Rh4BG085600

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015079
Physical Location & Seq
Forward (+)
14735152 .. 14737078
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG085600.1

Sequence Viewer

Length: 552 bp
ATGGACACCGTCGTCTCCAATTCAGCATTACTATCCATACCACCCTTCCCTCCAGTACGTACAGTTACTTGCAACTCTTATTCACCAACCACTTCTCTCAATCTCTCGTCAAAGAAGCACTTCTCCAGCTTCTTCTACACCCAAAAGAAACAAGTTGGTCATCGCAACTCTTCTTGTACAGCTCCCAAGTTTTCAATTGCTTCAAGTGCTGCTATTCAGGAAATCAACGAGACCCAGTTTCGGGATACGGTTCTTAACTCGGACCGTCCGGTTCTTGTCGAGTTTGTTGCCAACTGGTGCGGTCCTTGCCGCTTAATTTCTCCCGCTATGGAATGGGTTGCTCAGGAATATAAAGACAGATTAGCAGTTGTTAAGATCGATCATGATGCAAACCCACGATTAATTGAAGAATACAAAGTTTATGGTTTGCCTGCTTTGATTCTCTTCAAGAATGGACAGGAAGTTCCAGAAAGTAGAAGAGAGGGTGCAATTACAAAAGTTAAGCTTAAAGAGTATGTGGATGCTTTTTTGGAATCTATATCAGTCGCATAG

Protein Analysis

183

Amino Acids

20.47

Weight (kDa)

8.32

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 73 - 174 1.7e-27 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015079)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50320
fragaria_vesca FvH4_4g06760
malus_domestica MD15G1433100.v1.1
prunus_persica Prupe.1G070700_v2.0.a1
pyrus_communis pycom13g21100 pycom16g20440
rosa_chinensis RchiOBHm_Chr4g0398691
rosa_laevigata RLG00000009331
rosa_multiflora Rmu_sc0008600.1_g000003
rosa_roxburghii Rroxscaffold_5G00343650
rosa_rugosa Rorug04G0010900
rosa_samantha Rh4AG088600 Rh4BG085600 Rh4CG096700 Rh4DG080200
rosa_wichuraiana Rw4G007330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AciI CCGC 3 cut(s) 300, 310, 324
AfaI GTAC 3 cut(s) 57, 61, 178
AfiI CCNNNNNNNGG 2 cut(s) 240, 241
AgsI TTSAA 4 cut(s) 195, 204, 407, 448
AloI GAACNNNNNNTCC 2 cut(s) 447, 479
AluBI AGCT 3 cut(s) 129, 182, 505
AluI AGCT 3 cut(s) 129, 182, 505
Alw26I GTCTC 2 cut(s) 19, 224
ApeKI GCWGC 1 cut(s) 209
AseI ATTAAT 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 262, 302
AsuHPI GGTGA 1 cut(s) 75
AvaII GGWCC 2 cut(s) 262, 302
BbvI GCAGC 1 cut(s) 196
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 368, 402
BciVI GTATCC 1 cut(s) 238
BcoDI GTCTC 2 cut(s) 19, 224
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 2 cut(s) 210, 310
BlsI GCNGC 2 cut(s) 211, 311
Bme18I GGWCC 2 cut(s) 262, 302
BmgT120I GGNCC 2 cut(s) 262, 302
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 2 cut(s) 376, 511
BmuI ACTGGG 1 cut(s) 229
BpmI CTGGAG 2 cut(s) 36, 109
Bpu10I CCTNAGC 1 cut(s) 342
Bsa29I ATCGAT 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 224
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 241
Bse1I ACTGG 3 cut(s) 53, 235, 299
BseCI ATCGAT 1 cut(s) 378
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 2 cut(s) 240, 241
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 3 cut(s) 53, 235, 299
BseXI GCAGC 1 cut(s) 196
BshVI ATCGAT 1 cut(s) 378
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 240, 241
BsmAI GTCTC 2 cut(s) 19, 224
BsmBI CGTCTC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 176
Bsp143I GATC 2 cut(s) 375, 379
BspACI CCGC 3 cut(s) 300, 310, 324
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 378
BspHI TCATGA 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 224
BsrGI TGTACA 1 cut(s) 176
BsrI ACTGG 3 cut(s) 53, 235, 299
BssMI GATC 2 cut(s) 375, 379
Bst4CI ACNGT 4 cut(s) 10, 64, 250, 266
Bst6I CTCTTC 3 cut(s) 175, 449, 472
BstAUI TGTACA 1 cut(s) 176
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 526
BstKTI GATC 2 cut(s) 378, 382
BstMAI GTCTC 2 cut(s) 19, 224
BstMBI GATC 2 cut(s) 375, 379
BstMWI GCNNNNNNNGC 2 cut(s) 206, 306
BstSNI TACGTA 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 196
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 238
BsuTUI ATCGAT 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 1 cut(s) 382
Cfr13I GGNCC 2 cut(s) 262, 302
ClaI ATCGAT 1 cut(s) 378
CpoI CGGWCCG 1 cut(s) 262
Csp6I GTAC 3 cut(s) 56, 60, 177
CspI CGGWCCG 1 cut(s) 262
CviAII CATG 1 cut(s) 383
CviJI RGCY 3 cut(s) 129, 182, 505
CviKI_1 RGCY 3 cut(s) 129, 182, 505
CviQI GTAC 3 cut(s) 56, 60, 177
DdeI CTNAG 1 cut(s) 342
DpnI GATC 2 cut(s) 377, 381
DpnII GATC 2 cut(s) 375, 379
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Eam1104I CTCTTC 3 cut(s) 175, 449, 472
EarI CTCTTC 3 cut(s) 175, 449, 472
Eco105I TACGTA 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 262, 302
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 1 cut(s) 386
FaiI YATR 8 cut(s) 38, 329, 351, 384, 423, 516, 539, 550
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 489, 521
FatI CATG 1 cut(s) 382
FauI CCCGC 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 210, 310
FokI GGATG 1 cut(s) 533
Fsp4HI GCNGC 2 cut(s) 210, 310
GluI GCNGC 2 cut(s) 210, 310
GsuI CTGGAG 2 cut(s) 36, 109
HapII CCGG 1 cut(s) 269
Hin1II CATG 1 cut(s) 386
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 2 cut(s) 439, 533
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 6 cut(s) 218, 242, 344, 383, 448, 467
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 4 cut(s) 10, 64, 250, 266
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 3 cut(s) 72, 389, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 306
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 58
Hsp92II CATG 1 cut(s) 386
Kzo9I GATC 2 cut(s) 375, 379
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 376, 511
MaeII ACGT 1 cut(s) 58
MaeIII GTNAC 1 cut(s) 64
MalI GATC 2 cut(s) 377, 381
MboI GATC 2 cut(s) 375, 379
MboII GAAGA 5 cut(s) 124, 162, 419, 436, 489
MfeI CAATTG 1 cut(s) 195
MluCI AATT 5 cut(s) 19, 195, 315, 402, 489
MnlI CCTC 2 cut(s) 60, 475
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
MspI CCGG 1 cut(s) 269
MunI CAATTG 1 cut(s) 195
MwoI GCNNNNNNNGC 2 cut(s) 206, 306
NdeII GATC 2 cut(s) 375, 379
NlaIII CATG 1 cut(s) 386
PagI TCATGA 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 439, 533
PflFI GACNNNGTC 1 cut(s) 8
PkrI GCNGC 2 cut(s) 211, 311
Ppu21I YACGTR 1 cut(s) 59
PshBI ATTAAT 1 cut(s) 401
PspPI GGNCC 2 cut(s) 262, 302
PsyI GACNNNGTC 1 cut(s) 8
RsaI GTAC 3 cut(s) 57, 61, 178
RsaNI GTAC 3 cut(s) 56, 60, 177
Rsr2I CGGWCCG 1 cut(s) 262
RsrII CGGWCCG 1 cut(s) 262
SaqAI TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
SatI GCNGC 2 cut(s) 210, 310
Sau3AI GATC 2 cut(s) 375, 379
Sau96I GGNCC 2 cut(s) 262, 302
SetI ASST 4 cut(s) 61, 131, 184, 507
SfaNI GCATC 2 cut(s) 376, 511
SinI GGWCC 2 cut(s) 262, 302
SnaBI TACGTA 1 cut(s) 59
Sse9I AATT 5 cut(s) 19, 195, 315, 402, 489
SsiI CCGC 3 cut(s) 300, 310, 324
TaaI ACNGT 4 cut(s) 10, 64, 250, 266
TaiI ACGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 279, 378
TasI AATT 5 cut(s) 19, 195, 315, 402, 489
TatI WGTACW 1 cut(s) 176
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 2 cut(s) 439, 533
Tru1I TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
Tru9I TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
TseI GCWGC 1 cut(s) 209
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 2 cut(s) 262, 302
VspI ATTAAT 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.