456, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.