RchiOBHm_Chr2g0164041

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015337
Physical Location & Seq
Forward (+)
79272945 .. 79274783
1839 bp
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UTR
Exon/CDS
Intron
PRQ53217

Sequence Viewer

Length: 480 bp
ATGGGCAATACCCTTAGAGGCGGGGGTACCGAGGAGAGGACTAGTGATATTAGTATCATAGCAGATGACTACTACGAGGCCAATATCAAAGGCAATGAATCAACTCAATCAAGCATAGCTGAGAGTGGGTTCTACAGAGCTATATGCGAAATCGTCGAAAAAATAAACCATCAACATAATAACACACAAATTAACGTGCCAACGGAAAAGAAACTCAAGGAAGCATTTAAGGCTAACCACAGAGAAGGGACAGGAAAATTGACAAAGGAAGAGTTCGGAAGATGTGTGCAACAAGTGATAAGGGAAGCAGGGATCAGTGGTTATGGAGCCAAGGACTCACTTATTTACATCTTCGGGGTTCCCATAACTGCACTCTTAATCAAGCAATCAGTAATGCCTCGTGCAGTTTCGAATGACTACTTCATTCCAGGAGTCACTTCCGCAACTGCCTTGGTGCTCGCCAAACTTAACAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.42

Weight (kDa)

7.75

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 21 - 159 9.9e-52 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41200
fragaria_vesca FvH4_6g45840 FvH4_6g45840
malus_domestica MD14G1087900.v1.1
prunus_persica Prupe.7G038600_v2.0.a1 Prupe.7G038600_v2.0.a1
pyrus_communis pycom14g07800
rosa_chinensis RchiOBHm_Chr2g0164041
rosa_laevigata RLG00000021434
rosa_multiflora Rmu_ssc0000432.1_g000009
rosa_roxburghii Rroxscaffold_2G00086810
rosa_samantha Rh2AG574500 Rh2BG585100 Rh2CG556000 Rh2DG594700
rosa_wichuraiana Rw2G047520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 26
AciI CCGC 2 cut(s) 21, 441
AclWI GGATC 1 cut(s) 320
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 2 cut(s) 119, 140
AluI AGCT 2 cut(s) 119, 140
Alw21I GWGCWC 1 cut(s) 459
AlwI GGATC 1 cut(s) 320
AoxI GGCC 1 cut(s) 78
Asp718I GGTACC 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 410
BanI GGYRCC 1 cut(s) 26
BauI CACGAG 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 459
BccI CCATC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 429
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 429
BmiI GGNNCC 3 cut(s) 28, 328, 360
BmrFI CCNGG 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 3 cut(s) 30, 330, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 30, 330, 450
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 47
BsgI GTGCAG 2 cut(s) 354, 423
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 459
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 1 cut(s) 312
BspACI CCGC 2 cut(s) 21, 441
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 3 cut(s) 28, 328, 360
BspPI GGATC 1 cut(s) 320
BspT104I TTCGAA 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 30, 330, 450
BssMI GATC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 2 cut(s) 330, 450
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 429
Bst6I CTCTTC 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 14, 120
BstKTI GATC 1 cut(s) 315
BstMBI GATC 1 cut(s) 312
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 133
BsuRI GGCC 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 459
Csp6I GTAC 1 cut(s) 27
CviJI RGCY 5 cut(s) 80, 119, 140, 233, 329
CviKI_1 RGCY 5 cut(s) 80, 119, 140, 233, 329
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 2 cut(s) 14, 120
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 2 cut(s) 330, 450
EcoRII CCWGG 1 cut(s) 427
EcoT14I CCWWGG 2 cut(s) 330, 450
ErhI CCWWGG 2 cut(s) 330, 450
FaiI YATR 7 cut(s) 59, 116, 143, 145, 177, 324, 365
FaqI GGGAC 1 cut(s) 262
FauI CCCGC 1 cut(s) 14
FspBI CTAG 1 cut(s) 42
HaeIII GGCC 1 cut(s) 80
HinfI GANTC 3 cut(s) 98, 335, 432
Hpy188I TCNGA 1 cut(s) 278
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 3 cut(s) 289, 371, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 2 cut(s) 14, 120
HpySE526I ACGT 1 cut(s) 195
KpnI GGTACC 1 cut(s) 30
Kzo9I GATC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 4 cut(s) 237, 294, 414, 441
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 433
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 3 cut(s) 281, 291, 343
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 2 cut(s) 189, 257
MlyI GAGTC 2 cut(s) 329, 441
MnlI CCTC 5 cut(s) 11, 25, 30, 70, 408
MseI TTAA 5 cut(s) 192, 228, 377, 468, 478
MspR9I CCNGG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 1 cut(s) 230
NdeII GATC 1 cut(s) 312
NlaIV GGNNCC 3 cut(s) 28, 328, 360
NmuCI GTSAC 1 cut(s) 433
NspV TTCGAA 1 cut(s) 410
PfeI GAWTC 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 427
PleI GAGTC 2 cut(s) 329, 440
PpsI GAGTC 2 cut(s) 329, 440
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 3 cut(s) 28, 328, 360
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SaqAI TTAA 5 cut(s) 192, 228, 377, 468, 478
Sau3AI GATC 1 cut(s) 312
SchI GAGTC 2 cut(s) 329, 441
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 1 cut(s) 459
SetI ASST 3 cut(s) 121, 142, 198
SfcI CTRYAG 1 cut(s) 133
SfuI TTCGAA 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 2 cut(s) 189, 257
SsiI CCGC 2 cut(s) 21, 441
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 2 cut(s) 330, 450
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 156, 410
TasI AATT 2 cut(s) 189, 257
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 5 cut(s) 192, 228, 377, 468, 478
Tru9I TTAA 5 cut(s) 192, 228, 377, 468, 478
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 1 cut(s) 433
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 2 cut(s) 111, 412
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 1 cut(s) 322
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.