RchiOBHm_Chr4g0398691

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015079
Physical Location & Seq
Forward (+)
15287153 .. 15289183
2031 bp
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UTR
Exon/CDS
Intron
PRQ37085

Sequence Viewer

Length: 633 bp
ATGACAAAACGTTTACCCCTCTCCTCTGTTTCCCACAAAACTAGAAGTCTAGAACCGAAAAGAAAAAGGCAAAAACAGAACATGGACACCGTCGTCTCCAATTCAGCACTACTATCCAAACCACCCTTCCCTCCAGTCCGTTCAGTTACTTGCAACTCTTATTCACCAACCACTTCTCTCAATCTCTCGTCAAAGAAGCACTTCTCCAGCTTGTTCTACACCCAAAAGAAACAAGTTGGTCATCGCAACTCTTCTTGTACAGCTCCCAAGTTTTCAATTGCTTCAAGTGCTGCTATTCAGGAAATCAACGAGACCCAGTTTCGGGATACGGTTCTTAACTCGGACCGTCCGGTTCTTGTCGAGTTTGTTGCCAACTGGTGCGGTCCTTGCCGCTTAATTTCTCCCGCTATGGAATGGGTTGCTCAGGAATATAAAGACAGATTAGCAGTTGTTAAGATCGATCATGATGCAAACCCACGATTAATTGAAGAATACAAAGTTTATGGTTTGCCTGCTTTGATTCTCTTCAAGAATGGACAGGAAGTTCCAGAAAGTAGAAGAGAGGGTGCAATTACAAAAGTTAAGCTTAAAGAGTATGTGGATGCTTTTTTGGAATCTATATCAGTCGCATAG

Protein Analysis

210

Amino Acids

23.66

Weight (kDa)

9.59

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 100 - 201 2.5e-27 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50320
fragaria_vesca FvH4_4g06760
malus_domestica MD15G1433100.v1.1
prunus_persica Prupe.1G070700_v2.0.a1
pyrus_communis pycom13g21100 pycom16g20440
rosa_chinensis RchiOBHm_Chr4g0398691
rosa_laevigata RLG00000009331
rosa_multiflora Rmu_sc0008600.1_g000003
rosa_roxburghii Rroxscaffold_5G00343650
rosa_rugosa Rorug04G0010900
rosa_samantha Rh4AG088600 Rh4BG085600 Rh4CG096700 Rh4DG080200
rosa_wichuraiana Rw4G007330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AciI CCGC 3 cut(s) 381, 391, 405
AclI AACGTT 1 cut(s) 10
AfaI GTAC 1 cut(s) 259
AfiI CCNNNNNNNGG 2 cut(s) 321, 322
AgsI TTSAA 4 cut(s) 276, 285, 488, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AloI GAACNNNNNNTCC 2 cut(s) 528, 560
AluBI AGCT 3 cut(s) 210, 263, 586
AluI AGCT 3 cut(s) 210, 263, 586
Alw26I GTCTC 2 cut(s) 100, 305
ApeKI GCWGC 1 cut(s) 290
AseI ATTAAT 1 cut(s) 482
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 2 cut(s) 343, 383
AsuHPI GGTGA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 343, 383
BbvI GCAGC 1 cut(s) 277
BcgI CGANNNNNNTGC 4 cut(s) 350, 384, 449, 483
BciVI GTATCC 1 cut(s) 319
BcoDI GTCTC 2 cut(s) 100, 305
BfaI CTAG 2 cut(s) 42, 50
BfuI GTATCC 1 cut(s) 319
BisI GCNGC 2 cut(s) 291, 391
BlsI GCNGC 2 cut(s) 292, 392
Bme18I GGWCC 2 cut(s) 343, 383
BmgT120I GGNCC 2 cut(s) 343, 383
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 2 cut(s) 457, 592
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 2 cut(s) 117, 190
Bpu10I CCTNAGC 1 cut(s) 423
Bsa29I ATCGAT 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 349
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 322
Bse1I ACTGG 3 cut(s) 134, 316, 380
BseCI ATCGAT 1 cut(s) 459
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 2 cut(s) 321, 322
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 3 cut(s) 134, 316, 380
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 277
BshVI ATCGAT 1 cut(s) 459
BsiSI CCGG 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 321, 322
BsmAI GTCTC 2 cut(s) 100, 305
BsmBI CGTCTC 1 cut(s) 100
Bso31I GGTCTC 1 cut(s) 305
Bsp1407I TGTACA 1 cut(s) 257
Bsp143I GATC 2 cut(s) 456, 460
BspACI CCGC 3 cut(s) 381, 391, 405
BspCNI CTCAG 1 cut(s) 436
BspDI ATCGAT 1 cut(s) 459
BspHI TCATGA 1 cut(s) 463
BspTNI GGTCTC 1 cut(s) 305
BsrGI TGTACA 1 cut(s) 257
BsrI ACTGG 3 cut(s) 134, 316, 380
BssMI GATC 2 cut(s) 456, 460
Bst4CI ACNGT 3 cut(s) 91, 331, 347
Bst6I CTCTTC 3 cut(s) 256, 530, 553
BstAUI TGTACA 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 607
BstKTI GATC 2 cut(s) 459, 463
BstMAI GTCTC 2 cut(s) 100, 305
BstMBI GATC 2 cut(s) 456, 460
BstMWI GCNNNNNNNGC 2 cut(s) 287, 387
BstV1I GCAGC 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 459
BsuI GTATCC 1 cut(s) 319
BsuTUI ATCGAT 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 607
Cac8I GCNNGC 1 cut(s) 513
CciI TCATGA 1 cut(s) 463
Cfr13I GGNCC 2 cut(s) 343, 383
ClaI ATCGAT 1 cut(s) 459
CpoI CGGWCCG 1 cut(s) 343
Csp6I GTAC 1 cut(s) 258
CspI CGGWCCG 1 cut(s) 343
CviAII CATG 2 cut(s) 82, 464
CviJI RGCY 3 cut(s) 210, 263, 586
CviKI_1 RGCY 3 cut(s) 210, 263, 586
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 1 cut(s) 423
DpnI GATC 2 cut(s) 458, 462
DpnII GATC 2 cut(s) 456, 460
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 3 cut(s) 256, 530, 553
EarI CTCTTC 3 cut(s) 256, 530, 553
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 2 cut(s) 343, 383
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 2 cut(s) 85, 467
FaiI YATR 8 cut(s) 83, 410, 432, 465, 504, 597, 620, 631
FalI AAGNNNNNCTT 4 cut(s) 185, 217, 570, 602
FatI CATG 2 cut(s) 81, 463
FauI CCCGC 1 cut(s) 412
Fnu4HI GCNGC 2 cut(s) 291, 391
FokI GGATG 1 cut(s) 614
Fsp4HI GCNGC 2 cut(s) 291, 391
FspBI CTAG 2 cut(s) 42, 50
GluI GCNGC 2 cut(s) 291, 391
GsuI CTGGAG 2 cut(s) 117, 190
HapII CCGG 1 cut(s) 350
Hin1II CATG 2 cut(s) 85, 467
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 2 cut(s) 520, 614
HpaII CCGG 1 cut(s) 350
HphI GGTGA 1 cut(s) 156
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 7 cut(s) 50, 299, 323, 425, 464, 529, 548
Hpy8I GTNNAC 1 cut(s) 14
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 3 cut(s) 91, 331, 347
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 3 cut(s) 153, 470, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 387
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 2 cut(s) 85, 467
Kzo9I GATC 2 cut(s) 456, 460
LmnI GCTCC 1 cut(s) 268
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 2 cut(s) 457, 592
MaeI CTAG 2 cut(s) 42, 50
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 145
MalI GATC 2 cut(s) 458, 462
MboI GATC 2 cut(s) 456, 460
MboII GAAGA 4 cut(s) 243, 500, 517, 570
MfeI CAATTG 1 cut(s) 276
MluCI AATT 5 cut(s) 100, 276, 396, 483, 570
MnlI CCTC 4 cut(s) 29, 34, 141, 556
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 6 cut(s) 336, 395, 453, 482, 582, 588
MspI CCGG 1 cut(s) 350
MunI CAATTG 1 cut(s) 276
MwoI GCNNNNNNNGC 2 cut(s) 287, 387
NdeII GATC 2 cut(s) 456, 460
NlaIII CATG 2 cut(s) 85, 467
PagI TCATGA 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 520, 614
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 292, 392
PshBI ATTAAT 1 cut(s) 482
Psp1406I AACGTT 1 cut(s) 10
PspPI GGNCC 2 cut(s) 343, 383
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
Rsr2I CGGWCCG 1 cut(s) 343
RsrII CGGWCCG 1 cut(s) 343
SaqAI TTAA 6 cut(s) 336, 395, 453, 482, 582, 588
SatI GCNGC 2 cut(s) 291, 391
Sau3AI GATC 2 cut(s) 456, 460
Sau96I GGNCC 2 cut(s) 343, 383
SetI ASST 4 cut(s) 13, 212, 265, 588
SfaNI GCATC 2 cut(s) 457, 592
SinI GGWCC 2 cut(s) 343, 383
Sse9I AATT 5 cut(s) 100, 276, 396, 483, 570
SsiI CCGC 3 cut(s) 381, 391, 405
SspMI CTAG 2 cut(s) 42, 50
TaaI ACNGT 3 cut(s) 91, 331, 347
TaiI ACGT 1 cut(s) 13
TaqI TCGA 2 cut(s) 360, 459
TasI AATT 5 cut(s) 100, 276, 396, 483, 570
TatI WGTACW 1 cut(s) 257
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 2 cut(s) 520, 614
Tru1I TTAA 6 cut(s) 336, 395, 453, 482, 582, 588
Tru9I TTAA 6 cut(s) 336, 395, 453, 482, 582, 588
TseI GCWGC 1 cut(s) 290
TspGWI ACGGA 1 cut(s) 128
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 343, 383
VspI ATTAAT 1 cut(s) 482
XbaI TCTAGA 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 2 cut(s) 42, 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.