RchiOBHm_Chr3g0476451

Isocitrate dehydrogenase NADP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018047
Physical Location & Seq
Forward (+)
22408449 .. 22410076
1628 bp
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UTR
Exon/CDS
Intron
PRQ44187

Sequence Viewer

Length: 330 bp
ATGGTATTTGTCCCAGAAGGAGACACCCCTACGGAGTGTAATGTCCATGATTTCAAAGGTCCTGGTGTGGCCATCGCTATGTATAATGTTGATGAGTCTATTCGAGCTTTTGCTGACTCTTCCATGTCGATGGCATTTTCCAAGAAGTGGCCACTATACTTGAGCTCCAAAAACACAATTCTTAAGAAATATGATGGCAGGTTTAAGGACATATTCGAGGAGGTTTATGTGGAAAAGTGGAAGCAGAAGTTTGAAGAAAACTCTATTTGGTATGAGCATCGGCTAATAGATGACATGGTGGCTTATGCACTCAAAAGTGAGGGTGGGTAG

Protein Analysis

109

Amino Acids

12.65

Weight (kDa)

5.15

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 21 - 107 2.8e-13 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018047)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g25921
rosa_chinensis RchiOBHm_Chr1g0316931 RchiOBHm_Chr3g0476451
rosa_multiflora Rmu_sc0004138.1_g000004 Rmu_sc0007472.1_g000005
rosa_roxburghii Rroxscaffold_4G00330770
rosa_samantha Rh1CG023000 Rh3CG227600
rosa_wichuraiana Rw1G001690 Rw1G001700 Rw3G018430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 189
AccB7I CCANNNNNTGG 1 cut(s) 147
AcoI YGGCCR 2 cut(s) 69, 149
AfiI CCNNNNNNNGG 1 cut(s) 147
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 2 cut(s) 55, 254
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 2 cut(s) 107, 165
AluI AGCT 2 cut(s) 107, 165
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 1 cut(s) 15
AoxI GGCC 2 cut(s) 69, 149
AspS9I GGNCC 1 cut(s) 59
AvaII GGWCC 1 cut(s) 59
BalI TGGCCA 2 cut(s) 71, 151
BanII GRGCYC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 167
BccI CCATC 3 cut(s) 80, 124, 188
BciT130I CCWGG 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 15
BfrI CTTAAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 147
BseBI CCWGG 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 233
BshFI GGCC 2 cut(s) 71, 151
BsiHKAI GWGCWC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 15
BsnI GGCC 2 cut(s) 71, 151
Bsp1286I GDGCHC 1 cut(s) 167
BspANI GGCC 2 cut(s) 71, 151
BspMI ACCTGC 1 cut(s) 189
BspTI CTTAAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 124
BstAFI CTTAAG 1 cut(s) 182
BstMAI GTCTC 1 cut(s) 15
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 130
BsuRI GGCC 2 cut(s) 71, 151
BtgZI GCGATG 1 cut(s) 58
BveI ACCTGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 59
CspCI CAANNNNNGTGG 2 cut(s) 141, 176
CviAII CATG 3 cut(s) 47, 124, 295
CviJI RGCY 6 cut(s) 71, 107, 151, 165, 283, 302
CviKI_1 RGCY 6 cut(s) 71, 107, 151, 165, 283, 302
EaeI YGGCCR 2 cut(s) 69, 149
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 167
FaeI CATG 3 cut(s) 50, 127, 298
FatI CATG 3 cut(s) 46, 123, 294
FriOI GRGCYC 1 cut(s) 167
HaeIII GGCC 2 cut(s) 71, 151
Hin1II CATG 3 cut(s) 50, 127, 298
HinfI GANTC 2 cut(s) 95, 116
HpyAV CCTTC 1 cut(s) 11
HpyCH4V TGCA 1 cut(s) 308
Hsp92II CATG 3 cut(s) 50, 127, 298
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 4 cut(s) 27, 48, 75, 184
LweI GCATC 1 cut(s) 286
MboII GAAGA 2 cut(s) 111, 266
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 2 cut(s) 71, 151
MluCI AATT 1 cut(s) 177
MluNI TGGCCA 2 cut(s) 71, 151
MlyI GAGTC 2 cut(s) 104, 110
MnlI CCTC 3 cut(s) 211, 214, 313
Mox20I TGGCCA 2 cut(s) 71, 151
MscI TGGCCA 2 cut(s) 71, 151
MseI TTAA 2 cut(s) 183, 204
MslI CAYNNNNRTG 2 cut(s) 77, 128
Msp20I TGGCCA 2 cut(s) 71, 151
MspCI CTTAAG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 63
NlaIII CATG 3 cut(s) 50, 127, 298
PflMI CCANNNNNTGG 1 cut(s) 147
PleI GAGTC 2 cut(s) 103, 110
PpsI GAGTC 2 cut(s) 103, 110
PpuMI RGGWCCY 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspPI GGNCC 1 cut(s) 59
PspPPI RGGWCCY 1 cut(s) 59
RseI CAYNNNNRTG 2 cut(s) 77, 128
SacI GAGCTC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 183, 204
Sau96I GGNCC 1 cut(s) 59
SchI GAGTC 2 cut(s) 104, 110
ScrFI CCNGG 1 cut(s) 63
SduI GDGCHC 1 cut(s) 167
SetI ASST 5 cut(s) 61, 109, 167, 203, 225
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 77, 128
SmlI CTYRAG 2 cut(s) 160, 182
SmoI CTYRAG 2 cut(s) 160, 182
Sse9I AATT 1 cut(s) 177
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 61
TaqI TCGA 3 cut(s) 103, 128, 216
TasI AATT 1 cut(s) 177
Tru1I TTAA 2 cut(s) 183, 204
Tru9I TTAA 2 cut(s) 183, 204
TspGWI ACGGA 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 147
Vha464I CTTAAG 1 cut(s) 182
VpaK11BI GGWCC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.