RchiOBHm_Chr2g0133701

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008460
Physical Location & Seq
Reverse (-)
50583362 .. 50585896
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50484

Sequence Viewer

Length: 1404 bp
ATGGATTATTTACCCGAGCAGTTAGTTTGGGAGATCTTGAGCAGGATTAAGAAAACCACTGATAGAAACTCCGTGTCTTTGACATGTAAACGCCTGAATGGATTGGATAATGAACAGAGGCAAGCCCTTCGGGTTGGTTGTGGATTAGACCCTGCTAATGAAGCTTTGACTTCTCTCTGCAGTAGGTTTCTCAACTTGACAAAGGTAGAGATAACTTACGCCGGTTGGATGTCCAAACTAGGAAAGCAGTTGGATGACCATGGGCTTCTTGTTCTTTCCAATTACTGCCCTTCTTTGGTTGATCTCTCACTAAGCTACTGCACATTCATCACTGATGTGGGCCTTGGTTATTTGTCTTCTTGCTCAAAGCTTTCAGCTTTGAAGTTGAATTTCACCCCAAGAATAACTGGCTGTGGCATATTATCTCTTGTTGTGGGCTGCAAAAATCTCACTGTTCTCCATCTCATCCGATGTTTAAATGTTAGCAGCGTCGAGTGGCTTGAATATCTTGGCAGGCTTGAGACACTTGAAGATCTCTCAATCAAGAATTGTAGAGCTATTGGGGAGGGCGATATGATTAAGCTAGGTGCTAGTTGGAGAAAACTGAAACGCTTGCAATTTGAGGTGGATGCTAATTACAGATACATGAAAGTCTATGATCGTTTAGCTGTGGATCGATGGCAAAAGCAGTGGGTTCCTTGTGAGAGTATGTTGGAACTTAGCTTGATAAATTGCATCATCAGCCCAGGAAGGGGACTTGCTTGTGTGATGGGGAAGTGCAAGAAATTGGAGAAGATTCACTTGGACATGTGTGTTGGGGTACGGGACTGTGACATAATAGGCTTAGCCCAAAATTCAAGTAATCTTCGCTCCATTTACCTCCGAGTCCCGTCAGATTTCTCACTTCCTCTTCTGATGAATAACCCTTTGAGACTAACCGATGAATGCCTAAATGCCTTGTCCAGTAACTGTTCAATGCTTGAATCTGTCAGGATATCTTACTCTGATGGGGAATTCCCTTCCTTTTCCTCATTTACATTACAGGGAATCATTACTTTGATTCAGAAGTGCCCTGTGCGGGAACTTGCTTTTGACCAAGTGTATTCCTTCAACGACACTGGGATGGAAGCTCTTTGCTTTGCTCAGCATCTTGAAACATTGGAGCTTGTCAGGTGCCAAGAGATAAGTGATGAAGGGCTGCAACTTGCGGTCCAGTTTCCGCGATTGTCTGTTCTGCGTTTAATCAAGTGTTTGGGAGTTTCTGATGAAGGTTTAAGGCCACTAGTAGGTTCATTCAAACTGGAAACGTTGGCTGTGGAAGATTGTCCCCAAGTCTCTGAAAGAGGAGTATTTGGAGCTGCAAGATCTGTTTCTTTCAGACAAGACCTGTCATGGATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
F-box LRR_6

Protein Analysis

467

Amino Acids

52.44

Weight (kDa)

6.48

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 377 - 450 2.4e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1173
AccII CGCG 1 cut(s) 1222
AciI CCGC 3 cut(s) 1078, 1208, 1220
AclI AACGTT 1 cut(s) 1307
AclWI GGATC 1 cut(s) 681
AcsI RAATTY 3 cut(s) 388, 853, 1013
AfaI GTAC 2 cut(s) 822, 1400
AfiI CCNNNNNNNGG 5 cut(s) 295, 751, 752, 1078, 1286
AflIII ACRYGT 2 cut(s) 83, 807
AhlI ACTAGT 1 cut(s) 1282
AjnI CCWGG 1 cut(s) 745
AjuI GAANNNNNNNTTGG 2 cut(s) 785, 817
AleI CACNNNNGTG 1 cut(s) 335
Alw26I GTCTC 3 cut(s) 515, 925, 1339
AlwI GGATC 1 cut(s) 681
AlwNI CAGNNNCTG 1 cut(s) 969
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 2 cut(s) 340, 1277
ApeKI GCWGC 4 cut(s) 438, 486, 1198, 1358
ApoI RAATTY 3 cut(s) 388, 853, 1013
AspS9I GGNCC 2 cut(s) 340, 1210
AsuHPI GGTGA 1 cut(s) 385
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 1210
BaeGI GKGCMC 1 cut(s) 1073
BanI GGYRCC 1 cut(s) 1173
BbsI GAAGAC 1 cut(s) 348
BbvI GCAGC 4 cut(s) 425, 498, 1185, 1345
BccI CCATC 5 cut(s) 468, 672, 763, 1001, 1117
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BciT130I CCWGG 1 cut(s) 747
BcoDI GTCTC 3 cut(s) 515, 925, 1339
BcuI ACTAGT 1 cut(s) 1282
BfaI CTAG 4 cut(s) 239, 584, 591, 1283
BfmI CTRYAG 1 cut(s) 178
BglII AGATCT 3 cut(s) 33, 532, 1364
BisI GCNGC 4 cut(s) 439, 487, 1199, 1359
BlpI GCTNAGC 2 cut(s) 844, 1143
BlsI GCNGC 4 cut(s) 440, 488, 1200, 1360
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 1210
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 340, 1210
BmiI GGNNCC 2 cut(s) 696, 1175
BmrFI CCNGG 1 cut(s) 747
BmrI ACTGGG 1 cut(s) 1128
BmsI GCATC 3 cut(s) 619, 744, 1156
BmuI ACTGGG 1 cut(s) 1128
BpiI GAAGAC 1 cut(s) 348
Bpu1102I GCTNAGC 2 cut(s) 844, 1143
BpuEI CTTGAG 2 cut(s) 58, 539
Bsa29I ATCGAT 1 cut(s) 676
BsaJI CCNNGG 3 cut(s) 259, 343, 745
Bsc4I CCNNNNNNNGG 5 cut(s) 295, 751, 752, 1078, 1286
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 5 cut(s) 412, 963, 1123, 1213, 1305
BseBI CCWGG 1 cut(s) 747
BseCI ATCGAT 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 259, 343, 745
BseGI GGATG 6 cut(s) 234, 259, 465, 634, 1128, 1401
BseLI CCNNNNNNNGG 5 cut(s) 295, 751, 752, 1078, 1286
BseMII CTCAG 1 cut(s) 1157
BseNI ACTGG 5 cut(s) 412, 963, 1123, 1213, 1305
BseRI GAGGAG 1 cut(s) 1359
BseSI GKGCMC 1 cut(s) 1073
BseXI GCAGC 4 cut(s) 425, 498, 1185, 1345
BsgI GTGCAG 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 1222
BshFI GGCC 2 cut(s) 342, 1279
BshNI GGYRCC 1 cut(s) 1173
BshVI ATCGAT 1 cut(s) 676
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 4 cut(s) 768, 839, 872, 1311
BslI CCNNNNNNNGG 5 cut(s) 295, 751, 752, 1078, 1286
BsmAI GTCTC 3 cut(s) 515, 925, 1339
BsmFI GGGAC 4 cut(s) 768, 839, 872, 1311
BsmI GAATGC 1 cut(s) 950
BsnI GGCC 2 cut(s) 342, 1279
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 1073
Bsp143I GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
Bsp1720I GCTNAGC 2 cut(s) 844, 1143
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 3 cut(s) 1078, 1208, 1220
BspANI GGCC 2 cut(s) 342, 1279
BspCNI CTCAG 1 cut(s) 1156
BspDI ATCGAT 1 cut(s) 676
BspFNI CGCG 1 cut(s) 1222
BspLI GGNNCC 2 cut(s) 696, 1175
BspMAI CTGCAG 1 cut(s) 182
BspPI GGATC 1 cut(s) 681
BspT107I GGYRCC 1 cut(s) 1173
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 5 cut(s) 412, 963, 1123, 1213, 1305
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 259, 343, 745
BssMI GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
BssT1I CCWWGG 2 cut(s) 259, 343
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 3 cut(s) 454, 830, 971
Bst6I CTCTTC 1 cut(s) 915
BstC8I GCNNGC 3 cut(s) 123, 515, 614
BstDEI CTNAG 4 cut(s) 311, 719, 844, 1143
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 6 cut(s) 234, 259, 465, 634, 1128, 1401
BstFNI CGCG 1 cut(s) 1222
BstKTI GATC 6 cut(s) 36, 304, 535, 661, 676, 1367
BstMAI GTCTC 3 cut(s) 515, 925, 1339
BstMBI GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
BstMWI GCNNNNNNNGC 2 cut(s) 161, 741
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 2 cut(s) 87, 811
BstSCI CCNGG 1 cut(s) 745
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 1073
BstUI CGCG 1 cut(s) 1222
BstV1I GCAGC 4 cut(s) 425, 498, 1185, 1345
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 3 cut(s) 33, 532, 1364
BstYI RGATCY 3 cut(s) 33, 532, 1364
Bsu15I ATCGAT 1 cut(s) 676
BsuRI GGCC 2 cut(s) 342, 1279
BsuTUI ATCGAT 1 cut(s) 676
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 6 cut(s) 234, 259, 465, 634, 1128, 1401
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 5 cut(s) 57, 330, 450, 695, 1116
Cac8I GCNNGC 3 cut(s) 123, 515, 614
CaiI CAGNNNCTG 1 cut(s) 969
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 340, 1210
ClaI ATCGAT 1 cut(s) 676
CseI GACGC 1 cut(s) 478
Csp6I GTAC 2 cut(s) 821, 1399
CspCI CAANNNNNGTGG 2 cut(s) 671, 706
CviAII CATG 5 cut(s) 84, 260, 646, 808, 1392
CviQI GTAC 2 cut(s) 821, 1399
DdeI CTNAG 4 cut(s) 311, 719, 844, 1143
DpnI GATC 6 cut(s) 35, 303, 534, 660, 675, 1366
DpnII GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
DraI TTTAAA 1 cut(s) 477
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Eco130I CCWWGG 2 cut(s) 259, 343
Eco32I GATATC 1 cut(s) 996
Eco47I GGWCC 1 cut(s) 1210
Eco88I CYCGRG 1 cut(s) 14
EcoRI GAATTC 1 cut(s) 1013
EcoRII CCWGG 1 cut(s) 745
EcoRV GATATC 1 cut(s) 996
EcoT14I CCWWGG 2 cut(s) 259, 343
ErhI CCWWGG 2 cut(s) 259, 343
FaeI CATG 5 cut(s) 87, 263, 649, 811, 1395
FalI AAGNNNNNCTT 2 cut(s) 785, 817
FaqI GGGAC 4 cut(s) 768, 839, 872, 1311
FatI CATG 5 cut(s) 83, 259, 645, 807, 1391
FauI CCCGC 1 cut(s) 1071
Fnu4HI GCNGC 4 cut(s) 439, 487, 1199, 1359
FokI GGATG 5 cut(s) 241, 266, 452, 641, 1135
Fsp4HI GCNGC 4 cut(s) 439, 487, 1199, 1359
FspBI CTAG 4 cut(s) 239, 584, 591, 1283
GluI GCNGC 4 cut(s) 439, 487, 1199, 1359
HaeIII GGCC 2 cut(s) 342, 1279
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 478
Hin1II CATG 5 cut(s) 87, 263, 649, 811, 1395
HindIII AAGCTT 2 cut(s) 162, 368
HinfI GANTC 5 cut(s) 796, 885, 983, 1047, 1060
HpaII CCGG 1 cut(s) 222
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 9 cut(s) 470, 884, 895, 915, 1006, 1065, 1264, 1339, 1379
Hpy188III TCNNGA 4 cut(s) 37, 544, 991, 1151
Hpy8I GTNNAC 1 cut(s) 89
Hpy99I CGWCG 1 cut(s) 494
HpyAV CCTTC 7 cut(s) 137, 300, 744, 1029, 1117, 1187, 1262
HpyCH4III ACNGT 3 cut(s) 454, 830, 971
HpyCH4IV ACGT 1 cut(s) 1307
HpyCH4V TGCA 8 cut(s) 180, 321, 441, 616, 735, 780, 1201, 1361
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 741
HpyF3I CTNAG 4 cut(s) 311, 719, 844, 1143
HpySE526I ACGT 1 cut(s) 1307
Hsp92II CATG 5 cut(s) 87, 263, 649, 811, 1395
Kzo9I GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
LmnI GCTCC 3 cut(s) 875, 1162, 1355
Lsp1109I GCAGC 4 cut(s) 425, 498, 1185, 1345
LweI GCATC 3 cut(s) 619, 744, 1156
MaeI CTAG 4 cut(s) 239, 584, 591, 1283
MaeII ACGT 1 cut(s) 1307
MaeIII GTNAC 2 cut(s) 830, 965
MalI GATC 6 cut(s) 35, 303, 534, 660, 675, 1366
MboI GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
MboII GAAGA 6 cut(s) 348, 542, 805, 857, 902, 1331
MflI RGATCY 3 cut(s) 33, 532, 1364
MhlI GDGCHC 1 cut(s) 1073
MluCI AATT 9 cut(s) 280, 388, 547, 617, 634, 730, 785, 853, 1013
MlyI GAGTC 1 cut(s) 894
MmeI TCCRAC 4 cut(s) 206, 231, 575, 693
MnlI CCTC 7 cut(s) 111, 559, 616, 890, 918, 1039, 1337
MseI TTAA 5 cut(s) 48, 476, 579, 1241, 1274
MslI CAYNNNNRTG 2 cut(s) 335, 1121
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 950
MvaI CCWGG 1 cut(s) 747
MvnI CGCG 1 cut(s) 1222
MwoI GCNNNNNNNGC 2 cut(s) 161, 741
NcoI CCATGG 1 cut(s) 259
NdeII GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
NlaIII CATG 5 cut(s) 87, 263, 649, 811, 1395
NlaIV GGNNCC 2 cut(s) 696, 1175
NmuCI GTSAC 1 cut(s) 830
NspI RCATGY 2 cut(s) 87, 811
OliI CACNNNNGTG 1 cut(s) 335
PciI ACATGT 2 cut(s) 83, 807
PctI GAATGC 1 cut(s) 950
PfeI GAWTC 4 cut(s) 796, 983, 1047, 1060
PkrI GCNGC 4 cut(s) 440, 488, 1200, 1360
PleI GAGTC 1 cut(s) 893
PpsI GAGTC 1 cut(s) 893
PscI ACATGT 2 cut(s) 83, 807
Psp1406I AACGTT 1 cut(s) 1307
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 2 cut(s) 696, 1175
PspPI GGNCC 2 cut(s) 340, 1210
PstI CTGCAG 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 969
PsuI RGATCY 3 cut(s) 33, 532, 1364
RsaI GTAC 2 cut(s) 822, 1400
RsaNI GTAC 2 cut(s) 821, 1399
RseI CAYNNNNRTG 2 cut(s) 335, 1121
SaqAI TTAA 5 cut(s) 48, 476, 579, 1241, 1274
SatI GCNGC 4 cut(s) 439, 487, 1199, 1359
Sau3AI GATC 6 cut(s) 33, 301, 532, 658, 673, 1364
Sau96I GGNCC 2 cut(s) 340, 1210
SchI GAGTC 1 cut(s) 894
ScrFI CCNGG 1 cut(s) 747
SduI GDGCHC 1 cut(s) 1073
SfaNI GCATC 3 cut(s) 619, 744, 1156
SfcI CTRYAG 1 cut(s) 178
SinI GGWCC 1 cut(s) 1210
SmiMI CAYNNNNRTG 2 cut(s) 335, 1121
SmlI CTYRAG 2 cut(s) 37, 518
SmoI CTYRAG 2 cut(s) 37, 518
SpeI ACTAGT 1 cut(s) 1282
Sse9I AATT 9 cut(s) 280, 388, 547, 617, 634, 730, 785, 853, 1013
SsiI CCGC 3 cut(s) 1078, 1208, 1220
SspMI CTAG 4 cut(s) 239, 584, 591, 1283
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 2 cut(s) 259, 343
TaaI ACNGT 3 cut(s) 454, 830, 971
TaiI ACGT 1 cut(s) 1310
TaqI TCGA 2 cut(s) 492, 676
TasI AATT 9 cut(s) 280, 388, 547, 617, 634, 730, 785, 853, 1013
TatI WGTACW 1 cut(s) 1398
TfiI GAWTC 4 cut(s) 796, 983, 1047, 1060
Tru1I TTAA 5 cut(s) 48, 476, 579, 1241, 1274
Tru9I TTAA 5 cut(s) 48, 476, 579, 1241, 1274
TscAI CASTG 5 cut(s) 64, 337, 457, 695, 1123
TseFI GTSAC 1 cut(s) 830
TseI GCWGC 4 cut(s) 438, 486, 1198, 1358
Tsp45I GTSAC 1 cut(s) 830
TspDTI ATGAA 9 cut(s) 126, 174, 316, 662, 932, 957, 1206, 1281, 1281
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 5 cut(s) 64, 337, 457, 695, 1123
VpaK11BI GGWCC 1 cut(s) 1210
XapI RAATTY 3 cut(s) 388, 853, 1013
XceI RCATGY 2 cut(s) 87, 811
XspI CTAG 4 cut(s) 239, 584, 591, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.