Rw4G019140

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011598
Physical Location & Seq
Forward (+)
42277564 .. 42280956
3393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019140.1

Sequence Viewer

Length: 735 bp
ATGGGTGGAAAGAGAGAGTTAATCCGCAGGTCGTGGATGTCACTTAAGGACCTTTGCAGGCGTGAAGCAGACTGGAACTCATATAAGGTCGCTTGCGAGGAAGATGCAGATTTGAAATCCTTTGACGCCGACGTCCAAAGCCGAACCACAAAGGTCATCAGCACCCTTCAGAATGGAGGTGTTTCAACTGATTATGTGAGTGATGCCATAGCATATGTGACAGAATTTAACAAGCAGACTGTGGACGCAATCAGGGTATGCAGAGAAGATATAACGAGTGGTGCGTGGAAGACTAAGAAGAAGAAGATGAAGAGTGAAGAAAGCTTTAAGGTGGTGGTGGAGGAGTACGTCGACAACAGCCAGGAGATGCTGGATTTCTGCTCTGTATTGGACAAGTTCCTGAAATCGGCTCGCAACAACCATTTTGAGATTGAACAAGTAATTCAACAGTGCGAGATGGAGACTGATCCCGAGGGAGGAAATAAGTACGTGAGGATTTCGAGGAAATTGAAGAGTCTCAAGGCTAGTGCACACCATTACGATTCTGCAAACAGATTACTAGAAAAGATTCAAAGCTTGCAGGCCAAGAATCAAGGGCTGATCGATAAACTGAAAATTGAATATGAGAAGCTGGCAAACAAGCGGAGGAGCGTTCGCGCTTGGAAGAAGGCGGTCTATCAAGCAATTGTTGCTTATACAAATAACAACAACTTGGTGTTTTTCTCGCTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

244

Amino Acids

28.21

Weight (kDa)

9.01

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 38 - 227 2.8e-18 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AatII GACGTC 1 cut(s) 135
Acc36I ACCTGC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 351
AccII CGCG 1 cut(s) 657
AciI CCGC 3 cut(s) 25, 643, 671
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 2 cut(s) 126, 132
AfaI GTAC 2 cut(s) 347, 488
AfiI CCNNNNNNNGG 2 cut(s) 406, 476
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 7 cut(s) 115, 186, 434, 446, 511, 572, 620
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 3 cut(s) 324, 576, 631
AluI AGCT 3 cut(s) 324, 576, 631
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 2 cut(s) 455, 521
Alw44I GTGCAC 1 cut(s) 528
AlwI GGATC 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 1 cut(s) 582
ApaLI GTGCAC 1 cut(s) 528
ApoI RAATTY 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 532
BccI CCATC 1 cut(s) 451
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 455, 521
BfaI CTAG 2 cut(s) 525, 560
BfrI CTTAAG 1 cut(s) 44
BfuAI ACCTGC 1 cut(s) 18
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 3 cut(s) 94, 193, 357
BpiI GAAGAC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 490
BsaHI GRCGYC 2 cut(s) 126, 132
BsaJI CCNNGG 1 cut(s) 471
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 476
Bse1I ACTGG 1 cut(s) 77
BseBI CCWGG 1 cut(s) 362
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 406, 476
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 356, 661
BseSI GKGCMC 1 cut(s) 532
Bsh1236I CGCG 1 cut(s) 657
BshFI GGCC 1 cut(s) 584
BshVI ATCGAT 1 cut(s) 603
BsiHKAI GWGCWC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 470
BslI CCNNNNNNNGG 2 cut(s) 406, 476
BsmAI GTCTC 2 cut(s) 455, 521
BsnI GGCC 1 cut(s) 584
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 532
Bsp143I GATC 2 cut(s) 466, 600
BspACI CCGC 3 cut(s) 25, 643, 671
BspANI GGCC 1 cut(s) 584
BspDI ATCGAT 1 cut(s) 603
BspFNI CGCG 1 cut(s) 657
BspMI ACCTGC 1 cut(s) 18
BspPI GGATC 1 cut(s) 461
BspTI CTTAAG 1 cut(s) 44
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 2 cut(s) 466, 600
BssNI GRCGYC 2 cut(s) 126, 132
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 241, 450
Bst6I CTCTTC 2 cut(s) 305, 506
BstACI GRCGYC 2 cut(s) 126, 132
BstAFI CTTAAG 1 cut(s) 44
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 490
BstC8I GCNNGC 6 cut(s) 59, 94, 412, 578, 582, 633
BstDEI CTNAG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 42
BstFNI CGCG 1 cut(s) 657
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 2 cut(s) 469, 603
BstMAI GTCTC 2 cut(s) 455, 521
BstMBI GATC 2 cut(s) 466, 600
BstMWI GCNNNNNNNGC 1 cut(s) 689
BstNI CCWGG 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 1 cut(s) 657
BstV2I GAAGAC 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 603
BsuRI GGCC 1 cut(s) 584
BsuTUI ATCGAT 1 cut(s) 603
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 455
BveI ACCTGC 1 cut(s) 18
Cac8I GCNNGC 6 cut(s) 59, 94, 412, 578, 582, 633
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 49
ClaI ATCGAT 1 cut(s) 603
CseI GACGC 2 cut(s) 134, 254
Csp6I GTAC 2 cut(s) 346, 487
CviJI RGCY 9 cut(s) 141, 324, 360, 410, 524, 576, 584, 598, 631
CviKI_1 RGCY 9 cut(s) 141, 324, 360, 410, 524, 576, 584, 598, 631
CviQI GTAC 2 cut(s) 346, 487
DdeI CTNAG 1 cut(s) 294
DpnI GATC 2 cut(s) 468, 602
DpnII GATC 2 cut(s) 466, 600
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
Eam1104I CTCTTC 2 cut(s) 305, 506
EarI CTCTTC 2 cut(s) 305, 506
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 360
FauNDI CATATG 1 cut(s) 214
FblI GTMKAC 1 cut(s) 351
FokI GGATG 1 cut(s) 49
FspBI CTAG 2 cut(s) 525, 560
GlaI GCGC 1 cut(s) 658
HaeIII GGCC 1 cut(s) 584
HgaI GACGC 2 cut(s) 134, 254
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 2 cut(s) 126, 132
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 2 cut(s) 322, 574
HinfI GANTC 4 cut(s) 514, 542, 568, 589
Hpy166II GTNNAC 3 cut(s) 244, 352, 530
Hpy188I TCNGA 1 cut(s) 171
Hpy188III TCNNGA 2 cut(s) 400, 470
Hpy8I GTNNAC 3 cut(s) 244, 352, 530
Hpy99I CGWCG 2 cut(s) 134, 353
HpyAV CCTTC 2 cut(s) 176, 661
HpyCH4III ACNGT 2 cut(s) 241, 450
HpyCH4IV ACGT 3 cut(s) 132, 348, 489
HpyCH4V TGCA 6 cut(s) 57, 107, 261, 530, 548, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 689
HpyF3I CTNAG 1 cut(s) 294
HpySE526I ACGT 3 cut(s) 132, 348, 489
Hsp92I GRCGYC 2 cut(s) 126, 132
HspAI GCGC 1 cut(s) 657
Kzo9I GATC 2 cut(s) 466, 600
LmnI GCTCC 1 cut(s) 648
LweI GCATC 3 cut(s) 94, 193, 357
MaeI CTAG 2 cut(s) 525, 560
MaeII ACGT 3 cut(s) 132, 348, 489
MaeIII GTNAC 2 cut(s) 39, 217
MalI GATC 2 cut(s) 468, 602
MboI GATC 2 cut(s) 466, 600
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 5 cut(s) 224, 441, 506, 615, 684
MlyI GAGTC 1 cut(s) 523
MnlI CCTC 8 cut(s) 91, 170, 334, 466, 470, 486, 495, 639
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 4 cut(s) 20, 45, 228, 327
MspCI CTTAAG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 684
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 657
MwoI GCNNNNNNNGC 1 cut(s) 689
NdeI CATATG 1 cut(s) 214
NdeII GATC 2 cut(s) 466, 600
NmuCI GTSAC 2 cut(s) 39, 217
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 3 cut(s) 542, 568, 589
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
Ppu21I YACGTR 1 cut(s) 490
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspPI GGNCC 1 cut(s) 49
PspPPI RGGWCCY 1 cut(s) 49
RsaI GTAC 2 cut(s) 347, 488
RsaNI GTAC 2 cut(s) 346, 487
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 4 cut(s) 20, 45, 228, 327
Sau3AI GATC 2 cut(s) 466, 600
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 532
SfaNI GCATC 3 cut(s) 94, 193, 357
SinI GGWCC 1 cut(s) 49
SmlI CTYRAG 2 cut(s) 44, 518
SmoI CTYRAG 2 cut(s) 44, 518
Sse9I AATT 5 cut(s) 224, 441, 506, 615, 684
SsiI CCGC 3 cut(s) 25, 643, 671
SspMI CTAG 2 cut(s) 525, 560
StyD4I CCNGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 241, 450
TaiI ACGT 3 cut(s) 135, 351, 492
TaqI TCGA 3 cut(s) 351, 500, 603
TasI AATT 5 cut(s) 224, 441, 506, 615, 684
TfiI GAWTC 3 cut(s) 542, 568, 589
Tru1I TTAA 4 cut(s) 20, 45, 228, 327
Tru9I TTAA 4 cut(s) 20, 45, 228, 327
TscAI CASTG 1 cut(s) 455
TseFI GTSAC 2 cut(s) 39, 217
Tsp45I GTSAC 2 cut(s) 39, 217
TspDTI ATGAA 1 cut(s) 323
TspRI CASTG 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 44
VneI GTGCAC 1 cut(s) 528
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 224
XmiI GTMKAC 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 525, 560
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.