Rh2BG013200

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008517
Physical Location & Seq
Forward (+)
986188 .. 990711
4524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG013200.1

Sequence Viewer

Length: 1929 bp
ATGGATGTGGGTAGGAGGAGATCAGATGCAGGAGTGCTCAGAACTGGGAACAGTGTGGTTGCAGGGTCAGTGTGGGAGAACAGGATGAAGCTTGATGAAGTCAGAGGTGGGATTAAGGTCTTCAATGGTGATCAGGAGAATTCTGAAGAGAGCAGCAGCAGCAGTAATGGGGTTGCTGCAACAACAACTCCTAGCACAAGGTTGAAGAGAGGTCAATCTGTTGGTGGAGCAGTGAGCAATGGGAAGAGAAAGACTTGGAAATCTGATACAAGGGTTGAAGGTTTGGAGAAGAGTGGGGCTCAATTGTCCAGAGGAAAATCTGAGTTGAAGAATTCTGATGAACAGTGCAAGGAGTTGAGTGTTTCTGTCGATGGGATCAAGAACAGCCCAACTCAGGTGAGGTCTGTGAGGAGACTTAGATCTGAGGGAAGCAAGGAGAGTCAAAATGCTGTTGCTTCTGATAAAGCTGAGAGGAGCCCAGTTGGGAGTAGGAAGCCAAGGACTGAATTGCTGAAAAGTGCTAGTGATTTGGGTAAGGAGGAGGGAATTGAGAAGAATTTGGGTCAGTTGAGGAAGGTGAAATCAGAGTCGATCAGGGGAAGTGATTCTTCTGGCAATGCAATTCAATTGGGGAAGGTGAAATCGGAATCGAATAAGGCCTCCCCAGATGAATTGGATAAGGGAGTTGACAAGTCTGTTGAGGTGATTGAGAAAATCCCAGTTGAGGAGGAGACTGGAGATGGTGGATCTGATGAGAATTGCAAGGAGTTTGGTGTGTGCAGAGAAATGGTGGTTCCAAGCAATGTGGATGTGGTCAAATCTGATCCAACAGAATTGGTGAATGATGTTGCTGATGATGAAGATGGTGATGAAGAAGGAGAAGAATTGGAGGAAGAGGTGGATGAAGAAATTGAGGTTGAAGTTGAAAAGATGAGCCTTGATATTAAGGAAATCAATGCACCGGTACAAGAGAATGCACCAGTTGAAGAAGAAGCACCAGTTGAAGAAAAAGCACCAGTTCAAGAAGAAGCACCAGTTCAAGTGAGAGCGCCAGTTCAAGAAAAAGCACCAGTTCAAGTGAGAGCACCAGTTCAAGAGAAAGTGAGAGCACCAGTGCAAGAGAAATTAAGCAAGGTTGTGAATGAAGTGAAGGTAGTGAATGAAGTTAAGAAGTTTCAATTATTTGGCCGCAAATTAAGAAACCAAGAAGTGAAAAAGCTTCAACATGTTCATCAAAAGCCAGAAACGATCTCCGTTAATCTGAAAAAGCAGTCTCCTGTTATGAAACGTGCAGAAGTCTACTCAAACGTCCCTAAACAAACTTCATATGCAGCTCCAAATGATCAGTACCATAGCTTCCCAGAAACACATAGCAGATTGCAAAGTTTAGCGGATTTAGTAATGTGGAAAGATGTATCAAGATCAGTTTTTATATTTGGGATTGGAACATTTGTGATACTATCATCCTCCTACATGAAGGATCTAAATATCAGCTTCATTTCTGTCATATCCTATATGAGCCTTGTTTATCTGGCTGTCATCTTCCTCTTCAGGTCCATCATCTTGAGGGGAGCTTATGAAATAGAAAATGCAAACTCTGTGGTGGGTGAAGAAGAAGCAATTTGGTTACTGAAACTGGTCCTGCCTTACTTGAATGAGTTCCTGCTAAAGTTTAAAGCTTTGTTTTCTGGTGACCCTGCCACCACAATTAAGTTGGCAGTGCTGCTCTTTGTTTTGGCCAGGTGTGGCAGCTCCATAACCATTTGGACAATGGCCAAAGTAGGCTTTTTCGGAGTTTTCACCGTACCAAAAGTGTGCTCTTTGTATTCTACCCAGATAACAATCTTCGGTAAATTCCTGCAATCATTTTCTCCCTTACTAATCATGCATAGCATAGTATTCATTGCCCAAAAAAAAAATCATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

642

Amino Acids

70.85

Weight (kDa)

5.79

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 464 - 614 1.6e-21 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1299
AciI CCGC 2 cut(s) 1189, 1391
AclWI GGATC 4 cut(s) 383, 754, 818, 1488
AcoI YGGCCR 3 cut(s) 1186, 1737, 1773
AcsI RAATTY 4 cut(s) 139, 331, 556, 1853
AcuI CTGAAG 2 cut(s) 165, 1534
AfaI GTAC 3 cut(s) 966, 1349, 1806
AfiI CCNNNNNNNGG 2 cut(s) 394, 724
AflIII ACRYGT 1 cut(s) 1225
AgeI ACCGGT 1 cut(s) 961
AjnI CCWGG 1 cut(s) 1739
AluBI AGCT 9 cut(s) 91, 467, 1219, 1334, 1356, 1494, 1574, 1679, 1752
AluI AGCT 9 cut(s) 91, 467, 1219, 1334, 1356, 1494, 1574, 1679, 1752
Alw21I GWGCWC 4 cut(s) 39, 1087, 1111, 1820
Alw26I GTCTC 3 cut(s) 406, 725, 1278
AlwI GGATC 4 cut(s) 383, 754, 818, 1488
AoxI GGCC 4 cut(s) 657, 1186, 1737, 1773
ApeKI GCWGC 7 cut(s) 153, 156, 159, 176, 1331, 1723, 1749
ApoI RAATTY 4 cut(s) 139, 331, 556, 1853
AsiGI ACCGGT 1 cut(s) 961
Asp700I GAANNNNTTC 2 cut(s) 604, 1658
AspLEI GCGC 1 cut(s) 1051
AspS9I GGNCC 2 cut(s) 1554, 1639
AvaII GGWCC 2 cut(s) 1554, 1639
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BalI TGGCCA 2 cut(s) 1739, 1775
BanII GRGCYC 2 cut(s) 301, 479
BarI GAAGNNNNNNTAC 2 cut(s) 1340, 1372
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 4 cut(s) 39, 1087, 1111, 1820
BbvI GCAGC 7 cut(s) 163, 165, 168, 171, 1343, 1710, 1761
BccI CCATC 4 cut(s) 365, 734, 857, 1565
BciT130I CCWGG 1 cut(s) 1741
BclI TGATCA 2 cut(s) 130, 1342
BcoDI GTCTC 3 cut(s) 406, 725, 1278
BfaI CTAG 2 cut(s) 192, 522
BfoI RGCGCY 1 cut(s) 1052
BglII AGATCT 1 cut(s) 419
BisI GCNGC 8 cut(s) 154, 157, 160, 177, 1189, 1332, 1724, 1750
BlsI GCNGC 8 cut(s) 155, 158, 161, 178, 1190, 1333, 1725, 1751
Bme1390I CCNGG 1 cut(s) 1741
Bme18I GGWCC 2 cut(s) 1554, 1639
BmgT120I GGNCC 2 cut(s) 1554, 1639
BmiI GGNNCC 2 cut(s) 476, 795
BmrFI CCNGG 1 cut(s) 1741
BmrI ACTGGG 3 cut(s) 54, 473, 713
BmsI GCATC 1 cut(s) 16
BmuI ACTGGG 3 cut(s) 54, 473, 713
BpiI GAAGAC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 283, 315
BpmI CTGGAG 1 cut(s) 756
BpuEI CTTGAG 1 cut(s) 1585
BsaBI GATNNNNATC 1 cut(s) 1841
BsaJI CCNNGG 1 cut(s) 497
BsaWI WCCGGW 1 cut(s) 961
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 724
Bse118I RCCGGY 1 cut(s) 961
Bse3DI GCAATG 4 cut(s) 244, 622, 808, 1902
Bse8I GATNNNNATC 1 cut(s) 1841
BseBI CCWGG 1 cut(s) 1741
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 5 cut(s) 10, 90, 814, 907, 1463
BseJI GATNNNNATC 1 cut(s) 1841
BseLI CCNNNNNNNGG 2 cut(s) 394, 724
BseMI GCAATG 4 cut(s) 244, 622, 808, 1902
BseMII CTCAG 5 cut(s) 52, 312, 407, 414, 459
BseRI GAGGAG 6 cut(s) 31, 424, 487, 554, 740, 743
BseXI GCAGC 7 cut(s) 163, 165, 168, 171, 1343, 1710, 1761
BsgI GTGCAG 2 cut(s) 799, 1311
BshFI GGCC 4 cut(s) 659, 1188, 1739, 1775
BshTI ACCGGT 1 cut(s) 961
BsiHKAI GWGCWC 4 cut(s) 39, 1087, 1111, 1820
BsiSI CCGG 1 cut(s) 962
BslFI GGGAC 1 cut(s) 1295
BslI CCNNNNNNNGG 2 cut(s) 394, 724
BsmAI GTCTC 3 cut(s) 406, 725, 1278
BsmFI GGGAC 1 cut(s) 1295
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 4 cut(s) 659, 1188, 1739, 1775
Bsp1286I GDGCHC 6 cut(s) 39, 301, 479, 1087, 1111, 1820
BspACI CCGC 2 cut(s) 1189, 1391
BspANI GGCC 4 cut(s) 659, 1188, 1739, 1775
BspCNI CTCAG 5 cut(s) 51, 313, 406, 415, 460
BspLI GGNNCC 2 cut(s) 476, 795
BspPI GGATC 4 cut(s) 383, 754, 818, 1488
BsrDI GCAATG 4 cut(s) 244, 622, 808, 1902
BsrFI RCCGGY 1 cut(s) 961
BssAI RCCGGY 1 cut(s) 961
BssECI CCNNGG 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 1741
Bst4CI ACNGT 3 cut(s) 53, 345, 1804
Bst6I CTCTTC 6 cut(s) 141, 200, 239, 284, 888, 1553
BstDEI CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
BstEII GGTNACC 1 cut(s) 1691
BstF5I GGATG 5 cut(s) 10, 90, 814, 907, 1463
BstH2I RGCGCY 1 cut(s) 1052
BstHHI GCGC 1 cut(s) 1051
BstMAI GTCTC 3 cut(s) 406, 725, 1278
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 1741
BstNSI RCATGY 1 cut(s) 1229
BstPI GGTNACC 1 cut(s) 1691
BstSCI CCNGG 1 cut(s) 1739
BstV1I GCAGC 7 cut(s) 163, 165, 168, 171, 1343, 1710, 1761
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 3 cut(s) 419, 746, 1480
BstYI RGATCY 3 cut(s) 419, 746, 1480
BsuRI GGCC 4 cut(s) 659, 1188, 1739, 1775
BtsCI GGATG 5 cut(s) 10, 90, 814, 907, 1463
BtsI GCAGTG 2 cut(s) 237, 1725
BtsIMutI CAGTG 6 cut(s) 58, 75, 237, 350, 1119, 1725
CfoI GCGC 1 cut(s) 1051
Cfr10I RCCGGY 1 cut(s) 961
Cfr13I GGNCC 2 cut(s) 1554, 1639
Csp6I GTAC 3 cut(s) 965, 1348, 1805
CspAI ACCGGT 1 cut(s) 961
CspCI CAANNNNNGTGG 4 cut(s) 786, 821, 1581, 1616
CviAII CATG 4 cut(s) 1226, 1474, 1885, 1922
CviQI GTAC 3 cut(s) 965, 1348, 1805
DdeI CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
DraI TTTAAA 1 cut(s) 1675
EaeI YGGCCR 3 cut(s) 1186, 1737, 1773
Eam1104I CTCTTC 6 cut(s) 141, 200, 239, 284, 888, 1553
EarI CTCTTC 6 cut(s) 141, 200, 239, 284, 888, 1553
Eco130I CCWWGG 1 cut(s) 497
Eco147I AGGCCT 1 cut(s) 659
Eco24I GRGCYC 2 cut(s) 301, 479
Eco47I GGWCC 2 cut(s) 1554, 1639
Eco57I CTGAAG 2 cut(s) 165, 1534
Eco91I GGTNACC 1 cut(s) 1691
EcoO65I GGTNACC 1 cut(s) 1691
EcoRI GAATTC 2 cut(s) 139, 331
EcoRII CCWGG 1 cut(s) 1739
EcoT14I CCWWGG 1 cut(s) 497
EcoT22I ATGCAT 2 cut(s) 1890, 1927
EcoT38I GRGCYC 2 cut(s) 301, 479
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 4 cut(s) 1229, 1477, 1888, 1925
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 1295
FatI CATG 4 cut(s) 1225, 1473, 1884, 1921
FauNDI CATATG 1 cut(s) 1327
FbaI TGATCA 2 cut(s) 130, 1342
FblI GTMKAC 1 cut(s) 1299
Fnu4HI GCNGC 8 cut(s) 154, 157, 160, 177, 1189, 1332, 1724, 1750
FokI GGATG 5 cut(s) 17, 97, 821, 914, 1450
FriOI GRGCYC 2 cut(s) 301, 479
Fsp4HI GCNGC 8 cut(s) 154, 157, 160, 177, 1189, 1332, 1724, 1750
FspBI CTAG 2 cut(s) 192, 522
GlaI GCGC 1 cut(s) 1050
GluI GCNGC 8 cut(s) 154, 157, 160, 177, 1189, 1332, 1724, 1750
GsuI CTGGAG 1 cut(s) 756
HaeII RGCGCY 1 cut(s) 1052
HaeIII GGCC 4 cut(s) 659, 1188, 1739, 1775
HapII CCGG 1 cut(s) 962
HhaI GCGC 1 cut(s) 1051
Hin1II CATG 4 cut(s) 1229, 1477, 1888, 1925
Hin6I GCGC 1 cut(s) 1049
HinP1I GCGC 1 cut(s) 1049
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 3 cut(s) 89, 1217, 1677
HinfI GANTC 4 cut(s) 439, 587, 605, 647
HpaII CCGG 1 cut(s) 962
Hpy166II GTNNAC 2 cut(s) 688, 1300
Hpy188III TCNNGA 8 cut(s) 134, 309, 379, 1022, 1058, 1094, 1419, 1564
Hpy8I GTNNAC 2 cut(s) 688, 1300
HpyAV CCTTC 6 cut(s) 272, 568, 628, 869, 1144, 1471
HpyCH4III ACNGT 3 cut(s) 53, 345, 1804
HpyCH4IV ACGT 2 cut(s) 1288, 1308
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
HpySE526I ACGT 2 cut(s) 1288, 1308
Hsp92II CATG 4 cut(s) 1229, 1477, 1888, 1925
HspAI GCGC 1 cut(s) 1049
Ksp22I TGATCA 2 cut(s) 130, 1342
LmnI GCTCC 5 cut(s) 227, 474, 1339, 1571, 1757
Lsp1109I GCAGC 7 cut(s) 163, 165, 168, 171, 1343, 1710, 1761
LweI GCATC 1 cut(s) 16
MaeI CTAG 2 cut(s) 192, 522
MaeII ACGT 2 cut(s) 1288, 1308
MaeIII GTNAC 2 cut(s) 1626, 1691
MfeI CAATTG 2 cut(s) 302, 626
MflI RGATCY 3 cut(s) 419, 746, 1480
MhlI GDGCHC 6 cut(s) 39, 301, 479, 1087, 1111, 1820
MlsI TGGCCA 2 cut(s) 1739, 1775
MluNI TGGCCA 2 cut(s) 1739, 1775
MlyI GAGTC 2 cut(s) 448, 596
MmeI TCCRAC 1 cut(s) 851
Mox20I TGGCCA 2 cut(s) 1739, 1775
Mph1103I ATGCAT 2 cut(s) 1890, 1927
MroXI GAANNNNTTC 2 cut(s) 604, 1658
MscI TGGCCA 2 cut(s) 1739, 1775
MseI TTAA 8 cut(s) 114, 945, 1127, 1167, 1196, 1257, 1674, 1710
Msp20I TGGCCA 2 cut(s) 1739, 1775
MspI CCGG 1 cut(s) 962
MspR9I CCNGG 1 cut(s) 1741
MunI CAATTG 2 cut(s) 302, 626
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 1 cut(s) 1741
MwoI GCNNNNNNNGC 1 cut(s) 159
NdeI CATATG 1 cut(s) 1327
NlaIII CATG 4 cut(s) 1229, 1477, 1888, 1925
NlaIV GGNNCC 2 cut(s) 476, 795
NmuCI GTSAC 1 cut(s) 1691
NsiI ATGCAT 2 cut(s) 1890, 1927
NspI RCATGY 1 cut(s) 1229
PceI AGGCCT 1 cut(s) 659
PciI ACATGT 1 cut(s) 1225
PctI GAATGC 1 cut(s) 979
PdmI GAANNNNTTC 2 cut(s) 604, 1658
PfeI GAWTC 2 cut(s) 605, 647
PinAI ACCGGT 1 cut(s) 961
PkrI GCNGC 8 cut(s) 155, 158, 161, 178, 1190, 1333, 1725, 1751
PleI GAGTC 2 cut(s) 447, 595
PpsI GAGTC 2 cut(s) 447, 595
PscI ACATGT 1 cut(s) 1225
Psp6I CCWGG 1 cut(s) 1739
PspEI GGTNACC 1 cut(s) 1691
PspGI CCWGG 1 cut(s) 1739
PspN4I GGNNCC 2 cut(s) 476, 795
PspPI GGNCC 2 cut(s) 1554, 1639
PsuI RGATCY 3 cut(s) 419, 746, 1480
RsaI GTAC 3 cut(s) 966, 1349, 1806
RsaNI GTAC 3 cut(s) 965, 1348, 1805
SaqAI TTAA 8 cut(s) 114, 945, 1127, 1167, 1196, 1257, 1674, 1710
SatI GCNGC 8 cut(s) 154, 157, 160, 177, 1189, 1332, 1724, 1750
Sau96I GGNCC 2 cut(s) 1554, 1639
SchI GAGTC 2 cut(s) 448, 596
ScrFI CCNGG 1 cut(s) 1741
SduI GDGCHC 6 cut(s) 39, 301, 479, 1087, 1111, 1820
SfaNI GCATC 1 cut(s) 16
SinI GGWCC 2 cut(s) 1554, 1639
SmlI CTYRAG 1 cut(s) 1564
SmoI CTYRAG 1 cut(s) 1564
SseBI AGGCCT 1 cut(s) 659
SsiI CCGC 2 cut(s) 1189, 1391
SspMI CTAG 2 cut(s) 192, 522
StuI AGGCCT 1 cut(s) 659
StyD4I CCNGG 1 cut(s) 1739
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 3 cut(s) 53, 345, 1804
TaiI ACGT 2 cut(s) 1291, 1311
TaqI TCGA 3 cut(s) 369, 590, 650
TauI GCSGC 1 cut(s) 1191
TfiI GAWTC 2 cut(s) 605, 647
Tru1I TTAA 8 cut(s) 114, 945, 1127, 1167, 1196, 1257, 1674, 1710
Tru9I TTAA 8 cut(s) 114, 945, 1127, 1167, 1196, 1257, 1674, 1710
TscAI CASTG 6 cut(s) 58, 75, 237, 350, 1119, 1725
TseFI GTSAC 1 cut(s) 1691
TseI GCWGC 7 cut(s) 153, 156, 159, 176, 1331, 1723, 1749
Tsp45I GTSAC 1 cut(s) 1691
TspGWI ACGGA 1 cut(s) 1243
TspRI CASTG 6 cut(s) 58, 75, 237, 350, 1119, 1725
VpaK11BI GGWCC 2 cut(s) 1554, 1639
XapI RAATTY 4 cut(s) 139, 331, 556, 1853
XceI RCATGY 1 cut(s) 1229
XcmI CCANNNNNNNNNTGG 2 cut(s) 1711, 1768
XmiI GTMKAC 1 cut(s) 1299
XmnI GAANNNNTTC 2 cut(s) 604, 1658
XspI CTAG 2 cut(s) 192, 522
Zsp2I ATGCAT 2 cut(s) 1890, 1927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.