RchiOBHm_Chr1g0318331

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0007095
Physical Location & Seq
Forward (+)
5830141 .. 5834202
4062 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1509 bp
ATGAATATTCCCAAGCCTTTTTTGGTTCTTTTCTCTTCCTTTCTCATTCTTTCTTGCTTCTCCATAGCTCAAATCCATGGGAGCAAGCATTCACAAGCGCTTTCTCAGCTGTACAAGTCCAAGCTGAATTCCAAAAGCGCAGGCATCGACACGAAGCCATTCGAGGCGATTCTTCATGCGAACGCAACCAGCATTCATCCCCAAGAAGGGTTGAAGGAGAAAGATCGGATCAAAAGCTTGCCTGGACAACCCAAGGTGAAGTTCAGTCAGTATGGTGGGTATGTTACTGTGGATAAAAAAGCAGGTCGTGCACTTTTTTACTACTTTGCTGAAGCTGACAATCAGCAGGCCAAGGATTCTTTGCCTCTTCTTCTATGGCTTAATGGAGGACCTGGATGTTCCTCTCTTGCCTATGGAGCAATGCTTGAGCTCGGACCATTCCGTATCCATAGCGATGGCAAAACCCTTTACAGAAATAGATTTTCATGGAACTACGCTGCAAATGTTTTGTTCCTTGAGTCTCCTGCTGGTGTGGGTTTTTCATACTCTAACAGAACATCAGATTATGATAAAAGTGGGGACAGAAGAACAGCTGCAGATAATTATGTGTTCTTAGTGAATTGGTTGGAGAGGTTTCCTGAATATAAGGGGAGGGAGTTCTATATTGCTGGTGAAAGCTATGCAGGGCACTATGTACCTCAACTTGCACAGACTATCCTCCACCACAACAAGAAGGCTAATAGAACCATCATCAACCTCAAGGGCATAATTATTGGGAATGCAGTGATTAATGATGAAACTGATACAAGAGGAATGTATGACTACCTTGCTTCCCATGCTCTCATTTCCGATGAAATTGCAAATCAAGTCCGAAAATACTGTGATTTTTCACCCAATGCCACTATTCAACCCAAAGAGTGCGATGACGCTACTGTTGCTATTGAGAAGGCTACCTATTTCATTGATATCTATAACATCTATGCCCCCTCATGTGCCTCTTCCAATCTCACTGTCCAACCCAAAAAGGCTTCAATACTGAAATTTGATCCGTGCAGCGAGTACTATGCGGAAGCTTATTTGAATCGGCCTGATGTTCAAGAAGCTCTCCATGCTAATGTCACTAAACTCACACATGACTGGGAACCATGTAGTGATGTAATCAAACACTGGAGAGATAGCTCATCAACTATACTACCCCTTCTACATGAGTTTATGGATAATGGACTTCGAGTCTGGATTTTTAGTGGCGATATCGATGGAAGGGTTCCAGTCACTTCAACAAAGTATTCTCTTGAGAAGATGAATCTACCAATCAAGACCGAATGGCATGCTTGGTTCCTTAGCGGACAAGTTGGTGGTTACACACAAGTGTATGAAGGAGACATAACACTTGCCACAGTGAGGGAGGCAGGGCATCAAGTCCCAAGCTACCAGCCTGCTAGGGCACTTTCACTCATCAAGCACTTCCTTGATGGCACACCTCTTCCTGATACTAAAAGATATAATTAA

Protein Analysis

502

Amino Acids

56.44

Weight (kDa)

7.64

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 81 - 492 4.6e-143 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 293
AciI CCGC 2 cut(s) 1067, 1344
AclWI GGATC 2 cut(s) 236, 1040
AcsI RAATTY 2 cut(s) 127, 1040
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 3 cut(s) 113, 696, 1061
AfeI AGCGCT 1 cut(s) 99
AfiI CCNNNNNNNGG 3 cut(s) 206, 207, 1401
AgsI TTSAA 6 cut(s) 214, 908, 1032, 1081, 1097, 1278
AhdI GACNNNNNGTC 1 cut(s) 1229
AjnI CCWGG 2 cut(s) 241, 391
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AleI CACNNNNGTG 1 cut(s) 1367
Alw21I GWGCWC 2 cut(s) 313, 432
Alw26I GTCTC 2 cut(s) 525, 1374
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 2 cut(s) 236, 1040
Aor51HI AGCGCT 1 cut(s) 99
AoxI GGCC 2 cut(s) 348, 1085
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 497, 593, 1053
ApoI RAATTY 2 cut(s) 127, 1040
AseI ATTAAT 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 2 cut(s) 100, 140
AspS9I GGNCC 2 cut(s) 389, 434
AsuHPI GGTGA 3 cut(s) 268, 683, 882
AvaII GGWCC 2 cut(s) 389, 434
BaeGI GKGCMC 3 cut(s) 313, 690, 1447
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 313, 432
BbvI GCAGC 3 cut(s) 484, 580, 1065
BccI CCATC 4 cut(s) 449, 755, 1250, 1466
BcgI CGANNNNNNTGC 6 cut(s) 839, 873, 1046, 1080, 1310, 1344
BciT130I CCWGG 2 cut(s) 243, 393
BciVI GTATCC 1 cut(s) 455
BcoDI GTCTC 2 cut(s) 525, 1374
BfaI CTAG 1 cut(s) 1440
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 455
BisI GCNGC 3 cut(s) 498, 594, 1054
BlsI GCNGC 3 cut(s) 499, 595, 1055
BmcAI AGTACT 1 cut(s) 1061
Bme1390I CCNGG 2 cut(s) 243, 393
Bme18I GGWCC 2 cut(s) 389, 434
BmeRI GACNNNNNGTC 1 cut(s) 1229
BmgT120I GGNCC 2 cut(s) 389, 434
BmiI GGNNCC 3 cut(s) 1143, 1266, 1337
BmrFI CCNGG 2 cut(s) 243, 393
BmrI ACTGGG 1 cut(s) 1147
BmsI GCATC 2 cut(s) 153, 1423
BmuI ACTGGG 1 cut(s) 1147
BpmI CTGGAG 1 cut(s) 1189
Bpu10I CCTNAGC 1 cut(s) 1340
BpuEI CTTGAG 4 cut(s) 446, 536, 743, 1313
Bsa29I ATCGAT 1 cut(s) 1254
BsaJI CCNNGG 3 cut(s) 76, 252, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 207, 1401
Bse1I ACTGG 3 cut(s) 1142, 1172, 1268
Bse3DI GCAATG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 243, 393
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 3 cut(s) 76, 252, 351
BseGI GGATG 2 cut(s) 196, 401
BseLI CCNNNNNNNGG 3 cut(s) 206, 207, 1401
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 3 cut(s) 1142, 1172, 1268
BseSI GKGCMC 3 cut(s) 313, 690, 1447
BseXI GCAGC 3 cut(s) 484, 580, 1065
BsgI GTGCAG 1 cut(s) 1072
BshFI GGCC 2 cut(s) 350, 1087
BshVI ATCGAT 1 cut(s) 1254
BsiHKAI GWGCWC 2 cut(s) 313, 432
BslFI GGGAC 2 cut(s) 593, 1406
BslI CCNNNNNNNGG 3 cut(s) 206, 207, 1401
BsmAI GTCTC 2 cut(s) 525, 1374
BsmFI GGGAC 2 cut(s) 593, 1406
BsmI GAATGC 3 cut(s) 88, 192, 784
BsnI GGCC 2 cut(s) 350, 1087
Bsp1286I GDGCHC 4 cut(s) 313, 432, 690, 1447
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 3 cut(s) 223, 228, 1045
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 2 cut(s) 1067, 1344
BspANI GGCC 2 cut(s) 350, 1087
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 1254
BspLI GGNNCC 3 cut(s) 1143, 1266, 1337
BspMAI CTGCAG 1 cut(s) 598
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 2 cut(s) 236, 1040
BsrDI GCAATG 1 cut(s) 426
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 1142, 1172, 1268
BssECI CCNNGG 3 cut(s) 76, 252, 351
BssMI GATC 3 cut(s) 223, 228, 1045
BssT1I CCWWGG 3 cut(s) 76, 252, 351
Bst2UI CCWGG 2 cut(s) 243, 393
Bst4CI ACNGT 5 cut(s) 289, 881, 934, 1012, 1399
Bst6I CTCTTC 4 cut(s) 40, 372, 1003, 1488
BstAPI GCANNNNNTGC 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 6 cut(s) 86, 142, 239, 348, 1329, 1437
BstDEI CTNAG 3 cut(s) 105, 613, 1340
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 2 cut(s) 196, 401
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 2 cut(s) 100, 140
BstKTI GATC 3 cut(s) 226, 231, 1048
BstMAI GTCTC 2 cut(s) 525, 1374
BstMBI GATC 3 cut(s) 223, 228, 1045
BstMWI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
BstNI CCWGG 2 cut(s) 243, 393
BstNSI RCATGY 1 cut(s) 1331
BstSCI CCNGG 2 cut(s) 241, 391
BstSFI CTRYAG 1 cut(s) 594
BstSLI GKGCMC 3 cut(s) 313, 690, 1447
BstV1I GCAGC 3 cut(s) 484, 580, 1065
BstXI CCANNNNNNTGG 1 cut(s) 455
Bsu15I ATCGAT 1 cut(s) 1254
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 2 cut(s) 350, 1087
BsuTUI ATCGAT 1 cut(s) 1254
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 2 cut(s) 468, 936
BtsCI GGATG 2 cut(s) 196, 401
BtsI GCAGTG 1 cut(s) 789
BtsIMutI CAGTG 4 cut(s) 789, 1008, 1165, 1404
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 6 cut(s) 86, 142, 239, 348, 1329, 1437
CfoI GCGC 2 cut(s) 100, 140
Cfr13I GGNCC 2 cut(s) 389, 434
ClaI ATCGAT 1 cut(s) 1254
CseI GACGC 1 cut(s) 935
Csp6I GTAC 3 cut(s) 112, 695, 1060
CviQI GTAC 3 cut(s) 112, 695, 1060
DdeI CTNAG 3 cut(s) 105, 613, 1340
DpnI GATC 3 cut(s) 225, 230, 1047
DpnII GATC 3 cut(s) 223, 228, 1045
DriI GACNNNNNGTC 1 cut(s) 1229
Eam1104I CTCTTC 4 cut(s) 40, 372, 1003, 1488
Eam1105I GACNNNNNGTC 1 cut(s) 1229
EarI CTCTTC 4 cut(s) 40, 372, 1003, 1488
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 3 cut(s) 76, 252, 351
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 2 cut(s) 967, 1252
Eco47I GGWCC 2 cut(s) 389, 434
Eco47III AGCGCT 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 241, 391
EcoRV GATATC 2 cut(s) 967, 1252
EcoT14I CCWWGG 3 cut(s) 76, 252, 351
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 3 cut(s) 76, 252, 351
FaqI GGGAC 2 cut(s) 593, 1406
Fnu4HI GCNGC 3 cut(s) 498, 594, 1054
FokI GGATG 2 cut(s) 183, 408
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 3 cut(s) 498, 594, 1054
FspBI CTAG 1 cut(s) 1440
GlaI GCGC 2 cut(s) 99, 139
GluI GCNGC 3 cut(s) 498, 594, 1054
GsuI CTGGAG 1 cut(s) 1189
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 350, 1087
HgaI GACGC 1 cut(s) 935
HhaI GCGC 2 cut(s) 100, 140
Hin6I GCGC 2 cut(s) 98, 138
HinP1I GCGC 2 cut(s) 98, 138
HindIII AAGCTT 2 cut(s) 235, 1071
HinfI GANTC 6 cut(s) 169, 356, 518, 1081, 1230, 1303
HphI GGTGA 3 cut(s) 268, 683, 882
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 5 cut(s) 228, 434, 562, 850, 872
Hpy188III TCNNGA 6 cut(s) 638, 1097, 1234, 1292, 1315, 1487
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 7 cut(s) 200, 208, 727, 940, 1208, 1254, 1370
HpyCH4III ACNGT 5 cut(s) 289, 881, 934, 1012, 1399
HpyCH4V TGCA 8 cut(s) 311, 500, 596, 683, 707, 782, 860, 1053
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
HpyF3I CTNAG 3 cut(s) 105, 613, 1340
HspAI GCGC 2 cut(s) 98, 138
Kzo9I GATC 3 cut(s) 223, 228, 1045
LmnI GCTCC 2 cut(s) 81, 416
Lsp1109I GCAGC 3 cut(s) 484, 580, 1065
LweI GCATC 2 cut(s) 153, 1423
MaeI CTAG 1 cut(s) 1440
MaeIII GTNAC 4 cut(s) 283, 1117, 1270, 1358
MalI GATC 3 cut(s) 225, 230, 1047
MboI GATC 3 cut(s) 223, 228, 1045
MboII GAAGA 8 cut(s) 27, 164, 359, 362, 597, 990, 1309, 1475
MhlI GDGCHC 4 cut(s) 313, 432, 690, 1447
MluCI AATT 7 cut(s) 127, 601, 619, 768, 855, 1040, 1504
MlyI GAGTC 2 cut(s) 527, 1239
MmeI TCCRAC 2 cut(s) 606, 1039
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 3 cut(s) 381, 789, 1507
MslI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
MspA1I CMGCKG 2 cut(s) 109, 593
MspR9I CCNGG 2 cut(s) 243, 393
Mva1269I GAATGC 3 cut(s) 88, 192, 784
MvaI CCWGG 2 cut(s) 243, 393
MwoI GCNNNNNNNGC 6 cut(s) 106, 308, 416, 836, 935, 1109
NcoI CCATGG 1 cut(s) 76
NdeII GATC 3 cut(s) 223, 228, 1045
NlaIV GGNNCC 3 cut(s) 1143, 1266, 1337
NmuCI GTSAC 2 cut(s) 1117, 1270
NspI RCATGY 1 cut(s) 1331
OliI CACNNNNGTG 1 cut(s) 1367
PaeI GCATGC 1 cut(s) 1331
PctI GAATGC 3 cut(s) 88, 192, 784
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 4 cut(s) 169, 356, 1081, 1303
PkrI GCNGC 3 cut(s) 499, 595, 1055
PleI GAGTC 2 cut(s) 526, 1238
PpsI GAGTC 2 cut(s) 526, 1238
PpuMI RGGWCCY 1 cut(s) 389
PshBI ATTAAT 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 241, 391
PspGI CCWGG 2 cut(s) 241, 391
PspN4I GGNNCC 3 cut(s) 1143, 1266, 1337
PspPI GGNCC 2 cut(s) 389, 434
PspPPI RGGWCCY 1 cut(s) 389
PstI CTGCAG 1 cut(s) 598
PvuII CAGCTG 2 cut(s) 109, 593
RsaI GTAC 3 cut(s) 113, 696, 1061
RsaNI GTAC 3 cut(s) 112, 695, 1060
RseI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 381, 789, 1507
SatI GCNGC 3 cut(s) 498, 594, 1054
Sau3AI GATC 3 cut(s) 223, 228, 1045
Sau96I GGNCC 2 cut(s) 389, 434
ScaI AGTACT 1 cut(s) 1061
SchI GAGTC 2 cut(s) 527, 1239
ScrFI CCNGG 2 cut(s) 243, 393
SduI GDGCHC 4 cut(s) 313, 432, 690, 1447
SfaNI GCATC 2 cut(s) 153, 1423
SfcI CTRYAG 1 cut(s) 594
SinI GGWCC 2 cut(s) 389, 434
SmiMI CAYNNNNRTG 3 cut(s) 453, 1113, 1367
SmlI CTYRAG 4 cut(s) 425, 515, 758, 1292
SmoI CTYRAG 4 cut(s) 425, 515, 758, 1292
SphI GCATGC 1 cut(s) 1331
Sse9I AATT 7 cut(s) 127, 601, 619, 768, 855, 1040, 1504
SsiI CCGC 2 cut(s) 1067, 1344
SspI AATATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 1440
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 241, 391
StyI CCWWGG 3 cut(s) 76, 252, 351
TaaI ACNGT 5 cut(s) 289, 881, 934, 1012, 1399
TaqI TCGA 4 cut(s) 147, 162, 1228, 1254
TaqII GACCGA 1 cut(s) 1334
TasI AATT 7 cut(s) 127, 601, 619, 768, 855, 1040, 1504
TatI WGTACW 2 cut(s) 111, 1059
TfiI GAWTC 4 cut(s) 169, 356, 1081, 1303
Tru1I TTAA 3 cut(s) 381, 789, 1507
Tru9I TTAA 3 cut(s) 381, 789, 1507
TscAI CASTG 4 cut(s) 789, 1015, 1172, 1404
TseFI GTSAC 2 cut(s) 1117, 1270
TseI GCWGC 3 cut(s) 497, 593, 1053
Tsp45I GTSAC 2 cut(s) 1117, 1270
TspGWI ACGGA 2 cut(s) 431, 1038
TspRI CASTG 4 cut(s) 789, 1015, 1172, 1404
VneI GTGCAC 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 389, 434
VspI ATTAAT 1 cut(s) 789
XapI RAATTY 2 cut(s) 127, 1040
XceI RCATGY 1 cut(s) 1331
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 1 cut(s) 1440
ZrmI AGTACT 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.