RchiOBHm_Chr1g0357841

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016654
Physical Location & Seq
Forward (+)
50079349 .. 50081573
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1464 bp
ATGAAATACAACTGTTGCAACTATATGAGGAAACCGAGTGTTGTTATGAAGTTGGGAACATGGAGAGGAGTTCAGAGGTCAATTTTTATATTGGGTAGTTTTGCTCTTCTCATCTCAATTGTTACCTTATCATCCAAATTCTATTCTCTCAAATCATTTCATGTTACTGATACCTTCTGCAATTATGTAAATACTGATAGTCAAAGCAGGATTGACAGTCATGTAAAAGTCACTCTCGAAACTGTGATTCAGAAACTCCAACAGGAACTTAAAACGTTCAGAGATTCAACAGCAGATTCGTCGTCATCTGTGCATTTGTTAAAGCACAGTACGTTTCTTGCTGATATTTTGGGGCTGATCGAGTCGGTGCACGAATCTCTGGTGCAGAATGATGAGTCGACTCAGAAGTTAAGCAATGATGTCGAAGACATCCATCCTCTGTTAAAACCGAAGCAGCACTCTGATGAGCCTGCGGAGTATTTTCTAATAGAAGAGATTCGGAAGTATGTCAAGATCAAGCCGAACAGATTGGGAAAGCAGAATTTCATGGGAGCTAATGGGACTTTCACTAGCATTGGCCATGCTTGCTTTGCTATGAAGACAGAACTTGAAGAATACATGAACTACGACATTGGCGATATCTGCAATGACGATTGGAAGCTAGCTCAGAAGCTCATGGTTCATGGATGCGATCCACTTCCGAGGAGGAGGTGCTTTGCTCTGGCCCCGAAGATATATAGCAAGCCATTCCCAATCCATGAGTCCATATGGAAGCTCCCAGACAATAGAAATGTCCGGTGGAGTCAGTATCGGTGCAAGAACTTTACTTGCCTGGCAAGTAATTCCGCTCCCAAGGGGTTCTTCAAATGTGCAGATTGCTTCAACCTGTCACACCACGAAATGCCTAGATGGATGCAACAAGTAAGTCTAGACTCAAAATTGAATCTGACTGCAGATTTTCTTATCCCTGAAGTTCTTCAAATCAAGCCGGGAGAGATCAGAATCGGACTGGATTTTAGCGTAGGGACTGGGACTTTTGCAGCTAGGATGAGGGAGTTCAATGTCACCATTGTCTCGGCAACTATCAATCTCGGGGCACCTTTTAATGAAATGATAGCTCTTCGTGGACTCGTCCCTCTTTACTTGACAATCAATCAACGGCTGCCATTCTTCGACAACACTCTGGATTTGATTCACACCACAAGATTTCTGGATGGCTGGATTGATTTTGTGCTGCTAGACTTTGTCTTGTATGATTGGGATAGAGTTTTGAGGCCTGGGGGTTTGCTGTGGATTGATAGCTTCTTCTGTGTGAAGGAAGATTTGAATGCTTATTTGGAGTCCCTTAAGATGCTGAGGTACAAGAAGCACAAATGGGTTGTTGTTCCAAAGATTGATAAAGATGATGATAGAGAGGTTTTCTTTTCTGCTGTTCTAGAAAAGCCGCCCAGACCATTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

487

Amino Acids

56.29

Weight (kDa)

8.46

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 327 - 450 6.6e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1096
AccBSI CCGCTC 1 cut(s) 848
AccI GTMKAC 1 cut(s) 398
AciI CCGC 3 cut(s) 473, 846, 1445
AclI AACGTT 1 cut(s) 275
AclWI GGATC 1 cut(s) 686
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 2 cut(s) 137, 541
AcuI CTGAAG 1 cut(s) 990
AfaI GTAC 2 cut(s) 331, 1361
AflII CTTAAG 1 cut(s) 1346
AgsI TTSAA 8 cut(s) 288, 611, 865, 883, 943, 980, 1060, 1327
AjnI CCWGG 2 cut(s) 831, 1276
AjuI GAANNNNNNNTTGG 4 cut(s) 845, 877, 1319, 1351
AluBI AGCT 8 cut(s) 554, 661, 665, 673, 775, 1043, 1118, 1302
AluI AGCT 8 cut(s) 554, 661, 665, 673, 775, 1043, 1118, 1302
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 1078
Alw44I GTGCAC 1 cut(s) 368
AlwI GGATC 1 cut(s) 686
Ama87I CYCGRG 1 cut(s) 1091
AoxI GGCC 3 cut(s) 577, 723, 1274
ApaLI GTGCAC 1 cut(s) 368
ApeKI GCWGC 4 cut(s) 454, 1040, 1162, 1234
ApoI RAATTY 2 cut(s) 137, 541
Asp700I GAANNNNTTC 2 cut(s) 495, 975
AspS9I GGNCC 1 cut(s) 724
AsuC2I CCSGG 1 cut(s) 990
AsuHPI GGTGA 1 cut(s) 1057
AsuNHI GCTAGC 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 1091
BaeGI GKGCMC 2 cut(s) 372, 1099
BalI TGGCCA 1 cut(s) 579
BanI GGYRCC 1 cut(s) 1096
BbsI GAAGAC 2 cut(s) 432, 605
Bbv12I GWGCWC 1 cut(s) 372
BbvCI CCTCAGC 1 cut(s) 1355
BbvI GCAGC 4 cut(s) 466, 1052, 1149, 1221
BccI CCATC 3 cut(s) 441, 903, 1208
BceAI ACGGC 1 cut(s) 1175
BcgI CGANNNNNNTGC 4 cut(s) 282, 316, 403, 437
BciT130I CCWGG 2 cut(s) 833, 1278
BcnI CCSGG 1 cut(s) 990
BcoDI GTCTC 1 cut(s) 1078
BfaI CTAG 7 cut(s) 570, 662, 906, 929, 1044, 1238, 1436
BfmI CTRYAG 1 cut(s) 951
BfrI CTTAAG 1 cut(s) 1346
BisI GCNGC 5 cut(s) 455, 1041, 1163, 1235, 1445
BlsI GCNGC 5 cut(s) 456, 1042, 1164, 1236, 1446
Bme1390I CCNGG 3 cut(s) 833, 990, 1278
BmeT110I CYCGRG 1 cut(s) 1091
BmgT120I GGNCC 1 cut(s) 724
BmiI GGNNCC 2 cut(s) 726, 1098
BmrFI CCNGG 3 cut(s) 833, 990, 1278
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 3 cut(s) 677, 903, 1341
BmtI GCTAGC 1 cut(s) 665
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 2 cut(s) 432, 605
Bpu10I CCTNAGC 1 cut(s) 1355
BpuMI CCSGG 1 cut(s) 990
BsaBI GATNNNNATC 1 cut(s) 1001
BsaJI CCNNGG 3 cut(s) 701, 852, 1277
BsaWI WCCGGW 2 cut(s) 795, 1458
Bse1I ACTGG 2 cut(s) 1014, 1033
Bse3DI GCAATG 2 cut(s) 421, 652
Bse8I GATNNNNATC 1 cut(s) 1001
BseBI CCWGG 2 cut(s) 833, 1278
BseDI CCNNGG 3 cut(s) 701, 852, 1277
BseGI GGATG 7 cut(s) 131, 429, 433, 692, 918, 1053, 1219
BseJI GATNNNNATC 1 cut(s) 1001
BseMI GCAATG 2 cut(s) 421, 652
BseMII CTCAG 3 cut(s) 416, 680, 1346
BseNI ACTGG 2 cut(s) 1014, 1033
BseRI GAGGAG 3 cut(s) 81, 718, 721
BseSI GKGCMC 2 cut(s) 372, 1099
BseXI GCAGC 4 cut(s) 466, 1052, 1149, 1221
BsgI GTGCAG 2 cut(s) 404, 891
BshFI GGCC 3 cut(s) 579, 725, 1276
BshNI GGYRCC 1 cut(s) 1096
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 1091
BsiSI CCGG 3 cut(s) 796, 989, 1459
BslFI GGGAC 5 cut(s) 574, 1039, 1045, 1118, 1327
BsmAI GTCTC 1 cut(s) 1078
BsmFI GGGAC 5 cut(s) 574, 1039, 1045, 1118, 1327
BsmI GAATGC 1 cut(s) 1333
BsnI GGCC 3 cut(s) 579, 725, 1276
BsoBI CYCGRG 1 cut(s) 1091
Bsp1286I GDGCHC 2 cut(s) 372, 1099
Bsp143I GATC 4 cut(s) 357, 513, 691, 996
BspACI CCGC 3 cut(s) 473, 846, 1445
BspANI GGCC 3 cut(s) 579, 725, 1276
BspCNI CTCAG 3 cut(s) 415, 679, 1347
BspLI GGNNCC 2 cut(s) 726, 1098
BspMAI CTGCAG 1 cut(s) 955
BspOI GCTAGC 1 cut(s) 665
BspPI GGATC 1 cut(s) 686
BspQI GCTCTTC 2 cut(s) 111, 1125
BspT107I GGYRCC 1 cut(s) 1096
BspTI CTTAAG 1 cut(s) 1346
BsrBI CCGCTC 1 cut(s) 848
BsrDI GCAATG 2 cut(s) 421, 652
BsrI ACTGG 2 cut(s) 1014, 1033
BssECI CCNNGG 3 cut(s) 701, 852, 1277
BssMI GATC 4 cut(s) 357, 513, 691, 996
BssT1I CCWWGG 1 cut(s) 852
Bst2UI CCWGG 2 cut(s) 833, 1278
Bst4CI ACNGT 4 cut(s) 14, 218, 244, 329
Bst6I CTCTTC 3 cut(s) 111, 486, 1125
BstAFI CTTAAG 1 cut(s) 1346
BstC8I GCNNGC 4 cut(s) 471, 586, 663, 743
BstDEI CTNAG 3 cut(s) 402, 666, 1355
BstF5I GGATG 7 cut(s) 131, 429, 433, 692, 918, 1053, 1219
BstKTI GATC 4 cut(s) 360, 516, 694, 999
BstMAI GTCTC 1 cut(s) 1078
BstMBI GATC 4 cut(s) 357, 513, 691, 996
BstMWI GCNNNNNNNGC 3 cut(s) 585, 590, 642
BstNI CCWGG 2 cut(s) 833, 1278
BstSCI CCNGG 3 cut(s) 831, 988, 1276
BstSFI CTRYAG 1 cut(s) 951
BstSLI GKGCMC 2 cut(s) 372, 1099
BstV1I GCAGC 4 cut(s) 466, 1052, 1149, 1221
BstV2I GAAGAC 2 cut(s) 432, 605
BsuRI GGCC 3 cut(s) 579, 725, 1276
BtsCI GGATG 7 cut(s) 131, 429, 433, 692, 918, 1053, 1219
Cac8I GCNNGC 4 cut(s) 471, 586, 663, 743
Cfr13I GGNCC 1 cut(s) 724
Csp6I GTAC 2 cut(s) 330, 1360
CviAII CATG 9 cut(s) 60, 161, 221, 547, 581, 619, 676, 683, 758
CviQI GTAC 2 cut(s) 330, 1360
DdeI CTNAG 3 cut(s) 402, 666, 1355
DpnI GATC 4 cut(s) 359, 515, 693, 998
DpnII GATC 4 cut(s) 357, 513, 691, 996
EaeI YGGCCR 1 cut(s) 577
Eam1104I CTCTTC 3 cut(s) 111, 486, 1125
EarI CTCTTC 3 cut(s) 111, 486, 1125
Eco130I CCWWGG 1 cut(s) 852
Eco147I AGGCCT 1 cut(s) 1276
Eco32I GATATC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 990
Eco88I CYCGRG 1 cut(s) 1091
EcoRII CCWGG 2 cut(s) 831, 1276
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 852
FaeI CATG 9 cut(s) 63, 164, 224, 550, 584, 622, 679, 686, 761
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FaqI GGGAC 5 cut(s) 574, 1039, 1045, 1118, 1327
FatI CATG 9 cut(s) 59, 160, 220, 546, 580, 618, 675, 682, 757
FauNDI CATATG 1 cut(s) 767
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 5 cut(s) 455, 1041, 1163, 1235, 1445
FokI GGATG 7 cut(s) 118, 416, 420, 699, 925, 1060, 1226
Fsp4HI GCNGC 5 cut(s) 455, 1041, 1163, 1235, 1445
FspBI CTAG 7 cut(s) 570, 662, 906, 929, 1044, 1238, 1436
GluI GCNGC 5 cut(s) 455, 1041, 1163, 1235, 1445
HaeIII GGCC 3 cut(s) 579, 725, 1276
HapII CCGG 3 cut(s) 796, 989, 1459
Hin1II CATG 9 cut(s) 63, 164, 224, 550, 584, 622, 679, 686, 761
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HpaII CCGG 3 cut(s) 796, 989, 1459
HphI GGTGA 1 cut(s) 1057
Hpy166II GTNNAC 3 cut(s) 370, 399, 1127
Hpy188III TCNNGA 6 cut(s) 236, 511, 929, 1184, 1211, 1436
Hpy8I GTNNAC 3 cut(s) 370, 399, 1127
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 2 cut(s) 184, 1309
HpyCH4III ACNGT 4 cut(s) 14, 218, 244, 329
HpyCH4IV ACGT 2 cut(s) 275, 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 585, 590, 642
HpyF3I CTNAG 3 cut(s) 402, 666, 1355
HpySE526I ACGT 2 cut(s) 275, 332
Hsp92II CATG 9 cut(s) 63, 164, 224, 550, 584, 622, 679, 686, 761
Kzo9I GATC 4 cut(s) 357, 513, 691, 996
LguI GCTCTTC 2 cut(s) 111, 1125
LmnI GCTCC 3 cut(s) 551, 780, 853
Lsp1109I GCAGC 4 cut(s) 466, 1052, 1149, 1221
LweI GCATC 3 cut(s) 677, 903, 1341
MaeI CTAG 7 cut(s) 570, 662, 906, 929, 1044, 1238, 1436
MaeII ACGT 2 cut(s) 275, 332
MaeIII GTNAC 5 cut(s) 121, 163, 229, 888, 1063
MalI GATC 4 cut(s) 359, 515, 693, 998
MbiI CCGCTC 1 cut(s) 848
MboI GATC 4 cut(s) 357, 513, 691, 996
MfeI CAATTG 1 cut(s) 117
MhlI GDGCHC 2 cut(s) 372, 1099
MlsI TGGCCA 1 cut(s) 579
MluCI AATT 7 cut(s) 81, 117, 137, 181, 541, 841, 938
MluNI TGGCCA 1 cut(s) 579
MlyI GAGTC 8 cut(s) 371, 394, 404, 770, 811, 926, 1122, 1349
MmeI TCCRAC 1 cut(s) 283
Mox20I TGGCCA 1 cut(s) 579
MroXI GAANNNNTTC 2 cut(s) 495, 975
MscI TGGCCA 1 cut(s) 579
MseI TTAA 6 cut(s) 270, 320, 410, 443, 1104, 1347
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 579
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 3 cut(s) 796, 989, 1459
MspR9I CCNGG 3 cut(s) 833, 990, 1278
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 1333
MvaI CCWGG 2 cut(s) 833, 1278
MwoI GCNNNNNNNGC 3 cut(s) 585, 590, 642
NciI CCSGG 1 cut(s) 990
NdeI CATATG 1 cut(s) 767
NdeII GATC 4 cut(s) 357, 513, 691, 996
NheI GCTAGC 1 cut(s) 661
NlaIII CATG 9 cut(s) 63, 164, 224, 550, 584, 622, 679, 686, 761
NlaIV GGNNCC 2 cut(s) 726, 1098
NmeAIII GCCGAG 1 cut(s) 1055
NmuCI GTSAC 3 cut(s) 229, 888, 1063
PceI AGGCCT 1 cut(s) 1276
PciSI GCTCTTC 2 cut(s) 111, 1125
PcsI WCGNNNNNNNCGW 1 cut(s) 633
PctI GAATGC 1 cut(s) 1333
PdmI GAANNNNTTC 2 cut(s) 495, 975
PfeI GAWTC 8 cut(s) 247, 284, 296, 374, 496, 943, 1002, 1192
PflFI GACNNNGTC 1 cut(s) 1244
PkrI GCNGC 5 cut(s) 456, 1042, 1164, 1236, 1446
PleI GAGTC 8 cut(s) 370, 394, 403, 769, 810, 926, 1122, 1348
PpsI GAGTC 8 cut(s) 370, 394, 403, 769, 810, 926, 1122, 1348
Psp1406I AACGTT 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 831, 1276
PspGI CCWGG 2 cut(s) 831, 1276
PspN4I GGNNCC 2 cut(s) 726, 1098
PspPI GGNCC 1 cut(s) 724
PstI CTGCAG 1 cut(s) 955
PsyI GACNNNGTC 1 cut(s) 1244
RsaI GTAC 2 cut(s) 331, 1361
RsaNI GTAC 2 cut(s) 330, 1360
RseI CAYNNNNRTG 1 cut(s) 462
SalI GTCGAC 1 cut(s) 397
SapI GCTCTTC 2 cut(s) 111, 1125
SaqAI TTAA 6 cut(s) 270, 320, 410, 443, 1104, 1347
SatI GCNGC 5 cut(s) 455, 1041, 1163, 1235, 1445
Sau3AI GATC 4 cut(s) 357, 513, 691, 996
Sau96I GGNCC 1 cut(s) 724
SchI GAGTC 8 cut(s) 371, 394, 404, 770, 811, 926, 1122, 1349
ScrFI CCNGG 3 cut(s) 833, 990, 1278
SduI GDGCHC 2 cut(s) 372, 1099
SfaNI GCATC 3 cut(s) 677, 903, 1341
SfcI CTRYAG 1 cut(s) 951
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 1 cut(s) 1346
SmoI CTYRAG 1 cut(s) 1346
Sse9I AATT 7 cut(s) 81, 117, 137, 181, 541, 841, 938
SseBI AGGCCT 1 cut(s) 1276
SsiI CCGC 3 cut(s) 473, 846, 1445
SspMI CTAG 7 cut(s) 570, 662, 906, 929, 1044, 1238, 1436
StuI AGGCCT 1 cut(s) 1276
StyD4I CCNGG 3 cut(s) 831, 988, 1276
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 4 cut(s) 14, 218, 244, 329
TaiI ACGT 2 cut(s) 278, 335
TaqI TCGA 5 cut(s) 237, 360, 398, 423, 1173
TasI AATT 7 cut(s) 81, 117, 137, 181, 541, 841, 938
TauI GCSGC 1 cut(s) 1447
TfiI GAWTC 8 cut(s) 247, 284, 296, 374, 496, 943, 1002, 1192
Tru1I TTAA 6 cut(s) 270, 320, 410, 443, 1104, 1347
Tru9I TTAA 6 cut(s) 270, 320, 410, 443, 1104, 1347
TseFI GTSAC 3 cut(s) 229, 888, 1063
TseI GCWGC 4 cut(s) 454, 1040, 1162, 1234
Tsp45I GTSAC 3 cut(s) 229, 888, 1063
TspDTI ATGAA 8 cut(s) 17, 62, 149, 535, 611, 635, 671, 1122
Tth111I GACNNNGTC 1 cut(s) 1244
Vha464I CTTAAG 1 cut(s) 1346
VneI GTGCAC 1 cut(s) 368
XapI RAATTY 2 cut(s) 137, 541
XbaI TCTAGA 2 cut(s) 928, 1435
XcmI CCANNNNNNNNNTGG 1 cut(s) 1207
XmiI GTMKAC 1 cut(s) 398
XmnI GAANNNNTTC 2 cut(s) 495, 975
XspI CTAG 7 cut(s) 570, 662, 906, 929, 1044, 1238, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.