RLG00000021434

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015337
Physical Location & Seq
Forward (+)
75401869 .. 75403321
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021434

Sequence Viewer

Length: 477 bp
ATGGGCAATATCCTTAGGGGCGGGGGTACCGAGGAGAAGGCTAGTGATATTAGTAGCATAGCATACGAGTACTACGAGGCCAATATCAAAGGCAATGAATCAAGCATAGCTGAGACTGAGAGTGGGTTCTACAGAGCTATATGCGAAATCGTCGAAAAAATAAACAATCAATATAGTAACACGCAAATTAACGTGCCAACGGAGAAGAAACTCAAGGAAGCATTCAAGGCTAACCACAGAGAAGGGACAGGAAAATTGACAAAGGAAGAGTTCGGAAAATGTGTGCAACAAGTGATAAGGGAAGCAGGGATCAGTGGTTATGGAGCCAAGGACTCACTTATTTACATCTTCGGGGTTCCCATAACTGCACTCTTAATCAAGCAATCAGTAATGCCTCGTGCAGTTTCGAATGACTACTTCATTCCAGGAGTCACTTCCGCAACTGCCTTGGTGCTTGCCAAACTTAACAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.28

Weight (kDa)

7.69

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 23 - 158 1.5e-51 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015337)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41200
fragaria_vesca FvH4_6g45840 FvH4_6g45840
malus_domestica MD14G1087900.v1.1
prunus_persica Prupe.7G038600_v2.0.a1 Prupe.7G038600_v2.0.a1
pyrus_communis pycom14g07800
rosa_chinensis RchiOBHm_Chr2g0164041
rosa_laevigata RLG00000021434
rosa_multiflora Rmu_ssc0000432.1_g000009
rosa_roxburghii Rroxscaffold_2G00086810
rosa_samantha Rh2AG574500 Rh2BG585100 Rh2CG556000 Rh2DG594700
rosa_wichuraiana Rw2G047520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 26
AciI CCGC 2 cut(s) 21, 438
AclWI GGATC 1 cut(s) 317
AfaI GTAC 2 cut(s) 28, 71
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 2 cut(s) 110, 137
AluI AGCT 2 cut(s) 110, 137
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 1 cut(s) 317
AoxI GGCC 1 cut(s) 78
Asp718I GGTACC 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 407
AxyI CCTNAGG 1 cut(s) 14
BanI GGYRCC 1 cut(s) 26
BauI CACGAG 1 cut(s) 396
BciT130I CCWGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 130
BmcAI AGTACT 1 cut(s) 71
Bme1390I CCNGG 1 cut(s) 426
BmiI GGNNCC 3 cut(s) 28, 325, 357
BmrFI CCNGG 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 197
BsaJI CCNNGG 3 cut(s) 30, 327, 447
Bse21I CCTNAGG 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 30, 327, 447
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 2 cut(s) 102, 108
BseRI GAGGAG 1 cut(s) 47
BsgI GTGCAG 2 cut(s) 351, 420
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 407
Bsp143I GATC 1 cut(s) 309
BspACI CCGC 2 cut(s) 21, 438
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 103, 109
BspLI GGNNCC 3 cut(s) 28, 325, 357
BspPI GGATC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 30, 327, 447
BssMI GATC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 2 cut(s) 327, 447
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 426
Bst6I CTCTTC 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 3 cut(s) 14, 111, 117
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 309
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 1 cut(s) 130
Bsu36I CCTNAGG 1 cut(s) 14
BsuRI GGCC 1 cut(s) 80
BtsIMutI CAGTG 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 456
Csp6I GTAC 2 cut(s) 27, 70
CviJI RGCY 6 cut(s) 41, 80, 110, 137, 230, 326
CviKI_1 RGCY 6 cut(s) 41, 80, 110, 137, 230, 326
CviQI GTAC 2 cut(s) 27, 70
DdeI CTNAG 3 cut(s) 14, 111, 117
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 327, 447
Eco81I CCTNAGG 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 327, 447
ErhI CCWWGG 2 cut(s) 327, 447
FaiI YATR 8 cut(s) 59, 64, 107, 140, 142, 174, 321, 362
FaqI GGGAC 1 cut(s) 259
FauI CCCGC 1 cut(s) 14
FspBI CTAG 1 cut(s) 42
HaeIII GGCC 1 cut(s) 80
HinfI GANTC 3 cut(s) 98, 332, 429
Hpy188I TCNGA 1 cut(s) 275
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 31, 236
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 286, 368, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 3 cut(s) 14, 111, 117
HpySE526I ACGT 1 cut(s) 192
KpnI GGTACC 1 cut(s) 30
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 4 cut(s) 234, 291, 411, 438
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 176, 430
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 3 cut(s) 217, 278, 340
MluCI AATT 2 cut(s) 186, 254
MlyI GAGTC 2 cut(s) 326, 438
MnlI CCTC 3 cut(s) 25, 70, 405
MseI TTAA 4 cut(s) 189, 374, 465, 475
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeII GATC 1 cut(s) 309
NlaIV GGNNCC 3 cut(s) 28, 325, 357
NmuCI GTSAC 1 cut(s) 430
NspV TTCGAA 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 424
PleI GAGTC 2 cut(s) 326, 437
PpsI GAGTC 2 cut(s) 326, 437
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 3 cut(s) 28, 325, 357
RsaI GTAC 2 cut(s) 28, 71
RsaNI GTAC 2 cut(s) 27, 70
SaqAI TTAA 4 cut(s) 189, 374, 465, 475
Sau3AI GATC 1 cut(s) 309
ScaI AGTACT 1 cut(s) 71
SchI GAGTC 2 cut(s) 326, 438
ScrFI CCNGG 1 cut(s) 426
SetI ASST 3 cut(s) 112, 139, 195
SfcI CTRYAG 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 2 cut(s) 186, 254
SsiI CCGC 2 cut(s) 21, 438
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 424
StyI CCWWGG 2 cut(s) 327, 447
TaiI ACGT 1 cut(s) 195
TaqI TCGA 2 cut(s) 153, 407
TasI AATT 2 cut(s) 186, 254
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 4 cut(s) 189, 374, 465, 475
Tru9I TTAA 4 cut(s) 189, 374, 465, 475
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 430
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 111, 409
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 1 cut(s) 319
XspI CTAG 1 cut(s) 42
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.