RchiOBHm_Chr4g0384841

Protein disulfide isomerase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014853
Physical Location & Seq
Forward (+)
219445 .. 219876
432 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35839

Sequence Viewer

Length: 432 bp
ATGGCGAGTCGATTCGTTCTCGTGTTCACACTCAGCGCCCTGCTCCTCCTCTCCTCCCGCCCAGCTCTCTCCAAGGACGCCGTCGTCGATGACGACGACGAGGATCTCAGCTTCCTCAAAGAGCCGACCGAGCAACAGGGCCATAGCCATGACGCGGCGCTGCACTATCCAGATTCGGACGAATTTGACGGCGAGAGCTACGACGACGCCGACGACTTCTCTGATTTCGACGAGGGTGATCAGGAGGCGTACAAGGAACCCGAGGACTTCGTCATTTTGAAGGGGGCGAACTTCAGCAAGACGATTAAGAAGAACAGGTTCGTGCTGGTTGAGTTATACGCGCCGTGGTGCGGACACTGCCAAGCCTTTGGCGATGTGCACAATCAAGTCGATCACAGGGGAGATGTAACCCCACTCGTTGTCGTACCATGA

Protein Analysis

143

Amino Acids

16.02

Weight (kDa)

4.27

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 91 - 128 3.3e-09 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AccII CGCG 2 cut(s) 155, 341
AciI CCGC 3 cut(s) 58, 155, 351
AclWI GGATC 1 cut(s) 111
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 277
AcyI GRCGYC 2 cut(s) 78, 207
AfaI GTAC 2 cut(s) 251, 426
AfiI CCNNNNNNNGG 2 cut(s) 154, 350
AgsI TTSAA 1 cut(s) 280
AluBI AGCT 3 cut(s) 65, 111, 198
AluI AGCT 3 cut(s) 65, 111, 198
Alw21I GWGCWC 1 cut(s) 381
Alw44I GTGCAC 1 cut(s) 377
AlwI GGATC 1 cut(s) 111
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 139
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 317
AspLEI GCGC 3 cut(s) 38, 160, 343
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 381
BauI CACGAG 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 147
BceAI ACGGC 3 cut(s) 65, 205, 328
BclI TGATCA 1 cut(s) 238
BfoI RGCGCY 2 cut(s) 39, 161
BisI GCNGC 2 cut(s) 156, 161
BlsI GCNGC 2 cut(s) 157, 162
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 258
BsaHI GRCGYC 2 cut(s) 78, 207
BsaJI CCNNGG 3 cut(s) 72, 261, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 350
BseDI CCNNGG 3 cut(s) 72, 261, 344
BseLI CCNNNNNNNGG 2 cut(s) 154, 350
BseMII CTCAG 2 cut(s) 46, 121
BseRI GAGGAG 3 cut(s) 35, 38, 43
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 2 cut(s) 155, 341
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 141
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 154, 350
BsnI GGCC 1 cut(s) 141
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 3 cut(s) 103, 238, 391
BspACI CCGC 3 cut(s) 58, 155, 351
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 2 cut(s) 45, 120
BspFNI CGCG 2 cut(s) 155, 341
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 1 cut(s) 111
BssECI CCNNGG 3 cut(s) 72, 261, 344
BssMI GATC 3 cut(s) 103, 238, 391
BssNI GRCGYC 2 cut(s) 78, 207
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 20
BstACI GRCGYC 2 cut(s) 78, 207
BstDEI CTNAG 2 cut(s) 32, 107
BstDSI CCRYGG 1 cut(s) 344
BstFNI CGCG 2 cut(s) 155, 341
BstH2I RGCGCY 2 cut(s) 39, 161
BstHHI GCGC 3 cut(s) 38, 160, 343
BstKTI GATC 3 cut(s) 106, 241, 394
BstMBI GATC 3 cut(s) 103, 238, 391
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 130, 357
BstSLI GKGCMC 1 cut(s) 381
BstUI CGCG 2 cut(s) 155, 341
BstV1I GCAGC 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 344
BtgZI GCGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 1 cut(s) 355
CfoI GCGC 3 cut(s) 38, 160, 343
Cfr13I GGNCC 1 cut(s) 139
CseI GACGC 3 cut(s) 86, 161, 215
Csp6I GTAC 2 cut(s) 250, 425
CviAII CATG 2 cut(s) 149, 429
CviJI RGCY 7 cut(s) 65, 111, 124, 141, 147, 198, 365
CviKI_1 RGCY 7 cut(s) 65, 111, 124, 141, 147, 198, 365
CviQI GTAC 2 cut(s) 250, 425
DdeI CTNAG 2 cut(s) 32, 107
DpnI GATC 3 cut(s) 105, 240, 393
DpnII GATC 3 cut(s) 103, 238, 391
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 2 cut(s) 152, 432
FaiI YATR 4 cut(s) 144, 150, 337, 430
FatI CATG 2 cut(s) 148, 428
FauI CCCGC 1 cut(s) 65
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 2 cut(s) 156, 161
Fsp4HI GCNGC 2 cut(s) 156, 161
GlaI GCGC 3 cut(s) 37, 159, 342
GluI GCNGC 2 cut(s) 156, 161
GsaI CCCAGC 1 cut(s) 65
HaeII RGCGCY 2 cut(s) 39, 161
HaeIII GGCC 1 cut(s) 141
HgaI GACGC 3 cut(s) 86, 161, 215
HhaI GCGC 3 cut(s) 38, 160, 343
Hin1I GRCGYC 2 cut(s) 78, 207
Hin1II CATG 2 cut(s) 152, 432
Hin6I GCGC 3 cut(s) 36, 158, 341
HinP1I GCGC 3 cut(s) 36, 158, 341
HinfI GANTC 3 cut(s) 7, 12, 173
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 2 cut(s) 27, 379
Hpy188I TCNGA 2 cut(s) 178, 223
Hpy188III TCNNGA 2 cut(s) 170, 242
Hpy8I GTNNAC 2 cut(s) 27, 379
Hpy99I CGWCG 8 cut(s) 86, 89, 98, 101, 206, 209, 215, 233
HpyAV CCTTC 1 cut(s) 274
HpyCH4V TGCA 2 cut(s) 163, 379
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 357
HpyF3I CTNAG 2 cut(s) 32, 107
Hsp92I GRCGYC 2 cut(s) 78, 207
Hsp92II CATG 2 cut(s) 152, 432
HspAI GCGC 3 cut(s) 36, 158, 341
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 3 cut(s) 103, 238, 391
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 53, 75, 122, 183, 227, 301, 311, 382
Lsp1109I GCAGC 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 406
MalI GATC 3 cut(s) 105, 240, 393
MboI GATC 3 cut(s) 103, 238, 391
MboII GAAGA 1 cut(s) 322
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 1 cut(s) 182
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 8 cut(s) 56, 59, 64, 94, 125, 226, 238, 256
MroXI GAANNNNTTC 1 cut(s) 317
MseI TTAA 1 cut(s) 306
MslI CAYNNNNRTG 1 cut(s) 147
MvnI CGCG 2 cut(s) 155, 341
MwoI GCNNNNNNNGC 2 cut(s) 130, 357
NdeII GATC 3 cut(s) 103, 238, 391
NlaIII CATG 2 cut(s) 152, 432
NlaIV GGNNCC 1 cut(s) 258
PcsI WCGNNNNNNNCGW 5 cut(s) 84, 90, 93, 207, 210
PdmI GAANNNNTTC 1 cut(s) 317
PfeI GAWTC 2 cut(s) 12, 173
PflFI GACNNNGTC 2 cut(s) 80, 269
PkrI GCNGC 2 cut(s) 157, 162
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 61
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 139
PsuI RGATCY 1 cut(s) 103
PsyI GACNNNGTC 2 cut(s) 80, 269
RsaI GTAC 2 cut(s) 251, 426
RsaNI GTAC 2 cut(s) 250, 425
RseI CAYNNNNRTG 1 cut(s) 147
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 2 cut(s) 156, 161
Sau3AI GATC 3 cut(s) 103, 238, 391
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 1 cut(s) 16
SduI GDGCHC 1 cut(s) 381
SetI ASST 4 cut(s) 67, 113, 200, 320
SmiMI CAYNNNNRTG 1 cut(s) 147
Sse9I AATT 1 cut(s) 182
SsiI CCGC 3 cut(s) 58, 155, 351
StyI CCWWGG 1 cut(s) 72
TaqI TCGA 4 cut(s) 10, 87, 228, 390
TaqII GACCGA 1 cut(s) 143
TasI AATT 1 cut(s) 182
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 2 cut(s) 12, 173
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 362
TseI GCWGC 1 cut(s) 160
TspRI CASTG 1 cut(s) 362
Tth111I GACNNNGTC 2 cut(s) 80, 269
VneI GTGCAC 1 cut(s) 377
XapI RAATTY 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.