Rroxscaffold_6G00420440

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017793
Physical Location & Seq
Forward (+)
41699489 .. 41702327
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420440.1

Sequence Viewer

Length: 1410 bp
ATGGCAAGCACAAGCTCCAAACCCAAAATCAAGACTAGGATCAAGATTAACAGACCCCTTTATATCAACTTACTGTTTGACAAGGAAAAACTTCCTTCATCTCTTTGGGGAGGCGGGCCCACCATAGTCCCTGGACCAGGAGGACCGGAGGACTTGCCGGAAGTTTTCAACAAGGTGATGAACACCACCGAAGGATTCATGAAGAGTTTTGTCAGGATGCACGGTAATCTGTTAAAAGACGGCTATGAGGACTTGGCTCATGAGATTGAAACTCAATTAACGGAAAAGGACAATGTTCCCATGATTATTGTGGTCACCGGCATCATTGAGGAGTACGCGGATGCCGGCAAGACCATGGAAGCTCTGGTGGTGTACCTGATTCTTTTGGTCTCTAATTGTGTCCCCAACCCTTACACATATGGTGTTCTCATCAAGGCACTGGCTGCAGACCCGGACCCCAAATTTCTTGAGGTTGCAAAGGAGTGCATACTGGAGATGATGGAAAAGGGATGGCAGCCCAATGCCGAGACCTACACTGCAGTGTTTAAGGCATTTGCTTTGCAGGAGAAGGTCGAAGAGGGTAAGAAGTTTCGGGAGGAAATGGAAGTTACGGGCTTCGTGGCAGACAAGATGGCTGTGATTAATGCTCTCAAGGGGACTACAGGACCGGTGGTTAAAAGCATTCACAACGTTCTCTTTGATGATTCAGAGTACTCCGACTCAGATGAGGATGATGATATAAAGAAATTCTACAAACTGATTGACTGGCAGAAGCTTTCTCCTTATAGTTTTGTAACTCCTCTCCTCAAGATTCCTGATATTGAAAATGACCCAGAGAACCCCAAAGATTTTCAACAAGTCTTCAACAAGATGCACACCGCAGAAGGTTTTGTCAAAACTGTGGTAAGGATGTTCAAAATGCTAGAACGTGAAGGCTTCCTATCTCAGGCTCAAGAGATCAGGGCCAAGATATATTCTCTTTGTGAATCTAATGATATGCCTGAAGTCGTGATTCACACCGGCATGATGGAGATCTACTCCAAGGCTGACAGGTCCAAGGAGGCTCTCAGGGTGTTCCAGCGGATGATATCCCTTGGTGTTGCTCCCAACGCATACACCTATAGTGTTCTCATCAAGGCACTCACTAAAGACCCCAAGTTCCTTGATGATGCCAAGCAGTATGTGCTGGAAATGATGGGGAATGGAATGCAACCCAATGCCAAGACCTACACCACTTTGTTTTATGCTTTTGCCCAGCTGGGGAAGGATAAGTTGAAGGAGGCTAAGGAGTTTGTGAAGCAGATGAAAACCAGGGGTTTTGTAGCTAACAAGAAAGCTGTGAAGGAAGTTCTGAAGGGTAAAAAAGTACCTATTGCTAAAATGGTCATCAACATTCTCTTTGCTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

469

Amino Acids

53.0

Weight (kDa)

6.33

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 334 - 383 2.5e-11 PPR repeat family
TPR_24 PF23276 341 - 421 1.6e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 350 - 449 2.7e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017793)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0459181
rosa_laevigata RLG00000025105
rosa_multiflora Rmu_sc0002435.1_g000015 Rmu_sc0004343.1_g000004 Rmu_sc0014208.1_g000004 Rmu_sc0024269.1_g000003
rosa_roxburghii Rroxscaffold_6G00420440
rosa_rugosa Rorug03G0034100 Rorug03G0034100
rosa_samantha Rh3CG095200 Rh3CG096000
rosa_wichuraiana Rw3G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 338
AciI CCGC 4 cut(s) 114, 338, 879, 1081
AclI AACGTT 1 cut(s) 690
AclWI GGATC 1 cut(s) 47
AcsI RAATTY 2 cut(s) 461, 746
AcuI CTGAAG 2 cut(s) 1023, 1373
AfaI GTAC 4 cut(s) 335, 374, 713, 1368
AfiI CCNNNNNNNGG 3 cut(s) 137, 946, 1260
AgeI ACCGGT 1 cut(s) 667
AgsI TTSAA 7 cut(s) 169, 269, 824, 854, 865, 916, 1276
AjnI CCWGG 3 cut(s) 130, 136, 1310
AleI CACNNNNGTG 1 cut(s) 539
AluBI AGCT 6 cut(s) 15, 362, 775, 1258, 1325, 1337
AluI AGCT 6 cut(s) 15, 362, 775, 1258, 1325, 1337
Alw26I GTCTC 2 cut(s) 394, 521
AlwI GGATC 1 cut(s) 47
AoxI GGCC 2 cut(s) 116, 963
ApaI GGGCCC 1 cut(s) 120
ApeKI GCWGC 2 cut(s) 443, 514
ApoI RAATTY 2 cut(s) 461, 746
AseI ATTAAT 1 cut(s) 642
AsiGI ACCGGT 1 cut(s) 667
Asp700I GAANNNNTTC 1 cut(s) 90
AspS9I GGNCC 8 cut(s) 116, 117, 134, 143, 454, 665, 963, 1053
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 2 cut(s) 187, 307
AvaII GGWCC 5 cut(s) 134, 143, 454, 665, 1053
BaeGI GKGCMC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 853
BbvI GCAGC 2 cut(s) 430, 526
BccI CCATC 5 cut(s) 493, 504, 625, 1021, 1189
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 3 cut(s) 132, 138, 1312
BcnI CCSGG 1 cut(s) 452
BcoDI GTCTC 2 cut(s) 394, 521
BfaI CTAG 2 cut(s) 36, 923
BfmI CTRYAG 4 cut(s) 444, 537, 660, 1120
BglII AGATCT 1 cut(s) 1032
BisI GCNGC 2 cut(s) 444, 515
BlsI GCNGC 2 cut(s) 445, 516
BmcAI AGTACT 1 cut(s) 713
Bme1390I CCNGG 4 cut(s) 132, 138, 452, 1312
Bme18I GGWCC 5 cut(s) 134, 143, 454, 665, 1053
BmgT120I GGNCC 8 cut(s) 116, 117, 134, 143, 454, 665, 963, 1053
BmiI GGNNCC 2 cut(s) 118, 456
BmrFI CCNGG 4 cut(s) 132, 138, 452, 1312
BmsI GCATC 5 cut(s) 207, 330, 331, 861, 1159
BpiI GAAGAC 1 cut(s) 853
BplI GAGNNNNNCTC 2 cut(s) 1022, 1054
BpmI CTGGAG 1 cut(s) 512
Bpu10I CCTNAGC 1 cut(s) 1284
BpuEI CTTGAG 4 cut(s) 488, 635, 791, 936
BpuMI CCSGG 1 cut(s) 452
BsaBI GATNNNNATC 1 cut(s) 1031
BsaI GGTCTC 2 cut(s) 394, 521
BsaJI CCNNGG 6 cut(s) 130, 354, 1041, 1056, 1093, 1311
BsaWI WCCGGW 2 cut(s) 145, 667
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 946, 1260
Bse118I RCCGGY 4 cut(s) 317, 344, 667, 1019
Bse1I ACTGG 3 cut(s) 444, 495, 770
Bse8I GATNNNNATC 1 cut(s) 1031
BseBI CCWGG 3 cut(s) 132, 138, 1312
BseDI CCNNGG 6 cut(s) 130, 354, 1041, 1056, 1093, 1311
BseGI GGATG 6 cut(s) 222, 346, 515, 736, 915, 1089
BseJI GATNNNNATC 1 cut(s) 1031
BseLI CCNNNNNNNGG 3 cut(s) 137, 946, 1260
BseMII CTCAG 4 cut(s) 735, 959, 1081, 1395
BseNI ACTGG 3 cut(s) 444, 495, 770
BseRI GAGGAG 3 cut(s) 344, 789, 794
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 2 cut(s) 430, 526
BseYI CCCAGC 2 cut(s) 1254, 1258
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 2 cut(s) 118, 965
BshTI ACCGGT 1 cut(s) 667
BsiSI CCGG 7 cut(s) 146, 158, 318, 345, 452, 668, 1020
BslFI GGGAC 3 cut(s) 113, 386, 670
BslI CCNNNNNNNGG 3 cut(s) 137, 946, 1260
BsmAI GTCTC 2 cut(s) 394, 521
BsmFI GGGAC 3 cut(s) 113, 386, 670
BsmI GAATGC 2 cut(s) 681, 1212
BsnI GGCC 2 cut(s) 118, 965
Bso31I GGTCTC 2 cut(s) 394, 521
Bsp120I GGGCCC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 3 cut(s) 39, 957, 1032
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 4 cut(s) 114, 338, 879, 1081
BspANI GGCC 2 cut(s) 118, 965
BspCNI CTCAG 4 cut(s) 734, 958, 1080, 1396
BspFNI CGCG 1 cut(s) 338
BspHI TCATGA 2 cut(s) 198, 259
BspLI GGNNCC 2 cut(s) 118, 456
BspMAI CTGCAG 2 cut(s) 448, 541
BspPI GGATC 1 cut(s) 47
BspTNI GGTCTC 2 cut(s) 394, 521
BsrFI RCCGGY 4 cut(s) 317, 344, 667, 1019
BsrI ACTGG 3 cut(s) 444, 495, 770
BssAI RCCGGY 4 cut(s) 317, 344, 667, 1019
BssECI CCNNGG 6 cut(s) 130, 354, 1041, 1056, 1093, 1311
BssMI GATC 3 cut(s) 39, 957, 1032
BssT1I CCWWGG 4 cut(s) 354, 1041, 1056, 1093
Bst2UI CCWGG 3 cut(s) 132, 138, 1312
Bst4CI ACNGT 3 cut(s) 75, 224, 901
Bst6I CTCTTC 2 cut(s) 197, 570
BstAPI GCANNNNNTGC 2 cut(s) 443, 1183
BstC8I GCNNGC 3 cut(s) 7, 116, 346
BstDEI CTNAG 5 cut(s) 721, 945, 1067, 1284, 1404
BstDSI CCRYGG 1 cut(s) 354
BstEII GGTNACC 1 cut(s) 313
BstENI CCTNNNNNAGG 2 cut(s) 135, 944
BstF5I GGATG 6 cut(s) 222, 346, 515, 736, 915, 1089
BstFNI CGCG 1 cut(s) 338
BstKTI GATC 3 cut(s) 42, 960, 1035
BstMAI GTCTC 2 cut(s) 394, 521
BstMBI GATC 3 cut(s) 39, 957, 1032
BstMWI GCNNNNNNNGC 3 cut(s) 443, 1109, 1183
BstNI CCWGG 3 cut(s) 132, 138, 1312
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 4 cut(s) 130, 136, 450, 1310
BstSFI CTRYAG 4 cut(s) 444, 537, 660, 1120
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 2 cut(s) 430, 526
BstV2I GAAGAC 1 cut(s) 853
BstX2I RGATCY 1 cut(s) 1032
BstYI RGATCY 1 cut(s) 1032
BsuRI GGCC 2 cut(s) 118, 965
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 6 cut(s) 222, 346, 515, 736, 915, 1089
BtsI GCAGTG 2 cut(s) 534, 546
BtsIMutI CAGTG 3 cut(s) 437, 534, 546
Cac8I GCNNGC 3 cut(s) 7, 116, 346
CciI TCATGA 2 cut(s) 198, 259
Cfr10I RCCGGY 4 cut(s) 317, 344, 667, 1019
Cfr13I GGNCC 8 cut(s) 116, 117, 134, 143, 454, 665, 963, 1053
Csp6I GTAC 4 cut(s) 334, 373, 712, 1367
CspAI ACCGGT 1 cut(s) 667
CviAII CATG 5 cut(s) 199, 260, 301, 355, 1024
CviQI GTAC 4 cut(s) 334, 373, 712, 1367
DdeI CTNAG 5 cut(s) 721, 945, 1067, 1284, 1404
DpnI GATC 3 cut(s) 41, 959, 1034
DpnII GATC 3 cut(s) 39, 957, 1032
Eam1104I CTCTTC 2 cut(s) 197, 570
EarI CTCTTC 2 cut(s) 197, 570
Eco130I CCWWGG 4 cut(s) 354, 1041, 1056, 1093
Eco24I GRGCYC 1 cut(s) 120
Eco31I GGTCTC 2 cut(s) 394, 521
Eco32I GATATC 1 cut(s) 1089
Eco47I GGWCC 5 cut(s) 134, 143, 454, 665, 1053
Eco57I CTGAAG 2 cut(s) 1023, 1373
Eco91I GGTNACC 1 cut(s) 313
EcoNI CCTNNNNNAGG 2 cut(s) 135, 944
EcoO65I GGTNACC 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 130, 136, 1310
EcoRV GATATC 1 cut(s) 1089
EcoT14I CCWWGG 4 cut(s) 354, 1041, 1056, 1093
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 4 cut(s) 354, 1041, 1056, 1093
FaeI CATG 5 cut(s) 202, 263, 304, 358, 1027
FaqI GGGAC 3 cut(s) 113, 386, 670
FatI CATG 5 cut(s) 198, 259, 300, 354, 1023
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 444, 515
FokI GGATG 6 cut(s) 229, 353, 522, 743, 922, 1096
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 2 cut(s) 444, 515
FspBI CTAG 2 cut(s) 36, 923
GluI GCNGC 2 cut(s) 444, 515
GsaI CCCAGC 2 cut(s) 1258, 1262
GsuI CTGGAG 1 cut(s) 512
HaeIII GGCC 2 cut(s) 118, 965
HapII CCGG 7 cut(s) 146, 158, 318, 345, 452, 668, 1020
Hin1II CATG 5 cut(s) 202, 263, 304, 358, 1027
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 7 cut(s) 195, 379, 704, 719, 811, 986, 1012
HpaII CCGG 7 cut(s) 146, 158, 318, 345, 452, 668, 1020
HphI GGTGA 2 cut(s) 187, 307
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 709, 718, 724, 1353
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 9 cut(s) 105, 185, 562, 878, 926, 1258, 1270, 1336, 1348
HpyCH4III ACNGT 3 cut(s) 75, 224, 901
HpyCH4IV ACGT 2 cut(s) 690, 928
HpyCH4V TGCA 8 cut(s) 220, 446, 476, 486, 539, 562, 874, 1210
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 1109, 1183
HpyF3I CTNAG 5 cut(s) 721, 945, 1067, 1284, 1404
HpySE526I ACGT 2 cut(s) 690, 928
Hsp92II CATG 5 cut(s) 202, 263, 304, 358, 1027
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
Kzo9I GATC 3 cut(s) 39, 957, 1032
LmnI GCTCC 2 cut(s) 20, 1108
Lsp1109I GCAGC 2 cut(s) 430, 526
LweI GCATC 5 cut(s) 207, 330, 331, 861, 1159
MaeI CTAG 2 cut(s) 36, 923
MaeII ACGT 2 cut(s) 690, 928
MaeIII GTNAC 3 cut(s) 313, 607, 793
MalI GATC 3 cut(s) 41, 959, 1034
MboI GATC 3 cut(s) 39, 957, 1032
MboII GAAGA 3 cut(s) 214, 587, 853
MflI RGATCY 1 cut(s) 1032
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 4 cut(s) 275, 394, 461, 746
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 741
MroNI GCCGGC 1 cut(s) 344
MroXI GAANNNNTTC 1 cut(s) 90
MseI TTAA 6 cut(s) 48, 233, 278, 546, 642, 675
MslI CAYNNNNRTG 2 cut(s) 539, 1022
MspA1I CMGCKG 2 cut(s) 1081, 1258
MspI CCGG 7 cut(s) 146, 158, 318, 345, 452, 668, 1020
MspR9I CCNGG 4 cut(s) 132, 138, 452, 1312
Mva1269I GAATGC 2 cut(s) 681, 1212
MvaI CCWGG 3 cut(s) 132, 138, 1312
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 443, 1109, 1183
NaeI GCCGGC 1 cut(s) 346
NciI CCSGG 1 cut(s) 452
NcoI CCATGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 418
NdeII GATC 3 cut(s) 39, 957, 1032
NgoMIV GCCGGC 1 cut(s) 344
NlaIII CATG 5 cut(s) 202, 263, 304, 358, 1027
NlaIV GGNNCC 2 cut(s) 118, 456
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 313
OliI CACNNNNGTG 1 cut(s) 539
PagI TCATGA 2 cut(s) 198, 259
PctI GAATGC 2 cut(s) 681, 1212
PdiI GCCGGC 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 90
PfeI GAWTC 6 cut(s) 195, 379, 704, 811, 986, 1012
PinAI ACCGGT 1 cut(s) 667
PkrI GCNGC 2 cut(s) 445, 516
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
PshBI ATTAAT 1 cut(s) 642
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 3 cut(s) 130, 136, 1310
PspEI GGTNACC 1 cut(s) 313
PspFI CCCAGC 2 cut(s) 1254, 1258
PspGI CCWGG 3 cut(s) 130, 136, 1310
PspN4I GGNNCC 2 cut(s) 118, 456
PspOMI GGGCCC 1 cut(s) 116
PspPI GGNCC 8 cut(s) 116, 117, 134, 143, 454, 665, 963, 1053
PstI CTGCAG 2 cut(s) 448, 541
PsuI RGATCY 1 cut(s) 1032
PvuII CAGCTG 1 cut(s) 1258
RsaI GTAC 4 cut(s) 335, 374, 713, 1368
RsaNI GTAC 4 cut(s) 334, 373, 712, 1367
RseI CAYNNNNRTG 2 cut(s) 539, 1022
SaqAI TTAA 6 cut(s) 48, 233, 278, 546, 642, 675
SatI GCNGC 2 cut(s) 444, 515
Sau3AI GATC 3 cut(s) 39, 957, 1032
Sau96I GGNCC 8 cut(s) 116, 117, 134, 143, 454, 665, 963, 1053
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 4 cut(s) 132, 138, 452, 1312
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 5 cut(s) 207, 330, 331, 861, 1159
SfcI CTRYAG 4 cut(s) 444, 537, 660, 1120
SinI GGWCC 5 cut(s) 134, 143, 454, 665, 1053
SmiMI CAYNNNNRTG 2 cut(s) 539, 1022
SmlI CTYRAG 4 cut(s) 467, 650, 806, 951
SmoI CTYRAG 4 cut(s) 467, 650, 806, 951
Sse9I AATT 4 cut(s) 275, 394, 461, 746
SsiI CCGC 4 cut(s) 114, 338, 879, 1081
SspMI CTAG 2 cut(s) 36, 923
StyD4I CCNGG 4 cut(s) 130, 136, 450, 1310
StyI CCWWGG 4 cut(s) 354, 1041, 1056, 1093
TaaI ACNGT 3 cut(s) 75, 224, 901
TaiI ACGT 2 cut(s) 693, 931
TaqI TCGA 1 cut(s) 573
TasI AATT 4 cut(s) 275, 394, 461, 746
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 6 cut(s) 195, 379, 704, 811, 986, 1012
Tru1I TTAA 6 cut(s) 48, 233, 278, 546, 642, 675
Tru9I TTAA 6 cut(s) 48, 233, 278, 546, 642, 675
TscAI CASTG 3 cut(s) 444, 541, 546
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 443, 514
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 87, 187, 194, 215, 1319
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 3 cut(s) 444, 541, 546
VpaK11BI GGWCC 5 cut(s) 134, 143, 454, 665, 1053
VspI ATTAAT 1 cut(s) 642
XagI CCTNNNNNAGG 2 cut(s) 135, 944
XapI RAATTY 2 cut(s) 461, 746
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 361
XmnI GAANNNNTTC 1 cut(s) 90
XspI CTAG 2 cut(s) 36, 923
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.