RchiOBHm_Chr3g0459181

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017793
Physical Location & Seq
Reverse (-)
7685633 .. 7688732
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42577

Sequence Viewer

Length: 1440 bp
ATGTACTCTGACACAAACTTGAAAGCAACAATGGCAAGCACAAGCTCCAAACCCAAAATCAAGACTAGGATCAAGATTAACAGACCCCTTTATATCAACTTACTGTTTGACAAGGAAAAACTTCCTTCATCTCTTTGGGGAGGCGGGCCCACCATAGTCCCTGGACCAGGAGGACCGGAGGACTTGCCGGAAGTTTTCAACAAGGTGATGAACACCACCGAAGGATTCATGAAGAGTTTTGTCAGGATGCATGGTAATCTGTTAAAAGACGGCTATGAGGACTTGGCTCATGAGATTGAAACTCAGTTGACGGAAAAGGACATTGTTCCCATGATTATTGTGGTCACCGGCATCATTGAGGAGTACGCGGATGCTGGCAAGACCATGGAAGCTCTGGTGTTGTACCTGATTCTTTTGGTCTCTAATTGTGTCCCCAACCCTTACACATATAGTGTCCTCATCAAGGCACTGGCTGCAGACCCGGAACCCAAATTTCTTGAGGTTGCAAAGGAGTGCATACTGGAGATGATGGAAAAGGGATGGCAGCCAAATGCCGAGACCTACACTGCCGTGTTTAAGGCATTTGCTTTGCAGGAGAAGGTCGAAGAGGGTAAGAAGTTTCGGGAGGAAATGGAAGCCAAGGGCTTCGTGGCAGACAAGATGGCTGTGATTGATGCTCTCAAGGGGACTACAGGACTGGTGGTTAAAAGCATTCACAACATTCTCTTTGATGACTCAGAGTACTCCGACTCAGATGACGATGATGATATAAAGAAATTCTACAAACTGATCGACTTGCAGAAGCTTTCTCCTTATAGTTTTGTAACTCCTCTCCTCAAGATTCCTGATATTGAAAATGACCCAGAGAACCCCAAAGATTTTCAACAAGTCTTCAACAAGATGCACACCGCCGAAGGTTTTGTCAAAACTGTGGTAAGGATGTTCAAAATGCTAGAACGTGAAGGCTTCCTATCTCAGGCTCAAGAGATCAGGGCCAAGATATATTCTTTTTGTGAATCTAATGATATGCCCGAAGTCGTGATTCACACCGGCATGATGGAGATCTACTCCAAGGCTGACAGGTCCAAGGAGGCTCTCAGGGTGTTCCAGAGGATGATATCCCTTGGTGTTGCTCCCAACGCATACACCTATAGTGTTCTCATCAAGGGACTCACTAAAGACCCCAACTTCCTTGATGATGCCAAGCAGTATGTGCTGGAAATGATGGGGAATGGAATGCAACCCACTGCCAAGACCTACACCACTTTGTTTTATGCTTTTACCCAGCTGGGGAAGGATAAGTTGAAGGAGGCTAAGGAGTTTGTGAAGCAGATGAAAGCCAGGGGTTTTGTAGCTAACAAGAAAGCTGTGAAGGAAGTTCTGAAGGGTAAAAAAGCACCTATTGCTAAAATGGTCATCAACATTCTCTTTGCTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

479

Amino Acids

54.09

Weight (kDa)

6.13

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 114 - 158 1.4e-06 PPR repeat family
PPR_2 PF13041 344 - 393 9e-12 PPR repeat family
PPR_long PF17177 360 - 462 1.2e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017793)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0459181
rosa_laevigata RLG00000025105
rosa_multiflora Rmu_sc0002435.1_g000015 Rmu_sc0004343.1_g000004 Rmu_sc0014208.1_g000004 Rmu_sc0024269.1_g000003
rosa_roxburghii Rroxscaffold_6G00420440
rosa_rugosa Rorug03G0034100 Rorug03G0034100
rosa_samantha Rh3CG095200 Rh3CG096000
rosa_wichuraiana Rw3G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 368
AciI CCGC 3 cut(s) 144, 368, 909
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 2 cut(s) 491, 776
AcuI CTGAAG 1 cut(s) 1403
AfaI GTAC 4 cut(s) 5, 365, 404, 743
AfiI CCNNNNNNNGG 4 cut(s) 167, 463, 976, 1290
AgsI TTSAA 8 cut(s) 22, 199, 299, 854, 884, 895, 946, 1306
AjnI CCWGG 3 cut(s) 160, 166, 1340
AleI CACNNNNGTG 1 cut(s) 569
AluBI AGCT 6 cut(s) 45, 392, 805, 1288, 1355, 1367
AluI AGCT 6 cut(s) 45, 392, 805, 1288, 1355, 1367
Alw26I GTCTC 2 cut(s) 424, 551
AlwI GGATC 1 cut(s) 77
AoxI GGCC 2 cut(s) 146, 993
ApaI GGGCCC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 473, 544
ApoI RAATTY 2 cut(s) 491, 776
ArsI GACNNNNNNTTYG 2 cut(s) 906, 938
Asp700I GAANNNNTTC 1 cut(s) 120
AspS9I GGNCC 6 cut(s) 146, 147, 164, 173, 993, 1083
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 217, 337
AvaII GGWCC 3 cut(s) 164, 173, 1083
BaeGI GKGCMC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 883
BbvI GCAGC 2 cut(s) 460, 556
BccI CCATC 5 cut(s) 523, 534, 655, 1051, 1219
BceAI ACGGC 2 cut(s) 286, 554
BciT130I CCWGG 3 cut(s) 162, 168, 1342
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 2 cut(s) 424, 551
BfaI CTAG 2 cut(s) 66, 953
BfmI CTRYAG 3 cut(s) 474, 690, 1150
BglII AGATCT 1 cut(s) 1062
BisI GCNGC 2 cut(s) 474, 545
BlsI GCNGC 2 cut(s) 475, 546
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 4 cut(s) 162, 168, 482, 1342
Bme18I GGWCC 3 cut(s) 164, 173, 1083
BmgT120I GGNCC 6 cut(s) 146, 147, 164, 173, 993, 1083
BmiI GGNNCC 2 cut(s) 148, 486
BmrFI CCNGG 4 cut(s) 162, 168, 482, 1342
BmsI GCATC 6 cut(s) 237, 360, 361, 664, 891, 1189
BpiI GAAGAC 1 cut(s) 883
BplI GAGNNNNNCTC 2 cut(s) 1052, 1084
BpmI CTGGAG 1 cut(s) 542
Bpu10I CCTNAGC 1 cut(s) 1314
BpuEI CTTGAG 4 cut(s) 518, 665, 821, 966
BpuMI CCSGG 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 1061
BsaI GGTCTC 2 cut(s) 424, 551
BsaJI CCNNGG 7 cut(s) 160, 384, 639, 1071, 1086, 1123, 1341
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 463, 976, 1290
Bse118I RCCGGY 2 cut(s) 347, 1049
Bse1I ACTGG 3 cut(s) 474, 525, 702
Bse8I GATNNNNATC 1 cut(s) 1061
BseBI CCWGG 3 cut(s) 162, 168, 1342
BseDI CCNNGG 7 cut(s) 160, 384, 639, 1071, 1086, 1123, 1341
BseGI GGATG 5 cut(s) 252, 376, 545, 945, 1119
BseJI GATNNNNATC 1 cut(s) 1061
BseLI CCNNNNNNNGG 4 cut(s) 167, 463, 976, 1290
BseMII CTCAG 6 cut(s) 317, 750, 765, 989, 1111, 1425
BseNI ACTGG 3 cut(s) 474, 525, 702
BseRI GAGGAG 3 cut(s) 374, 819, 824
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 2 cut(s) 460, 556
BseYI CCCAGC 2 cut(s) 1284, 1288
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 2 cut(s) 148, 995
BsiSI CCGG 5 cut(s) 176, 188, 348, 482, 1050
BslFI GGGAC 4 cut(s) 143, 416, 700, 1182
BslI CCNNNNNNNGG 4 cut(s) 167, 463, 976, 1290
BsmAI GTCTC 2 cut(s) 424, 551
BsmFI GGGAC 4 cut(s) 143, 416, 700, 1182
BsmI GAATGC 2 cut(s) 711, 1242
BsnI GGCC 2 cut(s) 148, 995
Bso31I GGTCTC 2 cut(s) 424, 551
Bsp120I GGGCCC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 69, 789, 987, 1062
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 144, 368, 909
BspANI GGCC 2 cut(s) 148, 995
BspCNI CTCAG 6 cut(s) 316, 749, 764, 988, 1110, 1426
BspFNI CGCG 1 cut(s) 368
BspHI TCATGA 2 cut(s) 228, 289
BspLI GGNNCC 2 cut(s) 148, 486
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 1 cut(s) 77
BspTNI GGTCTC 2 cut(s) 424, 551
BsrFI RCCGGY 2 cut(s) 347, 1049
BsrI ACTGG 3 cut(s) 474, 525, 702
BssAI RCCGGY 2 cut(s) 347, 1049
BssECI CCNNGG 7 cut(s) 160, 384, 639, 1071, 1086, 1123, 1341
BssMI GATC 4 cut(s) 69, 789, 987, 1062
BssT1I CCWWGG 5 cut(s) 384, 639, 1071, 1086, 1123
Bst2UI CCWGG 3 cut(s) 162, 168, 1342
Bst4CI ACNGT 2 cut(s) 105, 931
Bst6I CTCTTC 2 cut(s) 227, 600
BstAPI GCANNNNNTGC 3 cut(s) 473, 1213, 1403
BstC8I GCNNGC 3 cut(s) 37, 146, 376
BstDEI CTNAG 7 cut(s) 303, 736, 751, 975, 1097, 1314, 1434
BstDSI CCRYGG 1 cut(s) 384
BstEII GGTNACC 1 cut(s) 343
BstENI CCTNNNNNAGG 3 cut(s) 165, 461, 974
BstF5I GGATG 5 cut(s) 252, 376, 545, 945, 1119
BstFNI CGCG 1 cut(s) 368
BstKTI GATC 4 cut(s) 72, 792, 990, 1065
BstMAI GTCTC 2 cut(s) 424, 551
BstMBI GATC 4 cut(s) 69, 789, 987, 1062
BstMWI GCNNNNNNNGC 5 cut(s) 32, 473, 1139, 1213, 1403
BstNI CCWGG 3 cut(s) 162, 168, 1342
BstPI GGTNACC 1 cut(s) 343
BstSCI CCNGG 4 cut(s) 160, 166, 480, 1340
BstSFI CTRYAG 3 cut(s) 474, 690, 1150
BstSLI GKGCMC 1 cut(s) 150
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 2 cut(s) 460, 556
BstV2I GAAGAC 1 cut(s) 883
BstX2I RGATCY 1 cut(s) 1062
BstYI RGATCY 1 cut(s) 1062
BsuRI GGCC 2 cut(s) 148, 995
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 5 cut(s) 252, 376, 545, 945, 1119
BtsI GCAGTG 2 cut(s) 564, 1245
BtsIMutI CAGTG 3 cut(s) 467, 564, 1245
Cac8I GCNNGC 3 cut(s) 37, 146, 376
CciI TCATGA 2 cut(s) 228, 289
Cfr10I RCCGGY 2 cut(s) 347, 1049
Cfr13I GGNCC 6 cut(s) 146, 147, 164, 173, 993, 1083
Csp6I GTAC 4 cut(s) 4, 364, 403, 742
CviAII CATG 6 cut(s) 229, 251, 290, 331, 385, 1054
CviQI GTAC 4 cut(s) 4, 364, 403, 742
DdeI CTNAG 7 cut(s) 303, 736, 751, 975, 1097, 1314, 1434
DpnI GATC 4 cut(s) 71, 791, 989, 1064
DpnII GATC 4 cut(s) 69, 789, 987, 1062
Eam1104I CTCTTC 2 cut(s) 227, 600
EarI CTCTTC 2 cut(s) 227, 600
Eco130I CCWWGG 5 cut(s) 384, 639, 1071, 1086, 1123
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 2 cut(s) 424, 551
Eco32I GATATC 1 cut(s) 1119
Eco47I GGWCC 3 cut(s) 164, 173, 1083
Eco57I CTGAAG 1 cut(s) 1403
Eco91I GGTNACC 1 cut(s) 343
EcoNI CCTNNNNNAGG 3 cut(s) 165, 461, 974
EcoO65I GGTNACC 1 cut(s) 343
EcoRII CCWGG 3 cut(s) 160, 166, 1340
EcoRV GATATC 1 cut(s) 1119
EcoT14I CCWWGG 5 cut(s) 384, 639, 1071, 1086, 1123
EcoT22I ATGCAT 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 5 cut(s) 384, 639, 1071, 1086, 1123
FaeI CATG 6 cut(s) 232, 254, 293, 334, 388, 1057
FaqI GGGAC 4 cut(s) 143, 416, 700, 1182
FatI CATG 6 cut(s) 228, 250, 289, 330, 384, 1053
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 2 cut(s) 474, 545
FokI GGATG 5 cut(s) 259, 383, 552, 952, 1126
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 474, 545
FspBI CTAG 2 cut(s) 66, 953
GluI GCNGC 2 cut(s) 474, 545
GsaI CCCAGC 2 cut(s) 1288, 1292
GsuI CTGGAG 1 cut(s) 542
HaeIII GGCC 2 cut(s) 148, 995
HapII CCGG 5 cut(s) 176, 188, 348, 482, 1050
Hin1II CATG 6 cut(s) 232, 254, 293, 334, 388, 1057
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 8 cut(s) 225, 409, 734, 749, 841, 1016, 1042, 1170
HpaII CCGG 5 cut(s) 176, 188, 348, 482, 1050
HphI GGTGA 2 cut(s) 217, 337
Hpy166II GTNNAC 1 cut(s) 309
Hpy188I TCNGA 5 cut(s) 10, 739, 748, 754, 1383
Hpy8I GTNNAC 1 cut(s) 309
HpyAV CCTTC 9 cut(s) 135, 215, 592, 908, 956, 1288, 1300, 1366, 1378
HpyCH4III ACNGT 2 cut(s) 105, 931
HpyCH4IV ACGT 1 cut(s) 958
HpyCH4V TGCA 8 cut(s) 250, 476, 506, 516, 592, 799, 904, 1240
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 473, 1139, 1213, 1403
HpyF3I CTNAG 7 cut(s) 303, 736, 751, 975, 1097, 1314, 1434
HpySE526I ACGT 1 cut(s) 958
Hsp92II CATG 6 cut(s) 232, 254, 293, 334, 388, 1057
Kzo9I GATC 4 cut(s) 69, 789, 987, 1062
LmnI GCTCC 2 cut(s) 50, 1138
Lsp1109I GCAGC 2 cut(s) 460, 556
LweI GCATC 6 cut(s) 237, 360, 361, 664, 891, 1189
MaeI CTAG 2 cut(s) 66, 953
MaeII ACGT 1 cut(s) 958
MaeIII GTNAC 2 cut(s) 343, 823
MalI GATC 4 cut(s) 71, 791, 989, 1064
MboI GATC 4 cut(s) 69, 789, 987, 1062
MboII GAAGA 3 cut(s) 244, 617, 883
MflI RGATCY 1 cut(s) 1062
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 3 cut(s) 424, 491, 776
MlyI GAGTC 3 cut(s) 728, 743, 1164
MmeI TCCRAC 1 cut(s) 771
Mph1103I ATGCAT 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 4 cut(s) 78, 263, 576, 705
MslI CAYNNNNRTG 2 cut(s) 569, 1052
MspA1I CMGCKG 1 cut(s) 1288
MspI CCGG 5 cut(s) 176, 188, 348, 482, 1050
MspR9I CCNGG 4 cut(s) 162, 168, 482, 1342
Mva1269I GAATGC 2 cut(s) 711, 1242
MvaI CCWGG 3 cut(s) 162, 168, 1342
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 5 cut(s) 32, 473, 1139, 1213, 1403
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 1 cut(s) 384
NdeII GATC 4 cut(s) 69, 789, 987, 1062
NlaIII CATG 6 cut(s) 232, 254, 293, 334, 388, 1057
NlaIV GGNNCC 2 cut(s) 148, 486
NmeAIII GCCGAG 1 cut(s) 580
NmuCI GTSAC 1 cut(s) 343
NsiI ATGCAT 1 cut(s) 252
OliI CACNNNNGTG 1 cut(s) 569
PagI TCATGA 2 cut(s) 228, 289
PctI GAATGC 2 cut(s) 711, 1242
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 5 cut(s) 225, 409, 841, 1016, 1042
PkrI GCNGC 2 cut(s) 475, 546
PleI GAGTC 3 cut(s) 728, 743, 1164
PpsI GAGTC 3 cut(s) 728, 743, 1164
Psp6I CCWGG 3 cut(s) 160, 166, 1340
PspEI GGTNACC 1 cut(s) 343
PspFI CCCAGC 2 cut(s) 1284, 1288
PspGI CCWGG 3 cut(s) 160, 166, 1340
PspN4I GGNNCC 2 cut(s) 148, 486
PspOMI GGGCCC 1 cut(s) 146
PspPI GGNCC 6 cut(s) 146, 147, 164, 173, 993, 1083
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 1062
PvuII CAGCTG 1 cut(s) 1288
RsaI GTAC 4 cut(s) 5, 365, 404, 743
RsaNI GTAC 4 cut(s) 4, 364, 403, 742
RseI CAYNNNNRTG 2 cut(s) 569, 1052
SaqAI TTAA 4 cut(s) 78, 263, 576, 705
SatI GCNGC 2 cut(s) 474, 545
Sau3AI GATC 4 cut(s) 69, 789, 987, 1062
Sau96I GGNCC 6 cut(s) 146, 147, 164, 173, 993, 1083
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 3 cut(s) 728, 743, 1164
ScrFI CCNGG 4 cut(s) 162, 168, 482, 1342
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 6 cut(s) 237, 360, 361, 664, 891, 1189
SfcI CTRYAG 3 cut(s) 474, 690, 1150
SinI GGWCC 3 cut(s) 164, 173, 1083
SmiMI CAYNNNNRTG 2 cut(s) 569, 1052
SmlI CTYRAG 4 cut(s) 497, 680, 836, 981
SmoI CTYRAG 4 cut(s) 497, 680, 836, 981
Sse9I AATT 3 cut(s) 424, 491, 776
SsiI CCGC 3 cut(s) 144, 368, 909
SspMI CTAG 2 cut(s) 66, 953
StyD4I CCNGG 4 cut(s) 160, 166, 480, 1340
StyI CCWWGG 5 cut(s) 384, 639, 1071, 1086, 1123
TaaI ACNGT 2 cut(s) 105, 931
TaiI ACGT 1 cut(s) 961
TaqI TCGA 2 cut(s) 603, 792
TasI AATT 3 cut(s) 424, 491, 776
TatI WGTACW 2 cut(s) 3, 741
TfiI GAWTC 5 cut(s) 225, 409, 841, 1016, 1042
Tru1I TTAA 4 cut(s) 78, 263, 576, 705
Tru9I TTAA 4 cut(s) 78, 263, 576, 705
TscAI CASTG 3 cut(s) 474, 571, 1252
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 2 cut(s) 473, 544
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 5 cut(s) 117, 217, 224, 245, 1349
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 3 cut(s) 474, 571, 1252
VpaK11BI GGWCC 3 cut(s) 164, 173, 1083
XagI CCTNNNNNAGG 3 cut(s) 165, 461, 974
XapI RAATTY 2 cut(s) 491, 776
XcmI CCANNNNNNNNNTGG 3 cut(s) 337, 391, 646
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 2 cut(s) 66, 953
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.