Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0010223

Physical Location & Seq

Genomic Coordinates Forward (+)

39775001 .. 39780725

Gene Structure 5725 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00113960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGGCGCTCTCCACCAAACTTTTTCAACGGAGGAGCTTCAGCTCCACCGCAGTCTTGGGCGCGCGATCCATGTCCTGGTGGAGAAGTGTCCAACCGGCTCCCAAAGATCCCATTCTCGGCGTCACCGAAGCCTTCCTCGCCGATCCCAGTCCCAACAAAGTCAATGTTGGAGTCGGTGCGTACCGTGATGACAATGGAAAGCCGGTTGTTCTGGAATGCGTTAGAGAAGCGGAGCGGAGGGTTGCCGGAAAATTAAACATGGAATATCTTCCTATGGGGGGCAGTGTGAAGATGGTGGAAGAAACATTGAAGCTGGCCTATGGAGAGACTTCTGAATTTCTCAAAGATAAAAGGATAGCAGCAGTGCAATCTCTTTCTGGGACTGGTGCATGTCGTCTTTTTGCAGACTTTCAAAAACGTTTTCGTCCAGATTCAAATATCTATATTCCAGTGCCAACATGGGCCAACCACCATAACATCTGGAGAGATGCCCAGGTCCCTCAAAGGACTTTCCATTACTATCATCCAGAGACTAAGGGTTTGGACTTTGCAGGACTGATTGATGACATAAAGAATGCTCCAAGTGGCTCATTCTTTCTACTTCATGCATGTGCTCACAATCCCACTGGAGTTGATCCTTCAGAGGAACAATGGAAAGAGATCTCATACCAAATCAAGGGTAAAGGTCATTTTGCCTTCTTTGACATGGCATATCAAGGTTTTGCTAGTGGTAACCCAGAGAGAGATGCAAAAGCCATCAGGATTTTTCTCGAGGATGGTCATCTAATTGGAATTTCTCAATCTTATGCAAAAAATATGGGACTCTATGGTCAGAGAGTAGGATGCCTTAGCTTGCTTTGTGAGGATGAAAAACAAGCTGTGGCTGTAAAAAGTCAGTTGCAGCAGCTTGCCAGACCCATGTATAGTAACCCACCCGTTCATGGACCGCTTATAGTGTCAACTATCCTTGGTGATCCAGACTTGAAGAATTTGTGGCTCCGAGAAGTTAAGGGCATGGCAGATCGCATAATCGGAATGCGAACTGCTCTACGAGAAAACCTTGAGAAATTGGGATCACCCTTATCTTGGGAGCACATAACAAATCAGATTGGCATGTTTTGCTATAGCGGAATGACACCTGAACAAGTTGATCGTTTGACAAATGAGTTTCATATATATTTGACTCGCAATGGTCGAATAAGTATGGCGGGTGTTACAACGGGCAATGTTGGATATCTGGCAAATGCTATACATGAAGTAACAAAATCTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004069 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006103 GO:0006520 GO:0006531 GO:0006536 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009064 GO:0009066 GO:0009987 GO:0010035 GO:0010038 GO:0016740 GO:0016769 GO:0019752 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0051186 GO:0071704 GO:1901564 GO:1901605

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00270 ko00330 ko00350 ko00360 ko00400 ko00710 ko00950 ko00960 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04975 map00220 map00250 map00270 map00330 map00350 map00360 map00400 map00710 map00950 map00960 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04975

Pfam Domains

Protein Families

Aminotran_1_2

Protein Analysis

424

Amino Acids

47.24

Weight (kDa)

8.59

Isoelectric Point (pI)

42.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aminotran_1_2	PF00155	52 - 417	9.2e-91	Aminotransferase class I and II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010223)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30970 AT2G30970
fragaria_vesca	FvH4_6g27650 FvH4_6g27650 FvH4_6g27650 FvH4_6g27650
malus_domestica	MD17G1264900.v1.1
prunus_persica	Prupe.3G115000_v2.0.a1 Prupe.3G115000_v2.0.a1 Prupe.3G115000_v2.0.a1
pyrus_communis	pycom17g26460
rosa_chinensis	RchiOBHm_Chr2g0130551
rosa_laevigata	RLG00000019136
rosa_multiflora	Rmu_sc0001336.1_g000028
rosa_roxburghii	Rroxscaffold_2G00113960
rosa_rugosa	Rorug02G0292000
rosa_samantha	Rh2AG343500 Rh2BG352000 Rh2CG330700 Rh2DG370000
rosa_wichuraiana	Rw2G027570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	828
AccB7I	CCANNNNNTGG	1 cut(s)	75
AccBSI	CCGCTC	1 cut(s)	235
AccII	CGCG	2 cut(s)	62, 64
AciI	CCGC	6 cut(s)	48, 230, 235, 947, 1128, 1208
AclI	AACGTT	1 cut(s)	418
AclWI	GGATC	6 cut(s)	60, 101, 137, 629, 968, 1081
AcsI	RAATTY	3 cut(s)	335, 792, 988
AcuI	CTGAAG	2 cut(s)	22, 624
AcyI	GRCGYC	1 cut(s)	120
AfaI	GTAC	1 cut(s)	182
AfiI	CCNNNNNNNGG	6 cut(s)	75, 116, 278, 676, 941, 1086
AgsI	TTSAA	5 cut(s)	26, 310, 413, 435, 985
AjnI	CCWGG	2 cut(s)	74, 492
AluBI	AGCT	6 cut(s)	36, 42, 313, 852, 878, 907
AluI	AGCT	6 cut(s)	36, 42, 313, 852, 878, 907
Alw21I	GWGCWC	2 cut(s)	616, 1095
Alw26I	GTCTC	2 cut(s)	320, 524
AlwI	GGATC	6 cut(s)	60, 101, 137, 629, 968, 1081
Ama87I	CYCGRG	1 cut(s)	770
AoxI	GGCC	2 cut(s)	315, 462
ApeKI	GCWGC	3 cut(s)	359, 901, 904
ApoI	RAATTY	3 cut(s)	335, 792, 988
ArsI	GACNNNNNNTTYG	2 cut(s)	523, 555
Asp700I	GAANNNNTTC	1 cut(s)	267
AspLEI	GCGC	3 cut(s)	7, 62, 64
AspS9I	GGNCC	3 cut(s)	462, 496, 944
AsuHPI	GGTGA	3 cut(s)	115, 983, 1068
AvaI	CYCGRG	1 cut(s)	770
AvaII	GGWCC	2 cut(s)	496, 944
Bbv12I	GWGCWC	2 cut(s)	616, 1095
BbvI	GCAGC	3 cut(s)	371, 913, 916
BccI	CCATC	3 cut(s)	286, 764, 770
BciT130I	CCWGG	2 cut(s)	76, 494
BcoDI	GTCTC	2 cut(s)	320, 524
BfaI	CTAG	1 cut(s)	726
BfmI	CTRYAG	1 cut(s)	1123
BfoI	RGCGCY	1 cut(s)	8
BglII	AGATCT	1 cut(s)	660
BisI	GCNGC	3 cut(s)	360, 902, 905
BlsI	GCNGC	3 cut(s)	361, 903, 906
Bme1390I	CCNGG	2 cut(s)	76, 494
Bme18I	GGWCC	2 cut(s)	496, 944
BmeT110I	CYCGRG	1 cut(s)	770
BmgT120I	GGNCC	3 cut(s)	462, 496, 944
BmiI	GGNNCC	3 cut(s)	99, 498, 998
BmrFI	CCNGG	2 cut(s)	76, 494
BmrI	ACTGGG	1 cut(s)	141
BmsI	GCATC	3 cut(s)	478, 736, 833
BmuI	ACTGGG	1 cut(s)	141
BpmI	CTGGAG	2 cut(s)	502, 648
Bpu10I	CCTNAGC	1 cut(s)	848
BpuEI	CTTGAG	1 cut(s)	1082
BsaBI	GATNNNNATC	1 cut(s)	780
BsaHI	GRCGYC	1 cut(s)	120
BsaJI	CCNNGG	2 cut(s)	492, 967
Bsc4I	CCNNNNNNNGG	6 cut(s)	75, 116, 278, 676, 941, 1086
Bse118I	RCCGGY	2 cut(s)	94, 202
Bse1I	ACTGG	4 cut(s)	147, 388, 449, 631
Bse3DI	GCAATG	2 cut(s)	1195, 1231
Bse8I	GATNNNNATC	1 cut(s)	780
BseBI	CCWGG	2 cut(s)	76, 494
BseDI	CCNNGG	2 cut(s)	492, 967
BseGI	GGATG	4 cut(s)	523, 781, 848, 871
BseJI	GATNNNNATC	1 cut(s)	780
BseLI	CCNNNNNNNGG	6 cut(s)	75, 116, 278, 676, 941, 1086
BseMI	GCAATG	2 cut(s)	1195, 1231
BseNI	ACTGG	4 cut(s)	147, 388, 449, 631
BsePI	GCGCGC	1 cut(s)	60
BseRI	GAGGAG	1 cut(s)	46
BseXI	GCAGC	3 cut(s)	371, 913, 916
Bsh1236I	CGCG	2 cut(s)	62, 64
BshFI	GGCC	2 cut(s)	317, 464
BsiHKAI	GWGCWC	2 cut(s)	616, 1095
BsiHKCI	CYCGRG	1 cut(s)	770
BsiSI	CCGG	3 cut(s)	95, 203, 246
BslFI	GGGAC	4 cut(s)	135, 394, 482, 834
BslI	CCNNNNNNNGG	6 cut(s)	75, 116, 278, 676, 941, 1086
BsmAI	GTCTC	2 cut(s)	320, 524
BsmFI	GGGAC	4 cut(s)	135, 394, 482, 834
BsmI	GAATGC	3 cut(s)	221, 580, 1041
BsnI	GGCC	2 cut(s)	317, 464
BsoBI	CYCGRG	1 cut(s)	770
Bsp1286I	GDGCHC	2 cut(s)	616, 1095
Bsp143I	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
BspACI	CCGC	6 cut(s)	48, 230, 235, 947, 1128, 1208
BspANI	GGCC	2 cut(s)	317, 464
BspFNI	CGCG	2 cut(s)	62, 64
BspLI	GGNNCC	3 cut(s)	99, 498, 998
BspPI	GGATC	6 cut(s)	60, 101, 137, 629, 968, 1081
BsrBI	CCGCTC	1 cut(s)	235
BsrDI	GCAATG	2 cut(s)	1195, 1231
BsrFI	RCCGGY	2 cut(s)	94, 202
BsrI	ACTGG	4 cut(s)	147, 388, 449, 631
BssAI	RCCGGY	2 cut(s)	94, 202
BssECI	CCNNGG	2 cut(s)	492, 967
BssHII	GCGCGC	1 cut(s)	60
BssMI	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
BssNI	GRCGYC	1 cut(s)	120
BssT1I	CCWWGG	1 cut(s)	967
Bst2UI	CCWGG	2 cut(s)	76, 494
Bst4CI	ACNGT	1 cut(s)	185
BstACI	GRCGYC	1 cut(s)	120
BstAPI	GCANNNNNTGC	1 cut(s)	1119
BstC8I	GCNNGC	4 cut(s)	62, 315, 854, 909
BstDEI	CTNAG	2 cut(s)	534, 848
BstEII	GGTNACC	1 cut(s)	731
BstF5I	GGATG	4 cut(s)	523, 781, 848, 871
BstFNI	CGCG	2 cut(s)	62, 64
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	3 cut(s)	7, 62, 64
BstKTI	GATC	9 cut(s)	68, 109, 145, 637, 663, 976, 1024, 1076, 1153
BstMAI	GTCTC	2 cut(s)	320, 524
BstMBI	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
BstMWI	GCNNNNNNNGC	2 cut(s)	137, 1119
BstNI	CCWGG	2 cut(s)	76, 494
BstNSI	RCATGY	3 cut(s)	393, 612, 1117
BstPI	GGTNACC	1 cut(s)	731
BstSCI	CCNGG	2 cut(s)	74, 492
BstSFI	CTRYAG	1 cut(s)	1123
BstUI	CGCG	2 cut(s)	62, 64
BstV1I	GCAGC	3 cut(s)	371, 913, 916
BstX2I	RGATCY	2 cut(s)	106, 660
BstYI	RGATCY	2 cut(s)	106, 660
BsuRI	GGCC	2 cut(s)	317, 464
BtsCI	GGATG	4 cut(s)	523, 781, 848, 871
BtsI	GCAGTG	2 cut(s)	289, 369
BtsIMutI	CAGTG	4 cut(s)	289, 369, 456, 624
Cac8I	GCNNGC	4 cut(s)	62, 315, 854, 909
CfoI	GCGC	3 cut(s)	7, 62, 64
Cfr10I	RCCGGY	2 cut(s)	94, 202
Cfr13I	GGNCC	3 cut(s)	462, 496, 944
CseI	GACGC	1 cut(s)	109
Csp6I	GTAC	1 cut(s)	181
CspCI	CAANNNNNGTGG	2 cut(s)	613, 648
CviQI	GTAC	1 cut(s)	181
DdeI	CTNAG	2 cut(s)	534, 848
DpnI	GATC	9 cut(s)	67, 108, 144, 636, 662, 975, 1023, 1075, 1152
DpnII	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
DrdI	GACNNNNNNGTC	1 cut(s)	828
DseDI	GACNNNNNNGTC	1 cut(s)	828
Eco130I	CCWWGG	1 cut(s)	967
Eco32I	GATATC	1 cut(s)	1235
Eco47I	GGWCC	2 cut(s)	496, 944
Eco57I	CTGAAG	2 cut(s)	22, 624
Eco88I	CYCGRG	1 cut(s)	770
Eco91I	GGTNACC	1 cut(s)	731
EcoO109I	RGGNCCY	1 cut(s)	496
EcoO65I	GGTNACC	1 cut(s)	731
EcoRII	CCWGG	2 cut(s)	74, 492
EcoRV	GATATC	1 cut(s)	1235
EcoT14I	CCWWGG	1 cut(s)	967
EcoT22I	ATGCAT	1 cut(s)	610
ErhI	CCWWGG	1 cut(s)	967
FaqI	GGGAC	4 cut(s)	135, 394, 482, 834
FauI	CCCGC	1 cut(s)	1201
Fnu4HI	GCNGC	3 cut(s)	360, 902, 905
FokI	GGATG	4 cut(s)	510, 788, 855, 878
Fsp4HI	GCNGC	3 cut(s)	360, 902, 905
FspBI	CTAG	1 cut(s)	726
GlaI	GCGC	3 cut(s)	6, 61, 63
GluI	GCNGC	3 cut(s)	360, 902, 905
GsuI	CTGGAG	2 cut(s)	502, 648
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	2 cut(s)	317, 464
HapII	CCGG	3 cut(s)	95, 203, 246
HgaI	GACGC	1 cut(s)	109
HhaI	GCGC	3 cut(s)	7, 62, 64
Hin1I	GRCGYC	1 cut(s)	120
Hin6I	GCGC	3 cut(s)	5, 60, 62
HinP1I	GCGC	3 cut(s)	5, 60, 62
HincII	GTYRAC	1 cut(s)	960
HindII	GTYRAC	1 cut(s)	960
HinfI	GANTC	4 cut(s)	171, 431, 822, 1183
HpaII	CCGG	3 cut(s)	95, 203, 246
HphI	GGTGA	3 cut(s)	115, 983, 1068
Hpy166II	GTNNAC	1 cut(s)	960
Hpy188I	TCNGA	6 cut(s)	334, 643, 834, 1001, 1034, 1107
Hpy188III	TCNNGA	7 cut(s)	212, 428, 481, 527, 760, 770, 977
Hpy8I	GTNNAC	1 cut(s)	960
HpyAV	CCTTC	3 cut(s)	142, 648, 706
HpyCH4III	ACNGT	1 cut(s)	185
HpyCH4IV	ACGT	1 cut(s)	418
HpyCH4V	TGCA	9 cut(s)	367, 389, 404, 551, 608, 749, 809, 901, 1271
HpyF10VI	GCNNNNNNNGC	2 cut(s)	137, 1119
HpyF3I	CTNAG	2 cut(s)	534, 848
HpySE526I	ACGT	1 cut(s)	418
Hsp92I	GRCGYC	1 cut(s)	120
HspAI	GCGC	3 cut(s)	5, 60, 62
Kzo9I	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
LmnI	GCTCC	7 cut(s)	33, 47, 103, 232, 583, 1002, 1090
Lsp1109I	GCAGC	3 cut(s)	371, 913, 916
LweI	GCATC	3 cut(s)	478, 736, 833
MaeI	CTAG	1 cut(s)	726
MaeII	ACGT	1 cut(s)	418
MaeIII	GTNAC	5 cut(s)	121, 731, 926, 1213, 1258
MalI	GATC	9 cut(s)	67, 108, 144, 636, 662, 975, 1023, 1075, 1152
MbiI	CCGCTC	1 cut(s)	235
MboI	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
MboII	GAAGA	4 cut(s)	260, 301, 311, 997
MflI	RGATCY	2 cut(s)	106, 660
MhlI	GDGCHC	2 cut(s)	616, 1095
MluCI	AATT	6 cut(s)	251, 335, 786, 792, 988, 1067
MlyI	GAGTC	3 cut(s)	180, 816, 1177
MmeI	TCCRAC	3 cut(s)	115, 148, 1210
MnlI	CCTC	7 cut(s)	24, 146, 231, 510, 637, 766, 856
Mph1103I	ATGCAT	1 cut(s)	610
MroXI	GAANNNNTTC	1 cut(s)	267
MseI	TTAA	2 cut(s)	254, 1008
MslI	CAYNNNNRTG	1 cut(s)	609
MspI	CCGG	3 cut(s)	95, 203, 246
MspR9I	CCNGG	2 cut(s)	76, 494
Mva1269I	GAATGC	3 cut(s)	221, 580, 1041
MvaI	CCWGG	2 cut(s)	76, 494
MvnI	CGCG	2 cut(s)	62, 64
MwoI	GCNNNNNNNGC	2 cut(s)	137, 1119
NdeII	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
NlaIV	GGNNCC	3 cut(s)	99, 498, 998
NmeAIII	GCCGAG	1 cut(s)	96
NmuCI	GTSAC	1 cut(s)	121
NsiI	ATGCAT	1 cut(s)	610
NspI	RCATGY	3 cut(s)	393, 612, 1117
PaeR7I	CTCGAG	1 cut(s)	770
PauI	GCGCGC	1 cut(s)	60
PcsI	WCGNNNNNNNCGW	2 cut(s)	123, 1192
PctI	GAATGC	3 cut(s)	221, 580, 1041
PdmI	GAANNNNTTC	1 cut(s)	267
PfeI	GAWTC	1 cut(s)	431
PflMI	CCANNNNNTGG	1 cut(s)	75
PkrI	GCNGC	3 cut(s)	361, 903, 906
PleI	GAGTC	3 cut(s)	179, 816, 1177
PpsI	GAGTC	3 cut(s)	179, 816, 1177
PpuMI	RGGWCCY	1 cut(s)	496
Psp1406I	AACGTT	1 cut(s)	418
Psp5II	RGGWCCY	1 cut(s)	496
Psp6I	CCWGG	2 cut(s)	74, 492
PspEI	GGTNACC	1 cut(s)	731
PspGI	CCWGG	2 cut(s)	74, 492
PspN4I	GGNNCC	3 cut(s)	99, 498, 998
PspPI	GGNCC	3 cut(s)	462, 496, 944
PspPPI	RGGWCCY	1 cut(s)	496
PsuI	RGATCY	2 cut(s)	106, 660
PteI	GCGCGC	1 cut(s)	60
RsaI	GTAC	1 cut(s)	182
RsaNI	GTAC	1 cut(s)	181
RseI	CAYNNNNRTG	1 cut(s)	609
SaqAI	TTAA	2 cut(s)	254, 1008
SatI	GCNGC	3 cut(s)	360, 902, 905
Sau3AI	GATC	9 cut(s)	65, 106, 142, 634, 660, 973, 1021, 1073, 1150
Sau96I	GGNCC	3 cut(s)	462, 496, 944
SchI	GAGTC	3 cut(s)	180, 816, 1177
ScrFI	CCNGG	2 cut(s)	76, 494
SduI	GDGCHC	2 cut(s)	616, 1095
SfaNI	GCATC	3 cut(s)	478, 736, 833
SfcI	CTRYAG	1 cut(s)	1123
Sfr274I	CTCGAG	1 cut(s)	770
SinI	GGWCC	2 cut(s)	496, 944
SlaI	CTCGAG	1 cut(s)	770
SmiMI	CAYNNNNRTG	1 cut(s)	609
SmlI	CTYRAG	2 cut(s)	770, 1061
SmoI	CTYRAG	2 cut(s)	770, 1061
Sse9I	AATT	6 cut(s)	251, 335, 786, 792, 988, 1067
SsiI	CCGC	6 cut(s)	48, 230, 235, 947, 1128, 1208
SspMI	CTAG	1 cut(s)	726
StyD4I	CCNGG	2 cut(s)	74, 492
StyI	CCWWGG	1 cut(s)	967
TaaI	ACNGT	1 cut(s)	185
TaiI	ACGT	1 cut(s)	421
TaqI	TCGA	2 cut(s)	771, 1195
TasI	AATT	6 cut(s)	251, 335, 786, 792, 988, 1067
TfiI	GAWTC	1 cut(s)	431
Tru1I	TTAA	2 cut(s)	254, 1008
Tru9I	TTAA	2 cut(s)	254, 1008
TscAI	CASTG	4 cut(s)	289, 369, 456, 631
TseFI	GTSAC	1 cut(s)	121
TseI	GCWGC	3 cut(s)	359, 901, 904
Tsp45I	GTSAC	1 cut(s)	121
TspDTI	ATGAA	5 cut(s)	593, 882, 929, 1160, 1269
TspGWI	ACGGA	1 cut(s)	43
TspRI	CASTG	4 cut(s)	289, 369, 456, 631
Van91I	CCANNNNNTGG	1 cut(s)	75
VpaK11BI	GGWCC	2 cut(s)	496, 944
XapI	RAATTY	3 cut(s)	335, 792, 988
XceI	RCATGY	3 cut(s)	393, 612, 1117
XcmI	CCANNNNNNNNNTGG	2 cut(s)	52, 456
XhoI	CTCGAG	1 cut(s)	770
XmnI	GAANNNNTTC	1 cut(s)	267
XspI	CTAG	1 cut(s)	726
Zsp2I	ATGCAT	1 cut(s)	610

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.