Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0016077

Physical Location & Seq

Genomic Coordinates Reverse (-)

388418 .. 390074

Gene Structure 1657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41533

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGAACCAGCTCCATTACTGGAAACACCAACTTTAGACCCTTATGATGATGAAGAAGACGAAGACAAGTATCTGATACCAAAGCCATCAGAGTGGTTCCCAAAGCTAGTGTCTTTACAAGCTGATTTGATTTACAACTCCATCCTGGTTTTATGTCACCCCATACTCTCCATCTTCTCTTTCGCCTACGAGTCTTACCACCAAGCCGAGCAGGCCAAGGAGACGGTCGAGTCCGCAGTCCAAAAAGCCCCCTCCACCATCATACACGGCGCAGTCGTCCTCATCAAGAAGCTCGGCTTTGGAGTTCTCGGGGCAGCCTATGTCTGCATGGTGTTGTTTATGCTTTTGATTCTGGCCACAATTGTGGGAGTTGCTTTGGTGCGGCTCTGGCTGGAAGAGCCTGTGGTTGTTAGGGATAGGTTGCACTTTGATTACACCCTAGCTCATCCCAAGGCGGTGTTTGTGTTCGGAGGATCAGGGTTAGCTGCTGGGAGTGGTGCTGACTACTTCAAGATTATTATTAGCAGCAACAGTAGGCGTATGGCTATGGGGGTTCCTGTTGGACACACTTTTACTGTGTCTCTGGAGCTTTTGATGCCGGAGTCTGATTTTAATAGGCACATTGGGGTGTTTCAGATGACCGCAGAAGCATTAGCAGCTAATGGTGATGTGATTGCCAAGTCAAGCCAGCCATGTATGCTACGATTTAGAAGCCTCCCAATTCGACTCACAAGAACATTTCTTATGGGTGTACCTCTGATACTGGGCATTTCTGGAGAAACCCAGAAGATAGCTGGACAAATACTGAGGCACAAGGAAGGGACTTATCCAAGAACCCGAGCAATAAGAATAACATTAAGTCCAAGAGCTGGTACTTCACATCTTCCACAGTTGTATGAGGCTGAAATTGTTTTGAATTCACAGCTGCCTTGGACAAAGCAATTGGTGCGCAGTTGGAAATGGACGCTCTATGTCTGGGCCTCTTTGTATTGTTACACTATATTCGTAATAACTTATGTAACGTGTTGTAGACCACTCTTGTCACCAGTGACAATGATCAAAACTGCGGCGAATTATGTTGCTAATCGTGTTAATCATGACCGATCAACTACTGATCATGTTCAAAGAGATAGAGGGGCGAGTACTGAAGTAAGACGGCGAAGACCCCTTCGAGTGCCTAAAGATGCCACAACGACACTTAAAGATGATTCTGATCAGAGTGAGGATATTTCAGAGTTGTTGAGAAAGTGGCAGCGGAGCAGAAGCAAAAGAAAGGCAATCTATTTGCAAACGGATTTACCAAGTGAGACTGTTGGCACCTCATCTGCACCATCAACGATTAGTATGAGTACTCGAGAAGATGCAAGTACTGTAACTGCGGAAGAAGATGTTGGGGACTCGGAATCAGTGTGTACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006996 GO:0007275 GO:0008150 GO:0009791 GO:0009846 GO:0009856 GO:0009987 GO:0010154 GO:0010162 GO:0010344 GO:0010431 GO:0010876 GO:0012505 GO:0016043 GO:0019915 GO:0021700 GO:0022414 GO:0022607 GO:0022611 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0034389 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0051179 GO:0051235 GO:0051704 GO:0061458 GO:0065007 GO:0065008 GO:0070925 GO:0071695 GO:0071840 GO:0080154 GO:0080155 GO:0140042 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Seipin

Protein Analysis

472

Amino Acids

52.67

Weight (kDa)

7.64

Isoelectric Point (pI)

51.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Seipin	PF06775	113 - 330	2.5e-46	Putative adipose-regulatory protein (Seipin)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016077)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16460
fragaria_vesca	FvH4_6g00490
malus_domestica	MD04G1244900.v1.1
prunus_persica	Prupe.6G361500_v2.0.a1
pyrus_communis	pycom04g21570
rosa_chinensis	RchiOBHm_Chr3g0447871
rosa_laevigata	RLG00000025965
rosa_multiflora	Rmu_sc0020869.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426070
rosa_rugosa	Rorug02G0606700
rosa_samantha	Rh3AG005800 Rh3BG005600 Rh3CG005700 Rh3DG006000
rosa_wichuraiana	Rw3G000450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	229
Acc16I	TGCGCA	1 cut(s)	950
AccB1I	GGYRCC	1 cut(s)	1316
AccB7I	CCANNNNNTGG	1 cut(s)	869
AccI	GTMKAC	1 cut(s)	1030
AciI	CCGC	7 cut(s)	234, 382, 455, 642, 1067, 1255, 1379
AclWI	GGATC	1 cut(s)	481
AcoI	YGGCCR	1 cut(s)	354
AcsI	RAATTY	1 cut(s)	916
AcuI	CTGAAG	1 cut(s)	1167
AfaI	GTAC	6 cut(s)	753, 874, 1144, 1351, 1369, 1414
AfiI	CCNNNNNNNGG	1 cut(s)	869
AflIII	ACRYGT	1 cut(s)	1022
AgsI	TTSAA	3 cut(s)	511, 916, 1124
AjnI	CCWGG	1 cut(s)	144
AjuI	GAANNNNNNNTTGG	2 cut(s)	1374, 1406
AleI	CACNNNNGTG	1 cut(s)	362
Alw26I	GTCTC	3 cut(s)	215, 585, 1301
AlwI	GGATC	1 cut(s)	481
Ama87I	CYCGRG	3 cut(s)	308, 837, 1353
AoxI	GGCC	3 cut(s)	213, 354, 978
ApeKI	GCWGC	6 cut(s)	314, 485, 525, 656, 925, 1252
ApoI	RAATTY	1 cut(s)	916
ArsI	GACNNNNNNTTYG	2 cut(s)	95, 127
AspLEI	GCGC	2 cut(s)	272, 951
AspS9I	GGNCC	1 cut(s)	978
AsuHPI	GGTGA	3 cut(s)	149, 677, 1035
AvaI	CYCGRG	3 cut(s)	308, 837, 1353
BalI	TGGCCA	1 cut(s)	356
BanI	GGYRCC	1 cut(s)	1316
BbsI	GAAGAC	3 cut(s)	63, 69, 1168
BbvI	GCAGC	6 cut(s)	326, 472, 537, 668, 912, 1264
BccI	CCATC	5 cut(s)	94, 149, 179, 266, 1339
BceAI	ACGGC	2 cut(s)	283, 1172
BciT130I	CCWGG	1 cut(s)	146
BclI	TGATCA	3 cut(s)	1056, 1114, 1213
BcoDI	GTCTC	3 cut(s)	215, 585, 1301
BfaI	CTAG	2 cut(s)	107, 440
BglI	GCCNNNNNGGC	1 cut(s)	212
BisI	GCNGC	8 cut(s)	315, 383, 486, 526, 657, 926, 1068, 1253
BlsI	GCNGC	8 cut(s)	316, 384, 487, 527, 658, 927, 1069, 1254
BmcAI	AGTACT	3 cut(s)	1144, 1351, 1369
Bme1390I	CCNGG	1 cut(s)	146
BmeT110I	CYCGRG	3 cut(s)	308, 837, 1353
BmgT120I	GGNCC	1 cut(s)	978
BmiI	GGNNCC	4 cut(s)	6, 98, 555, 1318
BmrFI	CCNGG	1 cut(s)	146
BmrI	ACTGGG	1 cut(s)	773
BmsI	GCATC	3 cut(s)	585, 1174, 1351
BmuI	ACTGGG	1 cut(s)	773
BpiI	GAAGAC	3 cut(s)	63, 69, 1168
BpmI	CTGGAG	2 cut(s)	605, 795
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaJI	CCNNGG	3 cut(s)	216, 450, 929
Bsc4I	CCNNNNNNNGG	1 cut(s)	869
Bse1I	ACTGG	3 cut(s)	24, 768, 1046
Bse8I	GATNNNNATC	1 cut(s)	1212
BseBI	CCWGG	1 cut(s)	146
BseDI	CCNNGG	3 cut(s)	216, 450, 929
BseGI	GGATG	2 cut(s)	141, 445
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	1 cut(s)	869
BseMII	CTCAG	1 cut(s)	797
BseNI	ACTGG	3 cut(s)	24, 768, 1046
BseXI	GCAGC	6 cut(s)	326, 472, 537, 668, 912, 1264
BseYI	CCCAGC	1 cut(s)	488
BsgI	GTGCAG	1 cut(s)	1311
Bsh1285I	CGRYCG	1 cut(s)	228
BshFI	GGCC	3 cut(s)	215, 356, 980
BshNI	GGYRCC	1 cut(s)	1316
BsiEI	CGRYCG	1 cut(s)	228
BsiHKCI	CYCGRG	3 cut(s)	308, 837, 1353
BsiSI	CCGG	1 cut(s)	599
BslFI	GGGAC	2 cut(s)	835, 1409
BslI	CCNNNNNNNGG	1 cut(s)	869
BsmAI	GTCTC	3 cut(s)	215, 585, 1301
BsmBI	CGTCTC	1 cut(s)	215
BsmFI	GGGAC	2 cut(s)	835, 1409
BsnI	GGCC	3 cut(s)	215, 356, 980
BsoBI	CYCGRG	3 cut(s)	308, 837, 1353
Bsp143I	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
BspACI	CCGC	7 cut(s)	234, 382, 455, 642, 1067, 1255, 1379
BspANI	GGCC	3 cut(s)	215, 356, 980
BspCNI	CTCAG	1 cut(s)	798
BspHI	TCATGA	1 cut(s)	1096
BspLI	GGNNCC	4 cut(s)	6, 98, 555, 1318
BspPI	GGATC	1 cut(s)	481
BspQI	GCTCTTC	1 cut(s)	390
BspT107I	GGYRCC	1 cut(s)	1316
BsrI	ACTGG	3 cut(s)	24, 768, 1046
BssECI	CCNNGG	3 cut(s)	216, 450, 929
BssMI	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
BssT1I	CCWWGG	3 cut(s)	216, 450, 929
Bst2UI	CCWGG	1 cut(s)	146
Bst4CI	ACNGT	6 cut(s)	226, 533, 577, 891, 1312, 1372
Bst6I	CTCTTC	1 cut(s)	390
BstAPI	GCANNNNNTGC	1 cut(s)	946
BstC8I	GCNNGC	2 cut(s)	213, 689
BstDEI	CTNAG	2 cut(s)	806, 1416
BstF5I	GGATG	2 cut(s)	141, 445
BstHHI	GCGC	2 cut(s)	272, 951
BstKTI	GATC	5 cut(s)	476, 1059, 1106, 1117, 1216
BstMAI	GTCTC	3 cut(s)	215, 585, 1301
BstMBI	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
BstMCI	CGRYCG	1 cut(s)	228
BstMWI	GCNNNNNNNGC	7 cut(s)	212, 388, 397, 595, 656, 697, 946
BstNI	CCWGG	1 cut(s)	146
BstSCI	CCNGG	1 cut(s)	144
BstV1I	GCAGC	6 cut(s)	326, 472, 537, 668, 912, 1264
BstV2I	GAAGAC	3 cut(s)	63, 69, 1168
BstXI	CCANNNNNNTGG	2 cut(s)	93, 364
BsuRI	GGCC	3 cut(s)	215, 356, 980
BtsCI	GGATG	2 cut(s)	141, 445
BtsIMutI	CAGTG	2 cut(s)	1053, 1413
Cac8I	GCNNGC	2 cut(s)	213, 689
CciI	TCATGA	1 cut(s)	1096
CfoI	GCGC	2 cut(s)	272, 951
Cfr13I	GGNCC	1 cut(s)	978
CseI	GACGC	1 cut(s)	973
Csp6I	GTAC	6 cut(s)	752, 873, 1143, 1350, 1368, 1413
CviAII	CATG	4 cut(s)	328, 693, 1097, 1118
CviQI	GTAC	6 cut(s)	752, 873, 1143, 1350, 1368, 1413
DdeI	CTNAG	2 cut(s)	806, 1416
DpnI	GATC	5 cut(s)	475, 1058, 1105, 1116, 1215
DpnII	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
DrdI	GACNNNNNNGTC	1 cut(s)	229
DseDI	GACNNNNNNGTC	1 cut(s)	229
EaeI	YGGCCR	1 cut(s)	354
Eam1104I	CTCTTC	1 cut(s)	390
EarI	CTCTTC	1 cut(s)	390
Eco130I	CCWWGG	3 cut(s)	216, 450, 929
Eco57I	CTGAAG	1 cut(s)	1167
Eco88I	CYCGRG	3 cut(s)	308, 837, 1353
EcoRI	GAATTC	1 cut(s)	916
EcoRII	CCWGG	1 cut(s)	144
EcoT14I	CCWWGG	3 cut(s)	216, 450, 929
ErhI	CCWWGG	3 cut(s)	216, 450, 929
Esp3I	CGTCTC	1 cut(s)	215
FaeI	CATG	4 cut(s)	331, 696, 1100, 1121
FalI	AAGNNNNNCTT	2 cut(s)	281, 313
FaqI	GGGAC	2 cut(s)	835, 1409
FatI	CATG	4 cut(s)	327, 692, 1096, 1117
FbaI	TGATCA	3 cut(s)	1056, 1114, 1213
FblI	GTMKAC	1 cut(s)	1030
Fnu4HI	GCNGC	8 cut(s)	315, 383, 486, 526, 657, 926, 1068, 1253
FokI	GGATG	2 cut(s)	128, 432
Fsp4HI	GCNGC	8 cut(s)	315, 383, 486, 526, 657, 926, 1068, 1253
FspBI	CTAG	2 cut(s)	107, 440
FspI	TGCGCA	1 cut(s)	950
GlaI	GCGC	2 cut(s)	271, 950
GluI	GCNGC	8 cut(s)	315, 383, 486, 526, 657, 926, 1068, 1253
GsaI	CCCAGC	1 cut(s)	492
GsuI	CTGGAG	2 cut(s)	605, 795
HaeIII	GGCC	3 cut(s)	215, 356, 980
HapII	CCGG	1 cut(s)	599
HgaI	GACGC	1 cut(s)	973
HhaI	GCGC	2 cut(s)	272, 951
Hin1II	CATG	4 cut(s)	331, 696, 1100, 1121
Hin6I	GCGC	2 cut(s)	270, 949
HinP1I	GCGC	2 cut(s)	270, 949
HinfI	GANTC	8 cut(s)	191, 230, 349, 602, 726, 1208, 1397, 1403
HpaII	CCGG	1 cut(s)	599
HphI	GGTGA	3 cut(s)	149, 677, 1035
Hpy166II	GTNNAC	3 cut(s)	752, 1031, 1413
Hpy188III	TCNNGA	6 cut(s)	286, 511, 584, 774, 1097, 1355
Hpy8I	GTNNAC	3 cut(s)	752, 1031, 1413
HpyAV	CCTTC	2 cut(s)	812, 1178
HpyCH4III	ACNGT	6 cut(s)	226, 533, 577, 891, 1312, 1372
HpyCH4IV	ACGT	1 cut(s)	1022
HpyCH4V	TGCA	5 cut(s)	327, 424, 1288, 1328, 1364
HpyF10VI	GCNNNNNNNGC	7 cut(s)	212, 388, 397, 595, 656, 697, 946
HpyF3I	CTNAG	2 cut(s)	806, 1416
HpySE526I	ACGT	1 cut(s)	1022
Hsp92II	CATG	4 cut(s)	331, 696, 1100, 1121
HspAI	GCGC	2 cut(s)	270, 949
Ksp22I	TGATCA	3 cut(s)	1056, 1114, 1213
Kzo9I	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
LguI	GCTCTTC	1 cut(s)	390
LmnI	GCTCC	3 cut(s)	16, 586, 1257
Lsp1109I	GCAGC	6 cut(s)	326, 472, 537, 668, 912, 1264
LweI	GCATC	3 cut(s)	585, 1174, 1351
MaeI	CTAG	2 cut(s)	107, 440
MaeII	ACGT	1 cut(s)	1022
MaeIII	GTNAC	6 cut(s)	155, 992, 1018, 1041, 1048, 1372
MalI	GATC	5 cut(s)	475, 1058, 1105, 1116, 1215
MboI	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
MfeI	CAATTG	2 cut(s)	360, 941
MlsI	TGGCCA	1 cut(s)	356
MluCI	AATT	6 cut(s)	360, 720, 906, 916, 941, 1072
MluNI	TGGCCA	1 cut(s)	356
MlyI	GAGTC	5 cut(s)	200, 239, 611, 720, 1391
MmeI	TCCRAC	2 cut(s)	541, 935
Mox20I	TGGCCA	1 cut(s)	356
MscI	TGGCCA	1 cut(s)	356
MseI	TTAA	4 cut(s)	612, 857, 1092, 1200
MslI	CAYNNNNRTG	3 cut(s)	91, 362, 626
Msp20I	TGGCCA	1 cut(s)	356
MspA1I	CMGCKG	2 cut(s)	925, 1255
MspI	CCGG	1 cut(s)	599
MspR9I	CCNGG	1 cut(s)	146
MunI	CAATTG	2 cut(s)	360, 941
MvaI	CCWGG	1 cut(s)	146
MwoI	GCNNNNNNNGC	7 cut(s)	212, 388, 397, 595, 656, 697, 946
NdeII	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
NlaIII	CATG	4 cut(s)	331, 696, 1100, 1121
NlaIV	GGNNCC	4 cut(s)	6, 98, 555, 1318
NmeAIII	GCCGAG	2 cut(s)	232, 273
NmuCI	GTSAC	3 cut(s)	155, 1041, 1048
NsbI	TGCGCA	1 cut(s)	950
OliI	CACNNNNGTG	1 cut(s)	362
PaeR7I	CTCGAG	1 cut(s)	1353
PagI	TCATGA	1 cut(s)	1096
PciSI	GCTCTTC	1 cut(s)	390
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PfeI	GAWTC	3 cut(s)	349, 1208, 1403
PflMI	CCANNNNNTGG	1 cut(s)	869
PkrI	GCNGC	8 cut(s)	316, 384, 487, 527, 658, 927, 1069, 1254
PleI	GAGTC	5 cut(s)	199, 238, 610, 720, 1391
PpsI	GAGTC	5 cut(s)	199, 238, 610, 720, 1391
Psp6I	CCWGG	1 cut(s)	144
PspFI	CCCAGC	1 cut(s)	488
PspGI	CCWGG	1 cut(s)	144
PspN4I	GGNNCC	4 cut(s)	6, 98, 555, 1318
PspPI	GGNCC	1 cut(s)	978
PvuII	CAGCTG	1 cut(s)	925
RsaI	GTAC	6 cut(s)	753, 874, 1144, 1351, 1369, 1414
RsaNI	GTAC	6 cut(s)	752, 873, 1143, 1350, 1368, 1413
RseI	CAYNNNNRTG	3 cut(s)	91, 362, 626
SapI	GCTCTTC	1 cut(s)	390
SaqAI	TTAA	4 cut(s)	612, 857, 1092, 1200
SatI	GCNGC	8 cut(s)	315, 383, 486, 526, 657, 926, 1068, 1253
Sau3AI	GATC	5 cut(s)	473, 1056, 1103, 1114, 1213
Sau96I	GGNCC	1 cut(s)	978
ScaI	AGTACT	3 cut(s)	1144, 1351, 1369
SchI	GAGTC	5 cut(s)	200, 239, 611, 720, 1391
ScrFI	CCNGG	1 cut(s)	146
SfaNI	GCATC	3 cut(s)	585, 1174, 1351
Sfr274I	CTCGAG	1 cut(s)	1353
SlaI	CTCGAG	1 cut(s)	1353
SmiMI	CAYNNNNRTG	3 cut(s)	91, 362, 626
SmlI	CTYRAG	1 cut(s)	1353
SmoI	CTYRAG	1 cut(s)	1353
Sse9I	AATT	6 cut(s)	360, 720, 906, 916, 941, 1072
SsiI	CCGC	7 cut(s)	234, 382, 455, 642, 1067, 1255, 1379
SspMI	CTAG	2 cut(s)	107, 440
StyD4I	CCNGG	1 cut(s)	144
StyI	CCWWGG	3 cut(s)	216, 450, 929
TaaI	ACNGT	6 cut(s)	226, 533, 577, 891, 1312, 1372
TaiI	ACGT	1 cut(s)	1025
TaqI	TCGA	4 cut(s)	228, 724, 1171, 1354
TaqII	GACCGA	1 cut(s)	1116
TasI	AATT	6 cut(s)	360, 720, 906, 916, 941, 1072
TatI	WGTACW	4 cut(s)	1142, 1349, 1367, 1412
TauI	GCSGC	2 cut(s)	385, 1070
TfiI	GAWTC	3 cut(s)	349, 1208, 1403
Tru1I	TTAA	4 cut(s)	612, 857, 1092, 1200
Tru9I	TTAA	4 cut(s)	612, 857, 1092, 1200
TscAI	CASTG	2 cut(s)	1053, 1413
TseFI	GTSAC	3 cut(s)	155, 1041, 1048
TseI	GCWGC	6 cut(s)	314, 485, 525, 656, 925, 1252
Tsp45I	GTSAC	3 cut(s)	155, 1041, 1048
TspDTI	ATGAA	1 cut(s)	66
TspGWI	ACGGA	1 cut(s)	1307
TspRI	CASTG	2 cut(s)	1053, 1413
Van91I	CCANNNNNTGG	1 cut(s)	869
XapI	RAATTY	1 cut(s)	916
XcmI	CCANNNNNNNNNTGG	1 cut(s)	791
XhoI	CTCGAG	1 cut(s)	1353
XmiI	GTMKAC	1 cut(s)	1030
XspI	CTAG	2 cut(s)	107, 440
ZrmI	AGTACT	3 cut(s)	1144, 1351, 1369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.