RchiOBHm_Chr1g0313141
ERF Family

Major facilitator superfamily domain-containing protein 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005390
Physical Location & Seq
Reverse (-)
258050 .. 270584
12535 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGCATAATGTTACTGAAGATGATGATGATGATTCAGTCACAAAGCCCCTTGGAAGGTGTTCTGTCATGTATTATGGCGTGGGCCATATGCTCAATGATATCACTGCTTCCTGTTGGTTTACATATCTCTTGTTATTCTTGACAGATATTGGACTATCCCCCAGGGATGCTGCCACTGTAATGCTATCTGGTCAAATAGCTGATGGATTTGCAACCATATTTGCTGGCGAGCTGATAGACAGATTTGGACATTTCAAATTGTGGCATGGTGCAGGCTCTATTTTGGTTGCCGTTTCATTTTCTTCTGTTTTTGGTGGTTGTGTACCGTGTAAAATTTTTGGTACCTCTTCACCCGCATTGCGAACTGTTGGTTACAGCATTTTTGCTGCTATCTTTAATGTGGGTTGGGCAGCTACCCAGGTCTCACACATGTCTATGCTGAATTGCATTACGCTGAATTCAACAAGCAGAGTGGTGCTCGCTAGCTGTCGGAATGCTTTTACTATGGTTGCCAACCTGAGCTTGTATGCGGTTGCTTTTATAATCTTTGCTGTCACGATAGCAAGAACACATGCCGATATTGAAAATCAGTACCGCTGGATTGCATATTTGTCAATCTTCATAGGATGCTGCTTTGTTGGTATCTTTCAACTCGGCACCAAAGAGCCAAGATTAAAGGTAGGTGTACAAGGAAGTAATTCTTCAAGGATTCCATGGACGTACTGGTTCAAGAGAGTGCTATATTATCAGGTTGCTCTTGTTTATGTGCTGACCAGACTAGTGGTTAATGTTTCACAGGCATATCTTGCATTCTATGTTATAGATGATCTGCAGATGGCACAATCGGCTAAAGCTCTGGTTCCTGCGATCATCTACATAAGTAGCTTCCTCGTATCTGTGATATTGCAGGAGATACAATGGACTGGCCGATTCTTGAAGACCTACTACTCAGCTGGAGCTATTATTTGGATATTTTGTGGTGCAGGGATCCTGCTTTTACCTAGAAGCTTGAGTGCTTTCATGTACATTATATCTATATTTATTGGCATAGCAAATGCTTTAATGATGGTGACCGGAGTAAGCATGCAAAGTGTTTTAATTGATACAGATCTCAATGGCTGTGCATTTGTTTGTGGATCATTCAGCTTCTTGGACAAAATCTCATGTGGGCTTGCTTTATTTGTTCTTCAGTCGTATCAAAGTGATTCAGTCATACAGAATATCCATTCTAATCATGTCTACATATCAGTTACGAGATTCGGGTTGGGTCTTGTTCCGGCATTTTGTTCACTTGTATCAGTGGCTGTTACATACTCGATGAACCTCCACTCTGCTCGCTGCAAACCTCTAATGGAGCCTCTATTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_2

Protein Analysis

456

Amino Acids

50.09

Weight (kDa)

8.14

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_2 PF13347 25 - 441 3.4e-14 MFS/sugar transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 2 cut(s) 341, 656
AccI GTMKAC 1 cut(s) 1239
AciI CCGC 3 cut(s) 354, 530, 595
AclWI GGATC 3 cut(s) 982, 995, 1144
AcoI YGGCCR 1 cut(s) 925
AcsI RAATTY 2 cut(s) 333, 457
AcuI CTGAAG 2 cut(s) 36, 1172
AfaI GTAC 6 cut(s) 324, 343, 593, 687, 722, 1025
AfiI CCNNNNNNNGG 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 7 cut(s) 256, 462, 584, 650, 705, 730, 937
AhlI ACTAGT 1 cut(s) 778
AjnI CCWGG 2 cut(s) 161, 417
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 427
AlwI GGATC 3 cut(s) 982, 995, 1144
AlwNI CAGNNNCTG 1 cut(s) 1304
AoxI GGCC 2 cut(s) 82, 925
ApeKI GCWGC 5 cut(s) 170, 386, 410, 630, 1338
ApoI RAATTY 2 cut(s) 333, 457
Asp700I GAANNNNTTC 2 cut(s) 58, 697
Asp718I GGTACC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 342, 1081
AsuNHI GCTAGC 1 cut(s) 482
BamHI GGATCC 1 cut(s) 987
BanI GGYRCC 2 cut(s) 341, 656
BarI GAAGNNNNNNTAC 2 cut(s) 929, 961
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 5 cut(s) 157, 373, 422, 617, 1325
BccI CCATC 3 cut(s) 197, 829, 1060
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 2 cut(s) 163, 419
BcoDI GTCTC 1 cut(s) 427
BcuI ACTAGT 1 cut(s) 778
BfaI CTAG 3 cut(s) 483, 779, 1002
BfmI CTRYAG 1 cut(s) 830
BglII AGATCT 1 cut(s) 1108
BisI GCNGC 5 cut(s) 171, 387, 411, 631, 1339
BlsI GCNGC 5 cut(s) 172, 388, 412, 632, 1340
Bme1390I CCNGG 2 cut(s) 163, 419
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 5 cut(s) 343, 658, 861, 989, 1356
BmrFI CCNGG 2 cut(s) 163, 419
BmsI GCATC 2 cut(s) 157, 617
BmtI GCTAGC 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 944
BplI GAGNNNNNCTC 2 cut(s) 462, 494
BpmI CTGGAG 1 cut(s) 975
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 1107
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 5 cut(s) 49, 161, 162, 417, 713
BsaWI WCCGGW 1 cut(s) 1073
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse1I ACTGG 2 cut(s) 728, 928
Bse3DI GCAATG 1 cut(s) 356
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 163, 419
BseDI CCNNGG 5 cut(s) 49, 161, 162, 417, 713
BseGI GGATG 2 cut(s) 172, 632
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 509, 963
BseNI ACTGG 2 cut(s) 728, 928
BseXI GCAGC 5 cut(s) 157, 373, 422, 617, 1325
BsgI GTGCAG 2 cut(s) 291, 1002
BshFI GGCC 2 cut(s) 84, 927
BshNI GGYRCC 2 cut(s) 341, 656
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 2 cut(s) 1074, 1277
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 427
BsmI GAATGC 2 cut(s) 499, 809
BsnI GGCC 2 cut(s) 84, 927
Bso31I GGTCTC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 480
Bsp1407I TGTACA 2 cut(s) 685, 1023
Bsp143I GATC 5 cut(s) 826, 867, 987, 1108, 1136
Bsp19I CCATGG 1 cut(s) 713
BspACI CCGC 3 cut(s) 354, 530, 595
BspANI GGCC 2 cut(s) 84, 927
BspCNI CTCAG 2 cut(s) 510, 962
BspLI GGNNCC 5 cut(s) 343, 658, 861, 989, 1356
BspMAI CTGCAG 1 cut(s) 834
BspOI GCTAGC 1 cut(s) 486
BspPI GGATC 3 cut(s) 982, 995, 1144
BspT107I GGYRCC 2 cut(s) 341, 656
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 356
BsrGI TGTACA 2 cut(s) 685, 1023
BsrI ACTGG 2 cut(s) 728, 928
BssECI CCNNGG 5 cut(s) 49, 161, 162, 417, 713
BssMI GATC 5 cut(s) 826, 867, 987, 1108, 1136
BssT1I CCWWGG 2 cut(s) 49, 713
Bst2UI CCWGG 2 cut(s) 163, 419
Bst4CI ACNGT 3 cut(s) 178, 327, 367
Bst6I CTCTTC 1 cut(s) 352
BstAPI GCANNNNNTGC 1 cut(s) 806
BstAUI TGTACA 2 cut(s) 685, 1023
BstC8I GCNNGC 8 cut(s) 226, 230, 274, 480, 484, 1085, 1173, 1336
BstDEI CTNAG 2 cut(s) 518, 949
BstDSI CCRYGG 1 cut(s) 713
BstEII GGTNACC 1 cut(s) 1069
BstF5I GGATG 2 cut(s) 172, 632
BstKTI GATC 5 cut(s) 829, 870, 990, 1111, 1139
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 5 cut(s) 826, 867, 987, 1108, 1136
BstMWI GCNNNNNNNGC 2 cut(s) 806, 845
BstNI CCWGG 2 cut(s) 163, 419
BstNSI RCATGY 3 cut(s) 433, 575, 1087
BstPI GGTNACC 1 cut(s) 1069
BstSCI CCNGG 2 cut(s) 161, 417
BstSFI CTRYAG 1 cut(s) 830
BstV1I GCAGC 5 cut(s) 157, 373, 422, 617, 1325
BstV2I GAAGAC 1 cut(s) 944
BstX2I RGATCY 2 cut(s) 987, 1108
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 2 cut(s) 987, 1108
BsuRI GGCC 2 cut(s) 84, 927
BtgI CCRYGG 1 cut(s) 713
BtsCI GGATG 2 cut(s) 172, 632
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 3 cut(s) 102, 174, 1305
Cac8I GCNNGC 8 cut(s) 226, 230, 274, 480, 484, 1085, 1173, 1336
CaiI CAGNNNCTG 1 cut(s) 1304
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 6 cut(s) 323, 342, 592, 686, 721, 1024
CspCI CAANNNNNGTGG 2 cut(s) 453, 488
CviAII CATG 9 cut(s) 67, 266, 430, 572, 714, 1021, 1084, 1164, 1235
CviQI GTAC 6 cut(s) 323, 342, 592, 686, 721, 1024
DdeI CTNAG 2 cut(s) 518, 949
DpnI GATC 5 cut(s) 828, 869, 989, 1110, 1138
DpnII GATC 5 cut(s) 826, 867, 987, 1108, 1136
EaeI YGGCCR 1 cut(s) 925
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 49, 713
Eco31I GGTCTC 1 cut(s) 427
Eco32I GATATC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 36, 1172
Eco91I GGTNACC 1 cut(s) 1069
EcoO65I GGTNACC 1 cut(s) 1069
EcoRI GAATTC 1 cut(s) 457
EcoRII CCWGG 2 cut(s) 161, 417
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 49, 713
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 49, 713
FaeI CATG 9 cut(s) 70, 269, 433, 575, 717, 1024, 1087, 1167, 1238
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FatI CATG 9 cut(s) 66, 265, 429, 571, 713, 1020, 1083, 1163, 1234
FauI CCCGC 1 cut(s) 361
FauNDI CATATG 1 cut(s) 87
FblI GTMKAC 1 cut(s) 1239
Fnu4HI GCNGC 5 cut(s) 171, 387, 411, 631, 1339
FokI GGATG 2 cut(s) 179, 639
Fsp4HI GCNGC 5 cut(s) 171, 387, 411, 631, 1339
FspBI CTAG 3 cut(s) 483, 779, 1002
GluI GCNGC 5 cut(s) 171, 387, 411, 631, 1339
GsuI CTGGAG 1 cut(s) 975
HaeIII GGCC 2 cut(s) 84, 927
HapII CCGG 2 cut(s) 1074, 1277
Hin1II CATG 9 cut(s) 70, 269, 433, 575, 717, 1024, 1087, 1167, 1238
HindIII AAGCTT 1 cut(s) 1006
HinfI GANTC 5 cut(s) 32, 709, 930, 1205, 1257
HpaII CCGG 2 cut(s) 1074, 1277
HphI GGTGA 2 cut(s) 342, 1081
Hpy166II GTNNAC 5 cut(s) 120, 323, 686, 1240, 1289
Hpy188I TCNGA 1 cut(s) 492
Hpy188III TCNNGA 4 cut(s) 139, 556, 730, 934
Hpy8I GTNNAC 5 cut(s) 120, 323, 686, 1240, 1289
HpyAV CCTTC 1 cut(s) 48
HpyCH4III ACNGT 3 cut(s) 178, 327, 367
HpyCH4IV ACGT 1 cut(s) 719
HpyF10VI GCNNNNNNNGC 2 cut(s) 806, 845
HpyF3I CTNAG 2 cut(s) 518, 949
HpySE526I ACGT 1 cut(s) 719
Hsp92II CATG 9 cut(s) 70, 269, 433, 575, 717, 1024, 1087, 1167, 1238
KpnI GGTACC 1 cut(s) 345
Kzo9I GATC 5 cut(s) 826, 867, 987, 1108, 1136
LmnI GCTCC 2 cut(s) 956, 1354
Lsp1109I GCAGC 5 cut(s) 157, 373, 422, 617, 1325
LweI GCATC 2 cut(s) 157, 617
MaeI CTAG 3 cut(s) 483, 779, 1002
MaeII ACGT 1 cut(s) 719
MaeIII GTNAC 7 cut(s) 10, 37, 371, 553, 1069, 1249, 1306
MalI GATC 5 cut(s) 828, 869, 989, 1110, 1138
MboI GATC 5 cut(s) 826, 867, 987, 1108, 1136
MboII GAAGA 7 cut(s) 29, 294, 339, 610, 693, 949, 1178
MflI RGATCY 2 cut(s) 987, 1108
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 6 cut(s) 257, 333, 442, 457, 697, 1098
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 5 cut(s) 355, 899, 1334, 1356, 1368
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 58, 697
MseI TTAA 5 cut(s) 396, 674, 786, 1061, 1097
MslI CAYNNNNRTG 2 cut(s) 179, 434
MspA1I CMGCKG 2 cut(s) 597, 953
MspI CCGG 2 cut(s) 1074, 1277
MspR9I CCNGG 2 cut(s) 163, 419
Mva1269I GAATGC 2 cut(s) 499, 809
MvaI CCWGG 2 cut(s) 163, 419
MwoI GCNNNNNNNGC 2 cut(s) 806, 845
NcoI CCATGG 1 cut(s) 713
NdeI CATATG 1 cut(s) 87
NdeII GATC 5 cut(s) 826, 867, 987, 1108, 1136
NheI GCTAGC 1 cut(s) 482
NlaIII CATG 9 cut(s) 70, 269, 433, 575, 717, 1024, 1087, 1167, 1238
NlaIV GGNNCC 5 cut(s) 343, 658, 861, 989, 1356
NmeAIII GCCGAG 1 cut(s) 633
NmuCI GTSAC 3 cut(s) 37, 553, 1069
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 433, 575, 1087
PaeI GCATGC 1 cut(s) 1087
PasI CCCWGGG 1 cut(s) 162
PciI ACATGT 1 cut(s) 429
PctI GAATGC 2 cut(s) 499, 809
PdmI GAANNNNTTC 2 cut(s) 58, 697
PfeI GAWTC 5 cut(s) 32, 709, 930, 1205, 1257
PkrI GCNGC 5 cut(s) 172, 388, 412, 632, 1340
PscI ACATGT 1 cut(s) 429
PsiI TTATAA 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 161, 417
PspEI GGTNACC 1 cut(s) 1069
PspGI CCWGG 2 cut(s) 161, 417
PspN4I GGNNCC 5 cut(s) 343, 658, 861, 989, 1356
PspPI GGNCC 1 cut(s) 82
PstI CTGCAG 1 cut(s) 834
PstNI CAGNNNCTG 1 cut(s) 1304
PsuI RGATCY 2 cut(s) 987, 1108
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 6 cut(s) 324, 343, 593, 687, 722, 1025
RsaNI GTAC 6 cut(s) 323, 342, 592, 686, 721, 1024
RseI CAYNNNNRTG 2 cut(s) 179, 434
SaqAI TTAA 5 cut(s) 396, 674, 786, 1061, 1097
SatI GCNGC 5 cut(s) 171, 387, 411, 631, 1339
Sau3AI GATC 5 cut(s) 826, 867, 987, 1108, 1136
Sau96I GGNCC 1 cut(s) 82
ScrFI CCNGG 2 cut(s) 163, 419
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 2 cut(s) 157, 617
SfcI CTRYAG 1 cut(s) 830
SmiMI CAYNNNNRTG 2 cut(s) 179, 434
SmlI CTYRAG 1 cut(s) 1009
SmoI CTYRAG 1 cut(s) 1009
SpeI ACTAGT 1 cut(s) 778
SphI GCATGC 1 cut(s) 1087
Sse9I AATT 6 cut(s) 257, 333, 442, 457, 697, 1098
SsiI CCGC 3 cut(s) 354, 530, 595
SspMI CTAG 3 cut(s) 483, 779, 1002
StyD4I CCNGG 2 cut(s) 161, 417
StyI CCWWGG 2 cut(s) 49, 713
TaaI ACNGT 3 cut(s) 178, 327, 367
TaiI ACGT 1 cut(s) 722
TaqI TCGA 1 cut(s) 1316
TasI AATT 6 cut(s) 257, 333, 442, 457, 697, 1098
TatI WGTACW 2 cut(s) 685, 1023
TfiI GAWTC 5 cut(s) 32, 709, 930, 1205, 1257
Tru1I TTAA 5 cut(s) 396, 674, 786, 1061, 1097
Tru9I TTAA 5 cut(s) 396, 674, 786, 1061, 1097
TscAI CASTG 3 cut(s) 109, 181, 1305
TseFI GTSAC 3 cut(s) 37, 553, 1069
TseI GCWGC 5 cut(s) 170, 386, 410, 630, 1338
Tsp45I GTSAC 3 cut(s) 37, 553, 1069
TspDTI ATGAA 4 cut(s) 285, 610, 1009, 1334
TspRI CASTG 3 cut(s) 109, 181, 1305
XapI RAATTY 2 cut(s) 333, 457
XceI RCATGY 3 cut(s) 433, 575, 1087
XcmI CCANNNNNNNNNTGG 1 cut(s) 720
XmiI GTMKAC 1 cut(s) 1239
XmnI GAANNNNTTC 2 cut(s) 58, 697
XspI CTAG 3 cut(s) 483, 779, 1002
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.