RchiOBHm_Chr2g0085811

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008517
Physical Location & Seq
Forward (+)
1087877 .. 1092157
4281 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46134

Sequence Viewer

Length: 2130 bp
ATGGATGTGGGTAGGAGGAGATCAGATGCAGGAGTGCTCAGAACTGGGAACAGTGTGGTTGCAGGGTCAGTGTGGGAGAACAGGATGAAGCTTGATGAAGTCAGAGGTGGGATTAAGGTCTTCAATGGTGATCAGGAGAATTCTGAAGAGAGCAGCAGCAGCAGTAATGGGGTTGCTGCAACAACAACTCCTAGCACAAGGTTGAAGAGAGGTCAATCTGTTGGTGGAGCAGTGAGTAATGGAAAGAGAAAGACTTGGAAATCTGATACAAGGGTTGAAGGTTTGGAGAAGAGTGGGGCTCAATTGTCCAGAGGAAAATCTGAGTTGAAGAATTATGATGAACAGTGCAAGGAGTTGAGTGCTTCTGTCGATGGGATCAAGAACAGCCCAACTCAGGTGAGGTCTGTGAGGAGACTTAGATCTGAGGGAAGCAAGGAGAGTCAAAATGCTGTTGCTTCTGATAAAGCTGAGAGGAGCCCAGTTGGCAGTAGGAAGCCAAGGACTGAATTGCTGAAAAGTGCTAGTGATTTGGGTAAGGAGGAGGGAATTGAGAAGAACTTGGGTCAGTTGAGGAAGGTGAAATCAGAATCGATCAGGGGAAGTGATTCTTCTGGCAATGCAATTCAATTGGGGAAGGTGAAATCGGAATTGAATAAGGCCTCCCCAGATGAATTGGATAAGGGAGTTGAGAAGTCTGTTGAGGTGATTGAGAAAATCCCAGTTGAGGAGGAGACTGGAGATGGTGGATCTGATGAGAATTGCAAGGAGTTTGGTGTGTGCAGAGAAATGGTGGTTCCAAGCAATGTGGATGTGGTCAAATCTGATCCAACAGAATTGGTGAATGATGTTGCTGATGATGAAGATGGTGATGAAGAAGAAGAATTGGAGGAAGAGGTGGATGAAGAAATTGAGGTTGAAGTTGAAAAGATGAGCCTTGATATTAAGGAAATCAATGCACCGGTACAAGAGAAAGCACCAGTTGAAGAAGAAGCGCCAGTTGAAGAAAAAGCACCAGTTCAAGTGAGAGCACCAGTTCATGAAAAAGCACCAGTTCAAGTGAGAGCACCAGTTCAAGAGAAAGTGAGAGCACCAGTGCAAGAGAAATTAAGCAAGGTTGTGAATGAAGTGAAGGTAGTGAATGAAGTTAAGAAGTTTCAATTATTTGACCGCAAATTTAGAAACCAAGAAGTGAAAAAGCTTCAACATGTTCATCAAAAGCCAGAAACGATCTCCGTTAATCTGAAAAAGCAATCTCCTGTTATGAAACGTGCAGAAGTCTACTCAAATGTCCCTAAACAAACATCATATGCAGCTCCAAATGATCAGTACCATAGCTTCCCAGAAACACACAGCAGATTGCAAAGTTTAGCGGATTTAGTAATGTGGAAAGATGTATCAAGATCAGTTTTTATATTTGGGATTGGAACATTTGTGATACTATCATCCTCCTACATGAAGGATCTAAATATCAGCTTCATTTCTGTCATATCCTATATGAGCCTTGTCTATCTGGCTGTCATCTTCCTCTTCAGGTCCATCATCTTGAGGGGAGCTTATGAAGTAGAAAATGCAGACTCTGTGGTGGGTGAAGAAGAAGCAATTTGGTTACTGAAACTGGTCCTGCCTTACTTGAATGAGTTCCTGCTAAAGTTTAAAGCTTTGTTTTCTGGTGACCCTGCCACCACAATTAAGTTGGCAGTGCTGCTCTTTGTTTTGGCCAGGTGTGGCAGCTCCATAACCATTTGGACAATGGCCAAAGTAGGCTTTTTCGGAGTTTTCACCGTACCAAAGGTGTGCTCTTTGTATTCTACCCAGATAACAGTCTTCGCTAAATTTTGGGTTCGGCGGTTTCGGGATGCTTGGGACTCCTGCTCTCACAAGAAACCAGTGACCATTGTGATCTTTCTACTAGTATGGAACTTATCTTCAGTAGAGGCTCGAATTTGGGCAGCATTCATTTTGGTGGTGGCGTTCCGATACTATCAACAGAAGTTGGTGACGGAGGAGTGGGTGGAAGATGATGTAGCAGCAGTAGAAGAAGAAGAAGAAGGAGCTGAAGAAAATTGGCAGGGAGCACAAAGAGCCACTCCAGTTGATATTCACATCACTAAACAAAAGAAAGGATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

709

Amino Acids

79.12

Weight (kDa)

5.54

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 457 - 610 1e-21 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1278
AciI CCGC 3 cut(s) 1168, 1370, 1846
AclWI GGATC 4 cut(s) 383, 754, 818, 1467
AcoI YGGCCR 2 cut(s) 1716, 1752
AcsI RAATTY 4 cut(s) 139, 1172, 1832, 1941
AcuI CTGAAG 4 cut(s) 165, 1513, 1911, 2076
AfaI GTAC 3 cut(s) 963, 1328, 1785
AfiI CCNNNNNNNGG 2 cut(s) 394, 724
AflIII ACRYGT 1 cut(s) 1204
AgeI ACCGGT 1 cut(s) 958
AhlI ACTAGT 1 cut(s) 1909
AjnI CCWGG 1 cut(s) 1718
Alw21I GWGCWC 6 cut(s) 39, 1030, 1066, 1090, 1799, 2077
Alw26I GTCTC 2 cut(s) 406, 725
AlwI GGATC 4 cut(s) 383, 754, 818, 1467
AlwNI CAGNNNCTG 1 cut(s) 1577
AoxI GGCC 3 cut(s) 657, 1716, 1752
ApeKI GCWGC 9 cut(s) 153, 156, 159, 176, 1310, 1702, 1728, 1949, 2027
ApoI RAATTY 4 cut(s) 139, 1172, 1832, 1941
AsiGI ACCGGT 1 cut(s) 958
Asp700I GAANNNNTTC 3 cut(s) 604, 1637, 2123
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 2 cut(s) 1533, 1618
AvaII GGWCC 2 cut(s) 1533, 1618
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BalI TGGCCA 2 cut(s) 1718, 1754
BanII GRGCYC 2 cut(s) 301, 479
BarI GAAGNNNNNNTAC 2 cut(s) 1319, 1351
BbsI GAAGAC 2 cut(s) 112, 1816
Bbv12I GWGCWC 6 cut(s) 39, 1030, 1066, 1090, 1799, 2077
BbvI GCAGC 9 cut(s) 163, 165, 168, 171, 1322, 1689, 1740, 1961, 2039
BccI CCATC 4 cut(s) 365, 734, 857, 1544
BciT130I CCWGG 1 cut(s) 1720
BclI TGATCA 2 cut(s) 130, 1321
BcoDI GTCTC 2 cut(s) 406, 725
BcuI ACTAGT 1 cut(s) 1909
BfaI CTAG 3 cut(s) 192, 522, 1910
BfoI RGCGCY 1 cut(s) 995
BglI GCCNNNNNGGC 1 cut(s) 483
BglII AGATCT 1 cut(s) 419
BisI GCNGC 9 cut(s) 154, 157, 160, 177, 1311, 1703, 1729, 1950, 2028
BlsI GCNGC 9 cut(s) 155, 158, 161, 178, 1312, 1704, 1730, 1951, 2029
Bme1390I CCNGG 1 cut(s) 1720
Bme18I GGWCC 2 cut(s) 1533, 1618
BmgT120I GGNCC 2 cut(s) 1533, 1618
BmiI GGNNCC 2 cut(s) 476, 795
BmrFI CCNGG 1 cut(s) 1720
BmrI ACTGGG 3 cut(s) 54, 473, 713
BmsI GCATC 2 cut(s) 16, 1846
BmuI ACTGGG 3 cut(s) 54, 473, 713
BpiI GAAGAC 2 cut(s) 112, 1816
BplI GAGNNNNNCTC 2 cut(s) 283, 315
BpmI CTGGAG 2 cut(s) 756, 2073
BpuEI CTTGAG 1 cut(s) 1564
Bsa29I ATCGAT 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 497
BsaWI WCCGGW 1 cut(s) 958
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 724
Bse118I RCCGGY 1 cut(s) 958
Bse3DI GCAATG 2 cut(s) 622, 808
BseBI CCWGG 1 cut(s) 1720
BseCI ATCGAT 1 cut(s) 590
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 6 cut(s) 10, 90, 814, 904, 1442, 1861
BseLI CCNNNNNNNGG 2 cut(s) 394, 724
BseMI GCAATG 2 cut(s) 622, 808
BseMII CTCAG 5 cut(s) 52, 312, 407, 414, 459
BseRI GAGGAG 7 cut(s) 31, 424, 487, 554, 740, 743, 2018
BseXI GCAGC 9 cut(s) 163, 165, 168, 171, 1322, 1689, 1740, 1961, 2039
BsgI GTGCAG 2 cut(s) 799, 1290
BshFI GGCC 3 cut(s) 659, 1718, 1754
BshTI ACCGGT 1 cut(s) 958
BshVI ATCGAT 1 cut(s) 590
BsiHKAI GWGCWC 6 cut(s) 39, 1030, 1066, 1090, 1799, 2077
BsiSI CCGG 1 cut(s) 959
BslFI GGGAC 2 cut(s) 1274, 1877
BslI CCNNNNNNNGG 2 cut(s) 394, 724
BsmAI GTCTC 2 cut(s) 406, 725
BsmFI GGGAC 2 cut(s) 1274, 1877
BsmI GAATGC 1 cut(s) 1952
BsnI GGCC 3 cut(s) 659, 1718, 1754
Bsp1286I GDGCHC 8 cut(s) 39, 301, 479, 1030, 1066, 1090, 1799, 2077
BspACI CCGC 3 cut(s) 1168, 1370, 1846
BspANI GGCC 3 cut(s) 659, 1718, 1754
BspCNI CTCAG 5 cut(s) 51, 313, 406, 415, 460
BspDI ATCGAT 1 cut(s) 590
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 2 cut(s) 476, 795
BspPI GGATC 4 cut(s) 383, 754, 818, 1467
BsrDI GCAATG 2 cut(s) 622, 808
BsrFI RCCGGY 1 cut(s) 958
BssAI RCCGGY 1 cut(s) 958
BssECI CCNNGG 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 1720
Bst4CI ACNGT 4 cut(s) 53, 345, 1783, 1822
Bst6I CTCTTC 5 cut(s) 141, 200, 284, 885, 1532
BstDEI CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
BstEII GGTNACC 1 cut(s) 1670
BstF5I GGATG 6 cut(s) 10, 90, 814, 904, 1442, 1861
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 1 cut(s) 994
BstMAI GTCTC 2 cut(s) 406, 725
BstMWI GCNNNNNNNGC 3 cut(s) 159, 483, 2081
BstNI CCWGG 1 cut(s) 1720
BstNSI RCATGY 1 cut(s) 1208
BstPI GGTNACC 1 cut(s) 1670
BstSCI CCNGG 1 cut(s) 1718
BstV1I GCAGC 9 cut(s) 163, 165, 168, 171, 1322, 1689, 1740, 1961, 2039
BstV2I GAAGAC 2 cut(s) 112, 1816
BstX2I RGATCY 3 cut(s) 419, 746, 1459
BstYI RGATCY 3 cut(s) 419, 746, 1459
Bsu15I ATCGAT 1 cut(s) 590
BsuRI GGCC 3 cut(s) 659, 1718, 1754
BsuTUI ATCGAT 1 cut(s) 590
BtsCI GGATG 6 cut(s) 10, 90, 814, 904, 1442, 1861
BtsI GCAGTG 2 cut(s) 237, 1704
BtsIMutI CAGTG 7 cut(s) 58, 75, 237, 350, 1098, 1704, 1893
CaiI CAGNNNCTG 1 cut(s) 1577
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 1 cut(s) 994
Cfr10I RCCGGY 1 cut(s) 958
Cfr13I GGNCC 2 cut(s) 1533, 1618
ClaI ATCGAT 1 cut(s) 590
Csp6I GTAC 3 cut(s) 962, 1327, 1784
CspAI ACCGGT 1 cut(s) 958
CspCI CAANNNNNGTGG 4 cut(s) 786, 821, 2074, 2109
CviAII CATG 3 cut(s) 1037, 1205, 1453
CviQI GTAC 3 cut(s) 962, 1327, 1784
DdeI CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
DraI TTTAAA 1 cut(s) 1654
EaeI YGGCCR 2 cut(s) 1716, 1752
Eam1104I CTCTTC 5 cut(s) 141, 200, 284, 885, 1532
EarI CTCTTC 5 cut(s) 141, 200, 284, 885, 1532
Eco130I CCWWGG 1 cut(s) 497
Eco147I AGGCCT 1 cut(s) 659
Eco24I GRGCYC 2 cut(s) 301, 479
Eco47I GGWCC 2 cut(s) 1533, 1618
Eco57I CTGAAG 4 cut(s) 165, 1513, 1911, 2076
Eco91I GGTNACC 1 cut(s) 1670
EcoO65I GGTNACC 1 cut(s) 1670
EcoRI GAATTC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 1718
EcoT14I CCWWGG 1 cut(s) 497
EcoT38I GRGCYC 2 cut(s) 301, 479
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 3 cut(s) 1040, 1208, 1456
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 2 cut(s) 1274, 1877
FatI CATG 3 cut(s) 1036, 1204, 1452
FauNDI CATATG 1 cut(s) 1306
FbaI TGATCA 2 cut(s) 130, 1321
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 9 cut(s) 154, 157, 160, 177, 1311, 1703, 1729, 1950, 2028
FokI GGATG 6 cut(s) 17, 97, 821, 911, 1429, 1868
FriOI GRGCYC 2 cut(s) 301, 479
Fsp4HI GCNGC 9 cut(s) 154, 157, 160, 177, 1311, 1703, 1729, 1950, 2028
FspBI CTAG 3 cut(s) 192, 522, 1910
GlaI GCGC 1 cut(s) 993
GluI GCNGC 9 cut(s) 154, 157, 160, 177, 1311, 1703, 1729, 1950, 2028
GsuI CTGGAG 2 cut(s) 756, 2073
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 3 cut(s) 659, 1718, 1754
HapII CCGG 1 cut(s) 959
HhaI GCGC 1 cut(s) 994
Hin1II CATG 3 cut(s) 1040, 1208, 1456
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HindIII AAGCTT 3 cut(s) 89, 1196, 1656
HinfI GANTC 6 cut(s) 439, 587, 605, 1574, 1865, 2124
HpaII CCGG 1 cut(s) 959
Hpy166II GTNNAC 1 cut(s) 1279
Hpy188III TCNNGA 8 cut(s) 134, 309, 379, 1037, 1073, 1398, 1543, 1853
Hpy8I GTNNAC 1 cut(s) 1279
HpyAV CCTTC 6 cut(s) 272, 568, 628, 1123, 1450, 2042
HpyCH4III ACNGT 4 cut(s) 53, 345, 1783, 1822
HpyCH4IV ACGT 1 cut(s) 1267
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 483, 2081
HpyF3I CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
HpySE526I ACGT 1 cut(s) 1267
Hsp92II CATG 3 cut(s) 1040, 1208, 1456
HspAI GCGC 1 cut(s) 992
Ksp22I TGATCA 2 cut(s) 130, 1321
LmnI GCTCC 7 cut(s) 227, 474, 1318, 1550, 1736, 2051, 2072
Lsp1109I GCAGC 9 cut(s) 163, 165, 168, 171, 1322, 1689, 1740, 1961, 2039
LweI GCATC 2 cut(s) 16, 1846
MaeI CTAG 3 cut(s) 192, 522, 1910
MaeII ACGT 1 cut(s) 1267
MaeIII GTNAC 4 cut(s) 1605, 1670, 1888, 1996
MfeI CAATTG 2 cut(s) 302, 626
MflI RGATCY 3 cut(s) 419, 746, 1459
MhlI GDGCHC 8 cut(s) 39, 301, 479, 1030, 1066, 1090, 1799, 2077
MlsI TGGCCA 2 cut(s) 1718, 1754
MluNI TGGCCA 2 cut(s) 1718, 1754
MlyI GAGTC 3 cut(s) 448, 1568, 1859
MmeI TCCRAC 1 cut(s) 851
Mox20I TGGCCA 2 cut(s) 1718, 1754
MroXI GAANNNNTTC 3 cut(s) 604, 1637, 2123
MscI TGGCCA 2 cut(s) 1718, 1754
MseI TTAA 7 cut(s) 114, 942, 1106, 1146, 1236, 1653, 1689
MslI CAYNNNNRTG 1 cut(s) 1961
Msp20I TGGCCA 2 cut(s) 1718, 1754
MspI CCGG 1 cut(s) 959
MspR9I CCNGG 1 cut(s) 1720
MunI CAATTG 2 cut(s) 302, 626
Mva1269I GAATGC 1 cut(s) 1952
MvaI CCWGG 1 cut(s) 1720
MwoI GCNNNNNNNGC 3 cut(s) 159, 483, 2081
NdeI CATATG 1 cut(s) 1306
NlaIII CATG 3 cut(s) 1040, 1208, 1456
NlaIV GGNNCC 2 cut(s) 476, 795
NmuCI GTSAC 3 cut(s) 1670, 1888, 1996
NspI RCATGY 1 cut(s) 1208
PagI TCATGA 1 cut(s) 1036
PceI AGGCCT 1 cut(s) 659
PciI ACATGT 1 cut(s) 1204
PctI GAATGC 1 cut(s) 1952
PdmI GAANNNNTTC 3 cut(s) 604, 1637, 2123
PfeI GAWTC 3 cut(s) 587, 605, 2124
PinAI ACCGGT 1 cut(s) 958
PkrI GCNGC 9 cut(s) 155, 158, 161, 178, 1312, 1704, 1730, 1951, 2029
PleI GAGTC 3 cut(s) 447, 1568, 1859
PpsI GAGTC 3 cut(s) 447, 1568, 1859
PscI ACATGT 1 cut(s) 1204
Psp6I CCWGG 1 cut(s) 1718
PspEI GGTNACC 1 cut(s) 1670
PspGI CCWGG 1 cut(s) 1718
PspN4I GGNNCC 2 cut(s) 476, 795
PspPI GGNCC 2 cut(s) 1533, 1618
PstNI CAGNNNCTG 1 cut(s) 1577
PsuI RGATCY 3 cut(s) 419, 746, 1459
RsaI GTAC 3 cut(s) 963, 1328, 1785
RsaNI GTAC 3 cut(s) 962, 1327, 1784
RseI CAYNNNNRTG 1 cut(s) 1961
SaqAI TTAA 7 cut(s) 114, 942, 1106, 1146, 1236, 1653, 1689
SatI GCNGC 9 cut(s) 154, 157, 160, 177, 1311, 1703, 1729, 1950, 2028
Sau96I GGNCC 2 cut(s) 1533, 1618
SchI GAGTC 3 cut(s) 448, 1568, 1859
ScrFI CCNGG 1 cut(s) 1720
SduI GDGCHC 8 cut(s) 39, 301, 479, 1030, 1066, 1090, 1799, 2077
SfaNI GCATC 2 cut(s) 16, 1846
SinI GGWCC 2 cut(s) 1533, 1618
SmiMI CAYNNNNRTG 1 cut(s) 1961
SmlI CTYRAG 1 cut(s) 1543
SmoI CTYRAG 1 cut(s) 1543
SpeI ACTAGT 1 cut(s) 1909
SseBI AGGCCT 1 cut(s) 659
SsiI CCGC 3 cut(s) 1168, 1370, 1846
SspMI CTAG 3 cut(s) 192, 522, 1910
StuI AGGCCT 1 cut(s) 659
StyD4I CCNGG 1 cut(s) 1718
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 4 cut(s) 53, 345, 1783, 1822
TaiI ACGT 1 cut(s) 1270
TaqI TCGA 3 cut(s) 369, 590, 1939
TfiI GAWTC 3 cut(s) 587, 605, 2124
Tru1I TTAA 7 cut(s) 114, 942, 1106, 1146, 1236, 1653, 1689
Tru9I TTAA 7 cut(s) 114, 942, 1106, 1146, 1236, 1653, 1689
TscAI CASTG 7 cut(s) 58, 75, 237, 350, 1098, 1704, 1893
TseFI GTSAC 3 cut(s) 1670, 1888, 1996
TseI GCWGC 9 cut(s) 153, 156, 159, 176, 1310, 1702, 1728, 1949, 2027
Tsp45I GTSAC 3 cut(s) 1670, 1888, 1996
TspGWI ACGGA 2 cut(s) 1222, 2015
TspRI CASTG 7 cut(s) 58, 75, 237, 350, 1098, 1704, 1893
VpaK11BI GGWCC 2 cut(s) 1533, 1618
XapI RAATTY 4 cut(s) 139, 1172, 1832, 1941
XceI RCATGY 1 cut(s) 1208
XcmI CCANNNNNNNNNTGG 2 cut(s) 1690, 1747
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 3 cut(s) 604, 1637, 2123
XspI CTAG 3 cut(s) 192, 522, 1910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.