RchiOBHm_Chr1g0349031

Holliday junction resolvase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016704
Physical Location & Seq
Forward (+)
42092910 .. 42095893
2984 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGACCCCAGTAAGATGTATTCATTTAAGCCATAGTACAGTTTTATATTGGCCATTGAACTCGCATCATTCTGCAACTCTGCCCCCAAATGAAAGAAAAGATTTTAACGACAAACGTTCGTCGGGTAAACACAAGGATGGGAATAAACGATTTCAAACTACAAAGCCTATCCCACCTCACTTGGGTCCTCAGAAAATGTCTCGTCACCCACTCGGAGCTGCTCTCCCGGCGCACACCGAACTCCTCATTCCGATCAAAAACGCCGCCGTTCTGAACCTCAAAACCGCCCCCACCTCTCCTCAACTCTCTCTCACCCAAACCCGTAAACCCTGCCCTGCAATAAGGGCAGTCTCCATCGATGAAATTCCCCCAAATGCCCTCCGCCGGAAGCGCGACTCCAACTGGAGAGGAGGCTTTAGCGTAGGGGTAGACTTGGGATTGTCCCGCACCGGCCTCGCCCTCAGCAAAGGCTTCTCCGTCCGTCCCCTCACCGTGTTGAATTTGAGAGGGCAGAAGCTTGAGGTTAAAATCCTTGAGATTGCAAAGCAAGAGGAGGCTGATGAGTTTATAATCGGGCTTCCGAAATCGAGTGATGGGAAAGAGACACCTCAGTCTAACAAAGTTCGTAGTGTCGCCGGCAGACTTGCTGCTACTGCTGCTGAGAGGGGTTGGAGAGTATACCTGCAAGATGAAAATGGGACATCCATCGAAGCCATGGATCGAATGATTAACATGGGCCTCAGCAGGTCTGATCGTCAAAGCAAAATTGATGCCTACGCTGCCATGATGGTGCTGGAGCGATATTTTTCCATGTCAGGTGAAGGAACTGAGCTTGTACTGCCCAGGAATTTGGATCTGCAAGACAGACTTCGAAGAGGTCCACCCAAAGACGTTGATTACTTCTCTGAAGACGATGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RuvX

Protein Analysis

307

Amino Acids

34.17

Weight (kDa)

9.61

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RuvX PF03652 141 - 270 8.8e-26 Holliday junction resolvase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12244
fragaria_vesca FvH4_7g11040
malus_domestica MD07G1114500.v1.1
prunus_persica Prupe.2G137100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349031
rosa_laevigata RLG00000028610
rosa_multiflora Rmu_sc0038026.1_g000002
rosa_roxburghii Rroxscaffold_4G00306220
rosa_rugosa Rorug01G0199600
rosa_samantha Rh1AG217200 Rh1BG182700 Rh1CG201600 Rh1DG212700
rosa_wichuraiana Rw1G018470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AasI GACNNNNNNGTC 1 cut(s) 610
Acc36I ACCTGC 2 cut(s) 690, 735
AccI GTMKAC 2 cut(s) 429, 678
AccII CGCG 1 cut(s) 393
AciI CCGC 4 cut(s) 264, 285, 382, 445
AclI AACGTT 1 cut(s) 115
AclWI GGATC 2 cut(s) 726, 861
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 3 cut(s) 363, 499, 847
AfaI GTAC 2 cut(s) 37, 837
AfiI CCNNNNNNNGG 2 cut(s) 182, 384
AgsI TTSAA 3 cut(s) 58, 155, 499
AjnI CCWGG 1 cut(s) 842
AluBI AGCT 3 cut(s) 218, 517, 832
AluI AGCT 3 cut(s) 218, 517, 832
Alw26I GTCTC 3 cut(s) 204, 355, 596
AlwI GGATC 2 cut(s) 726, 861
AoxI GGCC 3 cut(s) 50, 451, 736
ApeKI GCWGC 4 cut(s) 218, 647, 656, 779
ApoI RAATTY 3 cut(s) 363, 499, 847
AspLEI GCGC 2 cut(s) 232, 393
AspS9I GGNCC 3 cut(s) 185, 736, 878
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 4 cut(s) 197, 304, 481, 830
AsuII TTCGAA 1 cut(s) 871
AvaII GGWCC 2 cut(s) 185, 878
BalI TGGCCA 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 915
BbvCI CCTCAGC 2 cut(s) 461, 740
BbvI GCAGC 4 cut(s) 205, 634, 643, 766
BccI CCATC 5 cut(s) 131, 362, 587, 713, 781
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 1 cut(s) 844
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 204, 355, 596
BfuAI ACCTGC 2 cut(s) 690, 735
BisI GCNGC 5 cut(s) 219, 264, 648, 657, 780
BlsI GCNGC 5 cut(s) 220, 265, 649, 658, 781
Bme1390I CCNGG 2 cut(s) 227, 844
Bme18I GGWCC 2 cut(s) 185, 878
BmgT120I GGNCC 3 cut(s) 185, 736, 878
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 2 cut(s) 227, 844
BmsI GCATC 2 cut(s) 73, 760
BpiI GAAGAC 1 cut(s) 915
BplI GAGNNNNNCTC 2 cut(s) 207, 239
BpmI CTGGAG 2 cut(s) 424, 815
Bpu10I CCTNAGC 2 cut(s) 461, 740
Bpu14I TTCGAA 1 cut(s) 871
BpuEI CTTGAG 2 cut(s) 539, 554
BpuMI CCSGG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 714, 842
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 384
Bse118I RCCGGY 2 cut(s) 449, 635
Bse1I ACTGG 2 cut(s) 8, 407
BseBI CCWGG 1 cut(s) 844
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 714, 842
BseGI GGATG 2 cut(s) 142, 701
BseLI CCNNNNNNNGG 2 cut(s) 182, 384
BseMII CTCAG 6 cut(s) 203, 475, 623, 651, 754, 819
BseNI ACTGG 2 cut(s) 8, 407
BseRI GAGGAG 4 cut(s) 233, 288, 423, 566
BseXI GCAGC 4 cut(s) 205, 634, 643, 766
Bsh1236I CGCG 1 cut(s) 393
BshFI GGCC 3 cut(s) 52, 453, 738
BshVI ATCGAT 1 cut(s) 357
BsiSI CCGG 4 cut(s) 227, 385, 450, 636
BslFI GGGAC 3 cut(s) 427, 468, 712
BslI CCNNNNNNNGG 2 cut(s) 182, 384
BsmAI GTCTC 3 cut(s) 204, 355, 596
BsmFI GGGAC 3 cut(s) 427, 468, 712
BsnI GGCC 3 cut(s) 52, 453, 738
Bsp119I TTCGAA 1 cut(s) 871
Bsp143I GATC 4 cut(s) 252, 718, 751, 853
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 4 cut(s) 264, 285, 382, 445
BspANI GGCC 3 cut(s) 52, 453, 738
BspCNI CTCAG 6 cut(s) 202, 474, 622, 652, 753, 820
BspDI ATCGAT 1 cut(s) 357
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 1 cut(s) 186
BspMI ACCTGC 2 cut(s) 690, 735
BspPI GGATC 2 cut(s) 726, 861
BspT104I TTCGAA 1 cut(s) 871
BsrFI RCCGGY 2 cut(s) 449, 635
BsrI ACTGG 2 cut(s) 8, 407
BssAI RCCGGY 2 cut(s) 449, 635
BssECI CCNNGG 2 cut(s) 714, 842
BssMI GATC 4 cut(s) 252, 718, 751, 853
BssNAI GTATAC 1 cut(s) 679
BssT1I CCWWGG 1 cut(s) 714
Bst1107I GTATAC 1 cut(s) 679
Bst2UI CCWGG 1 cut(s) 844
Bst4CI ACNGT 2 cut(s) 40, 493
Bst6I CTCTTC 1 cut(s) 868
BstBI TTCGAA 1 cut(s) 871
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 6 cut(s) 189, 461, 609, 660, 740, 828
BstDSI CCRYGG 1 cut(s) 714
BstF5I GGATG 2 cut(s) 142, 701
BstFNI CGCG 1 cut(s) 393
BstHHI GCGC 2 cut(s) 232, 393
BstKTI GATC 4 cut(s) 255, 721, 754, 856
BstMAI GTCTC 3 cut(s) 204, 355, 596
BstMBI GATC 4 cut(s) 252, 718, 751, 853
BstMWI GCNNNNNNNGC 7 cut(s) 227, 344, 390, 653, 656, 779, 838
BstNI CCWGG 1 cut(s) 844
BstSCI CCNGG 2 cut(s) 225, 842
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 4 cut(s) 205, 634, 643, 766
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 1 cut(s) 853
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 1 cut(s) 853
BstZ17I GTATAC 1 cut(s) 679
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 3 cut(s) 52, 453, 738
BsuTUI ATCGAT 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 714
BtsCI GGATG 2 cut(s) 142, 701
BveI ACCTGC 2 cut(s) 690, 735
Cac8I GCNNGC 1 cut(s) 637
CfoI GCGC 2 cut(s) 232, 393
Cfr10I RCCGGY 2 cut(s) 449, 635
Cfr13I GGNCC 3 cut(s) 185, 736, 878
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 2 cut(s) 36, 836
CspCI CAANNNNNGTGG 2 cut(s) 162, 197
CviAII CATG 4 cut(s) 715, 733, 784, 811
CviQI GTAC 2 cut(s) 36, 836
DdeI CTNAG 6 cut(s) 189, 461, 609, 660, 740, 828
DpnI GATC 4 cut(s) 254, 720, 753, 855
DpnII GATC 4 cut(s) 252, 718, 751, 853
DrdI GACNNNNNNGTC 1 cut(s) 610
DseDI GACNNNNNNGTC 1 cut(s) 610
EaeI YGGCCR 1 cut(s) 50
Eam1104I CTCTTC 1 cut(s) 868
EarI CTCTTC 1 cut(s) 868
EciI GGCGGA 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 714
Eco47I GGWCC 2 cut(s) 185, 878
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 842
EcoT14I CCWWGG 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 714
FaeI CATG 4 cut(s) 718, 736, 787, 814
FaiI YATR 8 cut(s) 33, 46, 569, 679, 716, 734, 785, 812
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 3 cut(s) 427, 468, 712
FatI CATG 4 cut(s) 714, 732, 783, 810
FauI CCCGC 1 cut(s) 452
FblI GTMKAC 2 cut(s) 429, 678
Fnu4HI GCNGC 5 cut(s) 219, 264, 648, 657, 780
FokI GGATG 2 cut(s) 149, 688
Fsp4HI GCNGC 5 cut(s) 219, 264, 648, 657, 780
GlaI GCGC 2 cut(s) 231, 392
GluI GCNGC 5 cut(s) 219, 264, 648, 657, 780
GsuI CTGGAG 2 cut(s) 424, 815
HaeIII GGCC 3 cut(s) 52, 453, 738
HapII CCGG 4 cut(s) 227, 385, 450, 636
HhaI GCGC 2 cut(s) 232, 393
Hin1II CATG 4 cut(s) 718, 736, 787, 814
Hin6I GCGC 2 cut(s) 230, 391
HinP1I GCGC 2 cut(s) 230, 391
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 1 cut(s) 395
HpaII CCGG 4 cut(s) 227, 385, 450, 636
HphI GGTGA 4 cut(s) 197, 304, 481, 830
Hpy166II GTNNAC 5 cut(s) 128, 326, 430, 679, 881
Hpy188I TCNGA 7 cut(s) 192, 215, 252, 273, 582, 751, 907
Hpy8I GTNNAC 5 cut(s) 128, 326, 430, 679, 881
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 815
HpyCH4III ACNGT 2 cut(s) 40, 493
HpyCH4IV ACGT 2 cut(s) 115, 891
HpyCH4V TGCA 5 cut(s) 74, 338, 542, 685, 859
HpyF10VI GCNNNNNNNGC 7 cut(s) 227, 344, 390, 653, 656, 779, 838
HpyF3I CTNAG 6 cut(s) 189, 461, 609, 660, 740, 828
HpySE526I ACGT 2 cut(s) 115, 891
Hsp92II CATG 4 cut(s) 718, 736, 787, 814
HspAI GCGC 2 cut(s) 230, 391
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Kzo9I GATC 4 cut(s) 252, 718, 751, 853
LmnI GCTCC 2 cut(s) 215, 796
Lsp1109I GCAGC 4 cut(s) 205, 634, 643, 766
LweI GCATC 2 cut(s) 73, 760
MaeII ACGT 2 cut(s) 115, 891
MaeIII GTNAC 1 cut(s) 203
MalI GATC 4 cut(s) 254, 720, 753, 855
MboI GATC 4 cut(s) 252, 718, 751, 853
MboII GAAGA 2 cut(s) 885, 920
MflI RGATCY 1 cut(s) 853
MlsI TGGCCA 1 cut(s) 52
MluCI AATT 4 cut(s) 363, 499, 765, 847
MluNI TGGCCA 1 cut(s) 52
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 423, 650
Mox20I TGGCCA 1 cut(s) 52
MroNI GCCGGC 1 cut(s) 635
MscI TGGCCA 1 cut(s) 52
MseI TTAA 4 cut(s) 26, 105, 525, 729
MslI CAYNNNNRTG 2 cut(s) 135, 788
Msp20I TGGCCA 1 cut(s) 52
MspI CCGG 4 cut(s) 227, 385, 450, 636
MspR9I CCNGG 2 cut(s) 227, 844
MvaI CCWGG 1 cut(s) 844
MvnI CGCG 1 cut(s) 393
MwoI GCNNNNNNNGC 7 cut(s) 227, 344, 390, 653, 656, 779, 838
NaeI GCCGGC 1 cut(s) 637
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 714
NdeII GATC 4 cut(s) 252, 718, 751, 853
NgoMIV GCCGGC 1 cut(s) 635
NlaIII CATG 4 cut(s) 718, 736, 787, 814
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 203
NspV TTCGAA 1 cut(s) 871
PdiI GCCGGC 1 cut(s) 637
PkrI GCNGC 5 cut(s) 220, 265, 649, 658, 781
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 185
PsiI TTATAA 1 cut(s) 569
Psp1406I AACGTT 1 cut(s) 115
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 842
PspGI CCWGG 1 cut(s) 842
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 3 cut(s) 185, 736, 878
PspPPI RGGWCCY 1 cut(s) 185
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 2 cut(s) 37, 837
RsaNI GTAC 2 cut(s) 36, 836
RseI CAYNNNNRTG 2 cut(s) 135, 788
SaqAI TTAA 4 cut(s) 26, 105, 525, 729
SatI GCNGC 5 cut(s) 219, 264, 648, 657, 780
Sau3AI GATC 4 cut(s) 252, 718, 751, 853
Sau96I GGNCC 3 cut(s) 185, 736, 878
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 227, 844
SfaNI GCATC 2 cut(s) 73, 760
SfuI TTCGAA 1 cut(s) 871
SinI GGWCC 2 cut(s) 185, 878
SmiMI CAYNNNNRTG 2 cut(s) 135, 788
SmlI CTYRAG 2 cut(s) 518, 533
SmoI CTYRAG 2 cut(s) 518, 533
Sse9I AATT 4 cut(s) 363, 499, 765, 847
SsiI CCGC 4 cut(s) 264, 285, 382, 445
StyD4I CCNGG 2 cut(s) 225, 842
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 2 cut(s) 40, 493
TaiI ACGT 2 cut(s) 118, 894
TaqI TCGA 5 cut(s) 357, 587, 708, 721, 871
TasI AATT 4 cut(s) 363, 499, 765, 847
TatI WGTACW 2 cut(s) 35, 835
TauI GCSGC 1 cut(s) 266
Tru1I TTAA 4 cut(s) 26, 105, 525, 729
Tru9I TTAA 4 cut(s) 26, 105, 525, 729
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 4 cut(s) 218, 647, 656, 779
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 4 cut(s) 11, 105, 375, 705
TspGWI ACGGA 2 cut(s) 466, 470
VpaK11BI GGWCC 2 cut(s) 185, 878
XapI RAATTY 3 cut(s) 363, 499, 847
XcmI CCANNNNNNNNNTGG 2 cut(s) 712, 790
XmiI GTMKAC 2 cut(s) 429, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.