Rh2DG014900

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008517
Physical Location & Seq
Forward (+)
837566 .. 841856
4291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG014900.1

Sequence Viewer

Length: 1776 bp
ATGGATGTGGGTAGAAGGAGATCAGATGCAGGAGTGCTCAGAACTGGGAACAGTGTGGTTGCAGGGTCAGTGTGGGAGAACAGGATGAAGCTTGATGAAGTCAGAGGTGGGATTAAGGTCTTCAATGGTGATCAGGAGAATTCTGAAGAGAGCAGCAGCAGCAGTAATGGGGTTGCTGCAACAACAACTCCTAGCACAAGGTTGAAGAGAGGTCAATCTGTTGGTGGAGCAGTGAGTAATGGAAAGAGAAAGACTTGGAAATCTGATACAAGGGTTGAAGGTTTGGAGAAGAGTGGGGCTCAATTGTCCAGAGGAAAATCTGAGTTGAAGAATTCTGATGAACAGTGCAAGGAGTTGAGTGTTTCTGTCGATGGGATCAAGAACAGCCCAACTCAGGTGAGGTCTGTGAGGAGACTTAGATCTGAGGGAAGCAAGGAGAGTCAAAATGCTGTTGCTTCTGATAAAGCTGAGAGGAGCCCAGTTGGCAGTAGGAAGGCAAGGACTGAATTGCTGAAAAGTGCTAGTGATTTGGGTAAGGAGGAGGGAATTGAGAAGAACTTGGGTCAGTTGAGGAAGGTGAAATCAGAGTCGATCAGGGGAAGTGATTCTTCTGCCAATGCAATTCAATTGGGGAAGGTGAAATCGGAATTGAATAAGGCCTCCCCAGATGAATTGGATAAGGGAGTTGAGAAGTCTGTTGAGGTGATTGAGAAAATCCCAGTTGAGGAGGAGACTGGAGATGGTGGATCTGATGAGAATTGCAAGGAGTTTGGTGTGTGCAGAGAAATGGTGGTTCCAAGCAATGTGGATGTGGTCAAATCTGATCCAACAGAATTGGTGAATGATGTTGCTGATGATGAAGATGGTGATGAAGAAGAAGAAGGAGAAGAATTGGAGGAAGAGGTGGATGAAGAAATTGAGGTTGAAGTTGAAAAGATGAGCCTTGATATTAAGGAAATCAATGCACCGGTACAAGAGAAAGCACCAGTTGAAGAAGAAGCGCCAGTTGAAGAAAAAGCACCAGTTCAAGTGAGAGCACCAGTTCAAGAAAAAGCACCAGTTCAAGTGAGAGCACCAGTTCAAGAGAAAGTGAGAGCACCAGTGCAAGAGAAATTAAGCAAGGTTGTGAATGAAGTGAAGGTAGTGAATGAAGTTAAGAAGTTTCAATTATTTGACCGCAAATTAAGAAACCAAGAAGTGAAAAAGCTTCAACATGTTCATCAAAAGCCAGAAACGATCTCCGTTAATCTGAAAAAGCAATCTCCTGTTATGAAACGTGCAGAAGTCTACTCAAATGTCCCTAAACAAACATCATATGCAGCTCCAAATGATCAGTACCATAGCTTCCCAGAAACACACAGCAGATTGCAAAGTTTAGCGGATTTAGTAATGTGGAAAGATGTATCAAGATCAGTTTTTATATTTGGGATTGGAACATTTGTGATACTATCATCCTCCTACATGAAGGATCTAAATATCAGCTTCATTTCTGTCATATCCTATATGAGCCTTGTTTATCTGGCTGTCATCTTCCTCTTCAGGTCCATCATCTTGAGGGGAGCTTATGAAATAGAAAATGCAAATTCTGTGGTGGGTGAAGAAGAAGCAATTTGGTTACTGAAACTGGTCCTGCCTTACTTGAATGAGTTCCTGCTAAAGTTTAAAGCTTTGTTTTCTGGTGACCCTGCCACCACAATTAAGTTGGCGGTGCTGCTCTTTGTTTTGGCCAGGTGTGGCAGCTCCATAACCATTTGGACAATGGCCAAAGTAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

591

Amino Acids

65.24

Weight (kDa)

5.41

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 460 - 588 6.8e-16 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1287
AciI CCGC 3 cut(s) 1177, 1379, 1706
AclWI GGATC 4 cut(s) 383, 754, 818, 1476
AcoI YGGCCR 2 cut(s) 1725, 1761
AcsI RAATTY 3 cut(s) 139, 331, 1582
AcuI CTGAAG 2 cut(s) 165, 1522
AfaI GTAC 2 cut(s) 972, 1337
AfiI CCNNNNNNNGG 2 cut(s) 394, 724
AflIII ACRYGT 1 cut(s) 1213
AgeI ACCGGT 1 cut(s) 967
AjnI CCWGG 1 cut(s) 1727
AluBI AGCT 9 cut(s) 91, 467, 1207, 1322, 1344, 1482, 1562, 1667, 1740
AluI AGCT 9 cut(s) 91, 467, 1207, 1322, 1344, 1482, 1562, 1667, 1740
Alw21I GWGCWC 4 cut(s) 39, 1039, 1075, 1099
Alw26I GTCTC 2 cut(s) 406, 725
AlwI GGATC 4 cut(s) 383, 754, 818, 1476
AoxI GGCC 3 cut(s) 657, 1725, 1761
ApeKI GCWGC 7 cut(s) 153, 156, 159, 176, 1319, 1711, 1737
ApoI RAATTY 3 cut(s) 139, 331, 1582
AsiGI ACCGGT 1 cut(s) 967
Asp700I GAANNNNTTC 2 cut(s) 604, 1646
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 2 cut(s) 1542, 1627
AsuHPI GGTGA 9 cut(s) 140, 409, 589, 649, 715, 850, 878, 1607, 1691
AvaII GGWCC 2 cut(s) 1542, 1627
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BalI TGGCCA 2 cut(s) 1727, 1763
BanII GRGCYC 2 cut(s) 301, 479
BarI GAAGNNNNNNTAC 2 cut(s) 1328, 1360
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 4 cut(s) 39, 1039, 1075, 1099
BbvI GCAGC 7 cut(s) 163, 165, 168, 171, 1331, 1698, 1749
BccI CCATC 4 cut(s) 365, 734, 857, 1553
BciT130I CCWGG 1 cut(s) 1729
BclI TGATCA 2 cut(s) 130, 1330
BcoDI GTCTC 2 cut(s) 406, 725
BfaI CTAG 2 cut(s) 192, 522
BfoI RGCGCY 1 cut(s) 1004
BglI GCCNNNNNGGC 1 cut(s) 483
BglII AGATCT 1 cut(s) 419
BisI GCNGC 7 cut(s) 154, 157, 160, 177, 1320, 1712, 1738
BlsI GCNGC 7 cut(s) 155, 158, 161, 178, 1321, 1713, 1739
Bme1390I CCNGG 1 cut(s) 1729
Bme18I GGWCC 2 cut(s) 1542, 1627
BmgT120I GGNCC 2 cut(s) 1542, 1627
BmiI GGNNCC 2 cut(s) 476, 795
BmrFI CCNGG 1 cut(s) 1729
BmrI ACTGGG 3 cut(s) 54, 473, 713
BmsI GCATC 1 cut(s) 16
BmuI ACTGGG 3 cut(s) 54, 473, 713
BpiI GAAGAC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 283, 315
BpmI CTGGAG 1 cut(s) 756
BpuEI CTTGAG 1 cut(s) 1573
BsaWI WCCGGW 1 cut(s) 967
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 724
Bse118I RCCGGY 1 cut(s) 967
Bse3DI GCAATG 1 cut(s) 808
BseBI CCWGG 1 cut(s) 1729
BseGI GGATG 5 cut(s) 10, 90, 814, 913, 1451
BseLI CCNNNNNNNGG 2 cut(s) 394, 724
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 5 cut(s) 52, 312, 407, 414, 459
BseRI GAGGAG 5 cut(s) 424, 487, 554, 740, 743
BseXI GCAGC 7 cut(s) 163, 165, 168, 171, 1331, 1698, 1749
BsgI GTGCAG 2 cut(s) 799, 1299
BshFI GGCC 3 cut(s) 659, 1727, 1763
BshTI ACCGGT 1 cut(s) 967
BsiHKAI GWGCWC 4 cut(s) 39, 1039, 1075, 1099
BsiSI CCGG 1 cut(s) 968
BslFI GGGAC 1 cut(s) 1283
BslI CCNNNNNNNGG 2 cut(s) 394, 724
BsmAI GTCTC 2 cut(s) 406, 725
BsmFI GGGAC 1 cut(s) 1283
BsnI GGCC 3 cut(s) 659, 1727, 1763
Bsp1286I GDGCHC 6 cut(s) 39, 301, 479, 1039, 1075, 1099
BspACI CCGC 3 cut(s) 1177, 1379, 1706
BspANI GGCC 3 cut(s) 659, 1727, 1763
BspCNI CTCAG 5 cut(s) 51, 313, 406, 415, 460
BspLI GGNNCC 2 cut(s) 476, 795
BspPI GGATC 4 cut(s) 383, 754, 818, 1476
BsrDI GCAATG 1 cut(s) 808
BsrFI RCCGGY 1 cut(s) 967
BssAI RCCGGY 1 cut(s) 967
Bst2UI CCWGG 1 cut(s) 1729
Bst4CI ACNGT 2 cut(s) 53, 345
Bst6I CTCTTC 5 cut(s) 141, 200, 284, 894, 1541
BstDEI CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
BstEII GGTNACC 1 cut(s) 1679
BstF5I GGATG 5 cut(s) 10, 90, 814, 913, 1451
BstH2I RGCGCY 1 cut(s) 1004
BstHHI GCGC 1 cut(s) 1003
BstMAI GTCTC 2 cut(s) 406, 725
BstMWI GCNNNNNNNGC 2 cut(s) 159, 483
BstNI CCWGG 1 cut(s) 1729
BstNSI RCATGY 1 cut(s) 1217
BstPI GGTNACC 1 cut(s) 1679
BstSCI CCNGG 1 cut(s) 1727
BstV1I GCAGC 7 cut(s) 163, 165, 168, 171, 1331, 1698, 1749
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 3 cut(s) 419, 746, 1468
BstYI RGATCY 3 cut(s) 419, 746, 1468
BsuRI GGCC 3 cut(s) 659, 1727, 1763
BtsCI GGATG 5 cut(s) 10, 90, 814, 913, 1451
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 5 cut(s) 58, 75, 237, 350, 1107
CfoI GCGC 1 cut(s) 1003
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 2 cut(s) 1542, 1627
Csp6I GTAC 2 cut(s) 971, 1336
CspAI ACCGGT 1 cut(s) 967
CspCI CAANNNNNGTGG 4 cut(s) 786, 821, 1569, 1604
CviAII CATG 2 cut(s) 1214, 1462
CviQI GTAC 2 cut(s) 971, 1336
DdeI CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
DraI TTTAAA 1 cut(s) 1663
EaeI YGGCCR 2 cut(s) 1725, 1761
Eam1104I CTCTTC 5 cut(s) 141, 200, 284, 894, 1541
EarI CTCTTC 5 cut(s) 141, 200, 284, 894, 1541
Eco147I AGGCCT 1 cut(s) 659
Eco24I GRGCYC 2 cut(s) 301, 479
Eco47I GGWCC 2 cut(s) 1542, 1627
Eco57I CTGAAG 2 cut(s) 165, 1522
Eco91I GGTNACC 1 cut(s) 1679
EcoO65I GGTNACC 1 cut(s) 1679
EcoRI GAATTC 2 cut(s) 139, 331
EcoRII CCWGG 1 cut(s) 1727
EcoT38I GRGCYC 2 cut(s) 301, 479
FaeI CATG 2 cut(s) 1217, 1465
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 1283
FatI CATG 2 cut(s) 1213, 1461
FauNDI CATATG 1 cut(s) 1315
FbaI TGATCA 2 cut(s) 130, 1330
FblI GTMKAC 1 cut(s) 1287
Fnu4HI GCNGC 7 cut(s) 154, 157, 160, 177, 1320, 1712, 1738
FokI GGATG 5 cut(s) 17, 97, 821, 920, 1438
FriOI GRGCYC 2 cut(s) 301, 479
Fsp4HI GCNGC 7 cut(s) 154, 157, 160, 177, 1320, 1712, 1738
FspBI CTAG 2 cut(s) 192, 522
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 7 cut(s) 154, 157, 160, 177, 1320, 1712, 1738
GsuI CTGGAG 1 cut(s) 756
HaeII RGCGCY 1 cut(s) 1004
HaeIII GGCC 3 cut(s) 659, 1727, 1763
HapII CCGG 1 cut(s) 968
HhaI GCGC 1 cut(s) 1003
Hin1II CATG 2 cut(s) 1217, 1465
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HindIII AAGCTT 3 cut(s) 89, 1205, 1665
HinfI GANTC 3 cut(s) 439, 587, 605
HpaII CCGG 1 cut(s) 968
HphI GGTGA 9 cut(s) 140, 409, 589, 649, 715, 850, 878, 1607, 1691
Hpy166II GTNNAC 1 cut(s) 1288
Hpy188III TCNNGA 7 cut(s) 134, 309, 379, 1046, 1082, 1407, 1552
Hpy8I GTNNAC 1 cut(s) 1288
HpyAV CCTTC 8 cut(s) 9, 272, 487, 568, 628, 875, 1132, 1459
HpyCH4III ACNGT 2 cut(s) 53, 345
HpyCH4IV ACGT 1 cut(s) 1276
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 483
HpyF3I CTNAG 6 cut(s) 38, 321, 393, 416, 423, 468
HpySE526I ACGT 1 cut(s) 1276
Hsp92II CATG 2 cut(s) 1217, 1465
HspAI GCGC 1 cut(s) 1001
Ksp22I TGATCA 2 cut(s) 130, 1330
LmnI GCTCC 5 cut(s) 227, 474, 1327, 1559, 1745
Lsp1109I GCAGC 7 cut(s) 163, 165, 168, 171, 1331, 1698, 1749
LweI GCATC 1 cut(s) 16
MaeI CTAG 2 cut(s) 192, 522
MaeII ACGT 1 cut(s) 1276
MaeIII GTNAC 2 cut(s) 1614, 1679
MfeI CAATTG 2 cut(s) 302, 626
MflI RGATCY 3 cut(s) 419, 746, 1468
MhlI GDGCHC 6 cut(s) 39, 301, 479, 1039, 1075, 1099
MlsI TGGCCA 2 cut(s) 1727, 1763
MluNI TGGCCA 2 cut(s) 1727, 1763
MlyI GAGTC 2 cut(s) 448, 596
MmeI TCCRAC 1 cut(s) 851
Mox20I TGGCCA 2 cut(s) 1727, 1763
MroXI GAANNNNTTC 2 cut(s) 604, 1646
MscI TGGCCA 2 cut(s) 1727, 1763
MseI TTAA 8 cut(s) 114, 951, 1115, 1155, 1184, 1245, 1662, 1698
Msp20I TGGCCA 2 cut(s) 1727, 1763
MspI CCGG 1 cut(s) 968
MspR9I CCNGG 1 cut(s) 1729
MunI CAATTG 2 cut(s) 302, 626
MvaI CCWGG 1 cut(s) 1729
MwoI GCNNNNNNNGC 2 cut(s) 159, 483
NdeI CATATG 1 cut(s) 1315
NlaIII CATG 2 cut(s) 1217, 1465
NlaIV GGNNCC 2 cut(s) 476, 795
NmuCI GTSAC 1 cut(s) 1679
NspI RCATGY 1 cut(s) 1217
PceI AGGCCT 1 cut(s) 659
PciI ACATGT 1 cut(s) 1213
PdmI GAANNNNTTC 2 cut(s) 604, 1646
PfeI GAWTC 1 cut(s) 605
PinAI ACCGGT 1 cut(s) 967
PkrI GCNGC 7 cut(s) 155, 158, 161, 178, 1321, 1713, 1739
PleI GAGTC 2 cut(s) 447, 595
PpsI GAGTC 2 cut(s) 447, 595
PscI ACATGT 1 cut(s) 1213
Psp6I CCWGG 1 cut(s) 1727
PspEI GGTNACC 1 cut(s) 1679
PspGI CCWGG 1 cut(s) 1727
PspN4I GGNNCC 2 cut(s) 476, 795
PspPI GGNCC 2 cut(s) 1542, 1627
PsuI RGATCY 3 cut(s) 419, 746, 1468
RsaI GTAC 2 cut(s) 972, 1337
RsaNI GTAC 2 cut(s) 971, 1336
SaqAI TTAA 8 cut(s) 114, 951, 1115, 1155, 1184, 1245, 1662, 1698
SatI GCNGC 7 cut(s) 154, 157, 160, 177, 1320, 1712, 1738
Sau96I GGNCC 2 cut(s) 1542, 1627
SchI GAGTC 2 cut(s) 448, 596
ScrFI CCNGG 1 cut(s) 1729
SduI GDGCHC 6 cut(s) 39, 301, 479, 1039, 1075, 1099
SfaNI GCATC 1 cut(s) 16
SinI GGWCC 2 cut(s) 1542, 1627
SmlI CTYRAG 1 cut(s) 1552
SmoI CTYRAG 1 cut(s) 1552
SseBI AGGCCT 1 cut(s) 659
SsiI CCGC 3 cut(s) 1177, 1379, 1706
SspMI CTAG 2 cut(s) 192, 522
StuI AGGCCT 1 cut(s) 659
StyD4I CCNGG 1 cut(s) 1727
TaaI ACNGT 2 cut(s) 53, 345
TaiI ACGT 1 cut(s) 1279
TaqI TCGA 2 cut(s) 369, 590
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 8 cut(s) 114, 951, 1115, 1155, 1184, 1245, 1662, 1698
Tru9I TTAA 8 cut(s) 114, 951, 1115, 1155, 1184, 1245, 1662, 1698
TscAI CASTG 5 cut(s) 58, 75, 237, 350, 1107
TseFI GTSAC 1 cut(s) 1679
TseI GCWGC 7 cut(s) 153, 156, 159, 176, 1319, 1711, 1737
Tsp45I GTSAC 1 cut(s) 1679
TspGWI ACGGA 1 cut(s) 1231
TspRI CASTG 5 cut(s) 58, 75, 237, 350, 1107
VpaK11BI GGWCC 2 cut(s) 1542, 1627
XapI RAATTY 3 cut(s) 139, 331, 1582
XceI RCATGY 1 cut(s) 1217
XcmI CCANNNNNNNNNTGG 2 cut(s) 1699, 1756
XmiI GTMKAC 1 cut(s) 1287
XmnI GAANNNNTTC 2 cut(s) 604, 1646
XspI CTAG 2 cut(s) 192, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.