RchiOBHm_Chr1g0369171

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011593
Physical Location & Seq
Forward (+)
59091087 .. 59093364
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGCTCAATCACGTTCATACATCCATTAAGCCTATTATATACATGAAACGAAATGCAACAAATGGCTATATTATGATCAAGGCCAATGGTGGGTTGAATCAAATGAAGCTTGGGATAAGTGATATGGTTGCCGTAGCAAAACTTATGAATGCCACTCTGGTTCTCCCTTTTTTAGATCATAAATCCTTTTGGACCGATCCAAGTGATTTTAAGGACATTTTCAACTGGAAGAATTTCATGGAAGTCTTAAAAGATGATATCATGGTTATAGAGTCACTCCCACCGGAGCTTGCAGACCTGAAGCCTCTCCCCAGGGCTCCAGTTTCCTGGTCCAAGGCTAGTTACTACAGAGGATATATGTCTTCGTTATTGAAGCAGCACAAAGTGCTCAAATTCACGTACACAGATTCCAGACTTGCTAATGATCTTGGTGGTTCATTCCAAAAGCTACGCTGCCGTGCAATGTTTGAGGCACTGAGATTCTCACCTGAAATCGAACAACTAGCATCAAAGTTGGTTAACAGATTAAAGAACAACAATAGTAGATACATTGGTCTCCATTTAAGATATGAAAAGGACATGCTTTCTTTCACAGGCTGCAGTCACAATCTGACCAAAGACGAAGATAGAGAACTCGCAAAAATAAGGAAACAAACATCACACTGGAAGCATAAGACGATTAATAGCACCAATCATAGACTCCAAGGGGTATGCCCCATGACCCCAAGAGAGGTTGCTGTGTTTCTTGAAGCAATTGGGTTTCCTTTGGATACAAAAGTTTATGTAGCCGCTGGGAAGATCTACGGGAGAGATGGGATTAGACCTCTGAAAGATAAGTACCCCAATTTATTCTTCCACTCAAATTTAGCGACTGAAGTAGAGTTGCAACCTTTCAAGAACTTGCAGAACCAGCTTGCTGCCTTGGATTACATTTTGGCTTTGGAGAGTGATGTGTTCATTTATTCTTTTGATGGAAATATGGCTAAAGCAATAAAAGGGCATCGACGATTTGAAGGGTTCCGGAAGACGATCAACCCCAACAAACAACAATTTGTGACATTGATTGATAGGTTGGACAACGGAATGATATCTTGGGAAAAATTCTCATCCAAGGTGAAGAAGTTGCATGAAAATCGGATCGGAGCACCATACTCCAGAGTAGCCAGAGGTGACCACAAACTTGAGGAGAACTTTTACGCAAACCCTTTTCCGGGTTGCATCTGTGAAAATTCCGGAGGCTCATCAACAGTAGCCAAGCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

421

Amino Acids

48.24

Weight (kDa)

9.56

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 23 - 337 1.4e-96 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 1020, 1232
AciI CCGC 1 cut(s) 789
AclWI GGATC 2 cut(s) 191, 1145
AcsI RAATTY 5 cut(s) 232, 392, 862, 1100, 1228
AcuI CTGAAG 2 cut(s) 320, 894
AdeI CACNNNGTG 1 cut(s) 385
AfaI GTAC 2 cut(s) 401, 839
AfiI CCNNNNNNNGG 4 cut(s) 90, 730, 1210, 1211
AgsI TTSAA 6 cut(s) 97, 223, 373, 749, 895, 1013
AjnI CCWGG 2 cut(s) 311, 326
AjuI GAANNNNNNNTTGG 4 cut(s) 836, 868, 1075, 1107
AluBI AGCT 4 cut(s) 109, 289, 448, 913
AluI AGCT 4 cut(s) 109, 289, 448, 913
Alw21I GWGCWC 2 cut(s) 390, 1147
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 2 cut(s) 191, 1145
Aor13HI TCCGGA 2 cut(s) 1020, 1232
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 4 cut(s) 376, 453, 597, 917
ApoI RAATTY 5 cut(s) 232, 392, 862, 1100, 1228
AseI ATTAAT 1 cut(s) 681
Asp700I GAANNNNTTC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 192, 330
AsuC2I CCSGG 1 cut(s) 1212
AsuHPI GGTGA 3 cut(s) 477, 1126, 1181
AvaII GGWCC 2 cut(s) 192, 330
BaeI ACNNNNGTAYC 4 cut(s) 538, 571, 762, 795
BanII GRGCYC 1 cut(s) 319
BbsI GAAGAC 2 cut(s) 354, 1031
Bbv12I GWGCWC 2 cut(s) 390, 1147
BbvI GCAGC 4 cut(s) 388, 440, 584, 904
BccI CCATC 2 cut(s) 806, 965
BceAI ACGGC 2 cut(s) 116, 441
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 2 cut(s) 313, 328
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 1 cut(s) 75
BcnI CCSGG 1 cut(s) 1212
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 2 cut(s) 339, 503
BfmI CTRYAG 2 cut(s) 346, 598
BfuI GTATCC 1 cut(s) 763
BglII AGATCT 1 cut(s) 798
BisI GCNGC 5 cut(s) 377, 454, 598, 789, 918
BlsI GCNGC 5 cut(s) 378, 455, 599, 790, 919
Bme1390I CCNGG 3 cut(s) 313, 328, 1212
Bme18I GGWCC 2 cut(s) 192, 330
BmgT120I GGNCC 2 cut(s) 192, 330
BmiI GGNNCC 2 cut(s) 318, 1019
BmrFI CCNGG 3 cut(s) 313, 328, 1212
BmsI GCATC 3 cut(s) 515, 1009, 1227
BpiI GAAGAC 2 cut(s) 354, 1031
BpmI CTGGAG 2 cut(s) 303, 1138
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 6 cut(s) 311, 312, 333, 703, 921, 1110
BsaWI WCCGGW 3 cut(s) 283, 1020, 1232
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 730, 1210, 1211
Bse1I ACTGG 3 cut(s) 230, 320, 668
Bse3DI GCAATG 1 cut(s) 468
BseAI TCCGGA 2 cut(s) 1020, 1232
BseBI CCWGG 2 cut(s) 313, 328
BseDI CCNNGG 6 cut(s) 311, 312, 333, 703, 921, 1110
BseGI GGATG 2 cut(s) 20, 1106
BseLI CCNNNNNNNGG 4 cut(s) 90, 730, 1210, 1211
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 467
BseNI ACTGG 3 cut(s) 230, 320, 668
BseRI GAGGAG 1 cut(s) 1199
BseXI GCAGC 4 cut(s) 388, 440, 584, 904
BseYI CCCAGC 1 cut(s) 791
BshFI GGCC 1 cut(s) 83
BsiHKAI GWGCWC 2 cut(s) 390, 1147
BsiSI CCGG 4 cut(s) 284, 1021, 1211, 1233
BslI CCNNNNNNNGG 4 cut(s) 90, 730, 1210, 1211
BsmAI GTCTC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 3 cut(s) 319, 390, 1147
Bsp13I TCCGGA 2 cut(s) 1020, 1232
Bsp143I GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
BspACI CCGC 1 cut(s) 789
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 1 cut(s) 468
BspEI TCCGGA 2 cut(s) 1020, 1232
BspLI GGNNCC 2 cut(s) 318, 1019
BspMAI CTGCAG 1 cut(s) 602
BspPI GGATC 2 cut(s) 191, 1145
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 468
BsrI ACTGG 3 cut(s) 230, 320, 668
BssECI CCNNGG 6 cut(s) 311, 312, 333, 703, 921, 1110
BssMI GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
BssT1I CCWWGG 4 cut(s) 333, 703, 921, 1110
Bst2UI CCWGG 2 cut(s) 313, 328
Bst4CI ACNGT 1 cut(s) 1249
BstAPI GCANNNNNTGC 1 cut(s) 385
BstBAI YACGTR 1 cut(s) 399
BstC8I GCNNGC 2 cut(s) 291, 915
BstDEI CTNAG 1 cut(s) 476
BstEII GGTNACC 1 cut(s) 1169
BstF5I GGATG 2 cut(s) 20, 1106
BstKTI GATC 7 cut(s) 78, 178, 199, 427, 801, 1032, 1140
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
BstMWI GCNNNNNNNGC 2 cut(s) 385, 910
BstNI CCWGG 2 cut(s) 313, 328
BstNSI RCATGY 1 cut(s) 583
BstPI GGTNACC 1 cut(s) 1169
BstSCI CCNGG 3 cut(s) 311, 326, 1210
BstSFI CTRYAG 2 cut(s) 346, 598
BstV1I GCAGC 4 cut(s) 388, 440, 584, 904
BstV2I GAAGAC 2 cut(s) 354, 1031
BstX2I RGATCY 1 cut(s) 798
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 798
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 2 cut(s) 20, 1106
BtsIMutI CAGTG 2 cut(s) 473, 661
Cac8I GCNNGC 2 cut(s) 291, 915
Cfr13I GGNCC 2 cut(s) 192, 330
Csp6I GTAC 2 cut(s) 400, 838
CviAII CATG 6 cut(s) 43, 238, 262, 580, 718, 1127
CviQI GTAC 2 cut(s) 400, 838
DdeI CTNAG 1 cut(s) 476
DpnI GATC 7 cut(s) 77, 177, 198, 426, 800, 1031, 1139
DpnII GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
DraIII CACNNNGTG 1 cut(s) 385
Eco130I CCWWGG 4 cut(s) 333, 703, 921, 1110
Eco24I GRGCYC 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 560
Eco32I GATATC 2 cut(s) 259, 1089
Eco47I GGWCC 2 cut(s) 192, 330
Eco57I CTGAAG 2 cut(s) 320, 894
Eco91I GGTNACC 1 cut(s) 1169
EcoO65I GGTNACC 1 cut(s) 1169
EcoRII CCWGG 2 cut(s) 311, 326
EcoRV GATATC 2 cut(s) 259, 1089
EcoT14I CCWWGG 4 cut(s) 333, 703, 921, 1110
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 4 cut(s) 333, 703, 921, 1110
FaeI CATG 6 cut(s) 46, 241, 265, 583, 721, 1130
FatI CATG 6 cut(s) 42, 237, 261, 579, 717, 1126
FbaI TGATCA 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 377, 454, 598, 789, 918
FokI GGATG 2 cut(s) 7, 1093
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 5 cut(s) 377, 454, 598, 789, 918
FspBI CTAG 2 cut(s) 339, 503
GluI GCNGC 5 cut(s) 377, 454, 598, 789, 918
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 2 cut(s) 303, 1138
HaeIII GGCC 1 cut(s) 83
HapII CCGG 4 cut(s) 284, 1021, 1211, 1233
Hin1II CATG 6 cut(s) 46, 241, 265, 583, 721, 1130
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 5 cut(s) 97, 272, 407, 480, 699
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 4 cut(s) 284, 1021, 1211, 1233
HphI GGTGA 3 cut(s) 477, 1126, 1181
Hpy166II GTNNAC 2 cut(s) 402, 520
Hpy188I TCNGA 4 cut(s) 612, 828, 1137, 1142
Hpy188III TCNNGA 6 cut(s) 411, 746, 895, 1021, 1155, 1233
Hpy8I GTNNAC 2 cut(s) 402, 520
Hpy99I CGWCG 1 cut(s) 1008
HpyAV CCTTC 1 cut(s) 1007
HpyCH4III ACNGT 1 cut(s) 1249
HpyCH4IV ACGT 2 cut(s) 12, 398
HpyCH4V TGCA 8 cut(s) 56, 293, 461, 600, 886, 904, 1126, 1218
HpyF10VI GCNNNNNNNGC 2 cut(s) 385, 910
HpyF3I CTNAG 1 cut(s) 476
HpySE526I ACGT 2 cut(s) 12, 398
Hsp92II CATG 6 cut(s) 46, 241, 265, 583, 721, 1130
Kpn2I TCCGGA 2 cut(s) 1020, 1232
Ksp22I TGATCA 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
LmnI GCTCC 3 cut(s) 286, 322, 1142
Lsp1109I GCAGC 4 cut(s) 388, 440, 584, 904
LweI GCATC 3 cut(s) 515, 1009, 1227
MaeI CTAG 2 cut(s) 339, 503
MaeII ACGT 2 cut(s) 12, 398
MaeIII GTNAC 5 cut(s) 273, 341, 602, 1054, 1169
MalI GATC 7 cut(s) 77, 177, 198, 426, 800, 1031, 1139
MboI GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
MboII GAAGA 7 cut(s) 241, 354, 635, 808, 844, 1036, 1129
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 3 cut(s) 319, 390, 1147
MluCI AATT 8 cut(s) 232, 392, 753, 844, 862, 1049, 1100, 1228
MlyI GAGTC 2 cut(s) 281, 693
MmeI TCCRAC 1 cut(s) 1053
MnlI CCTC 8 cut(s) 315, 344, 463, 724, 834, 1160, 1177, 1229
MroI TCCGGA 2 cut(s) 1020, 1232
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 7 cut(s) 27, 210, 248, 519, 527, 563, 681
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 4 cut(s) 284, 1021, 1211, 1233
MspR9I CCNGG 3 cut(s) 313, 328, 1212
MunI CAATTG 1 cut(s) 753
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 2 cut(s) 313, 328
MwoI GCNNNNNNNGC 2 cut(s) 385, 910
NciI CCSGG 1 cut(s) 1212
NdeII GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
NlaIII CATG 6 cut(s) 46, 241, 265, 583, 721, 1130
NlaIV GGNNCC 2 cut(s) 318, 1019
NmuCI GTSAC 4 cut(s) 273, 602, 1054, 1169
NspI RCATGY 1 cut(s) 583
PasI CCCWGGG 1 cut(s) 312
PctI GAATGC 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 97, 407, 480
PkrI GCNGC 5 cut(s) 378, 455, 599, 790, 919
PleI GAGTC 2 cut(s) 280, 693
PpsI GAGTC 2 cut(s) 280, 693
Ppu21I YACGTR 1 cut(s) 399
PshBI ATTAAT 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 311, 326
PspEI GGTNACC 1 cut(s) 1169
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 2 cut(s) 311, 326
PspN4I GGNNCC 2 cut(s) 318, 1019
PspPI GGNCC 2 cut(s) 192, 330
PstI CTGCAG 1 cut(s) 602
PsuI RGATCY 1 cut(s) 798
RsaI GTAC 2 cut(s) 401, 839
RsaNI GTAC 2 cut(s) 400, 838
SaqAI TTAA 7 cut(s) 27, 210, 248, 519, 527, 563, 681
SatI GCNGC 5 cut(s) 377, 454, 598, 789, 918
Sau3AI GATC 7 cut(s) 75, 175, 196, 424, 798, 1029, 1137
Sau96I GGNCC 2 cut(s) 192, 330
SchI GAGTC 2 cut(s) 281, 693
ScrFI CCNGG 3 cut(s) 313, 328, 1212
SduI GDGCHC 3 cut(s) 319, 390, 1147
SfaNI GCATC 3 cut(s) 515, 1009, 1227
SfcI CTRYAG 2 cut(s) 346, 598
SinI GGWCC 2 cut(s) 192, 330
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
Sse9I AATT 8 cut(s) 232, 392, 753, 844, 862, 1049, 1100, 1228
SsiI CCGC 1 cut(s) 789
SspMI CTAG 2 cut(s) 339, 503
StyD4I CCNGG 3 cut(s) 311, 326, 1210
StyI CCWWGG 4 cut(s) 333, 703, 921, 1110
TaaI ACNGT 1 cut(s) 1249
TaiI ACGT 2 cut(s) 15, 401
TaqI TCGA 2 cut(s) 495, 1003
TaqII GACCGA 1 cut(s) 209
TasI AATT 8 cut(s) 232, 392, 753, 844, 862, 1049, 1100, 1228
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 3 cut(s) 97, 407, 480
Tru1I TTAA 7 cut(s) 27, 210, 248, 519, 527, 563, 681
Tru9I TTAA 7 cut(s) 27, 210, 248, 519, 527, 563, 681
TscAI CASTG 2 cut(s) 480, 668
TseFI GTSAC 4 cut(s) 273, 602, 1054, 1169
TseI GCWGC 4 cut(s) 376, 453, 597, 917
Tsp45I GTSAC 4 cut(s) 273, 602, 1054, 1169
TspDTI ATGAA 9 cut(s) 5, 59, 119, 161, 226, 426, 585, 946, 1143
TspGWI ACGGA 1 cut(s) 1095
TspRI CASTG 2 cut(s) 480, 668
VpaK11BI GGWCC 2 cut(s) 192, 330
VspI ATTAAT 1 cut(s) 681
XapI RAATTY 5 cut(s) 232, 392, 862, 1100, 1228
XceI RCATGY 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 2 cut(s) 339, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.