RchiOBHm_Chr4g0395591

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011598
Physical Location & Seq
Forward (+)
11380974 .. 11382077
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36803

Sequence Viewer

Length: 1104 bp
ATGTCACTTAAGGACCTTTGCAGGCGTGAAGCAGACTGGAACTTATATAAGGTCGCTTGCGAGGAAGATGCATATTTGAAATCCTTTGACACCGACGTCCAAAGCCGAACCACAAAGATCATCAGCACCCTTCAGAATGGAGGTGTTTCAACTGATTATGTGAGTGATGCCATAGCATATGTGACAGAATTTAACAAGCAGACTGTGGACGCAATCAGGGTGTGCAGAGAAGATATAACGAGTGGTGCGTGGAAGAATAAGAAGAAGAAGATGAAGAGTGAAGAAAGCTTTAAGGTGGTGGTGGAGGAGTACGTCGACAACAGCCAGGAGATGCTGGATTTCTGCTCTGTATTGGACAAGTTCCTGAAATCGGCTCGCAACAACCATTTTGAGATTGAACAAGTAATTCAACAGTGCGAGATGGAGACTGATCCCGAGGGAGGAAATAAGTACGTGAGGATTTCGAGGAAATTGAAGAGTCTCAAGGCTAGTGCACACCATTACGATTCTGCAAACAGATTACTAGAAAAGATTCAAAGCTTGCAGGCCAAGAATCAAGGGCTGATCGATAAACTGAAAATTGAATATGAGAAGCTGGCAAACAAGCGGAAGAGCGTTCGCGCTTGGAAGAAGGCGGTGAATATGATATTCATCACCACAGTGGCTGCCCAAGTTATTTCTGCAGTCGCGGTGCTAGCTATGGCTAATCCAGCTGCTTCTGCAGCTATTGCTGTTACCATTGCCCCAGTAATTGCTGGACATCATTGGGCTCTTGGTTTGCTGGCAGAATACGAAAAAAGTTTGAATGATGACAAAGTGATACTGCAAAAAATGCATGATGCAAGTGTGTTTTGCATCAAGGAGTTGGGCGACGTAAAGTTTCTCATCGATAAGGTTGTAAATGAGATAGATGCTGTCTTGTTTCACGATTGTCATGCTGATTCTGCCATGGAGGAGGGACAAGTGGAAAACGCTATCAAGAATATTAAGAGTACGCTGGACATGTTTCTCAAGAAAATTGAGATATTGGAGGAAAAAGGAGAGACTTGCAGCTCTGAGGTATTGAAATCAAGGGATAAGGTTGTGAGGAGCATGAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

367

Amino Acids

41.34

Weight (kDa)

6.73

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 26 - 362 6.6e-41 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 621, 689
AciI CCGC 3 cut(s) 607, 635, 689
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 3 cut(s) 311, 452, 994
AfiI CCNNNNNNNGG 2 cut(s) 370, 440
AflII CTTAAG 1 cut(s) 8
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 9 cut(s) 79, 150, 398, 410, 475, 536, 584, 805, 1066
AjnI CCWGG 1 cut(s) 324
AleI CACNNNNGTG 1 cut(s) 659
AluBI AGCT 7 cut(s) 288, 540, 595, 698, 713, 725, 1053
AluI AGCT 7 cut(s) 288, 540, 595, 698, 713, 725, 1053
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 3 cut(s) 419, 485, 1037
Alw44I GTGCAC 1 cut(s) 492
AlwI GGATC 1 cut(s) 425
AlwNI CAGNNNCTG 1 cut(s) 665
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 1 cut(s) 546
ApaLI GTGCAC 1 cut(s) 492
ApeKI GCWGC 4 cut(s) 665, 713, 722, 1050
ApoI RAATTY 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 2 cut(s) 646, 649
AsuNHI GCTAGC 1 cut(s) 694
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 772
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 4 cut(s) 652, 700, 734, 1062
BccI CCATC 1 cut(s) 415
BcgI CGANNNNNNTGC 4 cut(s) 50, 84, 917, 951
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 3 cut(s) 419, 485, 1037
BfaI CTAG 3 cut(s) 489, 524, 695
BfmI CTRYAG 2 cut(s) 681, 720
BfrI CTTAAG 1 cut(s) 8
BisI GCNGC 4 cut(s) 666, 714, 723, 1051
BlsI GCNGC 4 cut(s) 667, 715, 724, 1052
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 13
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 740
BmsI GCATC 6 cut(s) 58, 157, 321, 829, 864, 901
BmtI GCTAGC 1 cut(s) 698
BmuI ACTGGG 1 cut(s) 740
BpuEI CTTGAG 2 cut(s) 467, 995
Bsa29I ATCGAT 2 cut(s) 567, 888
BsaAI YACGTR 1 cut(s) 454
BsaBI GATNNNNATC 1 cut(s) 650
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 435, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 440
Bse1I ACTGG 2 cut(s) 41, 746
Bse3DI GCAATG 1 cut(s) 738
Bse8I GATNNNNATC 1 cut(s) 650
BseBI CCWGG 1 cut(s) 326
BseCI ATCGAT 2 cut(s) 567, 888
BseDI CCNNGG 2 cut(s) 435, 948
BseJI GATNNNNATC 1 cut(s) 650
BseLI CCNNNNNNNGG 2 cut(s) 370, 440
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 1 cut(s) 1047
BseNI ACTGG 2 cut(s) 41, 746
BseRI GAGGAG 3 cut(s) 320, 968, 1102
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 4 cut(s) 652, 700, 734, 1062
BsgI GTGCAG 1 cut(s) 244
Bsh1236I CGCG 2 cut(s) 621, 689
BshFI GGCC 1 cut(s) 548
BshVI ATCGAT 2 cut(s) 567, 888
BsiHKAI GWGCWC 1 cut(s) 496
BsiHKCI CYCGRG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 972
BslI CCNNNNNNNGG 2 cut(s) 370, 440
BsmAI GTCTC 3 cut(s) 419, 485, 1037
BsmFI GGGAC 1 cut(s) 972
BsnI GGCC 1 cut(s) 548
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 496, 772
Bsp143I GATC 3 cut(s) 117, 430, 564
Bsp19I CCATGG 1 cut(s) 948
BspACI CCGC 3 cut(s) 607, 635, 689
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 1048
BspDI ATCGAT 2 cut(s) 567, 888
BspFNI CGCG 2 cut(s) 621, 689
BspMAI CTGCAG 2 cut(s) 685, 724
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 1 cut(s) 425
BspQI GCTCTTC 1 cut(s) 605
BspTI CTTAAG 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 738
BsrI ACTGG 2 cut(s) 41, 746
BssECI CCNNGG 2 cut(s) 435, 948
BssMI GATC 3 cut(s) 117, 430, 564
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 205, 414, 661
Bst6I CTCTTC 3 cut(s) 269, 470, 605
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 8
BstAPI GCANNNNNTGC 2 cut(s) 728, 832
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 8 cut(s) 23, 58, 376, 542, 546, 597, 696, 783
BstDEI CTNAG 1 cut(s) 1056
BstDSI CCRYGG 1 cut(s) 948
BstFNI CGCG 2 cut(s) 621, 689
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 3 cut(s) 120, 433, 567
BstMAI GTCTC 3 cut(s) 419, 485, 1037
BstMBI GATC 3 cut(s) 117, 430, 564
BstMWI GCNNNNNNNGC 7 cut(s) 695, 710, 719, 722, 728, 832, 944
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 1006
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 2 cut(s) 681, 720
BstSLI GKGCMC 1 cut(s) 496
BstUI CGCG 2 cut(s) 621, 689
BstV1I GCAGC 4 cut(s) 652, 700, 734, 1062
Bsu15I ATCGAT 2 cut(s) 567, 888
BsuRI GGCC 1 cut(s) 548
BsuTUI ATCGAT 2 cut(s) 567, 888
BtgI CCRYGG 1 cut(s) 948
BtsIMutI CAGTG 2 cut(s) 419, 666
Cac8I GCNNGC 8 cut(s) 23, 58, 376, 542, 546, 597, 696, 783
CaiI CAGNNNCTG 1 cut(s) 665
CfoI GCGC 1 cut(s) 623
Cfr13I GGNCC 1 cut(s) 13
ClaI ATCGAT 2 cut(s) 567, 888
CseI GACGC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 310, 451, 993
CviAII CATG 5 cut(s) 836, 935, 949, 1003, 1093
CviQI GTAC 3 cut(s) 310, 451, 993
DdeI CTNAG 1 cut(s) 1056
DpnI GATC 3 cut(s) 119, 432, 566
DpnII GATC 3 cut(s) 117, 430, 564
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 269, 470, 605
EarI CTCTTC 3 cut(s) 269, 470, 605
Eco130I CCWWGG 1 cut(s) 948
Eco24I GRGCYC 1 cut(s) 772
Eco47I GGWCC 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 948
EcoT22I ATGCAT 2 cut(s) 73, 837
EcoT38I GRGCYC 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 948
FaeI CATG 5 cut(s) 839, 938, 952, 1006, 1096
FaqI GGGAC 1 cut(s) 972
FatI CATG 5 cut(s) 835, 934, 948, 1002, 1092
FauNDI CATATG 1 cut(s) 178
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 4 cut(s) 666, 714, 723, 1051
FriOI GRGCYC 1 cut(s) 772
Fsp4HI GCNGC 4 cut(s) 666, 714, 723, 1051
FspBI CTAG 3 cut(s) 489, 524, 695
GlaI GCGC 1 cut(s) 622
GluI GCNGC 4 cut(s) 666, 714, 723, 1051
HaeIII GGCC 1 cut(s) 548
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 623
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 5 cut(s) 839, 938, 952, 1006, 1096
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 2 cut(s) 286, 538
HinfI GANTC 5 cut(s) 478, 506, 532, 553, 941
HphI GGTGA 2 cut(s) 646, 649
Hpy166II GTNNAC 3 cut(s) 208, 316, 494
Hpy188I TCNGA 2 cut(s) 135, 1057
Hpy188III TCNNGA 5 cut(s) 364, 434, 926, 979, 1012
Hpy8I GTNNAC 3 cut(s) 208, 316, 494
Hpy99I CGWCG 3 cut(s) 98, 317, 875
HpyAV CCTTC 2 cut(s) 140, 625
HpyCH4III ACNGT 3 cut(s) 205, 414, 661
HpyCH4IV ACGT 4 cut(s) 96, 312, 453, 873
HpyF10VI GCNNNNNNNGC 7 cut(s) 695, 710, 719, 722, 728, 832, 944
HpyF3I CTNAG 1 cut(s) 1056
HpySE526I ACGT 4 cut(s) 96, 312, 453, 873
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 5 cut(s) 839, 938, 952, 1006, 1096
HspAI GCGC 1 cut(s) 621
Kzo9I GATC 3 cut(s) 117, 430, 564
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 1 cut(s) 1089
Lsp1109I GCAGC 4 cut(s) 652, 700, 734, 1062
LweI GCATC 6 cut(s) 58, 157, 321, 829, 864, 901
MaeI CTAG 3 cut(s) 489, 524, 695
MaeII ACGT 4 cut(s) 96, 312, 453, 873
MaeIII GTNAC 3 cut(s) 3, 181, 733
MalI GATC 3 cut(s) 119, 432, 566
MboI GATC 3 cut(s) 117, 430, 564
MhlI GDGCHC 2 cut(s) 496, 772
MluCI AATT 6 cut(s) 188, 405, 470, 579, 750, 1017
MlyI GAGTC 1 cut(s) 487
Mph1103I ATGCAT 2 cut(s) 73, 837
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 4 cut(s) 9, 192, 291, 987
MslI CAYNNNNRTG 1 cut(s) 659
MspA1I CMGCKG 1 cut(s) 713
MspCI CTTAAG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 2 cut(s) 621, 689
MwoI GCNNNNNNNGC 7 cut(s) 695, 710, 719, 722, 728, 832, 944
NcoI CCATGG 1 cut(s) 948
NdeI CATATG 1 cut(s) 178
NdeII GATC 3 cut(s) 117, 430, 564
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 5 cut(s) 839, 938, 952, 1006, 1096
NmuCI GTSAC 2 cut(s) 3, 181
NsiI ATGCAT 2 cut(s) 73, 837
NspI RCATGY 1 cut(s) 1006
OliI CACNNNNGTG 1 cut(s) 659
PciI ACATGT 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 4 cut(s) 506, 532, 553, 941
PkrI GCNGC 4 cut(s) 667, 715, 724, 1052
PleI GAGTC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 486
Ppu21I YACGTR 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 13
PscI ACATGT 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspPI GGNCC 1 cut(s) 13
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 2 cut(s) 685, 724
PstNI CAGNNNCTG 1 cut(s) 665
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 3 cut(s) 311, 452, 994
RsaNI GTAC 3 cut(s) 310, 451, 993
RseI CAYNNNNRTG 1 cut(s) 659
SalI GTCGAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 4 cut(s) 9, 192, 291, 987
SatI GCNGC 4 cut(s) 666, 714, 723, 1051
Sau3AI GATC 3 cut(s) 117, 430, 564
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 496, 772
SfaNI GCATC 6 cut(s) 58, 157, 321, 829, 864, 901
SfcI CTRYAG 2 cut(s) 681, 720
SinI GGWCC 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 3 cut(s) 8, 482, 1010
SmoI CTYRAG 3 cut(s) 8, 482, 1010
Sse9I AATT 6 cut(s) 188, 405, 470, 579, 750, 1017
SsiI CCGC 3 cut(s) 607, 635, 689
SspI AATATT 1 cut(s) 985
SspMI CTAG 3 cut(s) 489, 524, 695
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 948
TaaI ACNGT 3 cut(s) 205, 414, 661
TaiI ACGT 4 cut(s) 99, 315, 456, 876
TaqI TCGA 4 cut(s) 315, 464, 567, 888
TasI AATT 6 cut(s) 188, 405, 470, 579, 750, 1017
TfiI GAWTC 4 cut(s) 506, 532, 553, 941
Tru1I TTAA 4 cut(s) 9, 192, 291, 987
Tru9I TTAA 4 cut(s) 9, 192, 291, 987
TscAI CASTG 2 cut(s) 419, 666
TseFI GTSAC 2 cut(s) 3, 181
TseI GCWGC 4 cut(s) 665, 713, 722, 1050
Tsp45I GTSAC 2 cut(s) 3, 181
TspDTI ATGAA 2 cut(s) 287, 640
TspRI CASTG 2 cut(s) 419, 666
Vha464I CTTAAG 1 cut(s) 8
VneI GTGCAC 1 cut(s) 492
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 3 cut(s) 489, 524, 695
ZraI GACGTC 1 cut(s) 97
Zsp2I ATGCAT 2 cut(s) 73, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.