Rorug03G0022900
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011642
Physical Location & Seq
Reverse (-)
1824672 .. 1830856
6185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0022900.1

Sequence Viewer

Length: 1095 bp
ATGGAACCAAACGAACACCAACTCAGCTCCTACTTTAACCCCTCCACCACCACCGCCGCCGCCGCCTTGTCTCCGACCAATGGCCTCGCCGGCGGCCCAAACACCCACCAACAACAGCTCTCCTCCGCCGACAACAACAACAACAACATGGTCTACACTCCTCACTCCTCCTCCGCCGCCGCCGTGCCTCCGCCCTCGTCAGCCGTGACGTCACCGCTGGAGCCGGCGAAGAGGAAGCGCGGGAGGCCCAGGAAGTACGGCACGCCCGAGCAGGCCTTGGCGGCCAAGAAGGCCGCGACGACGTCGTCTCACTCCTCGTCTTCTAAGGAGAAGAAGGAGCAGCACCAGCACCAGCAGCAACACGGCGGCGGCTCAGCCTCGCCGTCTTATTCTGGGTCCACCAAGAAATCTCAGCTCTCTGCTCTTGGCAATTCAGGGCAAGGTTTTACACCACATGTTCTAACTGTGGCTGCAGGAGAGGATGTGGGCCAGAAAATCATGGTTTTTATGCAACAGAATAAGCGTGACATATGCATTCTGTCCGCTTCTGGTTCAATCTCAAATGCATCTATTCGCCAGCCAGCTACTTCAGGAGGCAACATTACATATGAGGGTCGCTTTGAGATCATTTCACTGTCTGGATCTTATGTAACTGACCAAGCTGGAAAGACTGGTGGGCTCAGTGTGTGTCTCTCCAATACAAATGGGCAAATCATTGGGGGTGGAGTTGGTGGACCACTGAAGGCTGCAGGCCCTGTTCAGGTTATTGTTGGTACTTTTCTGATTGATAACAAAAAGGAAAAAGTCGAGGCTTCTATGCCCAGGATGCCATCGCCAGGTGGTGGAGCATCTATGATGAATGTAGGTTTCCGCTCAACGGTGGACTCTTCTGGAAGAAATATGGTCAGGGCAAATGATGAACAACAGCAAGCTATTGGCGGAAGTCATTTCATGATCCAGGGCATGCATGGATCCACAGATTGGAGGACTGGTCCAGATGCCAGAAGCACTGGTGCTTATGAATTGGCAGGAAGAGGTGGTCGTGCAGCCCACCAATCTCCAGAAAATGGGGACTATGATCAAATTCCAGATTAG

Protein Analysis

364

Amino Acids

37.79

Weight (kDa)

9.24

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 150 - 260 3e-24 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 304
AatII GACGTC 2 cut(s) 212, 305
AccB7I CCANNNNNTGG 3 cut(s) 842, 981, 1067
AccBSI CCGCTC 1 cut(s) 873
AccI GTMKAC 1 cut(s) 153
AccII CGCG 2 cut(s) 240, 296
AclWI GGATC 4 cut(s) 649, 949, 966, 979
AcoI YGGCCR 1 cut(s) 282
AcsI RAATTY 1 cut(s) 1083
AcuI CTGAAG 2 cut(s) 573, 761
AcyI GRCGYC 2 cut(s) 209, 302
AfaI GTAC 2 cut(s) 257, 775
AfiI CCNNNNNNNGG 7 cut(s) 80, 760, 836, 842, 877, 981, 1067
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 1 cut(s) 555
AjnI CCWGG 4 cut(s) 248, 821, 835, 957
AluBI AGCT 6 cut(s) 27, 118, 415, 584, 662, 932
AluI AGCT 6 cut(s) 27, 118, 415, 584, 662, 932
Alw26I GTCTC 3 cut(s) 75, 312, 695
AlwI GGATC 4 cut(s) 649, 949, 966, 979
AlwNI CAGNNNCTG 1 cut(s) 755
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 8 cut(s) 82, 94, 245, 273, 282, 291, 487, 751
ApeKI GCWGC 5 cut(s) 340, 355, 470, 746, 1046
ApoI RAATTY 1 cut(s) 1083
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 7 cut(s) 95, 246, 396, 487, 734, 752, 992
AsuHPI GGTGA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 3 cut(s) 396, 734, 992
BamHI GGATCC 1 cut(s) 971
BanII GRGCYC 1 cut(s) 681
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 352, 367, 457, 733, 1058
BccI CCATC 1 cut(s) 838
BceAI ACGGC 5 cut(s) 167, 188, 274, 367, 379
BciT130I CCWGG 4 cut(s) 250, 823, 837, 959
BclI TGATCA 1 cut(s) 1078
BcoDI GTCTC 3 cut(s) 75, 312, 695
BfmI CTRYAG 2 cut(s) 471, 747
BglI GCCNNNNNGGC 3 cut(s) 90, 281, 290
BlpI GCTNAGC 1 cut(s) 373
Bme1390I CCNGG 4 cut(s) 250, 823, 837, 959
Bme18I GGWCC 3 cut(s) 396, 734, 992
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 7 cut(s) 95, 246, 396, 487, 734, 752, 992
BmiI GGNNCC 4 cut(s) 6, 222, 397, 973
BmrFI CCNGG 4 cut(s) 250, 823, 837, 959
BmsI GCATC 4 cut(s) 575, 816, 857, 988
BpiI GAAGAC 1 cut(s) 312
BpmI CTGGAG 2 cut(s) 239, 1044
Bpu1102I GCTNAGC 1 cut(s) 373
BsaHI GRCGYC 2 cut(s) 209, 302
BsaJI CCNNGG 4 cut(s) 248, 276, 821, 958
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 468, 498, 976, 1006
Bsc4I CCNNNNNNNGG 7 cut(s) 80, 760, 836, 842, 877, 981, 1067
Bse118I RCCGGY 2 cut(s) 89, 223
Bse1I ACTGG 3 cut(s) 676, 994, 1015
BseBI CCWGG 4 cut(s) 250, 823, 837, 959
BseDI CCNNGG 4 cut(s) 248, 276, 821, 958
BseGI GGATG 2 cut(s) 487, 831
BseLI CCNNNNNNNGG 7 cut(s) 80, 760, 836, 842, 877, 981, 1067
BseMII CTCAG 4 cut(s) 37, 387, 425, 694
BseNI ACTGG 3 cut(s) 676, 994, 1015
BseRI GAGGAG 5 cut(s) 112, 150, 157, 160, 304
BseXI GCAGC 5 cut(s) 352, 367, 457, 733, 1058
BsgI GTGCAG 1 cut(s) 1065
Bsh1236I CGCG 2 cut(s) 240, 296
BshFI GGCC 8 cut(s) 84, 96, 247, 275, 284, 293, 489, 753
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 2 cut(s) 90, 224
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 7 cut(s) 80, 760, 836, 842, 877, 981, 1067
BsmAI GTCTC 3 cut(s) 75, 312, 695
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 1085
BsmI GAATGC 1 cut(s) 534
BsnI GGCC 8 cut(s) 84, 96, 247, 275, 284, 293, 489, 753
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 681
Bsp143I GATC 5 cut(s) 624, 641, 954, 971, 1078
Bsp1720I GCTNAGC 1 cut(s) 373
BspANI GGCC 8 cut(s) 84, 96, 247, 275, 284, 293, 489, 753
BspCNI CTCAG 4 cut(s) 36, 386, 424, 693
BspFNI CGCG 2 cut(s) 240, 296
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 4 cut(s) 6, 222, 397, 973
BspMAI CTGCAG 2 cut(s) 475, 751
BspPI GGATC 4 cut(s) 649, 949, 966, 979
BsrBI CCGCTC 1 cut(s) 873
BsrFI RCCGGY 2 cut(s) 89, 223
BsrI ACTGG 3 cut(s) 676, 994, 1015
BssAI RCCGGY 2 cut(s) 89, 223
BssECI CCNNGG 4 cut(s) 248, 276, 821, 958
BssMI GATC 5 cut(s) 624, 641, 954, 971, 1078
BssNI GRCGYC 2 cut(s) 209, 302
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 4 cut(s) 250, 823, 837, 959
Bst4CI ACNGT 3 cut(s) 466, 636, 880
Bst6I CTCTTC 3 cut(s) 224, 892, 1027
BstACI GRCGYC 2 cut(s) 209, 302
BstC8I GCNNGC 9 cut(s) 91, 225, 263, 273, 578, 582, 751, 930, 965
BstDEI CTNAG 5 cut(s) 23, 324, 373, 411, 680
BstF5I GGATG 2 cut(s) 487, 831
BstFNI CGCG 2 cut(s) 240, 296
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 5 cut(s) 627, 644, 957, 974, 1081
BstMAI GTCTC 3 cut(s) 75, 312, 695
BstMBI GATC 5 cut(s) 624, 641, 954, 971, 1078
BstMWI GCNNNNNNNGC 8 cut(s) 62, 90, 244, 281, 290, 346, 355, 826
BstNI CCWGG 4 cut(s) 250, 823, 837, 959
BstNSI RCATGY 2 cut(s) 458, 967
BstSCI CCNGG 4 cut(s) 248, 821, 835, 957
BstSFI CTRYAG 2 cut(s) 471, 747
BstUI CGCG 2 cut(s) 240, 296
BstV1I GCAGC 5 cut(s) 352, 367, 457, 733, 1058
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 2 cut(s) 641, 971
BstYI RGATCY 2 cut(s) 641, 971
BsuRI GGCC 8 cut(s) 84, 96, 247, 275, 284, 293, 489, 753
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 2 cut(s) 487, 831
BtsIMutI CAGTG 4 cut(s) 632, 688, 737, 1008
Cac8I GCNNGC 9 cut(s) 91, 225, 263, 273, 578, 582, 751, 930, 965
CaiI CAGNNNCTG 1 cut(s) 755
CciI TCATGA 1 cut(s) 951
CfoI GCGC 1 cut(s) 240
Cfr10I RCCGGY 2 cut(s) 89, 223
Cfr13I GGNCC 7 cut(s) 95, 246, 396, 487, 734, 752, 992
Csp6I GTAC 2 cut(s) 256, 774
CviAII CATG 6 cut(s) 148, 455, 499, 952, 964, 968
CviQI GTAC 2 cut(s) 256, 774
DdeI CTNAG 5 cut(s) 23, 324, 373, 411, 680
DpnI GATC 5 cut(s) 626, 643, 956, 973, 1080
DpnII GATC 5 cut(s) 624, 641, 954, 971, 1078
DrdI GACNNNNNNGTC 1 cut(s) 304
DseDI GACNNNNNNGTC 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 3 cut(s) 224, 892, 1027
EarI CTCTTC 3 cut(s) 224, 892, 1027
EciI GGCGGA 4 cut(s) 115, 163, 180, 954
Eco130I CCWWGG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 681
Eco47I GGWCC 3 cut(s) 396, 734, 992
Eco57I CTGAAG 2 cut(s) 573, 761
Eco88I CYCGRG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 752
EcoRII CCWGG 4 cut(s) 248, 821, 835, 957
EcoT14I CCWWGG 1 cut(s) 276
EcoT22I ATGCAT 3 cut(s) 536, 568, 969
EcoT38I GRGCYC 1 cut(s) 681
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 6 cut(s) 151, 458, 502, 955, 967, 971
FaqI GGGAC 1 cut(s) 1085
FatI CATG 6 cut(s) 147, 454, 498, 951, 963, 967
FauI CCCGC 1 cut(s) 233
FauNDI CATATG 2 cut(s) 530, 607
FbaI TGATCA 1 cut(s) 1078
FblI GTMKAC 1 cut(s) 153
FokI GGATG 2 cut(s) 494, 838
FriOI GRGCYC 1 cut(s) 681
GlaI GCGC 1 cut(s) 239
GsuI CTGGAG 2 cut(s) 239, 1044
HaeIII GGCC 8 cut(s) 84, 96, 247, 275, 284, 293, 489, 753
HapII CCGG 2 cut(s) 90, 224
HhaI GCGC 1 cut(s) 240
Hin1I GRCGYC 2 cut(s) 209, 302
Hin1II CATG 6 cut(s) 151, 458, 502, 955, 967, 971
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HinfI GANTC 1 cut(s) 884
HpaII CCGG 2 cut(s) 90, 224
HphI GGTGA 1 cut(s) 204
Hpy166II GTNNAC 4 cut(s) 154, 399, 734, 883
Hpy188I TCNGA 2 cut(s) 75, 783
Hpy188III TCNNGA 7 cut(s) 591, 639, 891, 952, 995, 1061, 1088
Hpy8I GTNNAC 4 cut(s) 154, 399, 734, 883
Hpy99I CGWCG 3 cut(s) 301, 304, 307
HpyAV CCTTC 3 cut(s) 283, 328, 736
HpyCH4III ACNGT 3 cut(s) 466, 636, 880
HpyCH4IV ACGT 2 cut(s) 209, 302
HpyCH4V TGCA 7 cut(s) 473, 511, 534, 566, 749, 967, 1046
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 90, 244, 281, 290, 346, 355, 826
HpyF3I CTNAG 5 cut(s) 23, 324, 373, 411, 680
HpySE526I ACGT 2 cut(s) 209, 302
Hsp92I GRCGYC 2 cut(s) 209, 302
Hsp92II CATG 6 cut(s) 151, 458, 502, 955, 967, 971
HspAI GCGC 1 cut(s) 238
KroI GCCGGC 2 cut(s) 89, 223
KroNI GCCGGC 2 cut(s) 91, 225
Ksp22I TGATCA 1 cut(s) 1078
Kzo9I GATC 5 cut(s) 624, 641, 954, 971, 1078
LmnI GCTCC 4 cut(s) 32, 220, 337, 845
Lsp1109I GCAGC 5 cut(s) 352, 367, 457, 733, 1058
LweI GCATC 4 cut(s) 575, 816, 857, 988
MaeII ACGT 2 cut(s) 209, 302
MaeIII GTNAC 4 cut(s) 205, 210, 524, 649
MalI GATC 5 cut(s) 626, 643, 956, 973, 1080
MbiI CCGCTC 1 cut(s) 873
MboI GATC 5 cut(s) 624, 641, 954, 971, 1078
MboII GAAGA 6 cut(s) 241, 312, 343, 879, 906, 1044
MflI RGATCY 2 cut(s) 641, 971
MhlI GDGCHC 1 cut(s) 681
MluCI AATT 3 cut(s) 430, 1022, 1083
MlyI GAGTC 1 cut(s) 878
MmeI TCCRAC 1 cut(s) 98
Mph1103I ATGCAT 3 cut(s) 536, 568, 969
MreI CGCCGGCG 1 cut(s) 89
MroNI GCCGGC 2 cut(s) 89, 223
MseI TTAA 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 2 cut(s) 90, 224
MspR9I CCNGG 4 cut(s) 250, 823, 837, 959
Mva1269I GAATGC 1 cut(s) 534
MvaI CCWGG 4 cut(s) 250, 823, 837, 959
MvnI CGCG 2 cut(s) 240, 296
MwoI GCNNNNNNNGC 8 cut(s) 62, 90, 244, 281, 290, 346, 355, 826
NaeI GCCGGC 2 cut(s) 91, 225
NdeI CATATG 2 cut(s) 530, 607
NdeII GATC 5 cut(s) 624, 641, 954, 971, 1078
NgoMIV GCCGGC 2 cut(s) 89, 223
NlaIII CATG 6 cut(s) 151, 458, 502, 955, 967, 971
NlaIV GGNNCC 4 cut(s) 6, 222, 397, 973
NmuCI GTSAC 3 cut(s) 205, 210, 524
NsiI ATGCAT 3 cut(s) 536, 568, 969
NspI RCATGY 2 cut(s) 458, 967
PaeI GCATGC 1 cut(s) 967
PagI TCATGA 1 cut(s) 951
PceI AGGCCT 1 cut(s) 275
PciI ACATGT 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 534
PdiI GCCGGC 2 cut(s) 91, 225
PflFI GACNNNGTC 2 cut(s) 301, 304
PflMI CCANNNNNTGG 3 cut(s) 842, 981, 1067
PleI GAGTC 1 cut(s) 878
PpsI GAGTC 1 cut(s) 878
PscI ACATGT 1 cut(s) 454
Psp6I CCWGG 4 cut(s) 248, 821, 835, 957
PspGI CCWGG 4 cut(s) 248, 821, 835, 957
PspN4I GGNNCC 4 cut(s) 6, 222, 397, 973
PspPI GGNCC 7 cut(s) 95, 246, 396, 487, 734, 752, 992
PstI CTGCAG 2 cut(s) 475, 751
PstNI CAGNNNCTG 1 cut(s) 755
PsuI RGATCY 2 cut(s) 641, 971
PsyI GACNNNGTC 2 cut(s) 301, 304
RsaI GTAC 2 cut(s) 257, 775
RsaNI GTAC 2 cut(s) 256, 774
SaqAI TTAA 1 cut(s) 36
Sau3AI GATC 5 cut(s) 624, 641, 954, 971, 1078
Sau96I GGNCC 7 cut(s) 95, 246, 396, 487, 734, 752, 992
SchI GAGTC 1 cut(s) 878
ScrFI CCNGG 4 cut(s) 250, 823, 837, 959
SduI GDGCHC 1 cut(s) 681
SfaNI GCATC 4 cut(s) 575, 816, 857, 988
SfcI CTRYAG 2 cut(s) 471, 747
SfiI GGCCNNNNNGGCC 2 cut(s) 281, 290
SgrAI CRCCGGYG 1 cut(s) 89
SinI GGWCC 3 cut(s) 396, 734, 992
SphI GCATGC 1 cut(s) 967
Sse9I AATT 3 cut(s) 430, 1022, 1083
SseBI AGGCCT 1 cut(s) 275
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 4 cut(s) 248, 821, 835, 957
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 466, 636, 880
TaiI ACGT 2 cut(s) 212, 305
TaqI TCGA 1 cut(s) 807
TasI AATT 3 cut(s) 430, 1022, 1083
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 4 cut(s) 639, 688, 744, 1015
TseFI GTSAC 3 cut(s) 205, 210, 524
TseI GCWGC 5 cut(s) 340, 355, 470, 746, 1046
Tsp45I GTSAC 3 cut(s) 205, 210, 524
TspDTI ATGAA 4 cut(s) 872, 933, 940, 1035
TspRI CASTG 4 cut(s) 639, 688, 744, 1015
Tth111I GACNNNGTC 2 cut(s) 301, 304
Van91I CCANNNNNTGG 3 cut(s) 842, 981, 1067
VpaK11BI GGWCC 3 cut(s) 396, 734, 992
XapI RAATTY 1 cut(s) 1083
XceI RCATGY 2 cut(s) 458, 967
XcmI CCANNNNNNNNNTGG 1 cut(s) 965
XmiI GTMKAC 1 cut(s) 153
ZraI GACGTC 2 cut(s) 210, 303
Zsp2I ATGCAT 3 cut(s) 536, 568, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.