RLG00000025105

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017793
Physical Location & Seq
Forward (+)
42015430 .. 42017204
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025105

Sequence Viewer

Length: 1194 bp
ATGCATGGTAATCTGTTAAAAGACGGCTATGAGGACTTGGCTCATGAGATTGAAACTCAGTTAACGGAAAAGGACATTGTTCCCATGATTATTGTGGTCATCGACATCATTGAGGAGTACGCGGATGCTGGCAAGACCATGGAAGCTCTGGTGTTGTACCTGATTCTTTTGGTCTCTAATTGTGTCCCTAACCCTTACACATATAGTGTTCTCATCAGGGCATTGGCTGCAGACCCGGACCCCAAATTTCTTGAGGCTGCAAAAGAGTGCATACTGGAGATGATGGAAAAGGGATGGCAGCCCAATGCCAAGACCTACACTGCCTTGTTTAAGGCATTTGCTTTGCAGGAGAAGGTCGAAGAGGGTAAGAAGTTTCGGGAGCAAATGGAAGCTAAGGGCTTCGTGGCAGACAAGATGGCTGTGATTGATGCTCTCAAGGGGACTACAGGACCGGTGGTTAAAAGCATTCACAACATTCTCTTTGATGACTCAGAGTACTCCAACTCAGATGAGGATGATGATATAAAGAAATTCTACAAACTGATTGACTGGCAGAAGATTTCTCCGTATAGTTTTGTAACTCCTTTCCTCAAGATTCCTGATATTGAAAATGACCCAGAGAACCCCAAAGATTTTCAACAAGTCTTCAACAAGATGCACACTGCAGAAGGTTTTGTCAAAACTGTGGTAAGGATGTTCAAAATGCTAGAACGTGAAGGCTTCCTATCTCAGGCTCAAGAGATCAGGGCTAAGATATATTCTCTTTGTGAATCTAATGATATGCCTGAAGTCGTGATTCACACCGGCATGATGGAGATCTACTCCAAGGCTGACAGGTCCAAGGAGGCTCTCAGGGTGTTCCAGCGGATGATATCCCTTGGTGTTGCTCCCAACGCATACACCTATAGTGTTCTCATCATGGCACTCACTAAAGACCCCACCTTCCTTGATGATGCCAAGCAGTACGTTCTGGAAATGATGGGGAATGGAATGCAACCCAATGCCAAGACCTACACCACTTTGTTTTATGCTTTTGCCCAGCTGGGGAAGGATAAGTTGAAGGAGGCTAAGGAGTTTGTGAAGCAGATGAAAGCCAGGGGTTTTGTAGCTAACAAGAAAGCTGTGAAGGAAGTTCTGAAGGGTAAGAAAGCACCTATTGCTAAAATGGTCATTAACATTCTCTTTGCTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

398

Amino Acids

45.14

Weight (kDa)

5.66

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 36 - 76 4.7e-06 PPR repeat family
PPR_2 PF13041 262 - 310 2.3e-10 PPR repeat family
PPR_long PF17177 278 - 380 6.3e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017793)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0459181
rosa_laevigata RLG00000025105
rosa_multiflora Rmu_sc0002435.1_g000015 Rmu_sc0004343.1_g000004 Rmu_sc0014208.1_g000004 Rmu_sc0024269.1_g000003
rosa_roxburghii Rroxscaffold_6G00420440
rosa_rugosa Rorug03G0034100 Rorug03G0034100
rosa_samantha Rh3CG095200 Rh3CG096000
rosa_wichuraiana Rw3G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 122
AciI CCGC 2 cut(s) 122, 865
AcsI RAATTY 2 cut(s) 245, 530
AcuI CTGAAG 2 cut(s) 807, 1157
AfaI GTAC 4 cut(s) 119, 158, 497, 965
AfiI CCNNNNNNNGG 2 cut(s) 730, 1044
AgeI ACCGGT 1 cut(s) 451
AgsI TTSAA 6 cut(s) 53, 608, 638, 649, 700, 1060
AjnI CCWGG 1 cut(s) 1094
AluBI AGCT 5 cut(s) 146, 392, 1042, 1109, 1121
AluI AGCT 5 cut(s) 146, 392, 1042, 1109, 1121
Alw26I GTCTC 1 cut(s) 178
ApeKI GCWGC 3 cut(s) 227, 257, 298
ApoI RAATTY 2 cut(s) 245, 530
AsiGI ACCGGT 1 cut(s) 451
AspS9I GGNCC 3 cut(s) 238, 449, 837
AsuC2I CCSGG 1 cut(s) 236
AvaII GGWCC 3 cut(s) 238, 449, 837
BbsI GAAGAC 1 cut(s) 637
BbvI GCAGC 3 cut(s) 214, 244, 310
BccI CCATC 5 cut(s) 277, 288, 409, 805, 973
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 1096
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 1 cut(s) 707
BfmI CTRYAG 4 cut(s) 228, 444, 663, 904
BglII AGATCT 1 cut(s) 816
BisI GCNGC 3 cut(s) 228, 258, 299
BlsI GCNGC 3 cut(s) 229, 259, 300
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 2 cut(s) 236, 1096
Bme18I GGWCC 3 cut(s) 238, 449, 837
BmgT120I GGNCC 3 cut(s) 238, 449, 837
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 236, 1096
BmsI GCATC 4 cut(s) 115, 418, 645, 943
BpiI GAAGAC 1 cut(s) 637
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 2 cut(s) 393, 1068
BpuEI CTTGAG 4 cut(s) 272, 419, 575, 720
BpuMI CCSGG 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 815
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 5 cut(s) 138, 825, 840, 877, 1095
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 2 cut(s) 730, 1044
Bse118I RCCGGY 2 cut(s) 451, 803
Bse1I ACTGG 2 cut(s) 279, 554
Bse8I GATNNNNATC 1 cut(s) 815
BseBI CCWGG 1 cut(s) 1096
BseDI CCNNGG 5 cut(s) 138, 825, 840, 877, 1095
BseGI GGATG 5 cut(s) 130, 299, 520, 699, 873
BseJI GATNNNNATC 1 cut(s) 815
BseLI CCNNNNNNNGG 2 cut(s) 730, 1044
BseMII CTCAG 6 cut(s) 71, 504, 519, 743, 865, 1179
BseNI ACTGG 2 cut(s) 279, 554
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 214, 244, 310
BseYI CCCAGC 2 cut(s) 1038, 1042
Bsh1236I CGCG 1 cut(s) 122
BshTI ACCGGT 1 cut(s) 451
BsiSI CCGG 3 cut(s) 236, 452, 804
BslFI GGGAC 2 cut(s) 170, 454
BslI CCNNNNNNNGG 2 cut(s) 730, 1044
BsmAI GTCTC 1 cut(s) 178
BsmFI GGGAC 2 cut(s) 170, 454
BsmI GAATGC 2 cut(s) 465, 996
Bso31I GGTCTC 1 cut(s) 178
Bsp143I GATC 2 cut(s) 741, 816
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 2 cut(s) 122, 865
BspCNI CTCAG 6 cut(s) 70, 503, 518, 742, 864, 1180
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 2 cut(s) 232, 667
BspTNI GGTCTC 1 cut(s) 178
BsrFI RCCGGY 2 cut(s) 451, 803
BsrI ACTGG 2 cut(s) 279, 554
BssAI RCCGGY 2 cut(s) 451, 803
BssECI CCNNGG 5 cut(s) 138, 825, 840, 877, 1095
BssMI GATC 2 cut(s) 741, 816
BssT1I CCWWGG 4 cut(s) 138, 825, 840, 877
Bst2UI CCWGG 1 cut(s) 1096
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 2 cut(s) 227, 1157
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 9 cut(s) 57, 393, 490, 505, 729, 750, 851, 1068, 1188
BstDSI CCRYGG 1 cut(s) 138
BstENI CCTNNNNNAGG 1 cut(s) 728
BstF5I GGATG 5 cut(s) 130, 299, 520, 699, 873
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 2 cut(s) 744, 819
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 2 cut(s) 741, 816
BstMWI GCNNNNNNNGC 3 cut(s) 227, 893, 1157
BstNI CCWGG 1 cut(s) 1096
BstSCI CCNGG 2 cut(s) 234, 1094
BstSFI CTRYAG 4 cut(s) 228, 444, 663, 904
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 3 cut(s) 214, 244, 310
BstV2I GAAGAC 1 cut(s) 637
BstX2I RGATCY 1 cut(s) 816
BstYI RGATCY 1 cut(s) 816
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 5 cut(s) 130, 299, 520, 699, 873
BtsI GCAGTG 2 cut(s) 318, 660
BtsIMutI CAGTG 2 cut(s) 318, 660
Cac8I GCNNGC 1 cut(s) 130
CciI TCATGA 1 cut(s) 43
Cfr10I RCCGGY 2 cut(s) 451, 803
Cfr13I GGNCC 3 cut(s) 238, 449, 837
Csp6I GTAC 4 cut(s) 118, 157, 496, 964
CspAI ACCGGT 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviAII CATG 6 cut(s) 5, 44, 85, 139, 808, 919
CviQI GTAC 4 cut(s) 118, 157, 496, 964
DdeI CTNAG 9 cut(s) 57, 393, 490, 505, 729, 750, 851, 1068, 1188
DpnI GATC 2 cut(s) 743, 818
DpnII GATC 2 cut(s) 741, 816
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Eco130I CCWWGG 4 cut(s) 138, 825, 840, 877
Eco31I GGTCTC 1 cut(s) 178
Eco32I GATATC 1 cut(s) 873
Eco47I GGWCC 3 cut(s) 238, 449, 837
Eco57I CTGAAG 2 cut(s) 807, 1157
EcoNI CCTNNNNNAGG 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 1094
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 4 cut(s) 138, 825, 840, 877
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 138, 825, 840, 877
FaeI CATG 6 cut(s) 8, 47, 88, 142, 811, 922
FaqI GGGAC 2 cut(s) 170, 454
FatI CATG 6 cut(s) 4, 43, 84, 138, 807, 918
Fnu4HI GCNGC 3 cut(s) 228, 258, 299
FokI GGATG 5 cut(s) 137, 306, 527, 706, 880
Fsp4HI GCNGC 3 cut(s) 228, 258, 299
FspBI CTAG 1 cut(s) 707
GluI GCNGC 3 cut(s) 228, 258, 299
GsaI CCCAGC 2 cut(s) 1042, 1046
GsuI CTGGAG 1 cut(s) 296
HapII CCGG 3 cut(s) 236, 452, 804
Hin1II CATG 6 cut(s) 8, 47, 88, 142, 811, 922
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 5 cut(s) 163, 488, 595, 770, 796
HpaI GTTAAC 1 cut(s) 63
HpaII CCGG 3 cut(s) 236, 452, 804
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 3 cut(s) 493, 508, 1137
Hpy188III TCNNGA 8 cut(s) 44, 251, 377, 592, 599, 737, 793, 971
Hpy8I GTNNAC 1 cut(s) 63
HpyAV CCTTC 8 cut(s) 346, 662, 710, 952, 1042, 1054, 1120, 1132
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4IV ACGT 2 cut(s) 712, 966
HpyCH4V TGCA 8 cut(s) 4, 230, 260, 270, 346, 658, 665, 994
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 893, 1157
HpyF3I CTNAG 9 cut(s) 57, 393, 490, 505, 729, 750, 851, 1068, 1188
HpySE526I ACGT 2 cut(s) 712, 966
Hsp92II CATG 6 cut(s) 8, 47, 88, 142, 811, 922
KspAI GTTAAC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 741, 816
LmnI GCTCC 2 cut(s) 379, 892
Lsp1109I GCAGC 3 cut(s) 214, 244, 310
LweI GCATC 4 cut(s) 115, 418, 645, 943
MaeI CTAG 1 cut(s) 707
MaeII ACGT 2 cut(s) 712, 966
MaeIII GTNAC 1 cut(s) 577
MalI GATC 2 cut(s) 743, 818
MboI GATC 2 cut(s) 741, 816
MboII GAAGA 3 cut(s) 371, 568, 637
MflI RGATCY 1 cut(s) 816
MluCI AATT 3 cut(s) 178, 245, 530
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 8 cut(s) 25, 106, 247, 355, 505, 599, 838, 1057
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 5 cut(s) 17, 62, 330, 459, 1173
MslI CAYNNNNRTG 1 cut(s) 806
MspA1I CMGCKG 2 cut(s) 865, 1042
MspI CCGG 3 cut(s) 236, 452, 804
MspR9I CCNGG 2 cut(s) 236, 1096
Mva1269I GAATGC 2 cut(s) 465, 996
MvaI CCWGG 1 cut(s) 1096
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 3 cut(s) 227, 893, 1157
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 1 cut(s) 138
NdeII GATC 2 cut(s) 741, 816
NlaIII CATG 6 cut(s) 8, 47, 88, 142, 811, 922
NlaIV GGNNCC 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 43
PctI GAATGC 2 cut(s) 465, 996
PfeI GAWTC 4 cut(s) 163, 595, 770, 796
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 3 cut(s) 229, 259, 300
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
Psp6I CCWGG 1 cut(s) 1094
PspFI CCCAGC 2 cut(s) 1038, 1042
PspGI CCWGG 1 cut(s) 1094
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 3 cut(s) 238, 449, 837
PstI CTGCAG 2 cut(s) 232, 667
PsuI RGATCY 1 cut(s) 816
PvuII CAGCTG 1 cut(s) 1042
RsaI GTAC 4 cut(s) 119, 158, 497, 965
RsaNI GTAC 4 cut(s) 118, 157, 496, 964
RseI CAYNNNNRTG 1 cut(s) 806
SaqAI TTAA 5 cut(s) 17, 62, 330, 459, 1173
SatI GCNGC 3 cut(s) 228, 258, 299
Sau3AI GATC 2 cut(s) 741, 816
Sau96I GGNCC 3 cut(s) 238, 449, 837
ScaI AGTACT 1 cut(s) 497
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 236, 1096
SfaNI GCATC 4 cut(s) 115, 418, 645, 943
SfcI CTRYAG 4 cut(s) 228, 444, 663, 904
SinI GGWCC 3 cut(s) 238, 449, 837
SmiMI CAYNNNNRTG 1 cut(s) 806
SmlI CTYRAG 4 cut(s) 251, 434, 590, 735
SmoI CTYRAG 4 cut(s) 251, 434, 590, 735
Sse9I AATT 3 cut(s) 178, 245, 530
SsiI CCGC 2 cut(s) 122, 865
SspMI CTAG 1 cut(s) 707
StyD4I CCNGG 2 cut(s) 234, 1094
StyI CCWWGG 4 cut(s) 138, 825, 840, 877
TaaI ACNGT 1 cut(s) 685
TaiI ACGT 2 cut(s) 715, 969
TaqI TCGA 2 cut(s) 102, 357
TasI AATT 3 cut(s) 178, 245, 530
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 4 cut(s) 163, 595, 770, 796
Tru1I TTAA 5 cut(s) 17, 62, 330, 459, 1173
Tru9I TTAA 5 cut(s) 17, 62, 330, 459, 1173
TscAI CASTG 2 cut(s) 325, 667
TseI GCWGC 3 cut(s) 227, 257, 298
TspDTI ATGAA 1 cut(s) 1103
TspGWI ACGGA 2 cut(s) 80, 555
TspRI CASTG 2 cut(s) 325, 667
VpaK11BI GGWCC 3 cut(s) 238, 449, 837
XagI CCTNNNNNAGG 1 cut(s) 728
XapI RAATTY 2 cut(s) 245, 530
XcmI CCANNNNNNNNNTGG 2 cut(s) 91, 145
XspI CTAG 1 cut(s) 707
ZrmI AGTACT 1 cut(s) 497
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.