RchiOBHm_Chr2g0139531

Folic acid synthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006764
Physical Location & Seq
Forward (+)
57100975 .. 57104639
3665 bp
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UTR
Exon/CDS
Intron
PRQ51007

Sequence Viewer

Length: 453 bp
ATGCATATATTCAAGCAGCTTCTGCCTGACATAAGTCGATTAAATGGTGCCAAAAAGTATTTTCAAGCATCATGTTTTGCCTTTATACATTCAAGCCCAAACTCCCCAGTAGAAGTCCATTCTCAGGATCAGGAAGTAGTGATTGCTTTGGGAAGCAATGTGGGTGACAGACTACATAATTTCAATGAAGCCTTGCAGTTGATGAAAAAATCAGTTAGAGCGGTTACAAAACTTGGGCCTCATGAGCTATTAGGAGCACTGAAGAAAATTGAAAAGGACATGGGTCGTACCCAAGGTATAAGGTATGGTCCCAGGCCTATTGACTTGGATATATTGTTTTACGGGAAGTTAAGAGTCAGATCTGAGATTCTTACAATCCCTCATGAAAGAATTTGGGAAAGACCGTTTGTAATATATAGCCCCATTATTGGATTTGCTGGGATCAGCTATTGA

Protein Analysis

150

Amino Acids

17.04

Weight (kDa)

9.59

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPPK PF01288 72 - 139 1.8e-17 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccBSI CCGCTC 1 cut(s) 221
AciI CCGC 1 cut(s) 221
AclWI GGATC 2 cut(s) 135, 449
AcsI RAATTY 1 cut(s) 390
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 2 cut(s) 124, 428
AgsI TTSAA 5 cut(s) 13, 65, 93, 184, 272
AjnI CCWGG 1 cut(s) 311
AluBI AGCT 3 cut(s) 19, 247, 447
AluI AGCT 3 cut(s) 19, 247, 447
Alw21I GWGCWC 1 cut(s) 259
AlwI GGATC 2 cut(s) 135, 449
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 2 cut(s) 236, 314
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 390
AspS9I GGNCC 2 cut(s) 236, 308
AsuHPI GGTGA 1 cut(s) 176
AvaII GGWCC 1 cut(s) 308
BanI GGYRCC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 313
BglII AGATCT 1 cut(s) 359
BisI GCNGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 18
Bme1390I CCNGG 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 236, 308
BmiI GGNNCC 2 cut(s) 49, 310
BmrFI CCNGG 1 cut(s) 313
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 77
BmuI ACTGGG 1 cut(s) 101
BoxI GACNNNNGTC 2 cut(s) 33, 282
BsaJI CCNNGG 2 cut(s) 292, 311
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 428
Bse1I ACTGG 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 313
BseDI CCNNGG 2 cut(s) 292, 311
BseLI CCNNNNNNNGG 2 cut(s) 124, 428
BseMI GCAATG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 137, 354
BseNI ACTGG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 437
BshFI GGCC 2 cut(s) 238, 316
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 259
BslFI GGGAC 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 124, 428
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 2 cut(s) 238, 316
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 3 cut(s) 127, 359, 441
BspACI CCGC 1 cut(s) 221
BspANI GGCC 2 cut(s) 238, 316
BspCNI CTCAG 2 cut(s) 136, 355
BspHI TCATGA 2 cut(s) 241, 382
BspLI GGNNCC 2 cut(s) 49, 310
BspPI GGATC 2 cut(s) 135, 449
BspT107I GGYRCC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 163
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 292, 311
BssMI GATC 3 cut(s) 127, 359, 441
BssT1I CCWWGG 1 cut(s) 292
Bst2UI CCWGG 1 cut(s) 313
Bst4CI ACNGT 1 cut(s) 405
BstAPI GCANNNNNTGC 1 cut(s) 22
BstDEI CTNAG 2 cut(s) 123, 363
BstKTI GATC 3 cut(s) 130, 362, 444
BstMBI GATC 3 cut(s) 127, 359, 441
BstMWI GCNNNNNNNGC 2 cut(s) 22, 244
BstNI CCWGG 1 cut(s) 313
BstPAI GACNNNNGTC 2 cut(s) 33, 282
BstSCI CCNGG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BsuRI GGCC 2 cut(s) 238, 316
BtsIMutI CAGTG 1 cut(s) 257
CaiI CAGNNNCTG 1 cut(s) 22
CciI TCATGA 2 cut(s) 241, 382
Cfr13I GGNCC 2 cut(s) 236, 308
Csp6I GTAC 1 cut(s) 288
CviAII CATG 4 cut(s) 72, 242, 280, 383
CviJI RGCY 8 cut(s) 19, 96, 191, 238, 247, 316, 420, 447
CviKI_1 RGCY 8 cut(s) 19, 96, 191, 238, 247, 316, 420, 447
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 2 cut(s) 123, 363
DpnI GATC 3 cut(s) 129, 361, 443
DpnII GATC 3 cut(s) 127, 359, 441
Eco130I CCWWGG 1 cut(s) 292
Eco147I AGGCCT 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 311
EcoT14I CCWWGG 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 4 cut(s) 75, 245, 283, 386
FaqI GGGAC 1 cut(s) 294
FatI CATG 4 cut(s) 71, 241, 279, 382
Fnu4HI GCNGC 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 17
GluI GCNGC 1 cut(s) 17
GsaI CCCAGC 1 cut(s) 441
HaeIII GGCC 2 cut(s) 238, 316
Hin1II CATG 4 cut(s) 75, 245, 283, 386
HinfI GANTC 2 cut(s) 354, 367
HphI GGTGA 1 cut(s) 176
Hpy188I TCNGA 2 cut(s) 359, 364
Hpy188III TCNNGA 4 cut(s) 125, 131, 242, 383
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4V TGCA 2 cut(s) 4, 196
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 244
HpyF3I CTNAG 2 cut(s) 123, 363
Hsp92II CATG 4 cut(s) 75, 245, 283, 386
Kzo9I GATC 3 cut(s) 127, 359, 441
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 7 cut(s) 39, 110, 116, 120, 298, 325, 423
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 1 cut(s) 77
MaeIII GTNAC 2 cut(s) 164, 223
MalI GATC 3 cut(s) 129, 361, 443
MbiI CCGCTC 1 cut(s) 221
MboI GATC 3 cut(s) 127, 359, 441
MboII GAAGA 1 cut(s) 274
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 3 cut(s) 178, 267, 390
MlyI GAGTC 1 cut(s) 363
MnlI CCTC 2 cut(s) 249, 390
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 41, 350
MspR9I CCNGG 1 cut(s) 313
MvaI CCWGG 1 cut(s) 313
MwoI GCNNNNNNNGC 2 cut(s) 22, 244
NdeII GATC 3 cut(s) 127, 359, 441
NlaIII CATG 4 cut(s) 75, 245, 283, 386
NlaIV GGNNCC 2 cut(s) 49, 310
NmuCI GTSAC 1 cut(s) 164
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 2 cut(s) 241, 382
PceI AGGCCT 1 cut(s) 316
PfeI GAWTC 1 cut(s) 367
PkrI GCNGC 1 cut(s) 18
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PshAI GACNNNNGTC 2 cut(s) 33, 282
Psp6I CCWGG 1 cut(s) 311
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 1 cut(s) 311
PspN4I GGNNCC 2 cut(s) 49, 310
PspPI GGNCC 2 cut(s) 236, 308
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 1 cut(s) 359
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 41, 350
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 3 cut(s) 127, 359, 441
Sau96I GGNCC 2 cut(s) 236, 308
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 313
SduI GDGCHC 1 cut(s) 259
SetI ASST 5 cut(s) 21, 249, 298, 305, 449
SfaNI GCATC 1 cut(s) 77
SinI GGWCC 1 cut(s) 308
Sse9I AATT 3 cut(s) 178, 267, 390
SseBI AGGCCT 1 cut(s) 316
SsiI CCGC 1 cut(s) 221
StuI AGGCCT 1 cut(s) 316
StyD4I CCNGG 1 cut(s) 311
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 1 cut(s) 405
TaqI TCGA 1 cut(s) 37
TasI AATT 3 cut(s) 178, 267, 390
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 2 cut(s) 41, 350
Tru9I TTAA 2 cut(s) 41, 350
TscAI CASTG 1 cut(s) 264
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 3 cut(s) 201, 218, 399
TspRI CASTG 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 1 cut(s) 390
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.