RchiOBHm_Chr1g0361921

of pyruvate dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0009625
Physical Location & Seq
Forward (+)
53685180 .. 53693119
7940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1899 bp
ATGGCGCTCTCACGGCTTCGACACCCTGTGATCTCACGCGCCCCTTCTCTCTTCCGAGCTCGCCTTCTCTCCTCGTCCACCAGATCACTCACTCGAAGTTCCAGTGTGCAGAATTTGACTGTGGGAGGTGATAGTGCTCTTTTAAGGCCTGCATCTTTGTCCATGCTCACCGGAGTCCAGTATAAATTTTCATACCTCAAGCCTTGGACGGGTGTCAAGCATTACTCAACTGCCGATCCTTTGTATGCTGTCCTTGATATGCCTGCTTTGTCTCCTACAATGAGTCAAGGTAACATTGCGAAATGGAGGAAGAAAGAGGGAGATAAGATTGAAGTGGGTGATGTACTATGTGAAATTGAAACTGACAAAGCTACTCTTGAATTTGAAAGTCTTGAAGAAGGGTTTCTAGCCAAGATACTGGTACCTGAAGGTTCAAAGGATGTGCCTGTGGGACAACCTATTGCAGTAACGGTCGAGGATAAAGATGAAATCGAAAACATCCCTGCTAATATTGTGGGTGGATCTGAGGTGAAAGAGGACATACCACAACATCAGAAAGATGAGGCACAAGATACAAGTTCTGTGGGGATAAACACGGCAGACCTTCCTCCTCACATTGTTGTTGAAATGCCAGCTTTGTCTCCAACAATGAGCCAAGGAAACATTGCTGTGTGGAGAAAGAAAGAAGGAGATAAGATAGAAGTTGGTGATGTGTTGTGTGAGATAGAGACGGACAAAGCCACCCTTGAATTTGAATGCCTTGAAGAGGGATACTTGGCTAAAATACTAGCACCTGAAGGTTCAAAGGATGTGGCTGTTGGCCAACCAATTGCAGTAACAGTTGAGGATGCAGCTGATTTGGAAACTGTAAAGAATGGTGTCAGTGTTGGTTCAAGTGTCAAAAAGGAAAAACCAATTCATCATGATACTAAACATGAAAGTGGAGCAGTAAAAACAACCAGTGTCAAAAGGATCAGTCCAGCAGCTAAGAATCTAATTCTAGAACACGGATTAGATACATCGTCATTGAGGGCATCAGGTGCTCATGGTACGCTACTGAAAGGGGATGTTTTAGCGGCAATCAAGTCAGGAACTGGATCTTCAAAAGTTTCATCTTCTAAAGAGAAGGCACCTTCATCTTCTCAAGCACATATAAAGATAGCTCCAGCATCTCCAGAGTCAAGGTCTTTACAGCAGACAGATTTTGAAGAGTTTCCCAACAGTCAAATTCGCAAGGTGATAGCTAGTAGGTTGCTGGAATCAAAACAAAACATACCACACTTGTATTTATCCTCAGATGTTATCCTGGATCCTCTTCTTTCTCTTAGAAAGGATCTTAAAGAGCAGCATAATGTTAAAGTTTCAGTGAATGACATTGTCATTAGAGCTGTAGCTATTGCACTGAGAAATGTACCGGAAGCAAATGCTTACTGGGATGCTGAAAAGGGAGAAGTGATTCTTTGTGATTCTGTTGATATATCAATTGCTGTCGCTACCGACAAGGGTCTGATGACTCCAATTGTGAAAAATGCGGATCAGAAAACAATATCAGCAATCTCCTCAGAGGTCAAGGAACTAGCTGAGAAGGCACGGGCAGGAAAGCTTAAGCCAAGTGAATTCCAAGGAGGGACTTTCAGCATTTCAAACTTGGGGATGTTTCCGGTGGATCATTTCTGTGCAATTATAAATCCACCTCAGGCCAGTATCCTTGCTGTAGGCAGAGGCAACAAAGTTGTTGAACCAGTAATTGGAAGTGATGGAATTGAAAGGCCAGCAGTTGTCACAAAAATGAACTTGACGTTATCGGCGGATCATCGTGTTTTTGATGGCAAAGTTGGAGGTACATTCCTCTCAGCTTTACAATCAAACTTCAGTGACATCCGAAGACTTCTTTTGTAA

Protein Analysis

632

Amino Acids

67.91

Weight (kDa)

5.95

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 85 - 156 2.1e-18 Biotin-requiring enzyme
Biotin_lipoyl PF00364 208 - 279 5.1e-17 Biotin-requiring enzyme
E3_binding PF02817 324 - 358 1.3e-07 e3 binding domain
2-oxoacid_dh PF00198 401 - 631 1.1e-75 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1685
Acc65I GGTACC 1 cut(s) 421
AccB1I GGYRCC 2 cut(s) 421, 1129
AccB7I CCANNNNNTGG 1 cut(s) 1748
AccII CGCG 1 cut(s) 39
AciI CCGC 3 cut(s) 1076, 1532, 1808
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 6 cut(s) 112, 185, 380, 749, 1227, 1616
AcuI CTGAAG 3 cut(s) 447, 816, 1855
AdeI CACNNNGTG 1 cut(s) 28
AfaI GTAC 5 cut(s) 345, 423, 1051, 1413, 1843
AfiI CCNNNNNNNGG 3 cut(s) 209, 766, 1748
AflII CTTAAG 1 cut(s) 1604
AjnI CCWGG 1 cut(s) 1305
AjuI GAANNNNNNNTTGG 2 cut(s) 1731, 1763
Alw21I GWGCWC 3 cut(s) 61, 139, 1045
Alw26I GTCTC 3 cut(s) 276, 645, 722
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 4 cut(s) 146, 820, 1698, 1769
ApeKI GCWGC 3 cut(s) 851, 983, 1345
ApoI RAATTY 6 cut(s) 112, 185, 380, 749, 1227, 1616
Asp700I GAANNNNTTC 3 cut(s) 402, 1212, 1455
Asp718I GGTACC 1 cut(s) 421
AspLEI GCGC 2 cut(s) 7, 41
AsuHPI GGTGA 6 cut(s) 140, 160, 350, 541, 719, 1249
AxyI CCTNAGG 1 cut(s) 1695
BalI TGGCCA 1 cut(s) 822
BamHI GGATCC 1 cut(s) 1309
BanI GGYRCC 2 cut(s) 421, 1129
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 1891
Bbv12I GWGCWC 3 cut(s) 61, 139, 1045
BbvI GCAGC 3 cut(s) 863, 995, 1357
BccI CCATC 2 cut(s) 1751, 1820
BceAI ACGGC 2 cut(s) 29, 612
BciT130I CCWGG 1 cut(s) 1307
BciVI GTATCC 2 cut(s) 764, 1715
BcoDI GTCTC 3 cut(s) 276, 645, 722
BfaI CTAG 5 cut(s) 407, 788, 1001, 1245, 1577
BfmI CTRYAG 2 cut(s) 1389, 1713
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 1604
BfuI GTATCC 2 cut(s) 764, 1715
BisI GCNGC 4 cut(s) 852, 984, 1077, 1346
BlsI GCNGC 4 cut(s) 853, 985, 1078, 1347
Bme1390I CCNGG 1 cut(s) 1307
BmiI GGNNCC 3 cut(s) 423, 1131, 1311
BmrFI CCNGG 1 cut(s) 1307
BmrI ACTGGG 1 cut(s) 1441
BmsI GCATC 5 cut(s) 161, 838, 1043, 1178, 1426
BmuI ACTGGG 1 cut(s) 1441
BoxI GACNNNNGTC 2 cut(s) 212, 1503
BpiI GAAGAC 1 cut(s) 1891
BpmI CTGGAG 2 cut(s) 1149, 1158
BpuEI CTTGAG 2 cut(s) 182, 1128
BsaJI CCNNGG 3 cut(s) 203, 655, 1621
BsaWI WCCGGW 3 cut(s) 170, 1414, 1660
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 766, 1748
Bse1I ACTGG 8 cut(s) 102, 178, 423, 960, 1099, 1436, 1701, 1742
Bse21I CCTNAGG 1 cut(s) 1695
Bse3DI GCAATG 2 cut(s) 294, 663
BseBI CCWGG 1 cut(s) 1307
BseDI CCNNGG 3 cut(s) 203, 655, 1621
BseGI GGATG 8 cut(s) 445, 498, 814, 853, 1072, 1441, 1659, 1878
BseLI CCNNNNNNNGG 3 cut(s) 209, 766, 1748
BseMI GCAATG 2 cut(s) 294, 663
BseMII CTCAG 7 cut(s) 516, 1308, 1394, 1572, 1575, 1709, 1866
BseNI ACTGG 8 cut(s) 102, 178, 423, 960, 1099, 1436, 1701, 1742
BseRI GAGGAG 3 cut(s) 61, 600, 1549
BseXI GCAGC 3 cut(s) 863, 995, 1357
BsgI GTGCAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 4 cut(s) 148, 822, 1700, 1771
BshNI GGYRCC 2 cut(s) 421, 1129
BsiEI CGRYCG 1 cut(s) 474
BsiHKAI GWGCWC 3 cut(s) 61, 139, 1045
BsiSI CCGG 3 cut(s) 171, 1415, 1661
BslFI GGGAC 2 cut(s) 465, 1642
BslI CCNNNNNNNGG 3 cut(s) 209, 766, 1748
BsmAI GTCTC 3 cut(s) 276, 645, 722
BsmBI CGTCTC 1 cut(s) 722
BsmFI GGGAC 2 cut(s) 465, 1642
BsmI GAATGC 1 cut(s) 761
BsnI GGCC 4 cut(s) 148, 822, 1700, 1771
Bsp1286I GDGCHC 3 cut(s) 61, 139, 1045
BspACI CCGC 3 cut(s) 1076, 1532, 1808
BspANI GGCC 4 cut(s) 148, 822, 1700, 1771
BspCNI CTCAG 7 cut(s) 517, 1307, 1395, 1573, 1574, 1708, 1865
BspFNI CGCG 1 cut(s) 39
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 3 cut(s) 423, 1131, 1311
BspT107I GGYRCC 2 cut(s) 421, 1129
BspTI CTTAAG 1 cut(s) 1604
BsrDI GCAATG 2 cut(s) 294, 663
BsrI ACTGG 8 cut(s) 102, 178, 423, 960, 1099, 1436, 1701, 1742
BssECI CCNNGG 3 cut(s) 203, 655, 1621
BssT1I CCWWGG 3 cut(s) 203, 655, 1621
Bst2UI CCWGG 1 cut(s) 1307
Bst4CI ACNGT 5 cut(s) 121, 472, 841, 868, 1223
Bst6I CTCTTC 4 cut(s) 56, 759, 1203, 1320
BstAFI CTTAAG 1 cut(s) 1604
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstC8I GCNNGC 5 cut(s) 61, 150, 264, 633, 1773
BstDEI CTNAG 9 cut(s) 525, 987, 1294, 1325, 1403, 1561, 1581, 1695, 1852
BstENI CCTNNNNNAGG 1 cut(s) 764
BstF5I GGATG 8 cut(s) 445, 498, 814, 853, 1072, 1441, 1659, 1878
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 41
BstMAI GTCTC 3 cut(s) 276, 645, 722
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 3 cut(s) 13, 1040, 1586
BstNI CCWGG 1 cut(s) 1307
BstPAI GACNNNNGTC 2 cut(s) 212, 1503
BstSCI CCNGG 1 cut(s) 1305
BstSFI CTRYAG 2 cut(s) 1389, 1713
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 863, 995, 1357
BstV2I GAAGAC 1 cut(s) 1891
BstX2I RGATCY 4 cut(s) 521, 1097, 1309, 1333
BstXI CCANNNNNNTGG 1 cut(s) 418
BstYI RGATCY 4 cut(s) 521, 1097, 1309, 1333
Bsu36I CCTNAGG 1 cut(s) 1695
BsuI GTATCC 2 cut(s) 764, 1715
BsuRI GGCC 4 cut(s) 148, 822, 1700, 1771
BtsCI GGATG 8 cut(s) 445, 498, 814, 853, 1072, 1441, 1659, 1878
BtsIMutI CAGTG 6 cut(s) 109, 889, 967, 1371, 1400, 1879
Cac8I GCNNGC 5 cut(s) 61, 150, 264, 633, 1773
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 1 cut(s) 922
CfoI GCGC 2 cut(s) 7, 41
Csp6I GTAC 5 cut(s) 344, 422, 1050, 1412, 1842
CspCI CAANNNNNGTGG 4 cut(s) 564, 599, 792, 827
CviAII CATG 4 cut(s) 163, 923, 935, 1046
CviQI GTAC 5 cut(s) 344, 422, 1050, 1412, 1842
DdeI CTNAG 9 cut(s) 525, 987, 1294, 1325, 1403, 1561, 1581, 1695, 1852
DraIII CACNNNGTG 1 cut(s) 28
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 4 cut(s) 56, 759, 1203, 1320
EarI CTCTTC 4 cut(s) 56, 759, 1203, 1320
EciI GGCGGA 1 cut(s) 1823
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 3 cut(s) 203, 655, 1621
Eco147I AGGCCT 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 3 cut(s) 447, 816, 1855
Eco81I CCTNAGG 1 cut(s) 1695
EcoICRI GAGCTC 1 cut(s) 59
EcoNI CCTNNNNNAGG 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 1616
EcoRII CCWGG 1 cut(s) 1305
EcoT14I CCWWGG 3 cut(s) 203, 655, 1621
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 3 cut(s) 203, 655, 1621
Esp3I CGTCTC 1 cut(s) 722
FaeI CATG 4 cut(s) 166, 926, 938, 1049
FalI AAGNNNNNCTT 6 cut(s) 360, 392, 729, 761, 1443, 1475
FaqI GGGAC 2 cut(s) 465, 1642
FatI CATG 4 cut(s) 162, 922, 934, 1045
Fnu4HI GCNGC 4 cut(s) 852, 984, 1077, 1346
FokI GGATG 8 cut(s) 452, 485, 821, 860, 1079, 1448, 1666, 1865
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 4 cut(s) 852, 984, 1077, 1346
FspBI CTAG 5 cut(s) 407, 788, 1001, 1245, 1577
GlaI GCGC 2 cut(s) 6, 40
GluI GCNGC 4 cut(s) 852, 984, 1077, 1346
GsuI CTGGAG 2 cut(s) 1149, 1158
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 148, 822, 1700, 1771
HapII CCGG 3 cut(s) 171, 1415, 1661
HhaI GCGC 2 cut(s) 7, 41
Hin1II CATG 4 cut(s) 166, 926, 938, 1049
Hin6I GCGC 2 cut(s) 5, 39
HinP1I GCGC 2 cut(s) 5, 39
HindIII AAGCTT 1 cut(s) 1601
HinfI GANTC 8 cut(s) 174, 283, 991, 1178, 1259, 1456, 1466, 1513
HpaII CCGG 3 cut(s) 171, 1415, 1661
HphI GGTGA 6 cut(s) 140, 160, 350, 541, 719, 1249
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 8 cut(s) 56, 526, 555, 1297, 1509, 1539, 1564, 1883
Hpy188III TCNNGA 6 cut(s) 377, 392, 923, 1001, 1089, 1175
Hpy8I GTNNAC 1 cut(s) 78
HpyCH4III ACNGT 5 cut(s) 121, 472, 841, 868, 1223
HpyCH4IV ACGT 1 cut(s) 1799
HpyCH4V TGCA 7 cut(s) 109, 152, 464, 833, 851, 1400, 1679
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 1040, 1586
HpyF3I CTNAG 9 cut(s) 525, 987, 1294, 1325, 1403, 1561, 1581, 1695, 1852
HpySE526I ACGT 1 cut(s) 1799
Hsp92II CATG 4 cut(s) 166, 926, 938, 1049
HspAI GCGC 2 cut(s) 5, 39
KpnI GGTACC 1 cut(s) 425
LmnI GCTCC 2 cut(s) 944, 1168
Lsp1109I GCAGC 3 cut(s) 863, 995, 1357
LweI GCATC 5 cut(s) 161, 838, 1043, 1178, 1426
MaeI CTAG 5 cut(s) 407, 788, 1001, 1245, 1577
MaeII ACGT 1 cut(s) 1799
MaeIII GTNAC 5 cut(s) 290, 466, 835, 1780, 1874
MfeI CAATTG 3 cut(s) 828, 1482, 1518
MflI RGATCY 4 cut(s) 521, 1097, 1309, 1333
MhlI GDGCHC 3 cut(s) 61, 139, 1045
MlsI TGGCCA 1 cut(s) 822
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 4 cut(s) 183, 292, 1187, 1507
MmeI TCCRAC 2 cut(s) 668, 1816
Mox20I TGGCCA 1 cut(s) 822
MroXI GAANNNNTTC 3 cut(s) 402, 1212, 1455
MscI TGGCCA 1 cut(s) 822
MseI TTAA 4 cut(s) 143, 1338, 1356, 1605
MslI CAYNNNNRTG 4 cut(s) 668, 939, 1674, 1787
Msp20I TGGCCA 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 854
MspCI CTTAAG 1 cut(s) 1604
MspI CCGG 3 cut(s) 171, 1415, 1661
MspR9I CCNGG 1 cut(s) 1307
MunI CAATTG 3 cut(s) 828, 1482, 1518
Mva1269I GAATGC 1 cut(s) 761
MvaI CCWGG 1 cut(s) 1307
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 3 cut(s) 13, 1040, 1586
NlaIII CATG 4 cut(s) 166, 926, 938, 1049
NlaIV GGNNCC 3 cut(s) 423, 1131, 1311
NmuCI GTSAC 2 cut(s) 1780, 1874
PagI TCATGA 1 cut(s) 922
PceI AGGCCT 1 cut(s) 148
PctI GAATGC 1 cut(s) 761
PdmI GAANNNNTTC 3 cut(s) 402, 1212, 1455
PfeI GAWTC 4 cut(s) 991, 1259, 1456, 1466
PflFI GACNNNGTC 1 cut(s) 1376
PflMI CCANNNNNTGG 1 cut(s) 1748
PfoI TCCNGGA 1 cut(s) 1305
PkrI GCNGC 4 cut(s) 853, 985, 1078, 1347
PleI GAGTC 4 cut(s) 182, 291, 1186, 1507
PpsI GAGTC 4 cut(s) 182, 291, 1186, 1507
PshAI GACNNNNGTC 2 cut(s) 212, 1503
PsiI TTATAA 1 cut(s) 1685
Psp124BI GAGCTC 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 1305
PspGI CCWGG 1 cut(s) 1305
PspN4I GGNNCC 3 cut(s) 423, 1131, 1311
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 4 cut(s) 521, 1097, 1309, 1333
PsyI GACNNNGTC 1 cut(s) 1376
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 5 cut(s) 345, 423, 1051, 1413, 1843
RsaNI GTAC 5 cut(s) 344, 422, 1050, 1412, 1842
RseI CAYNNNNRTG 4 cut(s) 668, 939, 1674, 1787
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 4 cut(s) 143, 1338, 1356, 1605
SatI GCNGC 4 cut(s) 852, 984, 1077, 1346
SchI GAGTC 4 cut(s) 183, 292, 1187, 1507
ScrFI CCNGG 1 cut(s) 1307
SduI GDGCHC 3 cut(s) 61, 139, 1045
SfaNI GCATC 5 cut(s) 161, 838, 1043, 1178, 1426
SfcI CTRYAG 2 cut(s) 1389, 1713
SmiMI CAYNNNNRTG 4 cut(s) 668, 939, 1674, 1787
SmlI CTYRAG 3 cut(s) 197, 1143, 1604
SmoI CTYRAG 3 cut(s) 197, 1143, 1604
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 3 cut(s) 1076, 1532, 1808
SspI AATATT 1 cut(s) 511
SspMI CTAG 5 cut(s) 407, 788, 1001, 1245, 1577
SstI GAGCTC 1 cut(s) 61
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 1 cut(s) 1305
StyI CCWWGG 3 cut(s) 203, 655, 1621
TaaI ACNGT 5 cut(s) 121, 472, 841, 868, 1223
TaiI ACGT 1 cut(s) 1802
TaqI TCGA 4 cut(s) 19, 94, 474, 492
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 1079
TfiI GAWTC 4 cut(s) 991, 1259, 1456, 1466
Tru1I TTAA 4 cut(s) 143, 1338, 1356, 1605
Tru9I TTAA 4 cut(s) 143, 1338, 1356, 1605
TscAI CASTG 6 cut(s) 109, 889, 967, 1371, 1407, 1879
TseFI GTSAC 2 cut(s) 1780, 1874
TseI GCWGC 3 cut(s) 851, 983, 1345
Tsp45I GTSAC 2 cut(s) 1780, 1874
TspDTI ATGAA 7 cut(s) 180, 501, 908, 951, 1101, 1125, 1805
TspGWI ACGGA 2 cut(s) 746, 1023
TspRI CASTG 6 cut(s) 109, 889, 967, 1371, 1407, 1879
Tth111I GACNNNGTC 1 cut(s) 1376
Van91I CCANNNNNTGG 1 cut(s) 1748
Vha464I CTTAAG 1 cut(s) 1604
XagI CCTNNNNNAGG 1 cut(s) 764
XapI RAATTY 6 cut(s) 112, 185, 380, 749, 1227, 1616
XbaI TCTAGA 1 cut(s) 1000
XmnI GAANNNNTTC 3 cut(s) 402, 1212, 1455
XspI CTAG 5 cut(s) 407, 788, 1001, 1245, 1577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.