RchiOBHm_Chr2g0163471

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0016903
Physical Location & Seq
Reverse (-)
78956496 .. 78958943
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGGCAATGGAAGAAGTGGTGCTGCCAAAGCCATTGATGATCTCTTTAGAGGAAGAACACAAAAACTGCGTAAACTACGCATTCAGGTGCAGTTGGGATATTTCAAGAGAGAAGAATCTGGTTTGTGTGACCAGTGGGAATTCTTACCTGGGTTCTCACATAGTTAGGGCTCTTTTGGCTCGCGGATATCGGATTCGAGTCACCATTCAAAACCAAGAGGATTATGAAGACATGAAGAAGCTAATGAGAGATGAAGAGATAAACCAACTAGAAAGTGTTGTTGTGGCAAAGATGGGAAACTTGGATAGGCTTTGTGATGCTTTTAGGGGCTGCCATGCAATTTTTCATACTTCTTCCTTCATTGATCCACATGGTGTCTCTGGTTATTCTGGACAAATGGCATTTCTTGAAACTGAAGGGGCAAAGAATGTGATCGAAGCTTGTGGCAGGGCAGCGCATGCAAAGAGATGCATCTTCACTTCCTCTCTTCTTGCTTCTATTTGGACAGGTGATAGTGAAAACAAGATCATTGATGAGTTTAGCTGGAGCAATGAAGAATTCTGCAGGGAAAACAAGCTTTGGCTGGCCTTGGGGAAGACCACTGCAGAGATGGCTTGTTGGAGAAAGTCAAAGGAAATGAAAGTCAATCTTGTGACAGTTTGCCCCGGCCTTCTCATGGACTCATATGCTCACAAGGACAGCTCTATACCCTATCTTAAAGGTGGCAAGGACATGCTAAAAAATGGTCACCTAGCCATTGAGGATGTGAAGAAGGCTGCAGAGGCGCACGTCTATGTATATGAGGCCATGGATAGTGGAGCATGTGGGAGATACCTGTGCTTTGGGAAGGTTGTAAGAACAAGAAGTGAAGCCCTTGAACTAGAAAACATGTTAAGGGTTCAGGGTTTGTTATCAGGAGGAAGCCTTGAAGTTTTTGACGAAGAAACAGGTGCAGAAATAAACAGCATGCTTAGTAATGCAAAGCTGGTCAGATTGATTCAAGCTTCTCAAAGATTATCGTGCAAGCCAGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD NAD_binding_10

Protein Analysis

345

Amino Acids

38.6

Weight (kDa)

6.18

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 41 - 222 2.5e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 43 - 191 1.2e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 49 - 167 1.7e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016903)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45521
malus_domestica MD09G1082100.v1.1
prunus_persica Prupe.3G241700_v2.0.a1
pyrus_communis pycom09g00860
rosa_chinensis RchiOBHm_Chr2g0163471
rosa_laevigata RLG00000021404
rosa_multiflora Rmu_co8460151.1_g000001
rosa_roxburghii Rroxscaffold_2G00087290
rosa_rugosa Rorug02G0503000
rosa_samantha Rh2AG570000 Rh2BG582200 Rh2CG552000 Rh2DG592000
rosa_wichuraiana Rw2G047190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 2 cut(s) 139, 557
AcuI CTGAAG 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 6 cut(s) 105, 209, 410, 878, 929, 1001
AjiI CACGTC 1 cut(s) 790
AjnI CCWGG 1 cut(s) 147
AluBI AGCT 7 cut(s) 241, 440, 543, 577, 702, 985, 1004
AluI AGCT 7 cut(s) 241, 440, 543, 577, 702, 985, 1004
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 359
AoxI GGCC 3 cut(s) 585, 667, 804
ApeKI GCWGC 4 cut(s) 22, 330, 452, 776
ApoI RAATTY 2 cut(s) 139, 557
AspLEI GCGC 2 cut(s) 457, 787
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 3 cut(s) 193, 521, 740
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 234, 602
BbvI GCAGC 4 cut(s) 9, 317, 464, 763
BccI CCATC 2 cut(s) 286, 604
BciT130I CCWGG 1 cut(s) 149
BcnI CCSGG 1 cut(s) 666
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 3 cut(s) 269, 752, 881
BfmI CTRYAG 3 cut(s) 562, 603, 777
BisI GCNGC 4 cut(s) 23, 331, 453, 777
BlsI GCNGC 4 cut(s) 24, 332, 454, 778
Bme1390I CCNGG 2 cut(s) 149, 666
BmgBI CACGTC 1 cut(s) 790
BmrFI CCNGG 2 cut(s) 149, 666
BmsI GCATC 3 cut(s) 307, 458, 480
BpiI GAAGAC 2 cut(s) 234, 602
BpmI CTGGAG 1 cut(s) 565
BpuMI CCSGG 1 cut(s) 666
BsaJI CCNNGG 4 cut(s) 148, 588, 664, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 132, 1028
Bse3DI GCAATG 2 cut(s) 12, 556
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 4 cut(s) 148, 588, 664, 807
BseGI GGATG 1 cut(s) 769
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 2 cut(s) 12, 556
BseNI ACTGG 2 cut(s) 132, 1028
BseXI GCAGC 4 cut(s) 9, 317, 464, 763
BsgI GTGCAG 2 cut(s) 109, 972
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 3 cut(s) 587, 669, 806
BsiSI CCGG 1 cut(s) 666
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 3 cut(s) 587, 669, 806
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 39, 364, 432, 525
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 1 cut(s) 183
BspANI GGCC 3 cut(s) 587, 669, 806
BspFNI CGCG 1 cut(s) 183
BspMAI CTGCAG 3 cut(s) 566, 607, 781
BspPI GGATC 1 cut(s) 359
BsrDI GCAATG 2 cut(s) 12, 556
BsrI ACTGG 2 cut(s) 132, 1028
BssECI CCNNGG 4 cut(s) 148, 588, 664, 807
BssMI GATC 4 cut(s) 39, 364, 432, 525
BssT1I CCWWGG 2 cut(s) 588, 807
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 2 cut(s) 249, 492
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 181, 459, 585, 968, 1025
BstDEI CTNAG 1 cut(s) 971
BstDSI CCRYGG 1 cut(s) 807
BstEII GGTNACC 1 cut(s) 746
BstF5I GGATG 1 cut(s) 769
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 2 cut(s) 457, 787
BstKTI GATC 4 cut(s) 42, 367, 435, 528
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 4 cut(s) 39, 364, 432, 525
BstMWI GCNNNNNNNGC 5 cut(s) 28, 176, 458, 611, 782
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 5 cut(s) 461, 736, 825, 892, 970
BstPI GGTNACC 1 cut(s) 746
BstSCI CCNGG 2 cut(s) 147, 664
BstSFI CTRYAG 3 cut(s) 562, 603, 777
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 4 cut(s) 9, 317, 464, 763
BstV2I GAAGAC 2 cut(s) 234, 602
BsuRI GGCC 3 cut(s) 587, 669, 806
BtgI CCRYGG 1 cut(s) 807
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 1 cut(s) 769
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 139, 600
Cac8I GCNNGC 5 cut(s) 181, 459, 585, 968, 1025
CfoI GCGC 2 cut(s) 457, 787
DdeI CTNAG 1 cut(s) 971
DpnI GATC 4 cut(s) 41, 366, 434, 527
DpnII GATC 4 cut(s) 39, 364, 432, 525
DraIII CACNNNGTG 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 249, 492
EarI CTCTTC 2 cut(s) 249, 492
Eco130I CCWWGG 2 cut(s) 588, 807
Eco24I GRGCYC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 746
EcoO65I GGTNACC 1 cut(s) 746
EcoRI GAATTC 2 cut(s) 139, 557
EcoRII CCWGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 2 cut(s) 588, 807
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 588, 807
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FauNDI CATATG 1 cut(s) 685
Fnu4HI GCNGC 4 cut(s) 23, 331, 453, 777
FokI GGATG 1 cut(s) 776
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 4 cut(s) 23, 331, 453, 777
FspBI CTAG 3 cut(s) 269, 752, 881
GlaI GCGC 2 cut(s) 456, 786
GluI GCNGC 4 cut(s) 23, 331, 453, 777
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 3 cut(s) 587, 669, 806
HapII CCGG 1 cut(s) 666
HhaI GCGC 2 cut(s) 457, 787
Hin6I GCGC 2 cut(s) 455, 785
HinP1I GCGC 2 cut(s) 455, 785
HindIII AAGCTT 3 cut(s) 438, 575, 1002
HinfI GANTC 5 cut(s) 115, 193, 198, 680, 997
HpaII CCGG 1 cut(s) 666
HphI GGTGA 3 cut(s) 193, 521, 740
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 192, 992
Hpy188III TCNNGA 4 cut(s) 105, 390, 407, 915
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 5 cut(s) 367, 410, 680, 766, 841
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 1 cut(s) 789
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 176, 458, 611, 782
HpyF3I CTNAG 1 cut(s) 971
HpySE526I ACGT 1 cut(s) 789
HspAI GCGC 2 cut(s) 455, 785
Kzo9I GATC 4 cut(s) 39, 364, 432, 525
LmnI GCTCC 2 cut(s) 546, 818
Lsp1109I GCAGC 4 cut(s) 9, 317, 464, 763
LweI GCATC 3 cut(s) 307, 458, 480
MaeI CTAG 3 cut(s) 269, 752, 881
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 4 cut(s) 127, 199, 652, 746
MalI GATC 4 cut(s) 41, 366, 434, 527
MboI GATC 4 cut(s) 39, 364, 432, 525
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 3 cut(s) 139, 339, 557
MlyI GAGTC 2 cut(s) 207, 674
MmeI TCCRAC 1 cut(s) 599
MnlI CCTC 7 cut(s) 43, 211, 493, 754, 775, 796, 911
Mph1103I ATGCAT 1 cut(s) 473
MseI TTAA 2 cut(s) 717, 893
MslI CAYNNNNRTG 2 cut(s) 85, 792
MspI CCGG 1 cut(s) 666
MspR9I CCNGG 2 cut(s) 149, 666
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 5 cut(s) 28, 176, 458, 611, 782
NciI CCSGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 807
NdeI CATATG 1 cut(s) 685
NdeII GATC 4 cut(s) 39, 364, 432, 525
NmuCI GTSAC 4 cut(s) 127, 199, 652, 746
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 5 cut(s) 461, 736, 825, 892, 970
PaeI GCATGC 2 cut(s) 461, 970
PciI ACATGT 1 cut(s) 888
PctI GAATGC 1 cut(s) 80
PfeI GAWTC 3 cut(s) 115, 193, 997
PkrI GCNGC 4 cut(s) 24, 332, 454, 778
PleI GAGTC 2 cut(s) 206, 674
PpsI GAGTC 2 cut(s) 206, 674
PscI ACATGT 1 cut(s) 888
Psp6I CCWGG 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 746
PspGI CCWGG 1 cut(s) 147
PstI CTGCAG 3 cut(s) 566, 607, 781
RseI CAYNNNNRTG 2 cut(s) 85, 792
SaqAI TTAA 2 cut(s) 717, 893
SatI GCNGC 4 cut(s) 23, 331, 453, 777
Sau3AI GATC 4 cut(s) 39, 364, 432, 525
SchI GAGTC 2 cut(s) 207, 674
ScrFI CCNGG 2 cut(s) 149, 666
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 307, 458, 480
SfcI CTRYAG 3 cut(s) 562, 603, 777
SmiMI CAYNNNNRTG 2 cut(s) 85, 792
SphI GCATGC 2 cut(s) 461, 970
Sse9I AATT 3 cut(s) 139, 339, 557
SsiI CCGC 1 cut(s) 183
SspMI CTAG 3 cut(s) 269, 752, 881
StyD4I CCNGG 2 cut(s) 147, 664
StyI CCWWGG 2 cut(s) 588, 807
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 1 cut(s) 792
TaqI TCGA 2 cut(s) 196, 435
TasI AATT 3 cut(s) 139, 339, 557
TfiI GAWTC 3 cut(s) 115, 193, 997
Tru1I TTAA 2 cut(s) 717, 893
Tru9I TTAA 2 cut(s) 717, 893
TscAI CASTG 2 cut(s) 139, 607
TseFI GTSAC 4 cut(s) 127, 199, 652, 746
TseI GCWGC 4 cut(s) 22, 330, 452, 776
Tsp45I GTSAC 4 cut(s) 127, 199, 652, 746
TspDTI ATGAA 7 cut(s) 240, 248, 267, 335, 349, 567, 653
TspRI CASTG 2 cut(s) 139, 607
XapI RAATTY 2 cut(s) 139, 557
XceI RCATGY 5 cut(s) 461, 736, 825, 892, 970
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XspI CTAG 3 cut(s) 269, 752, 881
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.