RchiOBHm_Chr3g0457721
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011642
Physical Location & Seq
Reverse (-)
6726357 .. 6729663
3307 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42447

Sequence Viewer

Length: 546 bp
ATGGGGTTGTCTTTCACCAAGTTGTTCAGTCGGCTTTTTGCCAAGAAGGAAATGAGAATCCTTATGGTCGGTCTCGATGCTGCGGGTAAAACTACCATTTTGTACAAGCTCAAGCTCGGAGAGATTGTGACCACCATTCCTACAATCGGATTCAATGTTGAAACCGTGGAGTACAAGAACATTAGCTTTACCGTGTGGGATGTCGGGGGTCAGGACAAGATTCGACCTTTATGGAGACACTACTTCCAAAATACACAAGGGCTCATTTTTGTGGTTGATAGCAATGATCGTGACCGTGTCGTTGAGGCTAGGGATGAGCTGCACCGCATGTTGAATGAGGACGAGTTGAGGGATGCTGTGCTGCTTGTATTTGCCAACAAGCAGGATCTTCCAAATGCTATGAATGCTGCAGAGATCACTGACAAACTTGGTCTCCACTCTCTTCGTCAACGCCACTGGTACATCCAGAGTACATGTGCCACTTCTGGTGAAGGGCTCTATGAGGGTCTTGACTGGCTCTCAAACAATATCGCAAACAAGGCTTAA

Protein Analysis

181

Amino Acids

20.68

Weight (kDa)

6.43

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 5 - 177 1.2e-81 ADP-ribosylation factor family
SRPRB PF09439 16 - 143 4.8e-13 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 8.3e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 173 5e-12 Ras family
Gtr1_RagA PF04670 19 - 141 1.6e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 45 - 111 7.9e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 83, 325
AclWI GGATC 1 cut(s) 393
AfaI GTAC 4 cut(s) 104, 173, 461, 472
AfiI CCNNNNNNNGG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 3 cut(s) 154, 161, 334
AluBI AGCT 4 cut(s) 109, 115, 186, 319
AluI AGCT 4 cut(s) 109, 115, 186, 319
Alw26I GTCTC 3 cut(s) 77, 229, 437
AlwI GGATC 1 cut(s) 393
ApeKI GCWGC 4 cut(s) 80, 319, 361, 407
AsuHPI GGTGA 2 cut(s) 7, 500
BanII GRGCYC 2 cut(s) 264, 498
BbvI GCAGC 4 cut(s) 67, 306, 348, 394
BcoDI GTCTC 3 cut(s) 77, 229, 437
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 4 cut(s) 81, 320, 362, 408
BlsI GCNGC 4 cut(s) 82, 321, 363, 409
BmsI GCATC 2 cut(s) 67, 343
BpuEI CTTGAG 1 cut(s) 95
BsaI GGTCTC 2 cut(s) 77, 437
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 461, 518
Bse3DI GCAATG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 4 cut(s) 205, 319, 358, 462
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 289
BseNI ACTGG 2 cut(s) 461, 518
BseXI GCAGC 4 cut(s) 67, 306, 348, 394
BsgI GTGCAG 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 77, 229, 437
BsmI GAATGC 1 cut(s) 409
Bso31I GGTCTC 2 cut(s) 77, 437
Bsp1286I GDGCHC 2 cut(s) 264, 498
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 3 cut(s) 286, 385, 414
BspACI CCGC 2 cut(s) 83, 325
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 1 cut(s) 393
BspTNI GGTCTC 2 cut(s) 77, 437
BsrDI GCAATG 1 cut(s) 289
BsrGI TGTACA 1 cut(s) 102
BsrI ACTGG 2 cut(s) 461, 518
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 286, 385, 414
Bst4CI ACNGT 3 cut(s) 166, 193, 296
Bst6I CTCTTC 1 cut(s) 447
BstAUI TGTACA 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 4 cut(s) 205, 319, 358, 462
BstKTI GATC 3 cut(s) 289, 388, 417
BstMAI GTCTC 3 cut(s) 77, 229, 437
BstMBI GATC 3 cut(s) 286, 385, 414
BstMWI GCNNNNNNNGC 2 cut(s) 404, 539
BstNSI RCATGY 2 cut(s) 331, 477
BstSFI CTRYAG 1 cut(s) 408
BstV1I GCAGC 4 cut(s) 67, 306, 348, 394
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 4 cut(s) 205, 319, 358, 462
BtsIMutI CAGTG 2 cut(s) 417, 454
Csp6I GTAC 4 cut(s) 103, 172, 460, 471
CviAII CATG 2 cut(s) 328, 474
CviQI GTAC 4 cut(s) 103, 172, 460, 471
DpnI GATC 3 cut(s) 288, 387, 416
DpnII GATC 3 cut(s) 286, 385, 414
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco24I GRGCYC 2 cut(s) 264, 498
Eco31I GGTCTC 2 cut(s) 77, 437
EcoT38I GRGCYC 2 cut(s) 264, 498
FaeI CATG 2 cut(s) 331, 477
FaiI YATR 6 cut(s) 65, 232, 329, 401, 475, 501
FatI CATG 2 cut(s) 327, 473
FauI CCCGC 1 cut(s) 76
Fnu4HI GCNGC 4 cut(s) 81, 320, 362, 408
FokI GGATG 4 cut(s) 212, 326, 365, 449
FriOI GRGCYC 2 cut(s) 264, 498
Fsp4HI GCNGC 4 cut(s) 81, 320, 362, 408
FspBI CTAG 1 cut(s) 309
GluI GCNGC 4 cut(s) 81, 320, 362, 408
Hin1II CATG 2 cut(s) 331, 477
HincII GTYRAC 1 cut(s) 449
HindII GTYRAC 1 cut(s) 449
HinfI GANTC 3 cut(s) 57, 150, 220
HphI GGTGA 2 cut(s) 7, 500
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 119, 149
Hpy188III TCNNGA 5 cut(s) 74, 212, 290, 466, 509
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 40, 485
HpyCH4III ACNGT 3 cut(s) 166, 193, 296
HpyCH4V TGCA 2 cut(s) 322, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 539
Hsp92II CATG 2 cut(s) 331, 477
Kzo9I GATC 3 cut(s) 286, 385, 414
LpnPI CCDG 6 cut(s) 197, 368, 442, 471, 479, 499
Lsp1109I GCAGC 4 cut(s) 67, 306, 348, 394
LweI GCATC 2 cut(s) 67, 343
MaeI CTAG 1 cut(s) 309
MaeIII GTNAC 2 cut(s) 127, 290
MalI GATC 3 cut(s) 288, 387, 416
MboI GATC 3 cut(s) 286, 385, 414
MboII GAAGA 2 cut(s) 380, 434
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 264, 498
MnlI CCTC 4 cut(s) 298, 331, 342, 496
MseI TTAA 1 cut(s) 544
MslI CAYNNNNRTG 1 cut(s) 269
Mva1269I GAATGC 1 cut(s) 409
MwoI GCNNNNNNNGC 2 cut(s) 404, 539
NdeII GATC 3 cut(s) 286, 385, 414
NlaIII CATG 2 cut(s) 331, 477
NmuCI GTSAC 2 cut(s) 127, 290
NspI RCATGY 2 cut(s) 331, 477
PciI ACATGT 1 cut(s) 473
PctI GAATGC 1 cut(s) 409
PfeI GAWTC 3 cut(s) 57, 150, 220
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 4 cut(s) 82, 321, 363, 409
PscI ACATGT 1 cut(s) 473
PstI CTGCAG 1 cut(s) 412
PsuI RGATCY 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 296
RsaI GTAC 4 cut(s) 104, 173, 461, 472
RsaNI GTAC 4 cut(s) 103, 172, 460, 471
RseI CAYNNNNRTG 1 cut(s) 269
SaqAI TTAA 1 cut(s) 544
SatI GCNGC 4 cut(s) 81, 320, 362, 408
Sau3AI GATC 3 cut(s) 286, 385, 414
SduI GDGCHC 2 cut(s) 264, 498
SetI ASST 5 cut(s) 111, 117, 188, 229, 321
SfaNI GCATC 2 cut(s) 67, 343
SfcI CTRYAG 1 cut(s) 408
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SsiI CCGC 2 cut(s) 83, 325
SspMI CTAG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 166, 193, 296
TaqI TCGA 2 cut(s) 75, 223
TaqII GACCGA 1 cut(s) 59
TatI WGTACW 3 cut(s) 102, 171, 470
TfiI GAWTC 3 cut(s) 57, 150, 220
Tru1I TTAA 1 cut(s) 544
Tru9I TTAA 1 cut(s) 544
TscAI CASTG 2 cut(s) 424, 461
TseFI GTSAC 2 cut(s) 127, 290
TseI GCWGC 4 cut(s) 80, 319, 361, 407
Tsp45I GTSAC 2 cut(s) 127, 290
TspDTI ATGAA 1 cut(s) 416
TspRI CASTG 2 cut(s) 424, 461
Tth111I GACNNNGTC 1 cut(s) 296
XceI RCATGY 2 cut(s) 331, 477
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.