RchiOBHm_Chr2g0099331

Ferredoxin-NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010146
Physical Location & Seq
Reverse (-)
11548381 .. 11551138
2758 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47405

Sequence Viewer

Length: 1092 bp
ATGGCTGCAGCTGTAACTGCTGCTGTTTCATTCCCATCATCCAAGTCCTCAACTCTCCCCACGAGAACATCCCTTATCTGTCCTGAAAGAGTCACCCTCAAGAAGGTTTATAGAGAAGCTTCTGCTGGTGGGAGAGTGGTTTCTGTCAGAGCCCAAGTCACCACCACTGAGGCTGCTCCACCTGCCAAGGCTGTGAAGCATTCCAAGAAGCAAGAGGAAGGTGTGGTTGTGAACAAGTTCAAGCCCAAGAACCCTTACGTTGGCAGAGTCCTCCTCAACACTAAGATCACTGCTGATGATGCACCTGGTGAGACCTGGCACATGGTCTTCAGCACTGATGGAGAGATGCCCTACAGAGAAGGACAGTCTATTGGAGTGATTCCAGATGGTATTGACCCTAAGAATGGCAAGCCTCACAAGCTCAGGCTGTACTCAATTGCCAGTAGTGCCCTTGGTGACTTTGGTGACTCCAAAACTGTTTCTCTCTGTGTGAAAAGGCTTGTGTACACCAATGACGCAGGCGAAACCGTTAAAGGGGTGTGCTCAAACTTTTTGTGTGACTTGAAGCCAGGGAGTGAAGTGACCATGACAGGACCAGTTGGCAAAGAAATGCTTATGCCAAAAGATCCTAATGCAACCGTCGTCATGCTTGCAACTGGTACTGGAATTGCTCCTTTCAGATCATTCTTGTGGAAAATGTTCTTTGAGAAGCATGAAGACTACAAGTTTGAAGGTTTGGCTTGGCTCTTCTTGGGTGTTCCCACCAGTAGCTCACTGCTTTACAAGGAGGAATTTGAGAAGATGAAAGAGAAGGCCCCCGATAACTTCAGGCTTGACTTCGCTGTCAGCAGAGAGCAAACCAACGAGAAGGGCGAGAAAATGTACATTCAAACCAGAATGGCTCAGTATGCAGAAGAGCTATGGGAGTTACTCAAGAAGGACAACACCTACGTCTACATGTGCGGTCTCAAGGGAATGGAGAAGGGAATTGATGACATCATGGTGTCATTGGCTGCGAAAGACGGCATTGACTGGTTAGACTACAAAAAGCAGTTGAAGAAAGCAGAGCAATGGAATGTGGAAGTCTACTAA

Protein Analysis

363

Amino Acids

40.39

Weight (kDa)

8.69

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 216 - 330 1.9e-27 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20020 AT1G20020 AT1G20020 AT5G66190 AT5G66190
fragaria_vesca FvH4_1g11730
malus_domestica MD02G1130800.v1.1 MD15G1244500.v1.1
prunus_persica Prupe.7G169200_v2.0.a1
pyrus_communis pycom02g10250 pycom15g21530
rosa_chinensis RchiOBHm_Chr2g0099331
rosa_laevigata RLG00000016872
rosa_multiflora Rmu_sc0009774.1_g000008
rosa_roxburghii Rroxscaffold_2G00143200
rosa_rugosa Rorug02G0078700
rosa_samantha Rh2AG128200 Rh2BG131100 Rh2CG132300 Rh2DG132700
rosa_wichuraiana Rw2G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
AasI GACNNNNNNGTC 1 cut(s) 842
Acc36I ACCTGC 1 cut(s) 190
AccI GTMKAC 2 cut(s) 954, 1086
AciI CCGC 1 cut(s) 963
AclWI GGATC 1 cut(s) 620
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 2 cut(s) 313, 811
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 4 cut(s) 431, 506, 661, 884
AfiI CCNNNNNNNGG 4 cut(s) 103, 260, 404, 534
AflIII ACRYGT 1 cut(s) 957
AgsI TTSAA 5 cut(s) 241, 565, 731, 890, 1057
AjnI CCWGG 3 cut(s) 304, 314, 568
AluBI AGCT 5 cut(s) 11, 119, 421, 771, 919
AluI AGCT 5 cut(s) 11, 119, 421, 771, 919
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 2 cut(s) 305, 971
AlwI GGATC 1 cut(s) 620
AoxI GGCC 1 cut(s) 813
ApeKI GCWGC 5 cut(s) 5, 8, 20, 173, 1013
ApoI RAATTY 1 cut(s) 791
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
Asp700I GAANNNNTTC 2 cut(s) 236, 698
AspS9I GGNCC 2 cut(s) 593, 814
AsuHPI GGTGA 5 cut(s) 85, 151, 320, 467, 476
AvaII GGWCC 1 cut(s) 593
BaeGI GKGCMC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 154
BauI CACGAG 1 cut(s) 61
BbsI GAAGAC 2 cut(s) 319, 723
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 4 cut(s) 7, 20, 160, 1000
BccI CCATC 3 cut(s) 43, 332, 380
BceAI ACGGC 1 cut(s) 1039
BciT130I CCWGG 3 cut(s) 306, 316, 570
BcoDI GTCTC 2 cut(s) 305, 971
BfmI CTRYAG 2 cut(s) 6, 352
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 5 cut(s) 6, 9, 21, 174, 1014
BlsI GCNGC 5 cut(s) 7, 10, 22, 175, 1015
Bme1390I CCNGG 3 cut(s) 306, 316, 570
Bme18I GGWCC 1 cut(s) 593
BmgT120I GGNCC 2 cut(s) 593, 814
BmiI GGNNCC 1 cut(s) 816
BmrFI CCNGG 3 cut(s) 306, 316, 570
BmsI GCATC 2 cut(s) 289, 336
BpiI GAAGAC 2 cut(s) 319, 723
BplI GAGNNNNNCTC 4 cut(s) 81, 113, 258, 290
Bpu10I CCTNAGC 1 cut(s) 422
BpuEI CTTGAG 3 cut(s) 83, 917, 953
BsaI GGTCTC 2 cut(s) 305, 971
BsaJI CCNNGG 3 cut(s) 186, 451, 569
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 260, 404, 534
Bse1I ACTGG 6 cut(s) 441, 596, 661, 667, 765, 1037
Bse3DI GCAATG 1 cut(s) 1076
BseBI CCWGG 3 cut(s) 306, 316, 570
BseDI CCNNGG 3 cut(s) 186, 451, 569
BseGI GGATG 2 cut(s) 38, 68
BseLI CCNNNNNNNGG 4 cut(s) 103, 260, 404, 534
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 3 cut(s) 159, 436, 917
BseNI ACTGG 6 cut(s) 441, 596, 661, 667, 765, 1037
BseRI GAGGAG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 4 cut(s) 7, 20, 160, 1000
BshFI GGCC 1 cut(s) 815
BsiHKAI GWGCWC 1 cut(s) 545
BslI CCNNNNNNNGG 4 cut(s) 103, 260, 404, 534
BsmAI GTCTC 2 cut(s) 305, 971
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 815
Bso31I GGTCTC 2 cut(s) 305, 971
Bsp1286I GDGCHC 3 cut(s) 154, 451, 545
Bsp1407I TGTACA 2 cut(s) 504, 882
Bsp143I GATC 3 cut(s) 285, 625, 680
BspACI CCGC 1 cut(s) 963
BspANI GGCC 1 cut(s) 815
BspCNI CTCAG 3 cut(s) 160, 435, 916
BspLI GGNNCC 1 cut(s) 816
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 2 cut(s) 752, 909
BspTNI GGTCTC 2 cut(s) 305, 971
BsrDI GCAATG 1 cut(s) 1076
BsrGI TGTACA 2 cut(s) 504, 882
BsrI ACTGG 6 cut(s) 441, 596, 661, 667, 765, 1037
BssECI CCNNGG 3 cut(s) 186, 451, 569
BssMI GATC 3 cut(s) 285, 625, 680
BssSI CACGAG 1 cut(s) 61
BssT1I CCWWGG 2 cut(s) 186, 451
Bst2BI CACGAG 1 cut(s) 61
Bst2UI CCWGG 3 cut(s) 306, 316, 570
Bst4CI ACNGT 4 cut(s) 366, 478, 529, 640
Bst6I CTCTTC 2 cut(s) 752, 909
BstAUI TGTACA 2 cut(s) 504, 882
BstC8I GCNNGC 3 cut(s) 410, 520, 651
BstDEI CTNAG 5 cut(s) 168, 282, 399, 422, 903
BstENI CCTNNNNNAGG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 38, 68
BstKTI GATC 3 cut(s) 288, 628, 683
BstMAI GTCTC 2 cut(s) 305, 971
BstMBI GATC 3 cut(s) 285, 625, 680
BstMWI GCNNNNNNNGC 6 cut(s) 17, 182, 299, 418, 446, 908
BstNI CCWGG 3 cut(s) 306, 316, 570
BstNSI RCATGY 1 cut(s) 961
BstSCI CCNGG 3 cut(s) 304, 314, 568
BstSFI CTRYAG 2 cut(s) 6, 352
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 4 cut(s) 7, 20, 160, 1000
BstV2I GAAGAC 2 cut(s) 319, 723
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 1 cut(s) 815
BtsCI GGATG 2 cut(s) 38, 68
BtsI GCAGTG 2 cut(s) 288, 773
BtsIMutI CAGTG 4 cut(s) 165, 288, 333, 773
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 3 cut(s) 410, 520, 651
Cfr13I GGNCC 2 cut(s) 593, 814
CseI GACGC 1 cut(s) 524
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 4 cut(s) 430, 505, 660, 883
CviAII CATG 6 cut(s) 322, 586, 646, 713, 958, 1000
CviQI GTAC 4 cut(s) 430, 505, 660, 883
DdeI CTNAG 5 cut(s) 168, 282, 399, 422, 903
DpnI GATC 3 cut(s) 287, 627, 682
DpnII GATC 3 cut(s) 285, 625, 680
DraIII CACNNNGTG 1 cut(s) 308
DrdI GACNNNNNNGTC 1 cut(s) 842
DseDI GACNNNNNNGTC 1 cut(s) 842
Eam1104I CTCTTC 2 cut(s) 752, 909
EarI CTCTTC 2 cut(s) 752, 909
Eco130I CCWWGG 2 cut(s) 186, 451
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 2 cut(s) 305, 971
Eco47I GGWCC 1 cut(s) 593
Eco57I CTGAAG 2 cut(s) 313, 811
EcoNI CCTNNNNNAGG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 814
EcoRII CCWGG 3 cut(s) 304, 314, 568
EcoT14I CCWWGG 2 cut(s) 186, 451
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 2 cut(s) 186, 451
FaeI CATG 6 cut(s) 325, 589, 649, 716, 961, 1003
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FatI CATG 6 cut(s) 321, 585, 645, 712, 957, 999
FblI GTMKAC 2 cut(s) 954, 1086
Fnu4HI GCNGC 5 cut(s) 6, 9, 21, 174, 1014
FokI GGATG 2 cut(s) 25, 55
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 5 cut(s) 6, 9, 21, 174, 1014
GluI GCNGC 5 cut(s) 6, 9, 21, 174, 1014
HaeIII GGCC 1 cut(s) 815
HgaI GACGC 1 cut(s) 524
Hin1II CATG 6 cut(s) 325, 589, 649, 716, 961, 1003
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 4 cut(s) 90, 267, 379, 467
HphI GGTGA 5 cut(s) 85, 151, 320, 467, 476
Hpy166II GTNNAC 5 cut(s) 232, 505, 507, 955, 1087
Hpy188I TCNGA 2 cut(s) 149, 680
Hpy188III TCNNGA 4 cut(s) 83, 100, 383, 934
Hpy8I GTNNAC 5 cut(s) 232, 505, 507, 955, 1087
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 8 cut(s) 97, 212, 353, 725, 805, 862, 931, 976
HpyCH4III ACNGT 4 cut(s) 366, 478, 529, 640
HpyCH4IV ACGT 2 cut(s) 258, 951
HpyCH4V TGCA 5 cut(s) 8, 302, 635, 653, 911
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 182, 299, 418, 446, 908
HpyF3I CTNAG 5 cut(s) 168, 282, 399, 422, 903
HpySE526I ACGT 2 cut(s) 258, 951
Hsp92II CATG 6 cut(s) 325, 589, 649, 716, 961, 1003
Kzo9I GATC 3 cut(s) 285, 625, 680
LguI GCTCTTC 2 cut(s) 752, 909
LmnI GCTCC 2 cut(s) 181, 676
Lsp1109I GCAGC 4 cut(s) 7, 20, 160, 1000
LweI GCATC 2 cut(s) 289, 336
MabI ACCWGGT 1 cut(s) 304
MaeII ACGT 2 cut(s) 258, 951
MaeIII GTNAC 8 cut(s) 13, 91, 157, 455, 464, 557, 580, 927
MalI GATC 3 cut(s) 287, 627, 682
MboI GATC 3 cut(s) 285, 625, 680
MboII GAAGA 6 cut(s) 319, 728, 739, 811, 926, 1069
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 3 cut(s) 154, 451, 545
MluCI AATT 4 cut(s) 435, 666, 791, 987
MlyI GAGTC 3 cut(s) 99, 276, 461
MnlI CCTC 8 cut(s) 58, 107, 163, 208, 281, 284, 423, 781
MroXI GAANNNNTTC 2 cut(s) 236, 698
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 2 cut(s) 688, 1001
MspA1I CMGCKG 1 cut(s) 11
MspR9I CCNGG 3 cut(s) 306, 316, 570
MunI CAATTG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 3 cut(s) 306, 316, 570
MwoI GCNNNNNNNGC 6 cut(s) 17, 182, 299, 418, 446, 908
NdeII GATC 3 cut(s) 285, 625, 680
NlaIII CATG 6 cut(s) 325, 589, 649, 716, 961, 1003
NlaIV GGNNCC 1 cut(s) 816
NmuCI GTSAC 6 cut(s) 91, 157, 455, 464, 557, 580
NspI RCATGY 1 cut(s) 961
PaqCI CACCTGC 1 cut(s) 190
PciI ACATGT 1 cut(s) 957
PciSI GCTCTTC 2 cut(s) 752, 909
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 2 cut(s) 236, 698
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 5 cut(s) 7, 10, 22, 175, 1015
PleI GAGTC 3 cut(s) 98, 275, 461
PpsI GAGTC 3 cut(s) 98, 275, 461
PscI ACATGT 1 cut(s) 957
Psp6I CCWGG 3 cut(s) 304, 314, 568
PspGI CCWGG 3 cut(s) 304, 314, 568
PspN4I GGNNCC 1 cut(s) 816
PspPI GGNCC 2 cut(s) 593, 814
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 4 cut(s) 431, 506, 661, 884
RsaNI GTAC 4 cut(s) 430, 505, 660, 883
RseI CAYNNNNRTG 2 cut(s) 688, 1001
SapI GCTCTTC 2 cut(s) 752, 909
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 5 cut(s) 6, 9, 21, 174, 1014
Sau3AI GATC 3 cut(s) 285, 625, 680
Sau96I GGNCC 2 cut(s) 593, 814
SchI GAGTC 3 cut(s) 99, 276, 461
ScrFI CCNGG 3 cut(s) 306, 316, 570
SduI GDGCHC 3 cut(s) 154, 451, 545
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 2 cut(s) 289, 336
SfcI CTRYAG 2 cut(s) 6, 352
SinI GGWCC 1 cut(s) 593
SmiMI CAYNNNNRTG 2 cut(s) 688, 1001
SmlI CTYRAG 3 cut(s) 98, 932, 968
SmoI CTYRAG 3 cut(s) 98, 932, 968
Sse9I AATT 4 cut(s) 435, 666, 791, 987
SsiI CCGC 1 cut(s) 963
StyD4I CCNGG 3 cut(s) 304, 314, 568
StyI CCWWGG 2 cut(s) 186, 451
TaaI ACNGT 4 cut(s) 366, 478, 529, 640
TaiI ACGT 2 cut(s) 261, 954
TasI AATT 4 cut(s) 435, 666, 791, 987
TatI WGTACW 3 cut(s) 429, 504, 882
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 4 cut(s) 172, 295, 340, 780
TseFI GTSAC 6 cut(s) 91, 157, 455, 464, 557, 580
TseI GCWGC 5 cut(s) 5, 8, 20, 173, 1013
Tsp45I GTSAC 6 cut(s) 91, 157, 455, 464, 557, 580
TspDTI ATGAA 3 cut(s) 18, 729, 818
TspRI CASTG 4 cut(s) 172, 295, 340, 780
VpaK11BI GGWCC 1 cut(s) 593
XagI CCTNNNNNAGG 1 cut(s) 101
XapI RAATTY 1 cut(s) 791
XceI RCATGY 1 cut(s) 961
XmiI GTMKAC 2 cut(s) 954, 1086
XmnI GAANNNNTTC 2 cut(s) 236, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.