RchiOBHm_Chr2g0139541

Folic acid synthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006764
Physical Location & Seq
Forward (+)
57104641 .. 57106229
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51008

Sequence Viewer

Length: 972 bp
ATGCATTCTGGTGGGCTTTTTGAGTCTTGGGAGAAGTTGGGCGGTGATTCCCTCATTGGAAAAGAAGGTCTCAAGAGGGTTTTGCCCATTGGAAATGGCTTGTGGGACTGGTCAATGAAAACCTCGGTCATGGGAATCCTTAATCTGACTCCAGATAGTTTTAGTGATGGAGGAAAATTTGAGTCTGTGGAGGCAGCGGTTTCTCAGGTTCGATTGATGATTTCAGAAGGGGCAGATATGATTGATATTGGTGCACAGTCAACGCGGCCAATGGCTTCTAGAATTTCAGTTGAACAAGAATTGGATAGACTAATCCCAGTCCTAGAAGCAGTTGTTGGGATGCCTGAAGTAGAGGGCAAGCTTATATCTGTGGATACATTTTATTCAGAAGTTGCTTCTGAAGCAGTTAGTAAAGGGTCTCATATTGTTAATGATGTATCTGCTGGTCAGTTGGACTCTAACATGCTTAATGTTGTTGCAGGCCTTAGGGTACCTTACATTGCAATGCACATGAGGGGGGATCCTTCTACAATGCAGAACAGTGAAAACCTTCAATATGATGATGTTTGTAAGCAGGTTGCCTCTGAACTATTATTGAGGGTGAGAGATGCAGAAGTATTAGGTATCCCAGCTTGGAGGATAATCATTGACCCTGGAATTGGATTTTCAAAGAATACTGAACAGAGTCTGGATATCTTAATGGGGTTACCAAATATTCAAGAGGAAATTGGGAGGAAAAGTTTGGCATTATCTCATGCACCCATAATGATCGGATCTTCGAGAAAGAAATTCTTAGGTGAAATATGTAATCGCAAGGCTGCGATTGAGAGGGATCCTGCTACTGTTGCTTCTGTCACTGCTGGAGTTTTGGGCGGAGCAAATATTGTAAGAGTGCACAATGTGAGGGATAATCTAGATGCTGTGAAGCTATGTGATGCTATGCTGAGACGGAAGAGATCTTTTCCTCAATAA

Protein Analysis

323

Amino Acids

35.01

Weight (kDa)

5.39

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_bind PF00809 44 - 299 4.3e-82 Pterin binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 490
AccII CGCG 1 cut(s) 265
AciI CCGC 4 cut(s) 42, 197, 265, 873
AclWI GGATC 5 cut(s) 515, 528, 781, 827, 840
AcoI YGGCCR 1 cut(s) 266
AcsI RAATTY 3 cut(s) 176, 282, 788
AcuI CTGAAG 2 cut(s) 366, 420
AdeI CACNNNGTG 1 cut(s) 901
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 1 cut(s) 659
AgsI TTSAA 4 cut(s) 293, 554, 669, 719
AjnI CCWGG 1 cut(s) 652
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 3 cut(s) 361, 632, 928
AluI AGCT 3 cut(s) 361, 632, 928
Alw21I GWGCWC 2 cut(s) 256, 897
Alw26I GTCTC 3 cut(s) 74, 423, 940
Alw44I GTGCAC 2 cut(s) 252, 893
AlwI GGATC 5 cut(s) 515, 528, 781, 827, 840
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 2 cut(s) 266, 481
ApaLI GTGCAC 2 cut(s) 252, 893
ApeKI GCWGC 2 cut(s) 194, 818
ApoI RAATTY 3 cut(s) 176, 282, 788
Asp700I GAANNNNTTC 1 cut(s) 549
Asp718I GGTACC 1 cut(s) 490
AsuHPI GGTGA 3 cut(s) 56, 613, 809
AxyI CCTNAGG 1 cut(s) 485
BaeGI GKGCMC 2 cut(s) 256, 897
BamHI GGATCC 2 cut(s) 520, 832
BanI GGYRCC 1 cut(s) 490
Bbv12I GWGCWC 2 cut(s) 256, 897
BbvI GCAGC 2 cut(s) 206, 805
BccI CCATC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 654
BciVI GTATCC 2 cut(s) 367, 635
BcoDI GTCTC 3 cut(s) 74, 423, 940
BfaI CTAG 3 cut(s) 279, 323, 914
BfuAI ACCTGC 1 cut(s) 565
BfuI GTATCC 2 cut(s) 367, 635
BglII AGATCT 1 cut(s) 956
BisI GCNGC 3 cut(s) 195, 266, 819
BlsI GCNGC 3 cut(s) 196, 267, 820
Bme1390I CCNGG 1 cut(s) 654
BmiI GGNNCC 3 cut(s) 492, 522, 834
BmrFI CCNGG 1 cut(s) 654
BmrI ACTGGG 1 cut(s) 311
BmsI GCATC 4 cut(s) 330, 598, 907, 925
BmuI ACTGGG 1 cut(s) 311
BpmI CTGGAG 2 cut(s) 135, 882
BpuEI CTTGAG 1 cut(s) 56
BsaI GGTCTC 2 cut(s) 74, 423
BsaJI CCNNGG 2 cut(s) 123, 652
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 1 cut(s) 659
Bse1I ACTGG 2 cut(s) 113, 317
Bse21I CCTNAGG 1 cut(s) 485
Bse3DI GCAATG 2 cut(s) 498, 510
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 2 cut(s) 123, 652
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 659
BseMI GCAATG 2 cut(s) 498, 510
BseMII CTCAG 2 cut(s) 218, 935
BseNI ACTGG 2 cut(s) 113, 317
BseSI GKGCMC 2 cut(s) 256, 897
BseXI GCAGC 2 cut(s) 206, 805
BseYI CCCAGC 1 cut(s) 628
Bsh1236I CGCG 1 cut(s) 265
BshFI GGCC 2 cut(s) 268, 483
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 2 cut(s) 256, 897
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 659
BsmAI GTCTC 3 cut(s) 74, 423, 940
BsmBI CGTCTC 1 cut(s) 940
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 2 cut(s) 268, 483
Bso31I GGTCTC 2 cut(s) 74, 423
Bsp1286I GDGCHC 2 cut(s) 256, 897
Bsp143I GATC 5 cut(s) 520, 768, 773, 832, 956
BspACI CCGC 4 cut(s) 42, 197, 265, 873
BspANI GGCC 2 cut(s) 268, 483
BspCNI CTCAG 2 cut(s) 217, 936
BspFNI CGCG 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 492, 522, 834
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 5 cut(s) 515, 528, 781, 827, 840
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 2 cut(s) 74, 423
BsrDI GCAATG 2 cut(s) 498, 510
BsrI ACTGG 2 cut(s) 113, 317
BssECI CCNNGG 2 cut(s) 123, 652
BssMI GATC 5 cut(s) 520, 768, 773, 832, 956
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 3 cut(s) 258, 542, 844
Bst6I CTCTTC 1 cut(s) 947
BstC8I GCNNGC 2 cut(s) 359, 481
BstDEI CTNAG 4 cut(s) 204, 485, 793, 944
BstEII GGTNACC 1 cut(s) 705
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 265
BstKTI GATC 5 cut(s) 523, 771, 776, 835, 959
BstMAI GTCTC 3 cut(s) 74, 423, 940
BstMBI GATC 5 cut(s) 520, 768, 773, 832, 956
BstMWI GCNNNNNNNGC 2 cut(s) 401, 845
BstNI CCWGG 1 cut(s) 654
BstNSI RCATGY 1 cut(s) 466
BstPI GGTNACC 1 cut(s) 705
BstSCI CCNGG 1 cut(s) 652
BstSLI GKGCMC 2 cut(s) 256, 897
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 2 cut(s) 206, 805
BstX2I RGATCY 4 cut(s) 520, 773, 832, 956
BstYI RGATCY 4 cut(s) 520, 773, 832, 956
Bsu36I CCTNAGG 1 cut(s) 485
BsuI GTATCC 2 cut(s) 367, 635
BsuRI GGCC 2 cut(s) 268, 483
BtsCI GGATG 1 cut(s) 345
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 2 cut(s) 547, 855
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 359, 481
CaiI CAGNNNCTG 1 cut(s) 688
Csp6I GTAC 1 cut(s) 491
CviAII CATG 4 cut(s) 130, 463, 511, 755
CviJI RGCY 9 cut(s) 16, 99, 268, 275, 361, 483, 632, 818, 928
CviKI_1 RGCY 9 cut(s) 16, 99, 268, 275, 361, 483, 632, 818, 928
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 4 cut(s) 204, 485, 793, 944
DpnI GATC 5 cut(s) 522, 770, 775, 834, 958
DpnII GATC 5 cut(s) 520, 768, 773, 832, 956
DraIII CACNNNGTG 1 cut(s) 901
EaeI YGGCCR 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 947
EarI CTCTTC 1 cut(s) 947
EciI GGCGGA 1 cut(s) 888
Eco147I AGGCCT 1 cut(s) 483
Eco31I GGTCTC 2 cut(s) 74, 423
Eco32I GATATC 1 cut(s) 694
Eco57I CTGAAG 2 cut(s) 366, 420
Eco81I CCTNAGG 1 cut(s) 485
Eco91I GGTNACC 1 cut(s) 705
EcoO65I GGTNACC 1 cut(s) 705
EcoRII CCWGG 1 cut(s) 652
EcoRV GATATC 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 940
FaeI CATG 4 cut(s) 133, 466, 514, 758
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FaqI GGGAC 1 cut(s) 119
FatI CATG 4 cut(s) 129, 462, 510, 754
Fnu4HI GCNGC 3 cut(s) 195, 266, 819
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 3 cut(s) 195, 266, 819
FspBI CTAG 3 cut(s) 279, 323, 914
GluI GCNGC 3 cut(s) 195, 266, 819
GsaI CCCAGC 1 cut(s) 632
GsuI CTGGAG 2 cut(s) 135, 882
HaeIII GGCC 2 cut(s) 268, 483
Hin1II CATG 4 cut(s) 133, 466, 514, 758
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 7 cut(s) 23, 47, 135, 148, 182, 455, 685
HphI GGTGA 3 cut(s) 56, 613, 809
Hpy166II GTNNAC 3 cut(s) 254, 261, 895
Hpy188I TCNGA 6 cut(s) 147, 226, 388, 400, 586, 773
Hpy188III TCNNGA 7 cut(s) 73, 152, 279, 689, 719, 780, 914
Hpy8I GTNNAC 3 cut(s) 254, 261, 895
HpyAV CCTTC 4 cut(s) 59, 221, 534, 560
HpyCH4III ACNGT 3 cut(s) 258, 542, 844
HpyCH4V TGCA 9 cut(s) 4, 254, 479, 503, 508, 535, 611, 758, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 845
HpyF3I CTNAG 4 cut(s) 204, 485, 793, 944
Hsp92II CATG 4 cut(s) 133, 466, 514, 758
KpnI GGTACC 1 cut(s) 494
Kzo9I GATC 5 cut(s) 520, 768, 773, 832, 956
LmnI GCTCC 1 cut(s) 875
Lsp1109I GCAGC 2 cut(s) 206, 805
LweI GCATC 4 cut(s) 330, 598, 907, 925
MaeI CTAG 3 cut(s) 279, 323, 914
MaeIII GTNAC 2 cut(s) 705, 853
MalI GATC 5 cut(s) 522, 770, 775, 834, 958
MboI GATC 5 cut(s) 520, 768, 773, 832, 956
MboII GAAGA 2 cut(s) 768, 964
MflI RGATCY 4 cut(s) 520, 773, 832, 956
MhlI GDGCHC 2 cut(s) 256, 897
MluCI AATT 6 cut(s) 176, 282, 299, 657, 726, 788
MlyI GAGTC 5 cut(s) 32, 142, 191, 449, 694
MmeI TCCRAC 1 cut(s) 432
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 4 cut(s) 141, 429, 468, 698
MslI CAYNNNNRTG 2 cut(s) 9, 503
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 2 cut(s) 401, 845
NdeII GATC 5 cut(s) 520, 768, 773, 832, 956
NlaIII CATG 4 cut(s) 133, 466, 514, 758
NlaIV GGNNCC 3 cut(s) 492, 522, 834
NmuCI GTSAC 1 cut(s) 853
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 466
PceI AGGCCT 1 cut(s) 483
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 2 cut(s) 47, 135
PkrI GCNGC 3 cut(s) 196, 267, 820
PleI GAGTC 5 cut(s) 31, 142, 190, 449, 693
PpsI GAGTC 5 cut(s) 31, 142, 190, 449, 693
Psp6I CCWGG 1 cut(s) 652
PspEI GGTNACC 1 cut(s) 705
PspFI CCCAGC 1 cut(s) 628
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 3 cut(s) 492, 522, 834
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 4 cut(s) 520, 773, 832, 956
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
RseI CAYNNNNRTG 2 cut(s) 9, 503
SaqAI TTAA 4 cut(s) 141, 429, 468, 698
SatI GCNGC 3 cut(s) 195, 266, 819
Sau3AI GATC 5 cut(s) 520, 768, 773, 832, 956
SchI GAGTC 5 cut(s) 32, 142, 191, 449, 694
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 2 cut(s) 256, 897
SfaNI GCATC 4 cut(s) 330, 598, 907, 925
SmiMI CAYNNNNRTG 2 cut(s) 9, 503
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 6 cut(s) 176, 282, 299, 657, 726, 788
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 4 cut(s) 42, 197, 265, 873
SspI AATATT 2 cut(s) 715, 883
SspMI CTAG 3 cut(s) 279, 323, 914
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 258, 542, 844
TaqI TCGA 2 cut(s) 211, 779
TaqII GACCGA 1 cut(s) 115
TasI AATT 6 cut(s) 176, 282, 299, 657, 726, 788
TauI GCSGC 1 cut(s) 268
TfiI GAWTC 2 cut(s) 47, 135
Tru1I TTAA 4 cut(s) 141, 429, 468, 698
Tru9I TTAA 4 cut(s) 141, 429, 468, 698
TscAI CASTG 2 cut(s) 547, 862
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 2 cut(s) 194, 818
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 1 cut(s) 131
TspGWI ACGGA 1 cut(s) 964
TspRI CASTG 2 cut(s) 547, 862
VneI GTGCAC 2 cut(s) 252, 893
XapI RAATTY 3 cut(s) 176, 282, 788
XbaI TCTAGA 2 cut(s) 278, 913
XceI RCATGY 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 3 cut(s) 279, 323, 914
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.