RchiOBHm_Chr1g0318981

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015798
Physical Location & Seq
Forward (+)
6629742 .. 6636414
6673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3183 bp
ATGAGGGAGAGGATGGAGGACTACTTGAGAAAAAACTTCGACGTCGAACCAAAACGACCTTCCGAAGAGGCCTTGAGGCGCTGGAGACAGGCCGTCTCCGTTGTCAAGAACCCTCGCCGCCGCTTCCGCATGGTCGCCGATCTCGCCAAGCGCGCCGAGGACGATAAAAAGCGCAAGAAACTCCAGAGAAGGAACAGTGTCAAAAATTTTGTACTACCAGATGTCGTTTCTGTCACACAGTGTGAAAAGATACGAGTAGCTCTCTATGTGCAGAAAGCAGCATTGCAATTCATCGATGCTGGTAATAAAGAAGCTGGATACAGTCTTTCCAAAGAAGTTAGTGATGCTGGTTTTGGCATTTCACCTGATGAGCTTGCATCCTTTGCGCGAACCCATGATACCAAGGGTGTGGAAGCACGAGGAGGAGTAGCAGGACTAGCAGGAGATATATCAGTATCCTTGAAAGAAGGCGTTGTTTCAAGCAAGATACCTGTTAGGCAAAACATATATGGCCTCAACCGATTTGTGGAGAAGCCTTCTAAAGGTTTTTGGATGTTTGTCTGGGAAGCCATACAAGATTTAACTCTAATCATCCTTATGGTTTCTGCTGTGGTCTCTATTGGCGTTGGCATTGCAACTGAAGGGTGGCCAAAGGGTATGTATGATGGGTTGGGTATAATATTGAGCATCTTATTGGTTGTGATGGTCACTGCAATCAGTGACTACAGGCAATCTTTGCAATTTAAAGAACTGGATAAGGAGAAAAAAAATATAAAGATTCAGGTAACTAGAGATGGACGCAGACAGAAGGTTTCTATCTACGATTTGGTGGTTGGAGATATTGTTCATCTTTCCATTGGAGACCAAGTTCCAGCTGATGGCCTTCTCATATCAGGCCACAGCTTACAAATTGATGAATCAAGCTTGTCAGGTGAGAGCGAGCCAGTGGACATAAATCAAAAGAGACCTTTTATTCTTGGAGGAACCAAAGTGCAAGATGGCTCTGGTAAAATGCTAGTTACATCTGTTGGTATGAGGACTGAATGGGGACGACTAATGGTTACTCTGAGTGAAGGAGGAGACAATGAGACACCACTGCAGGTGAAACTCAATGGTGTTGCTACCATTATTGGAAAAATTGGTTTGGCTTTTGCAGCTGTCACATTTGTAGTTCTGACTACGAGGTACTTAGTTAACAAGGGAACGCACCATAACATGACTAACTGGTCTATGAAAGATGCATCAGCACTTTTGAGTTACTTTTCTACTGCAGTTATCATAATAGTTGTTGCTGTTCCAGAGGGGCTTCCTCTGGCAGTGACACTGAGTCTTGCCTTTGCAATGAAAAAGCTAATGGGTGACAAGGCACTTGTGAGGCATCTTTCAGCATGTGAGACGATGGGCTCTGCTAGTTGTATTTGCACAGATAAGACGGGAACCTTGACAACAAATCATATGGTGGTGAATAAGATATGGATATGTGACGAAGCCAAGACTATAACGAGTGGTCAGAACAAAGATTTATTGAAGTCAGTTTCTGAAAAAGTTTATAACATTCTTTTGCAGTCTATCTTTCTGAATACAACTTCAGAGGTAGTAAAAGGGGAAGATGGGAAAAATACTGTTATAGGTACTCCAACAGAGATGGCTATAGTAGAATTTGGCATGCTTCTGGGAGGTGATTTTAAATCTTTTAGTGAGGAGTATAAGATAGTTATGGTCGAACCATTCAATTCAATCAGAAAAAAGATGTCGGTTCAAGTGGCTCTTCCTGGTAGTGATATGTGTCGAGCATTCTGCAAAGGAGCATCAGAAATTATTTTAGGAATGTGTGACAAGGTTGTCAATACTGATGGTGAAGCTATGCCTTTGTCTGAGGAGCAGAGAAAGAAAATATCTGATGTCATAAATGGTTTTGCTTGTGAAGCTTTAAGAACTCTATGCATAGCTTTCAAGGATATAGAGGACCCTTCTAGTGCAAAAAGTATTCCTGAAGATAAATATACATTAATAGCTGTTGTGGGAATTAAGGATCCTGTGCGCCCTGGCGTGAGGGAAGCCGTGAAGACTTGTTTAGATGCTGGGATTACTGTACGGATGGTCACTGGTGATAATATCAATACAGCTAAAGCCATAGCTAAAGAGTGTGGCATTTTGACAGAAGATGGTTTAGCTATAGAAGGGCCAGATTTCCAAAAAATGAGCCAACAGGAGATGGCAGAAATAATCCCAAAACTGCAGGTAATGGCACGATCCTTGCCTTTGGACAAGCACACTTTAGTGAAGCAGTTGAGGAATGTGCATAAAGAAGTAGTGGCAGTAACTGGTGATGGAACTAATGATGCTCCTGCTTTGCACGAGGCAGACATTGGACTCGCTATGGGTATAGCCGGAACAGAGGTTGCAAAAGAAAATGCTGATGTTATCATTATGGATGACAACTTTACAAGTATAGTGAATGTTGCAAGATGGGGTCGTTCAGTTTATATCAACATTCAAAAGTTTGTTCAGTTCCAGTTAACGGTTAATATTGTCGCTCTGATGCTCAATTTCGTATCAGCATGCGTCTCAGGATCAGCTCCTCTCACTGCTGTCCAGATGCTTTGGGTAAACTTGATTATGGATACTCTTGGCGCTTTGGCACTGGCTACAGAGCCACCGCATGATGGCCTTATGAAAAGACCACCAGTTGGAAGGAATATTAAATTCATCACTGGGATCATGTGGAGGAATATTATCGGTCAGAGTATCTATCAAATTGCTGTCCTTCTGGTTCTTAATTTTTGTGGGAAACGTCTTTTAAAACTCACCGGTCCAAATGCAAGTTCCATTCTCAAGACCTTCATATTCAACTGTTTTGTATTTTGCCAGGTATTCAACGAGATTAACAGCCGTGATATGGAGAAAATCAATGTGTTCAGAGGGATGTTTAATAGTTATACTTTCATGATAGTGATGATCTGCACAGTAGTTTTCCAGATCATAATAGTTGAATTTTTGGGCACTTTCGCTCAAACTGTGCCACTGAGCTGGAAGTTGTGGTTATCAAGCGTCTTGATTGGAGCCGTAAGTTTACCAGTTGCTGTTGTCTTGAAGTGCATTCCTGTATCATTAAGGAAACAAGCTGCTAAGGGTCACAGTGATATCCACGAACCTCTTCTCCGTGGCCCTGAGCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1060

Amino Acids

116.24

Weight (kDa)

8.74

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 9 - 53 7.4e-22 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 140 - 206 2.3e-10 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 259 - 353 1.1e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 519 - 682 3.2e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 665 - 789 6.5e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 860 - 1033 1.1e-40 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1551
AarI CACCTGC 1 cut(s) 1090
AasI GACNNNNNNGTC 2 cut(s) 1225, 1841
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 2 cut(s) 1090, 2230
AccB7I CCANNNNNTGG 2 cut(s) 878, 2690
AccII CGCG 2 cut(s) 153, 388
AciI CCGC 4 cut(s) 118, 121, 127, 2660
AclWI GGATC 5 cut(s) 2027, 2040, 2247, 2581, 2726
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 4 cut(s) 205, 1658, 2705, 2993
AcuI CTGAAG 3 cut(s) 660, 1572, 2013
AcyI GRCGYC 1 cut(s) 42
AdeI CACNNNGTG 2 cut(s) 240, 242
AfaI GTAC 4 cut(s) 213, 1187, 1633, 2094
AfiI CCNNNNNNNGG 8 cut(s) 526, 542, 878, 2521, 2666, 2690, 2899, 3175
AgeI ACCGGT 1 cut(s) 2810
AhdI GACNNNNNGTC 2 cut(s) 92, 1326
AjnI CCWGG 3 cut(s) 1771, 2044, 2868
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 2809, 2841
AleI CACNNNNGTG 1 cut(s) 2279
AloI GAACNNNNNNTCC 6 cut(s) 828, 852, 860, 884, 3144, 3176
Alw26I GTCTC 9 cut(s) 79, 100, 619, 855, 958, 1074, 1082, 1388, 2572
AlwI GGATC 5 cut(s) 2027, 2040, 2247, 2581, 2726
AlwNI CAGNNNCTG 3 cut(s) 1538, 2324, 3083
AoxI GGCC 9 cut(s) 69, 90, 511, 647, 880, 895, 2183, 2668, 3166
ApeKI GCWGC 3 cut(s) 278, 1154, 3125
ApoI RAATTY 4 cut(s) 205, 1658, 2705, 2993
AseI ATTAAT 1 cut(s) 2009
AsiGI ACCGGT 1 cut(s) 2810
Asp700I GAANNNNTTC 1 cut(s) 3156
AspLEI GCGC 7 cut(s) 81, 153, 155, 174, 388, 2043, 2636
AspS9I GGNCC 4 cut(s) 1966, 2183, 2813, 3167
AvaII GGWCC 2 cut(s) 1966, 2813
BaeGI GKGCMC 1 cut(s) 3005
BalI TGGCCA 1 cut(s) 649
BamHI GGATCC 1 cut(s) 2032
BanII GRGCYC 1 cut(s) 1406
BauI CACGAG 2 cut(s) 417, 2357
BbsI GAAGAC 1 cut(s) 2072
BbvI GCAGC 3 cut(s) 290, 1166, 3112
BceAI ACGGC 4 cut(s) 77, 2045, 2877, 3050
BcgI CGANNNNNNTGC 2 cut(s) 1779, 1813
BciT130I CCWGG 3 cut(s) 1773, 2046, 2870
BciVI GTATCC 3 cut(s) 311, 466, 2617
BcoDI GTCTC 9 cut(s) 79, 100, 619, 855, 958, 1074, 1082, 1388, 2572
BfaI CTAG 5 cut(s) 437, 789, 1016, 1410, 1974
BfmI CTRYAG 7 cut(s) 724, 1097, 1269, 1650, 2175, 2237, 2649
BfoI RGCGCY 2 cut(s) 82, 2637
BfuAI ACCTGC 2 cut(s) 1090, 2230
BfuI GTATCC 3 cut(s) 311, 466, 2617
BisI GCNGC 5 cut(s) 118, 121, 279, 1155, 3126
BlsI GCNGC 5 cut(s) 119, 122, 280, 1156, 3127
Bme1390I CCNGG 3 cut(s) 1773, 2046, 2870
Bme18I GGWCC 2 cut(s) 1966, 2813
BmeRI GACNNNNNGTC 2 cut(s) 92, 1326
BmgT120I GGNCC 4 cut(s) 1966, 2183, 2813, 3167
BmiI GGNNCC 5 cut(s) 985, 1438, 1968, 2034, 3064
BmrFI CCNGG 3 cut(s) 1773, 2046, 2870
BmrI ACTGGG 1 cut(s) 2724
BmuI ACTGGG 1 cut(s) 2724
BpiI GAAGAC 1 cut(s) 2072
BplI GAGNNNNNCTC 2 cut(s) 246, 278
BpmI CTGGAG 2 cut(s) 103, 167
Bpu10I CCTNAGC 2 cut(s) 3129, 3171
BpuEI CTTGAG 3 cut(s) 46, 94, 2819
Bsa29I ATCGAT 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 3 cut(s) 619, 855, 958
BsaJI CCNNGG 4 cut(s) 156, 402, 2044, 3163
BsaWI WCCGGW 1 cut(s) 2810
BsaXI ACNNNNNCTCC 6 cut(s) 828, 852, 858, 882, 3144, 3174
Bsc4I CCNNNNNNNGG 8 cut(s) 526, 542, 878, 2521, 2666, 2690, 2899, 3175
Bse118I RCCGGY 1 cut(s) 2810
Bse3DI GCAATG 3 cut(s) 281, 630, 1347
BseBI CCWGG 3 cut(s) 1773, 2046, 2870
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 4 cut(s) 156, 402, 2044, 3163
BseGI GGATG 7 cut(s) 18, 377, 558, 591, 2103, 2440, 2931
BseLI CCNNNNNNNGG 8 cut(s) 526, 542, 878, 2521, 2666, 2690, 2899, 3175
BseMI GCAATG 3 cut(s) 281, 630, 1347
BseMII CTCAG 6 cut(s) 1058, 1316, 1866, 2583, 3017, 3162
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 6 cut(s) 435, 438, 1092, 1715, 1892, 2571
BseSI GKGCMC 1 cut(s) 3005
BseXI GCAGC 3 cut(s) 290, 1166, 3112
BseYI CCCAGC 1 cut(s) 2081
BsgI GTGCAG 2 cut(s) 290, 2947
Bsh1236I CGCG 2 cut(s) 153, 388
BshFI GGCC 9 cut(s) 71, 92, 513, 649, 882, 897, 2185, 2670, 3168
BshTI ACCGGT 1 cut(s) 2810
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 2 cut(s) 2391, 2811
BslFI GGGAC 1 cut(s) 1062
BslI CCNNNNNNNGG 8 cut(s) 526, 542, 878, 2521, 2666, 2690, 2899, 3175
BsmAI GTCTC 9 cut(s) 79, 100, 619, 855, 958, 1074, 1082, 1388, 2572
BsmBI CGTCTC 3 cut(s) 100, 1388, 2572
BsmFI GGGAC 1 cut(s) 1062
BsmI GAATGC 2 cut(s) 1793, 3099
BsnI GGCC 9 cut(s) 71, 92, 513, 649, 882, 897, 2185, 2670, 3168
Bso31I GGTCTC 3 cut(s) 619, 855, 958
Bsp1286I GDGCHC 2 cut(s) 1406, 3005
Bsp143I GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
BspACI CCGC 4 cut(s) 118, 121, 127, 2660
BspANI GGCC 9 cut(s) 71, 92, 513, 649, 882, 897, 2185, 2670, 3168
BspCNI CTCAG 6 cut(s) 1059, 1317, 1867, 2582, 3018, 3163
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 2 cut(s) 153, 388
BspHI TCATGA 1 cut(s) 2946
BspLI GGNNCC 5 cut(s) 985, 1438, 1968, 2034, 3064
BspMAI CTGCAG 3 cut(s) 1101, 1273, 2241
BspMI ACCTGC 2 cut(s) 1090, 2230
BspPI GGATC 5 cut(s) 2027, 2040, 2247, 2581, 2726
BspQI GCTCTTC 1 cut(s) 1773
BspTNI GGTCTC 3 cut(s) 619, 855, 958
BsrDI GCAATG 3 cut(s) 281, 630, 1347
BsrFI RCCGGY 1 cut(s) 2810
BssAI RCCGGY 1 cut(s) 2810
BssECI CCNNGG 4 cut(s) 156, 402, 2044, 3163
BssHII GCGCGC 1 cut(s) 151
BssMI GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 417, 2357
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 2 cut(s) 417, 2357
Bst2UI CCWGG 3 cut(s) 1773, 2046, 2870
Bst6I CTCTTC 3 cut(s) 60, 1773, 3162
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 383, 736
BstC8I GCNNGC 6 cut(s) 153, 375, 941, 1667, 2563, 3177
BstDEI CTNAG 8 cut(s) 1067, 1189, 1325, 1875, 2569, 3026, 3129, 3171
BstDSI CCRYGG 1 cut(s) 3163
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 7 cut(s) 18, 377, 558, 591, 2103, 2440, 2931
BstFNI CGCG 2 cut(s) 153, 388
BstH2I RGCGCY 2 cut(s) 82, 2637
BstHHI GCGC 7 cut(s) 81, 153, 155, 174, 388, 2043, 2636
BstKTI GATC 7 cut(s) 142, 2035, 2255, 2576, 2721, 2961, 2982
BstMAI GTCTC 9 cut(s) 79, 100, 619, 855, 958, 1074, 1082, 1388, 2572
BstMBI GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
BstMWI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 383, 437, 736, 1154, 1925
BstNI CCWGG 3 cut(s) 1773, 2046, 2870
BstNSI RCATGY 3 cut(s) 1392, 1669, 2565
BstSCI CCNGG 3 cut(s) 1771, 2044, 2868
BstSFI CTRYAG 7 cut(s) 724, 1097, 1269, 1650, 2175, 2237, 2649
BstSLI GKGCMC 1 cut(s) 3005
BstUI CGCG 2 cut(s) 153, 388
BstV1I GCAGC 3 cut(s) 290, 1166, 3112
BstV2I GAAGAC 1 cut(s) 2072
BstX2I RGATCY 1 cut(s) 2032
BstXI CCANNNNNNTGG 2 cut(s) 409, 3030
BstYI RGATCY 1 cut(s) 2032
Bsu15I ATCGAT 1 cut(s) 294
BsuI GTATCC 3 cut(s) 311, 466, 2617
BsuRI GGCC 9 cut(s) 71, 92, 513, 649, 882, 897, 2185, 2670, 3168
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 3163
BtsCI GGATG 7 cut(s) 18, 377, 558, 591, 2103, 2440, 2931
BtsI GCAGTG 4 cut(s) 708, 1094, 1323, 2586
BveI ACCTGC 2 cut(s) 1090, 2230
Cac8I GCNNGC 6 cut(s) 153, 375, 941, 1667, 2563, 3177
CaiI CAGNNNCTG 3 cut(s) 1538, 2324, 3083
CciI TCATGA 1 cut(s) 2946
CfoI GCGC 7 cut(s) 81, 153, 155, 174, 388, 2043, 2636
Cfr10I RCCGGY 1 cut(s) 2810
Cfr13I GGNCC 4 cut(s) 1966, 2183, 2813, 3167
ClaI ATCGAT 1 cut(s) 294
CseI GACGC 3 cut(s) 807, 2554, 3040
Csp6I GTAC 4 cut(s) 212, 1186, 1632, 2093
CspAI ACCGGT 1 cut(s) 2810
CviAII CATG 9 cut(s) 130, 395, 1216, 1389, 1666, 2562, 2663, 2722, 2947
CviQI GTAC 4 cut(s) 212, 1186, 1632, 2093
DdeI CTNAG 8 cut(s) 1067, 1189, 1325, 1875, 2569, 3026, 3129, 3171
DpnI GATC 7 cut(s) 141, 2034, 2254, 2575, 2720, 2960, 2981
DpnII GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
DraI TTTAAA 3 cut(s) 745, 1687, 2802
DraIII CACNNNGTG 2 cut(s) 240, 242
DrdI GACNNNNNNGTC 2 cut(s) 1225, 1841
DriI GACNNNNNGTC 2 cut(s) 92, 1326
DseDI GACNNNNNNGTC 2 cut(s) 1225, 1841
EaeI YGGCCR 1 cut(s) 647
Eam1104I CTCTTC 3 cut(s) 60, 1773, 3162
Eam1105I GACNNNNNGTC 2 cut(s) 92, 1326
EarI CTCTTC 3 cut(s) 60, 1773, 3162
Eco130I CCWWGG 1 cut(s) 402
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 1406
Eco31I GGTCTC 3 cut(s) 619, 855, 958
Eco32I GATATC 1 cut(s) 3145
Eco47I GGWCC 2 cut(s) 1966, 2813
Eco57I CTGAAG 3 cut(s) 660, 1572, 2013
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 1 cut(s) 1966
EcoRII CCWGG 3 cut(s) 1771, 2044, 2868
EcoRV GATATC 1 cut(s) 3145
EcoT14I CCWWGG 1 cut(s) 402
EcoT22I ATGCAT 2 cut(s) 1243, 1946
EcoT38I GRGCYC 1 cut(s) 1406
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 3 cut(s) 100, 1388, 2572
FaeI CATG 9 cut(s) 133, 398, 1219, 1392, 1669, 2565, 2666, 2725, 2950
FalI AAGNNNNNCTT 2 cut(s) 1752, 1784
FaqI GGGAC 1 cut(s) 1062
FatI CATG 9 cut(s) 129, 394, 1215, 1388, 1665, 2561, 2662, 2721, 2946
FauNDI CATATG 1 cut(s) 1455
Fnu4HI GCNGC 5 cut(s) 118, 121, 279, 1155, 3126
FokI GGATG 7 cut(s) 25, 364, 565, 578, 2110, 2447, 2938
FriOI GRGCYC 1 cut(s) 1406
Fsp4HI GCNGC 5 cut(s) 118, 121, 279, 1155, 3126
FspBI CTAG 5 cut(s) 437, 789, 1016, 1410, 1974
GlaI GCGC 7 cut(s) 80, 152, 154, 173, 387, 2042, 2635
GluI GCNGC 5 cut(s) 118, 121, 279, 1155, 3126
GsaI CCCAGC 1 cut(s) 2085
GsuI CTGGAG 2 cut(s) 103, 167
HaeII RGCGCY 2 cut(s) 82, 2637
HaeIII GGCC 9 cut(s) 71, 92, 513, 649, 882, 897, 2185, 2670, 3168
HapII CCGG 2 cut(s) 2391, 2811
HgaI GACGC 3 cut(s) 807, 2554, 3040
HhaI GCGC 7 cut(s) 81, 153, 155, 174, 388, 2043, 2636
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 9 cut(s) 133, 398, 1219, 1392, 1669, 2565, 2666, 2725, 2950
Hin6I GCGC 7 cut(s) 79, 151, 153, 172, 386, 2041, 2634
HinP1I GCGC 7 cut(s) 79, 151, 153, 172, 386, 2041, 2634
HincII GTYRAC 2 cut(s) 1195, 2520
HindII GTYRAC 2 cut(s) 1195, 2520
HindIII AAGCTT 2 cut(s) 922, 1926
HinfI GANTC 4 cut(s) 778, 917, 1327, 2373
HpaI GTTAAC 2 cut(s) 1195, 2520
HpaII CCGG 2 cut(s) 2391, 2811
Hpy166II GTNNAC 5 cut(s) 949, 1195, 2520, 2611, 3074
Hpy8I GTNNAC 5 cut(s) 949, 1195, 2520, 2611, 3074
Hpy99I CGWCG 2 cut(s) 44, 47
HpyCH4IV ACGT 2 cut(s) 42, 2794
HpyF10VI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 383, 437, 736, 1154, 1925
HpyF3I CTNAG 8 cut(s) 1067, 1189, 1325, 1875, 2569, 3026, 3129, 3171
HpySE526I ACGT 2 cut(s) 42, 2794
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 9 cut(s) 133, 398, 1219, 1392, 1669, 2565, 2666, 2725, 2950
HspAI GCGC 7 cut(s) 79, 151, 153, 172, 386, 2041, 2634
KspAI GTTAAC 2 cut(s) 1195, 2520
Kzo9I GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
LguI GCTCTTC 1 cut(s) 1773
LmnI GCTCC 5 cut(s) 1805, 1879, 2350, 2584, 3062
Lsp1109I GCAGC 3 cut(s) 290, 1166, 3112
MaeI CTAG 5 cut(s) 437, 789, 1016, 1410, 1974
MaeII ACGT 2 cut(s) 42, 2794
MalI GATC 7 cut(s) 141, 2034, 2254, 2575, 2720, 2960, 2981
MboI GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
MboII GAAGA 7 cut(s) 77, 1619, 1760, 2006, 2077, 2174, 3149
MflI RGATCY 1 cut(s) 2032
MhlI GDGCHC 2 cut(s) 1406, 3005
MlsI TGGCCA 1 cut(s) 649
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 2 cut(s) 1336, 2367
MmeI TCCRAC 3 cut(s) 814, 1661, 2671
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 2 cut(s) 1243, 1946
MroXI GAANNNNTTC 1 cut(s) 3156
MscI TGGCCA 1 cut(s) 649
MslI CAYNNNNRTG 3 cut(s) 596, 2279, 2951
Msp20I TGGCCA 1 cut(s) 649
MspA1I CMGCKG 2 cut(s) 875, 1157
MspI CCGG 2 cut(s) 2391, 2811
MspR9I CCNGG 3 cut(s) 1773, 2046, 2870
Mva1269I GAATGC 2 cut(s) 1793, 3099
MvaI CCWGG 3 cut(s) 1773, 2046, 2870
MvnI CGCG 2 cut(s) 153, 388
MwoI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 383, 437, 736, 1154, 1925
NdeI CATATG 1 cut(s) 1455
NdeII GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
NlaIII CATG 9 cut(s) 133, 398, 1219, 1392, 1669, 2565, 2666, 2725, 2950
NlaIV GGNNCC 5 cut(s) 985, 1438, 1968, 2034, 3064
NmeAIII GCCGAG 1 cut(s) 181
NsiI ATGCAT 2 cut(s) 1243, 1946
NspI RCATGY 3 cut(s) 1392, 1669, 2565
OliI CACNNNNGTG 1 cut(s) 2279
PaeI GCATGC 2 cut(s) 1669, 2565
PagI TCATGA 1 cut(s) 2946
PaqCI CACCTGC 1 cut(s) 1090
PauI GCGCGC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 1773
PctI GAATGC 2 cut(s) 1793, 3099
PdmI GAANNNNTTC 1 cut(s) 3156
PfeI GAWTC 2 cut(s) 778, 917
PflMI CCANNNNNTGG 2 cut(s) 878, 2690
PinAI ACCGGT 1 cut(s) 2810
PkrI GCNGC 5 cut(s) 119, 122, 280, 1156, 3127
PleI GAGTC 2 cut(s) 1335, 2367
PpsI GAGTC 2 cut(s) 1335, 2367
PpuMI RGGWCCY 1 cut(s) 1966
PshBI ATTAAT 1 cut(s) 2009
PsiI TTATAA 1 cut(s) 1551
Psp5II RGGWCCY 1 cut(s) 1966
Psp6I CCWGG 3 cut(s) 1771, 2044, 2868
PspFI CCCAGC 1 cut(s) 2081
PspGI CCWGG 3 cut(s) 1771, 2044, 2868
PspN4I GGNNCC 5 cut(s) 985, 1438, 1968, 2034, 3064
PspPI GGNCC 4 cut(s) 1966, 2183, 2813, 3167
PspPPI RGGWCCY 1 cut(s) 1966
PsrI GAACNNNNNNTAC 2 cut(s) 382, 414
PstI CTGCAG 3 cut(s) 1101, 1273, 2241
PstNI CAGNNNCTG 3 cut(s) 1538, 2324, 3083
PsuI RGATCY 1 cut(s) 2032
PteI GCGCGC 1 cut(s) 151
PvuII CAGCTG 2 cut(s) 875, 1157
RsaI GTAC 4 cut(s) 213, 1187, 1633, 2094
RsaNI GTAC 4 cut(s) 212, 1186, 1632, 2093
RseI CAYNNNNRTG 3 cut(s) 596, 2279, 2951
SapI GCTCTTC 1 cut(s) 1773
SatI GCNGC 5 cut(s) 118, 121, 279, 1155, 3126
Sau3AI GATC 7 cut(s) 139, 2032, 2252, 2573, 2718, 2958, 2979
Sau96I GGNCC 4 cut(s) 1966, 2183, 2813, 3167
SchI GAGTC 2 cut(s) 1336, 2367
ScrFI CCNGG 3 cut(s) 1773, 2046, 2870
SduI GDGCHC 2 cut(s) 1406, 3005
SfcI CTRYAG 7 cut(s) 724, 1097, 1269, 1650, 2175, 2237, 2649
SinI GGWCC 2 cut(s) 1966, 2813
SmiMI CAYNNNNRTG 3 cut(s) 596, 2279, 2951
SmlI CTYRAG 3 cut(s) 25, 73, 2834
SmoI CTYRAG 3 cut(s) 25, 73, 2834
SphI GCATGC 2 cut(s) 1669, 2565
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 4 cut(s) 118, 121, 127, 2660
SspI AATATT 4 cut(s) 681, 2530, 2701, 2734
SspMI CTAG 5 cut(s) 437, 789, 1016, 1410, 1974
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 1771, 2044, 2868
StyI CCWWGG 1 cut(s) 402
TaiI ACGT 2 cut(s) 45, 2797
TaqI TCGA 5 cut(s) 39, 45, 294, 1722, 1789
TaqII GACCGA 1 cut(s) 2729
TatI WGTACW 1 cut(s) 211
TauI GCSGC 2 cut(s) 120, 123
TfiI GAWTC 2 cut(s) 778, 917
TseI GCWGC 3 cut(s) 278, 1154, 3125
TspDTI ATGAA 9 cut(s) 280, 836, 930, 1247, 1358, 2690, 2698, 2833, 2935
TspGWI ACGGA 3 cut(s) 88, 2110, 3152
Van91I CCANNNNNTGG 2 cut(s) 878, 2690
VpaK11BI GGWCC 2 cut(s) 1966, 2813
VspI ATTAAT 1 cut(s) 2009
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 4 cut(s) 205, 1658, 2705, 2993
XceI RCATGY 3 cut(s) 1392, 1669, 2565
XmnI GAANNNNTTC 1 cut(s) 3156
XspI CTAG 5 cut(s) 437, 789, 1016, 1410, 1974
ZraI GACGTC 1 cut(s) 43
Zsp2I ATGCAT 2 cut(s) 1243, 1946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.