RchiOBHm_Chr2g0130551

Aspartate aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010223
Physical Location & Seq
Reverse (-)
46580996 .. 46587003
6008 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50201

Sequence Viewer

Length: 1269 bp
ATGGCGCTCTCCACCAAACTTTTTCAACGCAGCAGCTCCACCGCAGTCTTTGGCGCGCGATCCATGTCCTGGTGGCGGAGTGTCCAACCGGCTCCCAAAGATCCCATTCTCGGCGTCACCGAAGCCTTCCTCGCCGATCCCAGTCCCAACAAAGTCAATGTTGGAGTCGGTGCGTACCGTGATGACAATGGAAAGCCGGTTGTTCTGGAATGCGTTAGAGAAGCGGAGCGGAGGGTTGCCGGAAAATTGAACATGGAATATCTTCCTATGGGGGGCAGTGTGAAGATGGTGGAAGAAACATTGAAGCTGGCCTATGGAGAGACTTCTGAATTTCTCAAAGATAAAAGGATAGCAGCAGTGCAATCTCTCTCTGGGACTGGTGCATGTCGTCTTTTTGCTGACTTTCAAAAACGTTTTCGTCCAGATTCTAATATCTATATTCCAGTGCCAACATGGGCCAACCACCATAACATCTGGAGAGATGCCCAGGTCCCTCAAAGGACTTTCCATTACTATCATCCAGAGACTAAGGGGTTGGACTTTGCAGGACTGATTGATGACATAAAGAATGCTCCAAGTGGTTCATTCTTTCTACTTCATGCATGTGCTCACAATCCCACTGGAGTTGATCCTTCGGAGGAACAATGGAAAGAGATCTCATACGAAATCAAGGGTAAAGGTCATTTTGCCTTCTTTGACATGGCATATCAAGGTTTTGCTAGTGGTGACCCAGAGAGAGATGCAAAAGCCATCAGGATTTTTCTCAAGGATGGTCATCTAATTGGAATTTCTCAATCTTATGCAAAAAATATGGGACTCTATGGTCAGAGAGTAGGATGCCTTAGCTTGCTTTGTGAGGATGAAAAACAAGCTGTGGCTGTAAAAAGTCAGTTGCAGCAGCTTGCCAGACCCATGTATAGTAACCCACCTGTTCATGGACCTCTTATAGTGTCAACTATTCTTGGTGATCCAGACTTGAAGAATTTGTGGCTCCGAGAAGTTAAGGGCATGGCAGATCGCATTATCGGAATGCGAACTGCTCTACGAGAAAATCTTGAGAAATTGGGATCACCCATATCTTGGGAGCACATAACAAATCAGATTGGCATGTTTTGCTATAGCGGAATGACACCTGAACAGGTTGATCGTTTGACAAATGAGTTTCATATATATTTGACTCGCAATGGTCGGATAAGTATGGCGGGTGTTACAACGGGCAATGTTGGATATCTGGCAAATGCTATACATGAAGTAACAAAATCTGCATAG

Protein Analysis

422

Amino Acids

46.97

Weight (kDa)

8.39

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 50 - 415 1e-90 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 822
AccB7I CCANNNNNTGG 2 cut(s) 69, 1080
AccBSI CCGCTC 1 cut(s) 229
AccII CGCG 2 cut(s) 56, 58
AciI CCGC 6 cut(s) 42, 76, 224, 229, 1122, 1202
AclI AACGTT 1 cut(s) 412
AclWI GGATC 6 cut(s) 54, 95, 131, 623, 962, 1075
AcsI RAATTY 3 cut(s) 329, 786, 982
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 935, 1080
AgsI TTSAA 5 cut(s) 26, 250, 304, 407, 979
AjnI CCWGG 2 cut(s) 68, 486
AluBI AGCT 5 cut(s) 36, 307, 846, 872, 901
AluI AGCT 5 cut(s) 36, 307, 846, 872, 901
Alw21I GWGCWC 2 cut(s) 610, 1089
Alw26I GTCTC 2 cut(s) 314, 518
AlwI GGATC 6 cut(s) 54, 95, 131, 623, 962, 1075
AoxI GGCC 2 cut(s) 309, 456
ApeKI GCWGC 5 cut(s) 30, 33, 353, 895, 898
ApoI RAATTY 3 cut(s) 329, 786, 982
Asp700I GAANNNNTTC 1 cut(s) 261
AspLEI GCGC 3 cut(s) 7, 56, 58
AspS9I GGNCC 3 cut(s) 456, 490, 938
AsuHPI GGTGA 4 cut(s) 109, 737, 977, 1062
AvaII GGWCC 2 cut(s) 490, 938
Bbv12I GWGCWC 2 cut(s) 610, 1089
BbvI GCAGC 5 cut(s) 42, 45, 365, 907, 910
BccI CCATC 3 cut(s) 280, 758, 764
BciT130I CCWGG 2 cut(s) 70, 488
BcoDI GTCTC 2 cut(s) 314, 518
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 1 cut(s) 1117
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 654
BisI GCNGC 5 cut(s) 31, 34, 354, 896, 899
BlsI GCNGC 5 cut(s) 32, 35, 355, 897, 900
Bme1390I CCNGG 2 cut(s) 70, 488
Bme18I GGWCC 2 cut(s) 490, 938
BmgT120I GGNCC 3 cut(s) 456, 490, 938
BmiI GGNNCC 3 cut(s) 93, 492, 992
BmrFI CCNGG 2 cut(s) 70, 488
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 3 cut(s) 472, 730, 827
BmuI ACTGGG 1 cut(s) 135
BpmI CTGGAG 2 cut(s) 496, 642
Bpu10I CCTNAGC 1 cut(s) 842
BpuEI CTTGAG 2 cut(s) 749, 1076
BsaBI GATNNNNATC 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 486
Bsc4I CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 935, 1080
Bse118I RCCGGY 2 cut(s) 88, 196
Bse1I ACTGG 4 cut(s) 141, 382, 443, 625
Bse3DI GCAATG 2 cut(s) 1189, 1225
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 2 cut(s) 70, 488
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 4 cut(s) 517, 775, 842, 865
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 935, 1080
BseMI GCAATG 2 cut(s) 1189, 1225
BseNI ACTGG 4 cut(s) 141, 382, 443, 625
BsePI GCGCGC 1 cut(s) 54
BseXI GCAGC 5 cut(s) 42, 45, 365, 907, 910
Bsh1236I CGCG 2 cut(s) 56, 58
BshFI GGCC 2 cut(s) 311, 458
BsiHKAI GWGCWC 2 cut(s) 610, 1089
BsiSI CCGG 3 cut(s) 89, 197, 240
BslFI GGGAC 4 cut(s) 129, 388, 476, 828
BslI CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 935, 1080
BsmAI GTCTC 2 cut(s) 314, 518
BsmFI GGGAC 4 cut(s) 129, 388, 476, 828
BsmI GAATGC 3 cut(s) 215, 574, 1035
BsnI GGCC 2 cut(s) 311, 458
Bsp1286I GDGCHC 2 cut(s) 610, 1089
Bsp143I GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
BspACI CCGC 6 cut(s) 42, 76, 224, 229, 1122, 1202
BspANI GGCC 2 cut(s) 311, 458
BspFNI CGCG 2 cut(s) 56, 58
BspLI GGNNCC 3 cut(s) 93, 492, 992
BspPI GGATC 6 cut(s) 54, 95, 131, 623, 962, 1075
BsrBI CCGCTC 1 cut(s) 229
BsrDI GCAATG 2 cut(s) 1189, 1225
BsrFI RCCGGY 2 cut(s) 88, 196
BsrI ACTGG 4 cut(s) 141, 382, 443, 625
BssAI RCCGGY 2 cut(s) 88, 196
BssECI CCNNGG 1 cut(s) 486
BssHII GCGCGC 1 cut(s) 54
BssMI GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
BssNI GRCGYC 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 70, 488
Bst4CI ACNGT 1 cut(s) 179
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 1113
BstC8I GCNNGC 4 cut(s) 56, 309, 848, 903
BstDEI CTNAG 2 cut(s) 528, 842
BstEII GGTNACC 1 cut(s) 725
BstF5I GGATG 4 cut(s) 517, 775, 842, 865
BstFNI CGCG 2 cut(s) 56, 58
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 56, 58
BstKTI GATC 9 cut(s) 62, 103, 139, 631, 657, 970, 1018, 1070, 1147
BstMAI GTCTC 2 cut(s) 314, 518
BstMBI GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 131, 1113
BstNI CCWGG 2 cut(s) 70, 488
BstNSI RCATGY 3 cut(s) 387, 606, 1111
BstPI GGTNACC 1 cut(s) 725
BstSCI CCNGG 2 cut(s) 68, 486
BstSFI CTRYAG 1 cut(s) 1117
BstUI CGCG 2 cut(s) 56, 58
BstV1I GCAGC 5 cut(s) 42, 45, 365, 907, 910
BstX2I RGATCY 2 cut(s) 100, 654
BstYI RGATCY 2 cut(s) 100, 654
BsuRI GGCC 2 cut(s) 311, 458
BtsCI GGATG 4 cut(s) 517, 775, 842, 865
BtsI GCAGTG 2 cut(s) 283, 363
BtsIMutI CAGTG 4 cut(s) 283, 363, 450, 618
Cac8I GCNNGC 4 cut(s) 56, 309, 848, 903
CfoI GCGC 3 cut(s) 7, 56, 58
Cfr10I RCCGGY 2 cut(s) 88, 196
Cfr13I GGNCC 3 cut(s) 456, 490, 938
CseI GACGC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 175
CspCI CAANNNNNGTGG 2 cut(s) 607, 642
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 2 cut(s) 528, 842
DpnI GATC 9 cut(s) 61, 102, 138, 630, 656, 969, 1017, 1069, 1146
DpnII GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
DrdI GACNNNNNNGTC 1 cut(s) 822
DseDI GACNNNNNNGTC 1 cut(s) 822
EciI GGCGGA 1 cut(s) 91
Eco32I GATATC 1 cut(s) 1229
Eco47I GGWCC 2 cut(s) 490, 938
Eco91I GGTNACC 1 cut(s) 725
EcoO109I RGGNCCY 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 68, 486
EcoRV GATATC 1 cut(s) 1229
EcoT22I ATGCAT 1 cut(s) 604
FaqI GGGAC 4 cut(s) 129, 388, 476, 828
FauI CCCGC 1 cut(s) 1195
Fnu4HI GCNGC 5 cut(s) 31, 34, 354, 896, 899
FokI GGATG 4 cut(s) 504, 782, 849, 872
Fsp4HI GCNGC 5 cut(s) 31, 34, 354, 896, 899
FspBI CTAG 1 cut(s) 720
GlaI GCGC 3 cut(s) 6, 55, 57
GluI GCNGC 5 cut(s) 31, 34, 354, 896, 899
GsuI CTGGAG 2 cut(s) 496, 642
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 311, 458
HapII CCGG 3 cut(s) 89, 197, 240
HgaI GACGC 1 cut(s) 103
HhaI GCGC 3 cut(s) 7, 56, 58
Hin1I GRCGYC 1 cut(s) 114
Hin6I GCGC 3 cut(s) 5, 54, 56
HinP1I GCGC 3 cut(s) 5, 54, 56
HincII GTYRAC 1 cut(s) 954
HindII GTYRAC 1 cut(s) 954
HinfI GANTC 4 cut(s) 165, 425, 816, 1177
HpaII CCGG 3 cut(s) 89, 197, 240
HphI GGTGA 4 cut(s) 109, 737, 977, 1062
Hpy166II GTNNAC 1 cut(s) 954
Hpy188I TCNGA 7 cut(s) 328, 637, 828, 995, 1028, 1101, 1191
Hpy188III TCNNGA 7 cut(s) 206, 422, 475, 521, 754, 971, 1055
Hpy8I GTNNAC 1 cut(s) 954
HpyAV CCTTC 3 cut(s) 136, 642, 700
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 8 cut(s) 361, 383, 545, 602, 743, 803, 895, 1265
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 1113
HpyF3I CTNAG 2 cut(s) 528, 842
HpySE526I ACGT 1 cut(s) 412
Hsp92I GRCGYC 1 cut(s) 114
HspAI GCGC 3 cut(s) 5, 54, 56
Kzo9I GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
LmnI GCTCC 6 cut(s) 41, 97, 226, 577, 996, 1084
Lsp1109I GCAGC 5 cut(s) 42, 45, 365, 907, 910
LweI GCATC 3 cut(s) 472, 730, 827
MaeI CTAG 1 cut(s) 720
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 5 cut(s) 115, 725, 920, 1207, 1252
MalI GATC 9 cut(s) 61, 102, 138, 630, 656, 969, 1017, 1069, 1146
MbiI CCGCTC 1 cut(s) 229
MboI GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
MboII GAAGA 4 cut(s) 254, 295, 305, 991
MflI RGATCY 2 cut(s) 100, 654
MhlI GDGCHC 2 cut(s) 610, 1089
MluCI AATT 6 cut(s) 245, 329, 780, 786, 982, 1061
MlyI GAGTC 3 cut(s) 174, 810, 1171
MmeI TCCRAC 5 cut(s) 109, 142, 516, 1169, 1204
MnlI CCTC 6 cut(s) 140, 225, 504, 631, 850, 951
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 1 cut(s) 1002
MslI CAYNNNNRTG 1 cut(s) 603
MspI CCGG 3 cut(s) 89, 197, 240
MspR9I CCNGG 2 cut(s) 70, 488
Mva1269I GAATGC 3 cut(s) 215, 574, 1035
MvaI CCWGG 2 cut(s) 70, 488
MvnI CGCG 2 cut(s) 56, 58
MwoI GCNNNNNNNGC 2 cut(s) 131, 1113
NdeII GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
NlaIV GGNNCC 3 cut(s) 93, 492, 992
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 2 cut(s) 115, 725
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 3 cut(s) 387, 606, 1111
PauI GCGCGC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 3 cut(s) 215, 574, 1035
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 425
PflMI CCANNNNNTGG 2 cut(s) 69, 1080
PkrI GCNGC 5 cut(s) 32, 35, 355, 897, 900
PleI GAGTC 3 cut(s) 173, 810, 1171
PpsI GAGTC 3 cut(s) 173, 810, 1171
PpuMI RGGWCCY 1 cut(s) 490
Psp1406I AACGTT 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 2 cut(s) 68, 486
PspEI GGTNACC 1 cut(s) 725
PspGI CCWGG 2 cut(s) 68, 486
PspN4I GGNNCC 3 cut(s) 93, 492, 992
PspPI GGNCC 3 cut(s) 456, 490, 938
PspPPI RGGWCCY 1 cut(s) 490
PsuI RGATCY 2 cut(s) 100, 654
PteI GCGCGC 1 cut(s) 54
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 1 cut(s) 603
SaqAI TTAA 1 cut(s) 1002
SatI GCNGC 5 cut(s) 31, 34, 354, 896, 899
Sau3AI GATC 9 cut(s) 59, 100, 136, 628, 654, 967, 1015, 1067, 1144
Sau96I GGNCC 3 cut(s) 456, 490, 938
SchI GAGTC 3 cut(s) 174, 810, 1171
ScrFI CCNGG 2 cut(s) 70, 488
SduI GDGCHC 2 cut(s) 610, 1089
SfaNI GCATC 3 cut(s) 472, 730, 827
SfcI CTRYAG 1 cut(s) 1117
SinI GGWCC 2 cut(s) 490, 938
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 764, 1055
SmoI CTYRAG 2 cut(s) 764, 1055
Sse9I AATT 6 cut(s) 245, 329, 780, 786, 982, 1061
SsiI CCGC 6 cut(s) 42, 76, 224, 229, 1122, 1202
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 68, 486
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 1 cut(s) 415
TasI AATT 6 cut(s) 245, 329, 780, 786, 982, 1061
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 1 cut(s) 1002
Tru9I TTAA 1 cut(s) 1002
TscAI CASTG 4 cut(s) 283, 363, 450, 625
TseFI GTSAC 2 cut(s) 115, 725
TseI GCWGC 5 cut(s) 30, 33, 353, 895, 898
Tsp45I GTSAC 2 cut(s) 115, 725
TspDTI ATGAA 6 cut(s) 573, 587, 876, 923, 1154, 1263
TspRI CASTG 4 cut(s) 283, 363, 450, 625
Van91I CCANNNNNTGG 2 cut(s) 69, 1080
VpaK11BI GGWCC 2 cut(s) 490, 938
XapI RAATTY 3 cut(s) 329, 786, 982
XceI RCATGY 3 cut(s) 387, 606, 1111
XcmI CCANNNNNNNNNTGG 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.