Rroxscaffold_5G00362670

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011598
Physical Location & Seq
Forward (+)
43686893 .. 43688401
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00362670.1

Sequence Viewer

Length: 462 bp
ATGATATTCATCACCACAGTGGCAGCCCAAGTTATTTCTGCAGTCGCGGTGCTAGCTATGGCTAATCCAGTTGCTTCTGGAGCTATTGCTGCTTCCATTGCCCCAGTAATTGGTGGACATCAGTGGGCTCTTGGTTTGCTGGCACAATACGAAAAAAGTTTGAATGATGACGACGTGATACTGCAAAAAATGCATGATGCAAGTGTGTTTTGCATCAAGGAGTTGGGCGACGTAAAGTTTCTCATCGATAAGGTTGTAAATGAGATCGATGCTGTCTTGTTTCACGATTGTCATGCTGATTCTGCCATGGAGGAGGGACAAGTGGAAAACGCTATCAAGAATATTAAGAGTAAGCTGGACATGTTTCTCAAGAAAATTGAGCAATTGGAGGAAAAAGGAGAGACTTGCAGCTCTGAGGTATTGAAATCAAGGGATAAGGTTGTGAGGAGCATGAAAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

153

Amino Acids

16.61

Weight (kDa)

5.17

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 1 - 148 1.7e-16 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 110
AccII CGCG 1 cut(s) 47
AciI CCGC 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 110
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 2 cut(s) 163, 424
AjiI CACGTC 1 cut(s) 175
AleI CACNNNNGTG 1 cut(s) 17
AluBI AGCT 4 cut(s) 56, 83, 355, 411
AluI AGCT 4 cut(s) 56, 83, 355, 411
Alw26I GTCTC 1 cut(s) 395
ApeKI GCWGC 3 cut(s) 23, 89, 408
AsuHPI GGTGA 1 cut(s) 4
AsuNHI GCTAGC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 130
BbvI GCAGC 3 cut(s) 35, 76, 420
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 3 cut(s) 24, 90, 409
BlsI GCNGC 3 cut(s) 25, 91, 410
BmgBI CACGTC 1 cut(s) 175
BmrI ACTGGG 1 cut(s) 98
BmsI GCATC 3 cut(s) 187, 222, 259
BmtI GCTAGC 1 cut(s) 56
BmuI ACTGGG 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 353
Bsa29I ATCGAT 2 cut(s) 246, 267
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 68, 104
Bse3DI GCAATG 1 cut(s) 96
Bse8I GATNNNNATC 1 cut(s) 8
BseCI ATCGAT 2 cut(s) 246, 267
BseDI CCNNGG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 2 cut(s) 68, 104
BseRI GAGGAG 2 cut(s) 326, 460
BseXI GCAGC 3 cut(s) 35, 76, 420
Bsh1236I CGCG 1 cut(s) 47
BshVI ATCGAT 2 cut(s) 246, 267
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 1 cut(s) 264
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 406
BspDI ATCGAT 2 cut(s) 246, 267
BspFNI CGCG 1 cut(s) 47
BspMAI CTGCAG 1 cut(s) 43
BspOI GCTAGC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 2 cut(s) 68, 104
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 190
BstC8I GCNNGC 2 cut(s) 54, 141
BstDEI CTNAG 1 cut(s) 414
BstDSI CCRYGG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 47
BstKTI GATC 1 cut(s) 267
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 264
BstMWI GCNNNNNNNGC 6 cut(s) 53, 80, 89, 98, 190, 302
BstNSI RCATGY 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 3 cut(s) 35, 76, 420
Bsu15I ATCGAT 2 cut(s) 246, 267
BsuTUI ATCGAT 2 cut(s) 246, 267
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 24, 128
Cac8I GCNNGC 2 cut(s) 54, 141
ClaI ATCGAT 2 cut(s) 246, 267
CviAII CATG 5 cut(s) 194, 293, 307, 361, 451
CviJI RGCY 7 cut(s) 26, 56, 62, 83, 128, 355, 411
CviKI_1 RGCY 7 cut(s) 26, 56, 62, 83, 128, 355, 411
DdeI CTNAG 1 cut(s) 414
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 5 cut(s) 197, 296, 310, 364, 454
FaiI YATR 7 cut(s) 59, 195, 294, 308, 362, 452, 460
FaqI GGGAC 1 cut(s) 330
FatI CATG 5 cut(s) 193, 292, 306, 360, 450
Fnu4HI GCNGC 3 cut(s) 24, 90, 409
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 24, 90, 409
FspBI CTAG 1 cut(s) 53
GluI GCNGC 3 cut(s) 24, 90, 409
GsuI CTGGAG 1 cut(s) 99
Hin1II CATG 5 cut(s) 197, 296, 310, 364, 454
HinfI GANTC 1 cut(s) 299
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 1 cut(s) 415
Hpy188III TCNNGA 4 cut(s) 78, 284, 337, 370
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 2 cut(s) 176, 233
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 2 cut(s) 174, 231
HpyCH4V TGCA 6 cut(s) 41, 184, 193, 200, 213, 408
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 80, 89, 98, 190, 302
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 2 cut(s) 174, 231
Hsp92II CATG 5 cut(s) 197, 296, 310, 364, 454
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 2 cut(s) 80, 447
LpnPI CCDG 5 cut(s) 63, 81, 117, 125, 341
Lsp1109I GCAGC 3 cut(s) 35, 76, 420
LweI GCATC 3 cut(s) 187, 222, 259
MaeI CTAG 1 cut(s) 53
MaeII ACGT 2 cut(s) 174, 231
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MfeI CAATTG 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 3 cut(s) 108, 375, 383
MnlI CCTC 5 cut(s) 304, 307, 382, 409, 438
Mph1103I ATGCAT 1 cut(s) 195
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 17
MunI CAATTG 1 cut(s) 383
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 6 cut(s) 53, 80, 89, 98, 190, 302
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 264
NheI GCTAGC 1 cut(s) 52
NlaIII CATG 5 cut(s) 197, 296, 310, 364, 454
NsiI ATGCAT 1 cut(s) 195
NspI RCATGY 1 cut(s) 364
OliI CACNNNNGTG 1 cut(s) 17
PciI ACATGT 1 cut(s) 360
PfeI GAWTC 1 cut(s) 299
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 3 cut(s) 25, 91, 410
PscI ACATGT 1 cut(s) 360
PstI CTGCAG 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 3 cut(s) 24, 90, 409
Sau3AI GATC 1 cut(s) 264
SduI GDGCHC 1 cut(s) 130
SetI ASST 9 cut(s) 58, 85, 177, 234, 255, 357, 413, 420, 441
SfaNI GCATC 3 cut(s) 187, 222, 259
SfcI CTRYAG 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 3 cut(s) 108, 375, 383
SsiI CCGC 1 cut(s) 47
SspI AATATT 1 cut(s) 343
SspMI CTAG 1 cut(s) 53
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 2 cut(s) 177, 234
TaqI TCGA 2 cut(s) 246, 267
TasI AATT 3 cut(s) 108, 375, 383
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 24, 128
TseI GCWGC 3 cut(s) 23, 89, 408
TspRI CASTG 2 cut(s) 24, 128
Van91I CCANNNNNTGG 1 cut(s) 110
XceI RCATGY 1 cut(s) 364
XspI CTAG 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.