Rh2AG343500

Aspartate aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010223
Physical Location & Seq
Reverse (-)
50491416 .. 50497442
6027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG343500.1

Sequence Viewer

Length: 1107 bp
ATGGCGCTCTCCACCAAACTTTTTCAACGCAGGAGCTCCACCGCAGTCTTGGGCGCGCGATCCATGTCCTGGTGGCGGACTGTCCAACCGGCTCCCAAAGATCCCATTCTCGGCGTCACCGAAGCCTTCCTCGCCGATCCCAGTCCCAACAAAGTCAATGTTGGAGTCGGTGCGTACCGTGATGACAATGGAAAGCCGGTTGTTTTGGAATGCGTTAGAGAAGCGGAGCGGAGGGTTGCCGGAAAATTGAACATGGAATATCTTCCTATGGGGGGCAGTGTGAAGATGGTGGAAGAAACATTGAAGCTGGCCTATGGAGAGACTTCTGAATTTCTCAAAGATAAAAGGATAGCAGCAGTGCAATCTCTCTCTGGGACTGGTGCATGTCGTCTTTTTGCTGACTTTCAAAAACGTTTTCGTCCAGATTCTAATATCTATATTCCAGTGCCAACATGGGCCAACCACCATAACATCTGGAGAGATGCCCAGGTCCCTCAAAGGACTTTCCATTACTATCATCCAGAGACTAAGGGTTTGGACTTTGCAGGACTGATTGATGACATAAAGAATGCTCCAAGTGGCTCATTCTTTCTACTTCATGCATGTGCTCACAATCCCACTGGAGTTGATCCTTCGGAGGAACAATGGAAGGAGATCTCGTACCAAATCAAGGGTAAAGGTCATTTTGCCTTCTTTGACATGGCATATCAAGGTTTTGCTAGTGGTAACCCAGAGAGAGATGCAAAAGCCATCAGGATTTTTCTCGAGGATGGTCATCTAATTGGAATTTCTCAATCTTATGCAAAAAATATGGGACTCTATGGTCAGAGAGTAGGATGCCTTAGCTTGCTTTGTGAAGATGAAAAACAAGCTGTGGCTGTAAAAAGTCAGTTGCAGCAGCTTGCCAGACCCATGTATAGTAACCCACCCGTTCATGGACCGCTTATAGTGTCAACTATCCTTGGTGATCCAGACTTGAAGAATTTGTGGCTCCGAGAAGTTAAGGTAATGTGTGGTTATTTCCTTTCAACTTTATATCTTGGGATGACTAATGTGATTACTAACCAAGTACGTGGTAAGAGAACCCTTGGGATGAGCATAAAGTGA

Protein Analysis

368

Amino Acids

41.06

Weight (kDa)

9.07

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 50 - 333 1.6e-69 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 822
AccB7I CCANNNNNTGG 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 229
AccII CGCG 2 cut(s) 56, 58
AciI CCGC 5 cut(s) 42, 76, 224, 229, 941
AclI AACGTT 1 cut(s) 412
AclWI GGATC 5 cut(s) 54, 95, 131, 623, 962
AcsI RAATTY 3 cut(s) 329, 786, 982
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 176, 662, 1071
AfiI CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 670, 935
AgsI TTSAA 6 cut(s) 26, 250, 304, 407, 979, 1029
AjnI CCWGG 2 cut(s) 68, 486
AluBI AGCT 5 cut(s) 36, 307, 846, 872, 901
AluI AGCT 5 cut(s) 36, 307, 846, 872, 901
Alw21I GWGCWC 2 cut(s) 38, 610
Alw26I GTCTC 2 cut(s) 314, 518
AlwI GGATC 5 cut(s) 54, 95, 131, 623, 962
Ama87I CYCGRG 1 cut(s) 764
AoxI GGCC 2 cut(s) 309, 456
ApeKI GCWGC 3 cut(s) 353, 895, 898
ApoI RAATTY 3 cut(s) 329, 786, 982
ArsI GACNNNNNNTTYG 2 cut(s) 517, 549
Asp700I GAANNNNTTC 1 cut(s) 261
AspLEI GCGC 3 cut(s) 7, 56, 58
AspS9I GGNCC 3 cut(s) 456, 490, 938
AsuHPI GGTGA 2 cut(s) 109, 977
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 2 cut(s) 490, 938
BanII GRGCYC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 38, 610
BbvI GCAGC 3 cut(s) 365, 907, 910
BccI CCATC 3 cut(s) 280, 758, 764
BciT130I CCWGG 2 cut(s) 70, 488
BcoDI GTCTC 2 cut(s) 314, 518
BfaI CTAG 1 cut(s) 720
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 654
BisI GCNGC 3 cut(s) 354, 896, 899
BlsI GCNGC 3 cut(s) 355, 897, 900
Bme1390I CCNGG 2 cut(s) 70, 488
Bme18I GGWCC 2 cut(s) 490, 938
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 3 cut(s) 456, 490, 938
BmiI GGNNCC 3 cut(s) 93, 492, 992
BmrFI CCNGG 2 cut(s) 70, 488
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 3 cut(s) 472, 730, 827
BmuI ACTGGG 1 cut(s) 135
BpmI CTGGAG 2 cut(s) 496, 642
Bpu10I CCTNAGC 1 cut(s) 842
BsaAI YACGTR 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 486, 961, 1087
BsaXI ACNNNNNCTCC 2 cut(s) 644, 674
Bsc4I CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 670, 935
Bse118I RCCGGY 2 cut(s) 88, 196
Bse1I ACTGG 4 cut(s) 141, 382, 443, 625
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 2 cut(s) 70, 488
BseDI CCNNGG 3 cut(s) 486, 961, 1087
BseGI GGATG 5 cut(s) 517, 775, 842, 1050, 1098
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 670, 935
BseNI ACTGG 4 cut(s) 141, 382, 443, 625
BsePI GCGCGC 1 cut(s) 54
BseXI GCAGC 3 cut(s) 365, 907, 910
Bsh1236I CGCG 2 cut(s) 56, 58
BshFI GGCC 2 cut(s) 311, 458
BsiHKAI GWGCWC 2 cut(s) 38, 610
BsiHKCI CYCGRG 1 cut(s) 764
BsiSI CCGG 3 cut(s) 89, 197, 240
BslFI GGGAC 4 cut(s) 129, 388, 476, 828
BslI CCNNNNNNNGG 6 cut(s) 69, 75, 110, 272, 670, 935
BsmAI GTCTC 2 cut(s) 314, 518
BsmFI GGGAC 4 cut(s) 129, 388, 476, 828
BsmI GAATGC 2 cut(s) 215, 574
BsnI GGCC 2 cut(s) 311, 458
BsoBI CYCGRG 1 cut(s) 764
Bsp1286I GDGCHC 2 cut(s) 38, 610
Bsp143I GATC 6 cut(s) 59, 100, 136, 628, 654, 967
BspACI CCGC 5 cut(s) 42, 76, 224, 229, 941
BspANI GGCC 2 cut(s) 311, 458
BspFNI CGCG 2 cut(s) 56, 58
BspLI GGNNCC 3 cut(s) 93, 492, 992
BspPI GGATC 5 cut(s) 54, 95, 131, 623, 962
BsrBI CCGCTC 1 cut(s) 229
BsrFI RCCGGY 2 cut(s) 88, 196
BsrI ACTGG 4 cut(s) 141, 382, 443, 625
BssAI RCCGGY 2 cut(s) 88, 196
BssECI CCNNGG 3 cut(s) 486, 961, 1087
BssHII GCGCGC 1 cut(s) 54
BssMI GATC 6 cut(s) 59, 100, 136, 628, 654, 967
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 961, 1087
Bst2UI CCWGG 2 cut(s) 70, 488
Bst4CI ACNGT 2 cut(s) 82, 179
BstACI GRCGYC 1 cut(s) 114
BstBAI YACGTR 1 cut(s) 1073
BstC8I GCNNGC 4 cut(s) 56, 309, 848, 903
BstDEI CTNAG 2 cut(s) 528, 842
BstEII GGTNACC 1 cut(s) 725
BstF5I GGATG 5 cut(s) 517, 775, 842, 1050, 1098
BstFNI CGCG 2 cut(s) 56, 58
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 56, 58
BstKTI GATC 6 cut(s) 62, 103, 139, 631, 657, 970
BstMAI GTCTC 2 cut(s) 314, 518
BstMBI GATC 6 cut(s) 59, 100, 136, 628, 654, 967
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 2 cut(s) 70, 488
BstNSI RCATGY 2 cut(s) 387, 606
BstPI GGTNACC 1 cut(s) 725
BstSCI CCNGG 2 cut(s) 68, 486
BstUI CGCG 2 cut(s) 56, 58
BstV1I GCAGC 3 cut(s) 365, 907, 910
BstX2I RGATCY 2 cut(s) 100, 654
BstXI CCANNNNNNTGG 1 cut(s) 1073
BstYI RGATCY 2 cut(s) 100, 654
BsuRI GGCC 2 cut(s) 311, 458
BtsCI GGATG 5 cut(s) 517, 775, 842, 1050, 1098
BtsI GCAGTG 2 cut(s) 283, 363
BtsIMutI CAGTG 4 cut(s) 283, 363, 450, 618
Cac8I GCNNGC 4 cut(s) 56, 309, 848, 903
CfoI GCGC 3 cut(s) 7, 56, 58
Cfr10I RCCGGY 2 cut(s) 88, 196
Cfr13I GGNCC 3 cut(s) 456, 490, 938
CseI GACGC 1 cut(s) 103
Csp6I GTAC 3 cut(s) 175, 661, 1070
CspCI CAANNNNNGTGG 2 cut(s) 607, 642
CviAII CATG 9 cut(s) 64, 253, 384, 453, 599, 603, 700, 913, 935
CviQI GTAC 3 cut(s) 175, 661, 1070
DdeI CTNAG 2 cut(s) 528, 842
DpnI GATC 6 cut(s) 61, 102, 138, 630, 656, 969
DpnII GATC 6 cut(s) 59, 100, 136, 628, 654, 967
DrdI GACNNNNNNGTC 1 cut(s) 822
DseDI GACNNNNNNGTC 1 cut(s) 822
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 961, 1087
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 490, 938
Eco53kI GAGCTC 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 764
Eco91I GGTNACC 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 36
EcoO109I RGGNCCY 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 68, 486
EcoT14I CCWWGG 2 cut(s) 961, 1087
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 38
ErhI CCWWGG 2 cut(s) 961, 1087
FaeI CATG 9 cut(s) 67, 256, 387, 456, 602, 606, 703, 916, 938
FaqI GGGAC 4 cut(s) 129, 388, 476, 828
FatI CATG 9 cut(s) 63, 252, 383, 452, 598, 602, 699, 912, 934
Fnu4HI GCNGC 3 cut(s) 354, 896, 899
FokI GGATG 4 cut(s) 504, 782, 849, 1057
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 3 cut(s) 354, 896, 899
FspBI CTAG 1 cut(s) 720
GlaI GCGC 3 cut(s) 6, 55, 57
GluI GCNGC 3 cut(s) 354, 896, 899
GsuI CTGGAG 2 cut(s) 496, 642
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 311, 458
HapII CCGG 3 cut(s) 89, 197, 240
HgaI GACGC 1 cut(s) 103
HhaI GCGC 3 cut(s) 7, 56, 58
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 9 cut(s) 67, 256, 387, 456, 602, 606, 703, 916, 938
Hin6I GCGC 3 cut(s) 5, 54, 56
HinP1I GCGC 3 cut(s) 5, 54, 56
HincII GTYRAC 1 cut(s) 954
HindII GTYRAC 1 cut(s) 954
HinfI GANTC 3 cut(s) 165, 425, 816
HpaII CCGG 3 cut(s) 89, 197, 240
HphI GGTGA 2 cut(s) 109, 977
Hpy166II GTNNAC 1 cut(s) 954
Hpy188I TCNGA 4 cut(s) 328, 637, 828, 995
Hpy188III TCNNGA 6 cut(s) 422, 475, 521, 754, 764, 971
Hpy8I GTNNAC 1 cut(s) 954
HpyAV CCTTC 4 cut(s) 136, 642, 643, 700
HpyCH4III ACNGT 2 cut(s) 82, 179
HpyCH4IV ACGT 2 cut(s) 412, 1072
HpyCH4V TGCA 7 cut(s) 361, 383, 545, 602, 743, 803, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 528, 842
HpySE526I ACGT 2 cut(s) 412, 1072
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 9 cut(s) 67, 256, 387, 456, 602, 606, 703, 916, 938
HspAI GCGC 3 cut(s) 5, 54, 56
Kzo9I GATC 6 cut(s) 59, 100, 136, 628, 654, 967
LmnI GCTCC 6 cut(s) 33, 41, 97, 226, 577, 996
Lsp1109I GCAGC 3 cut(s) 365, 907, 910
LweI GCATC 3 cut(s) 472, 730, 827
MaeI CTAG 1 cut(s) 720
MaeII ACGT 2 cut(s) 412, 1072
MaeIII GTNAC 3 cut(s) 115, 725, 920
MalI GATC 6 cut(s) 61, 102, 138, 630, 656, 969
MbiI CCGCTC 1 cut(s) 229
MboI GATC 6 cut(s) 59, 100, 136, 628, 654, 967
MboII GAAGA 5 cut(s) 254, 295, 305, 869, 991
MflI RGATCY 2 cut(s) 100, 654
MhlI GDGCHC 2 cut(s) 38, 610
MluCI AATT 5 cut(s) 245, 329, 780, 786, 982
MlyI GAGTC 2 cut(s) 174, 810
MmeI TCCRAC 2 cut(s) 109, 142
MnlI CCTC 5 cut(s) 140, 225, 504, 631, 760
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 1 cut(s) 261
MseI TTAA 1 cut(s) 1002
MslI CAYNNNNRTG 1 cut(s) 603
MspI CCGG 3 cut(s) 89, 197, 240
MspR9I CCNGG 2 cut(s) 70, 488
Mva1269I GAATGC 2 cut(s) 215, 574
MvaI CCWGG 2 cut(s) 70, 488
MvnI CGCG 2 cut(s) 56, 58
MwoI GCNNNNNNNGC 1 cut(s) 131
NdeII GATC 6 cut(s) 59, 100, 136, 628, 654, 967
NlaIII CATG 9 cut(s) 67, 256, 387, 456, 602, 606, 703, 916, 938
NlaIV GGNNCC 3 cut(s) 93, 492, 992
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 387, 606
PaeR7I CTCGAG 1 cut(s) 764
PauI GCGCGC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 2 cut(s) 215, 574
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 69
PkrI GCNGC 3 cut(s) 355, 897, 900
PleI GAGTC 2 cut(s) 173, 810
PpsI GAGTC 2 cut(s) 173, 810
Ppu21I YACGTR 1 cut(s) 1073
PpuMI RGGWCCY 1 cut(s) 490
Psp124BI GAGCTC 1 cut(s) 38
Psp1406I AACGTT 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 2 cut(s) 68, 486
PspEI GGTNACC 1 cut(s) 725
PspGI CCWGG 2 cut(s) 68, 486
PspN4I GGNNCC 3 cut(s) 93, 492, 992
PspPI GGNCC 3 cut(s) 456, 490, 938
PspPPI RGGWCCY 1 cut(s) 490
PsuI RGATCY 2 cut(s) 100, 654
PteI GCGCGC 1 cut(s) 54
RsaI GTAC 3 cut(s) 176, 662, 1071
RsaNI GTAC 3 cut(s) 175, 661, 1070
RseI CAYNNNNRTG 1 cut(s) 603
SacI GAGCTC 1 cut(s) 38
SaqAI TTAA 1 cut(s) 1002
SatI GCNGC 3 cut(s) 354, 896, 899
Sau3AI GATC 6 cut(s) 59, 100, 136, 628, 654, 967
Sau96I GGNCC 3 cut(s) 456, 490, 938
SchI GAGTC 2 cut(s) 174, 810
ScrFI CCNGG 2 cut(s) 70, 488
SduI GDGCHC 2 cut(s) 38, 610
SfaNI GCATC 3 cut(s) 472, 730, 827
Sfr274I CTCGAG 1 cut(s) 764
SinI GGWCC 2 cut(s) 490, 938
SlaI CTCGAG 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 5 cut(s) 245, 329, 780, 786, 982
SsiI CCGC 5 cut(s) 42, 76, 224, 229, 941
SspMI CTAG 1 cut(s) 720
SstI GAGCTC 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 68, 486
StyI CCWWGG 2 cut(s) 961, 1087
TaaI ACNGT 2 cut(s) 82, 179
TaiI ACGT 2 cut(s) 415, 1075
TaqI TCGA 1 cut(s) 765
TasI AATT 5 cut(s) 245, 329, 780, 786, 982
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 1 cut(s) 1002
Tru9I TTAA 1 cut(s) 1002
TscAI CASTG 4 cut(s) 283, 363, 450, 625
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 3 cut(s) 353, 895, 898
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 3 cut(s) 587, 876, 923
TspRI CASTG 4 cut(s) 283, 363, 450, 625
Van91I CCANNNNNTGG 1 cut(s) 69
VpaK11BI GGWCC 2 cut(s) 490, 938
XapI RAATTY 3 cut(s) 329, 786, 982
XceI RCATGY 2 cut(s) 387, 606
XcmI CCANNNNNNNNNTGG 2 cut(s) 46, 450
XhoI CTCGAG 1 cut(s) 764
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.