Rh4AG222200

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011598
Physical Location & Seq
Forward (+)
52136656 .. 52138595
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG222200.1

Sequence Viewer

Length: 1140 bp
ATGGGTGGAAAGAGAGAGTTAATCCGCAGGTCGTGGATGTCACTTAAGGAACTTTGCAGCCGTGAAGCAGACTGGAACTCATATAAGGTCGCTTGCGAGGAATATGCAGATTTGAAATCCTTTGACGCCGACGTCCAAAGCCGAACCACAAAAGTCATCAGCACCCTTCAGAATGGAGGTGTTTCAACTGATTTTGTGAGTGAAGCCATAGCATATGTGACAGAATTTAACAAGCACACTGTGGAAGCAATCAGGGTGTACAGAGAAGATATAACGAGTGGTGTGTCGAAGAATAAGAAGAAGAAGATGAAGAGCGAAGAAAGCTTTAGGATGGTGGTGGAGGAGTACGTCCACAACAGCGAGGAGATGCTGGATTTCTGCTCTGTATTGGACAAGTTCCTGAAATCGGCTCGCAAAAACCATTTTGAGATTGAACAAGTAATTCAACAGTGCGAGATGGAGACTGATCCCGAGGGAGGAAATAAGTACGTGAGGATTTCGAAGAAATTGAAGAGTCTCAAGGCTATTGCACACCATTCCGATTCTGCAGACAGATTACTAGAAAAGATTCAAAGCTTACAGGCCAAGAATAAAGGGTTGATCGATAACCTGAACAATGAATATAAGAAGCTGGCAAACAAGCAGAAGAGCGGTCGCGCATGGAGGAAGGTGGTGAATATTATATTCATCACCATAGTGGCTACTCAGTTGATTTCTGCAGTCGCGATGCTAGCTATGGCTAATCCAGCTGCAGCAGCTGTTGCTGGTACCATTCCCCCAGTAATTGCTGGATATCAATGTGTTCTCGATTTGCTGACAGAATACGAAAAAATTTTGGATAATGACAACGTGATACTGCAAACAATGCATGGTGCAAGTGTGTTTTTCATCAGTGAGTTGGGCGACGTAAAGTTTCTCATCCATAAGGTTGTAAATGAGATGGATGCTGTCTTGTTTCATGATTGTCACGCTGATTCTGCCATGGCGGAGGGACAAGTGGAAAACGCTATCAAGAATATTAAGAGTAAGCTGGACATGTTTCTCAAGAAAATTGAGGATTTGGAGGAAAAAGGAGAGACTTGCAGCTCTGAGGTATTGAAATCAAGGGATGAAGTGAGGAGCATGATGAATAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

379

Amino Acids

42.76

Weight (kDa)

6.54

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 38 - 371 4.8e-38 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AatII GACGTC 1 cut(s) 135
Acc36I ACCTGC 1 cut(s) 18
Acc65I GGTACC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 767
AccBSI CCGCTC 1 cut(s) 651
AccII CGCG 2 cut(s) 657, 725
AciI CCGC 3 cut(s) 25, 651, 986
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 2 cut(s) 224, 831
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 2 cut(s) 126, 132
AdeI CACNNNGTG 1 cut(s) 241
AfaI GTAC 4 cut(s) 260, 347, 488, 769
AfiI CCNNNNNNNGG 2 cut(s) 406, 476
AflII CTTAAG 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 7 cut(s) 115, 186, 434, 446, 511, 572, 1099
AleI CACNNNNGTG 1 cut(s) 695
AluBI AGCT 8 cut(s) 324, 576, 631, 734, 749, 758, 1030, 1086
AluI AGCT 8 cut(s) 324, 576, 631, 734, 749, 758, 1030, 1086
Alw26I GTCTC 3 cut(s) 455, 521, 1070
AlwI GGATC 1 cut(s) 461
AlwNI CAGNNNCTG 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 5 cut(s) 57, 749, 752, 755, 1083
ApoI RAATTY 2 cut(s) 224, 831
Asp700I GAANNNNTTC 1 cut(s) 567
Asp718I GGTACC 1 cut(s) 767
AspLEI GCGC 1 cut(s) 659
AsuHPI GGTGA 2 cut(s) 682, 685
AsuII TTCGAA 1 cut(s) 500
AsuNHI GCTAGC 1 cut(s) 730
AvaI CYCGRG 1 cut(s) 470
BanI GGYRCC 1 cut(s) 767
BbvI GCAGC 5 cut(s) 69, 736, 764, 767, 1095
BccI CCATC 3 cut(s) 325, 451, 934
BceAI ACGGC 1 cut(s) 45
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BcoDI GTCTC 3 cut(s) 455, 521, 1070
BfaI CTAG 2 cut(s) 560, 731
BfmI CTRYAG 3 cut(s) 546, 717, 750
BfrI CTTAAG 1 cut(s) 44
BfuAI ACCTGC 1 cut(s) 18
BisI GCNGC 5 cut(s) 58, 750, 753, 756, 1084
BlsI GCNGC 5 cut(s) 59, 751, 754, 757, 1085
BmeT110I CYCGRG 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 769
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 3 cut(s) 357, 717, 934
BmtI GCTAGC 1 cut(s) 734
BmuI ACTGGG 1 cut(s) 773
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 503, 1028
Bsa29I ATCGAT 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 490
BsaHI GRCGYC 2 cut(s) 126, 132
BsaJI CCNNGG 2 cut(s) 471, 981
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 476
Bse1I ACTGG 2 cut(s) 77, 779
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 2 cut(s) 471, 981
BseGI GGATG 5 cut(s) 42, 336, 918, 949, 1114
BseLI CCNNNNNNNGG 2 cut(s) 406, 476
BseMII CTCAG 2 cut(s) 719, 1080
BseNI ACTGG 2 cut(s) 77, 779
BseRI GAGGAG 3 cut(s) 356, 377, 1132
BseXI GCAGC 5 cut(s) 69, 736, 764, 767, 1095
Bsh1236I CGCG 2 cut(s) 657, 725
Bsh1285I CGRYCG 1 cut(s) 655
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 1 cut(s) 767
BshVI ATCGAT 1 cut(s) 603
BsiEI CGRYCG 1 cut(s) 655
BsiHKCI CYCGRG 1 cut(s) 470
BslFI GGGAC 1 cut(s) 1005
BslI CCNNNNNNNGG 2 cut(s) 406, 476
BsmAI GTCTC 3 cut(s) 455, 521, 1070
BsmFI GGGAC 1 cut(s) 1005
BsnI GGCC 1 cut(s) 584
BsoBI CYCGRG 1 cut(s) 470
Bsp119I TTCGAA 1 cut(s) 500
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 2 cut(s) 466, 600
Bsp19I CCATGG 1 cut(s) 981
Bsp68I TCGCGA 1 cut(s) 725
BspACI CCGC 3 cut(s) 25, 651, 986
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 2 cut(s) 718, 1081
BspDI ATCGAT 1 cut(s) 603
BspFNI CGCG 2 cut(s) 657, 725
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 1 cut(s) 769
BspMAI CTGCAG 3 cut(s) 550, 721, 754
BspMI ACCTGC 1 cut(s) 18
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 2 cut(s) 305, 641
BspT104I TTCGAA 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 767
BspTI CTTAAG 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 651
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 77, 779
BssECI CCNNGG 2 cut(s) 471, 981
BssMI GATC 2 cut(s) 466, 600
BssNI GRCGYC 2 cut(s) 126, 132
BssT1I CCWWGG 1 cut(s) 981
Bst4CI ACNGT 2 cut(s) 241, 450
Bst6I CTCTTC 3 cut(s) 305, 506, 641
BstACI GRCGYC 2 cut(s) 126, 132
BstAFI CTTAAG 1 cut(s) 44
BstAPI GCANNNNNTGC 2 cut(s) 761, 865
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 4 cut(s) 94, 412, 633, 732
BstDEI CTNAG 2 cut(s) 705, 1089
BstDSI CCRYGG 1 cut(s) 981
BstF5I GGATG 5 cut(s) 42, 336, 918, 949, 1114
BstFNI CGCG 2 cut(s) 657, 725
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 2 cut(s) 469, 603
BstMAI GTCTC 3 cut(s) 455, 521, 1070
BstMBI GATC 2 cut(s) 466, 600
BstMCI CGRYCG 1 cut(s) 655
BstMWI GCNNNNNNNGC 7 cut(s) 321, 731, 746, 755, 761, 865, 977
BstNSI RCATGY 1 cut(s) 1039
BstSFI CTRYAG 3 cut(s) 546, 717, 750
BstUI CGCG 2 cut(s) 657, 725
BstV1I GCAGC 5 cut(s) 69, 736, 764, 767, 1095
Bsu15I ATCGAT 1 cut(s) 603
BsuRI GGCC 1 cut(s) 584
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 981
BtgZI GCGATG 1 cut(s) 740
BtsCI GGATG 5 cut(s) 42, 336, 918, 949, 1114
BtsIMutI CAGTG 3 cut(s) 237, 455, 898
BtuMI TCGCGA 1 cut(s) 725
BveI ACCTGC 1 cut(s) 18
Cac8I GCNNGC 4 cut(s) 94, 412, 633, 732
CaiI CAGNNNCTG 1 cut(s) 758
CciI TCATGA 1 cut(s) 958
CfoI GCGC 1 cut(s) 659
ClaI ATCGAT 1 cut(s) 603
CseI GACGC 1 cut(s) 134
Csp6I GTAC 4 cut(s) 259, 346, 487, 768
CviAII CATG 6 cut(s) 660, 869, 959, 982, 1036, 1123
CviQI GTAC 4 cut(s) 259, 346, 487, 768
DdeI CTNAG 2 cut(s) 705, 1089
DpnI GATC 2 cut(s) 468, 602
DpnII GATC 2 cut(s) 466, 600
DraIII CACNNNGTG 1 cut(s) 241
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 305, 506, 641
EarI CTCTTC 3 cut(s) 305, 506, 641
EciI GGCGGA 1 cut(s) 1001
Eco130I CCWWGG 1 cut(s) 981
Eco32I GATATC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 470
EcoRV GATATC 1 cut(s) 794
EcoT14I CCWWGG 1 cut(s) 981
EcoT22I ATGCAT 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 981
FaeI CATG 6 cut(s) 663, 872, 962, 985, 1039, 1126
FaqI GGGAC 1 cut(s) 1005
FatI CATG 6 cut(s) 659, 868, 958, 981, 1035, 1122
FauNDI CATATG 1 cut(s) 214
Fnu4HI GCNGC 5 cut(s) 58, 750, 753, 756, 1084
FokI GGATG 5 cut(s) 49, 343, 905, 956, 1121
Fsp4HI GCNGC 5 cut(s) 58, 750, 753, 756, 1084
FspBI CTAG 2 cut(s) 560, 731
GlaI GCGC 1 cut(s) 658
GluI GCNGC 5 cut(s) 58, 750, 753, 756, 1084
HaeIII GGCC 1 cut(s) 584
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 2 cut(s) 126, 132
Hin1II CATG 6 cut(s) 663, 872, 962, 985, 1039, 1126
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HindIII AAGCTT 2 cut(s) 322, 574
HinfI GANTC 4 cut(s) 514, 542, 568, 974
HphI GGTGA 2 cut(s) 682, 685
Hpy166II GTNNAC 2 cut(s) 259, 352
Hpy188I TCNGA 3 cut(s) 171, 541, 1090
Hpy188III TCNNGA 7 cut(s) 400, 470, 724, 806, 959, 1012, 1045
Hpy8I GTNNAC 2 cut(s) 259, 352
Hpy99I CGWCG 2 cut(s) 134, 908
HpyAV CCTTC 2 cut(s) 176, 661
HpyCH4III ACNGT 2 cut(s) 241, 450
HpyCH4IV ACGT 5 cut(s) 132, 348, 489, 849, 906
HpyF10VI GCNNNNNNNGC 7 cut(s) 321, 731, 746, 755, 761, 865, 977
HpyF3I CTNAG 2 cut(s) 705, 1089
HpySE526I ACGT 5 cut(s) 132, 348, 489, 849, 906
Hsp92I GRCGYC 2 cut(s) 126, 132
Hsp92II CATG 6 cut(s) 663, 872, 962, 985, 1039, 1126
HspAI GCGC 1 cut(s) 657
KpnI GGTACC 1 cut(s) 771
Kzo9I GATC 2 cut(s) 466, 600
LguI GCTCTTC 2 cut(s) 305, 641
LmnI GCTCC 1 cut(s) 1119
Lsp1109I GCAGC 5 cut(s) 69, 736, 764, 767, 1095
LweI GCATC 3 cut(s) 357, 717, 934
MaeI CTAG 2 cut(s) 560, 731
MaeII ACGT 5 cut(s) 132, 348, 489, 849, 906
MaeIII GTNAC 3 cut(s) 39, 217, 965
MalI GATC 2 cut(s) 468, 602
MbiI CCGCTC 1 cut(s) 651
MboI GATC 2 cut(s) 466, 600
MluCI AATT 6 cut(s) 224, 441, 506, 783, 831, 1050
MlyI GAGTC 1 cut(s) 523
Mph1103I ATGCAT 1 cut(s) 870
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 4 cut(s) 20, 45, 228, 1020
MslI CAYNNNNRTG 1 cut(s) 695
MspA1I CMGCKG 2 cut(s) 749, 758
MspCI CTTAAG 1 cut(s) 44
MvnI CGCG 2 cut(s) 657, 725
MwoI GCNNNNNNNGC 7 cut(s) 321, 731, 746, 755, 761, 865, 977
NcoI CCATGG 1 cut(s) 981
NdeI CATATG 1 cut(s) 214
NdeII GATC 2 cut(s) 466, 600
NheI GCTAGC 1 cut(s) 730
NlaIII CATG 6 cut(s) 663, 872, 962, 985, 1039, 1126
NlaIV GGNNCC 1 cut(s) 769
NmuCI GTSAC 3 cut(s) 39, 217, 965
NruI TCGCGA 1 cut(s) 725
NsiI ATGCAT 1 cut(s) 870
NspI RCATGY 1 cut(s) 1039
NspV TTCGAA 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 695
PagI TCATGA 1 cut(s) 958
PciI ACATGT 1 cut(s) 1035
PciSI GCTCTTC 2 cut(s) 305, 641
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 3 cut(s) 542, 568, 974
PkrI GCNGC 5 cut(s) 59, 751, 754, 757, 1085
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
Ppu21I YACGTR 1 cut(s) 490
PscI ACATGT 1 cut(s) 1035
PspN4I GGNNCC 1 cut(s) 769
PstI CTGCAG 3 cut(s) 550, 721, 754
PstNI CAGNNNCTG 1 cut(s) 758
PvuII CAGCTG 2 cut(s) 749, 758
RruI TCGCGA 1 cut(s) 725
RsaI GTAC 4 cut(s) 260, 347, 488, 769
RsaNI GTAC 4 cut(s) 259, 346, 487, 768
RseI CAYNNNNRTG 1 cut(s) 695
SapI GCTCTTC 2 cut(s) 305, 641
SaqAI TTAA 4 cut(s) 20, 45, 228, 1020
SatI GCNGC 5 cut(s) 58, 750, 753, 756, 1084
Sau3AI GATC 2 cut(s) 466, 600
SchI GAGTC 1 cut(s) 523
SfaNI GCATC 3 cut(s) 357, 717, 934
SfcI CTRYAG 3 cut(s) 546, 717, 750
SfuI TTCGAA 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 3 cut(s) 44, 518, 1043
SmoI CTYRAG 3 cut(s) 44, 518, 1043
Sse9I AATT 6 cut(s) 224, 441, 506, 783, 831, 1050
SsiI CCGC 3 cut(s) 25, 651, 986
SspI AATATT 2 cut(s) 679, 1018
SspMI CTAG 2 cut(s) 560, 731
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 2 cut(s) 241, 450
TaiI ACGT 5 cut(s) 135, 351, 492, 852, 909
TaqI TCGA 4 cut(s) 287, 500, 603, 807
TasI AATT 6 cut(s) 224, 441, 506, 783, 831, 1050
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 3 cut(s) 542, 568, 974
Tru1I TTAA 4 cut(s) 20, 45, 228, 1020
Tru9I TTAA 4 cut(s) 20, 45, 228, 1020
TscAI CASTG 3 cut(s) 244, 455, 898
TseFI GTSAC 3 cut(s) 39, 217, 965
TseI GCWGC 5 cut(s) 57, 749, 752, 755, 1083
Tsp45I GTSAC 3 cut(s) 39, 217, 965
TspDTI ATGAA 6 cut(s) 323, 633, 676, 877, 947, 1125
TspRI CASTG 3 cut(s) 244, 455, 898
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 2 cut(s) 224, 831
XceI RCATGY 1 cut(s) 1039
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 560, 731
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.