RLG00000025191
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011642
Physical Location & Seq
Forward (+)
42915311 .. 42918237
2927 bp
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UTR
Exon/CDS
Intron
RLM00000025191

Sequence Viewer

Length: 693 bp
ATGCACCAAACGTCGTTAAATCCAGTCGTTATCTCGGACCCGTATTTATATTCCCAAAACAGACTCTTCAGAGCCCATTTCATCGCGTCCCCGTCATTTCTCTCTAGGGCAGCTTTTCAGATCTGCCCCCTCCAAGTGAATTCGAAGATGGGGTTGTCTTTCACCAAGTTGTTCAGTCGGCTTTTTGCCAAGAAGGAAATGAGAATCCTTATGGTCGGTCTCGATGCTGCGGGTAAAACTACCATTTTGTACAAGCTCAAGCTCGGAGAGATTGTGACCACCATTCCTACTATCGGATTCAATGTTGAAACCGTGGAGTACAAGAACATTAGCTTTACCGTGTGGGATGTCGGGGGTCAGGACAAGATTCGACCTTTATGGAGACACTACTTCCAAAATACACAAGGGCTCATTTTTGTGGTTGATAGCAATGATCGTGACCGCGTCGTTGAGGCTAGGGATGAGCTGCACCGCATGTTGAATGAGGACGAGTTGAGGGATGCTGTGCTGCTTGTATTTGCCAACAAGCAGGATCTTCCAAATGCTATGAATGCTGCAGAGATCACTGACAAACTTGGTCTCCACTCACTTCGTCAACGCCACTGGTACATCCAGAGTACATGTGCCACTTCTGGTGAAGGGCTGTATGAGGGACTTGACTGGCTCTCAAACAATATCGCAAACAAGCCCTAA

Protein Analysis

231

Amino Acids

26.28

Weight (kDa)

8.49

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 54 - 226 2.9e-81 ADP-ribosylation factor family
SRPRB PF09439 65 - 192 9.7e-13 Signal recognition particle receptor beta subunit
Roc PF08477 68 - 178 1.6e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 68 - 222 1.1e-11 Ras family
Gtr1_RagA PF04670 68 - 190 3.3e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 94 - 160 1.3e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 86, 444
AciI CCGC 3 cut(s) 230, 442, 472
AclWI GGATC 1 cut(s) 540
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 52
AfaI GTAC 4 cut(s) 251, 320, 608, 619
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 620
AgsI TTSAA 3 cut(s) 301, 308, 481
AluBI AGCT 5 cut(s) 113, 256, 262, 333, 466
AluI AGCT 5 cut(s) 113, 256, 262, 333, 466
Alw26I GTCTC 3 cut(s) 224, 376, 584
AlwI GGATC 1 cut(s) 540
ApeKI GCWGC 5 cut(s) 110, 227, 466, 508, 554
ApoI RAATTY 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 154, 647
AsuII TTCGAA 1 cut(s) 143
AvaII GGWCC 1 cut(s) 37
BanII GRGCYC 2 cut(s) 76, 411
BbvI GCAGC 5 cut(s) 122, 214, 453, 495, 541
BccI CCATC 1 cut(s) 142
BcoDI GTCTC 3 cut(s) 224, 376, 584
BfaI CTAG 2 cut(s) 105, 456
BfmI CTRYAG 1 cut(s) 555
BglII AGATCT 1 cut(s) 120
BisI GCNGC 5 cut(s) 111, 228, 467, 509, 555
BlsI GCNGC 5 cut(s) 112, 229, 468, 510, 556
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 39
BmsI GCATC 2 cut(s) 214, 490
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 242
BsaI GGTCTC 2 cut(s) 224, 584
BsaJI CCNNGG 1 cut(s) 312
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 3 cut(s) 23, 608, 665
Bse3DI GCAATG 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 4 cut(s) 352, 466, 505, 609
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 436
BseNI ACTGG 3 cut(s) 23, 608, 665
BseXI GCAGC 5 cut(s) 122, 214, 453, 495, 541
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 2 cut(s) 86, 444
BslFI GGGAC 2 cut(s) 73, 666
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 3 cut(s) 224, 376, 584
BsmFI GGGAC 2 cut(s) 73, 666
BsmI GAATGC 1 cut(s) 556
Bso31I GGTCTC 2 cut(s) 224, 584
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 76, 411
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 4 cut(s) 120, 433, 532, 561
BspACI CCGC 3 cut(s) 230, 442, 472
BspFNI CGCG 2 cut(s) 86, 444
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 1 cut(s) 540
BspT104I TTCGAA 1 cut(s) 143
BspTNI GGTCTC 2 cut(s) 224, 584
BsrDI GCAATG 1 cut(s) 436
BsrGI TGTACA 1 cut(s) 249
BsrI ACTGG 3 cut(s) 23, 608, 665
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 4 cut(s) 120, 433, 532, 561
Bst4CI ACNGT 2 cut(s) 313, 340
Bst6I CTCTTC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 249
BstBI TTCGAA 1 cut(s) 143
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 352, 466, 505, 609
BstFNI CGCG 2 cut(s) 86, 444
BstKTI GATC 4 cut(s) 123, 436, 535, 564
BstMAI GTCTC 3 cut(s) 224, 376, 584
BstMBI GATC 4 cut(s) 120, 433, 532, 561
BstMWI GCNNNNNNNGC 1 cut(s) 551
BstNSI RCATGY 2 cut(s) 478, 624
BstSFI CTRYAG 1 cut(s) 555
BstUI CGCG 2 cut(s) 86, 444
BstV1I GCAGC 5 cut(s) 122, 214, 453, 495, 541
BstX2I RGATCY 2 cut(s) 120, 532
BstYI RGATCY 2 cut(s) 120, 532
BtgI CCRYGG 1 cut(s) 312
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 352, 466, 505, 609
BtsIMutI CAGTG 2 cut(s) 564, 601
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 75, 433
Csp6I GTAC 4 cut(s) 250, 319, 607, 618
CviAII CATG 2 cut(s) 475, 621
CviQI GTAC 4 cut(s) 250, 319, 607, 618
DpnI GATC 4 cut(s) 122, 435, 534, 563
DpnII GATC 4 cut(s) 120, 433, 532, 561
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco24I GRGCYC 2 cut(s) 76, 411
Eco31I GGTCTC 2 cut(s) 224, 584
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 139
EcoT38I GRGCYC 2 cut(s) 76, 411
FaeI CATG 2 cut(s) 478, 624
FaiI YATR 7 cut(s) 49, 212, 379, 476, 548, 622, 648
FaqI GGGAC 2 cut(s) 73, 666
FatI CATG 2 cut(s) 474, 620
FauI CCCGC 1 cut(s) 223
Fnu4HI GCNGC 5 cut(s) 111, 228, 467, 509, 555
FokI GGATG 4 cut(s) 359, 473, 512, 596
FriOI GRGCYC 2 cut(s) 76, 411
Fsp4HI GCNGC 5 cut(s) 111, 228, 467, 509, 555
FspBI CTAG 2 cut(s) 105, 456
GluI GCNGC 5 cut(s) 111, 228, 467, 509, 555
HgaI GACGC 2 cut(s) 75, 433
Hin1II CATG 2 cut(s) 478, 624
HincII GTYRAC 1 cut(s) 596
HindII GTYRAC 1 cut(s) 596
HinfI GANTC 4 cut(s) 63, 204, 297, 367
HphI GGTGA 2 cut(s) 154, 647
Hpy166II GTNNAC 1 cut(s) 596
Hpy188I TCNGA 5 cut(s) 37, 71, 120, 266, 296
Hpy188III TCNNGA 4 cut(s) 221, 359, 437, 613
Hpy8I GTNNAC 1 cut(s) 596
Hpy99I CGWCG 2 cut(s) 16, 449
HpyAV CCTTC 2 cut(s) 187, 632
HpyCH4III ACNGT 2 cut(s) 313, 340
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 4, 469, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 551
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 2 cut(s) 478, 624
Kzo9I GATC 4 cut(s) 120, 433, 532, 561
LpnPI CCDG 7 cut(s) 36, 344, 515, 589, 618, 626, 646
Lsp1109I GCAGC 5 cut(s) 122, 214, 453, 495, 541
LweI GCATC 2 cut(s) 214, 490
MaeI CTAG 2 cut(s) 105, 456
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 2 cut(s) 274, 437
MalI GATC 4 cut(s) 122, 435, 534, 563
MboI GATC 4 cut(s) 120, 433, 532, 561
MboII GAAGA 3 cut(s) 58, 157, 527
MflI RGATCY 2 cut(s) 120, 532
MhlI GDGCHC 2 cut(s) 76, 411
MluCI AATT 1 cut(s) 139
MlyI GAGTC 1 cut(s) 57
MnlI CCTC 5 cut(s) 140, 445, 478, 489, 643
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 416
Mva1269I GAATGC 1 cut(s) 556
MvnI CGCG 2 cut(s) 86, 444
MwoI GCNNNNNNNGC 1 cut(s) 551
NdeII GATC 4 cut(s) 120, 433, 532, 561
NlaIII CATG 2 cut(s) 478, 624
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 274, 437
NspI RCATGY 2 cut(s) 478, 624
NspV TTCGAA 1 cut(s) 143
PciI ACATGT 1 cut(s) 620
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 204, 297, 367
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 5 cut(s) 112, 229, 468, 510, 556
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
PscI ACATGT 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 2 cut(s) 120, 532
PsyI GACNNNGTC 1 cut(s) 443
RsaI GTAC 4 cut(s) 251, 320, 608, 619
RsaNI GTAC 4 cut(s) 250, 319, 607, 618
RseI CAYNNNNRTG 1 cut(s) 416
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 5 cut(s) 111, 228, 467, 509, 555
Sau3AI GATC 4 cut(s) 120, 433, 532, 561
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 1 cut(s) 57
SduI GDGCHC 2 cut(s) 76, 411
SetI ASST 7 cut(s) 14, 115, 258, 264, 335, 376, 468
SfaNI GCATC 2 cut(s) 214, 490
SfcI CTRYAG 1 cut(s) 555
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 1 cut(s) 139
SsiI CCGC 3 cut(s) 230, 442, 472
SspMI CTAG 2 cut(s) 105, 456
TaaI ACNGT 2 cut(s) 313, 340
TaiI ACGT 1 cut(s) 14
TaqI TCGA 3 cut(s) 143, 222, 370
TaqII GACCGA 1 cut(s) 206
TasI AATT 1 cut(s) 139
TatI WGTACW 3 cut(s) 249, 318, 617
TfiI GAWTC 3 cut(s) 204, 297, 367
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 2 cut(s) 571, 608
TseFI GTSAC 2 cut(s) 274, 437
TseI GCWGC 5 cut(s) 110, 227, 466, 508, 554
Tsp45I GTSAC 2 cut(s) 274, 437
TspDTI ATGAA 2 cut(s) 70, 563
TspRI CASTG 2 cut(s) 571, 608
Tth111I GACNNNGTC 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 139
XceI RCATGY 2 cut(s) 478, 624
XspI CTAG 2 cut(s) 105, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.