RchiOBHm_Chr4g0403441

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015658
Physical Location & Seq
Reverse (-)
22644254 .. 22645741
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37517

Sequence Viewer

Length: 552 bp
ATGTGGTATGCAGGCTATTTCAGTCATGTATGTTGGTCATCTTGGGGAACTATGACTTGCAGGTGCTTCTACGACAACTTCTTTTGCATCAGTCACTGGTTTGAGCTTGATTATGTTCTCCGTTCCAGAGATGATCTAAGATTTGTGGCTTCTTGTGTGTTTGGAATTGGGATGTTCCGGTGCTGTCATTCGAGTTCGTTGTTGGCTCTTTCCAAGCTTTTCGACTCTGGGCCGATGATGTTCTTCAAAATTGGTGCGAATTTTGTTCGCGGTGGCTCTATCGCTATGGCAGCGTCCGTTGCTTGCCTAGATTGGGCTCGATCGCCTGGTTTGAGGCCTTCTTGTTGTTGTCTTTGTCTCTGTGTTTATGTTGTAGTTTGGATAGGAATGGGTAGCGCATTGGACACATTTTGTGGGCAGTCCTATGGGGCAAAACAGTATCATATGCTTGGTGTACACATGCAGAGGGCAATGCTTGTTATTCTTTTGGCCTGCATTCCTCTTGCAACTATATGGTTCAATGCAGGCCACATTCTCAAATTCTTGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

183

Amino Acids

20.54

Weight (kDa)

8.51

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 123 - 183 8.1e-13 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 51
Acc36I ACCTGC 1 cut(s) 51
AccII CGCG 1 cut(s) 270
AciI CCGC 1 cut(s) 270
AcsI RAATTY 2 cut(s) 259, 539
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 2 cut(s) 247, 520
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 2 cut(s) 106, 217
AluI AGCT 2 cut(s) 106, 217
Alw26I GTCTC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 96
AoxI GGCC 4 cut(s) 230, 335, 489, 526
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 2 cut(s) 259, 539
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 362
BfaI CTAG 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 51
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 230
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 327
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMI GCAATG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 101
BseXI GCAGC 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 323
BshFI GGCC 4 cut(s) 232, 337, 491, 528
BsiEI CGRYCG 1 cut(s) 323
BsiSI CCGG 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 1 cut(s) 362
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 4 cut(s) 232, 337, 491, 528
Bsp1286I GDGCHC 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 2 cut(s) 133, 320
BspACI CCGC 1 cut(s) 270
BspANI GGCC 4 cut(s) 232, 337, 491, 528
BspFNI CGCG 1 cut(s) 270
BspMI ACCTGC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 477
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 101
BssMI GATC 2 cut(s) 133, 320
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 438
BstAUI TGTACA 1 cut(s) 454
BstC8I GCNNGC 4 cut(s) 13, 304, 493, 526
BstDEI CTNAG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 2 cut(s) 136, 323
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 133, 320
BstMCI CGRYCG 1 cut(s) 323
BstMWI GCNNNNNNNGC 2 cut(s) 290, 299
BstNI CCWGG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 325
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 302
BsuRI GGCC 4 cut(s) 232, 337, 491, 528
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 51
Cac8I GCNNGC 4 cut(s) 13, 304, 493, 526
CaiI CAGNNNCTG 1 cut(s) 96
CfoI GCGC 1 cut(s) 398
Cfr13I GGNCC 1 cut(s) 230
CseI GACGC 1 cut(s) 282
Csp6I GTAC 1 cut(s) 455
CviAII CATG 2 cut(s) 26, 460
CviQI GTAC 1 cut(s) 455
DdeI CTNAG 1 cut(s) 137
DpnI GATC 2 cut(s) 135, 322
DpnII GATC 2 cut(s) 133, 320
Eco147I AGGCCT 1 cut(s) 337
Eco24I GRGCYC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 319
FaeI CATG 2 cut(s) 29, 463
FatI CATG 2 cut(s) 25, 459
FauNDI CATATG 1 cut(s) 444
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 1 cut(s) 184
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 308
GlaI GCGC 1 cut(s) 397
GluI GCNGC 1 cut(s) 291
HaeIII GGCC 4 cut(s) 232, 337, 491, 528
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 282
HhaI GCGC 1 cut(s) 398
Hin1II CATG 2 cut(s) 29, 463
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 455, 457
Hpy188III TCNNGA 1 cut(s) 126
Hpy8I GTNNAC 2 cut(s) 455, 457
HpyAV CCTTC 1 cut(s) 348
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4V TGCA 7 cut(s) 11, 60, 87, 463, 495, 506, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 299
HpyF3I CTNAG 1 cut(s) 137
Hsp92II CATG 2 cut(s) 29, 463
HspAI GCGC 1 cut(s) 396
Kzo9I GATC 2 cut(s) 133, 320
LpnPI CCDG 9 cut(s) 46, 82, 139, 191, 213, 312, 339, 505, 510
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 92
MalI GATC 2 cut(s) 135, 322
MboI GATC 2 cut(s) 133, 320
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 4 cut(s) 165, 249, 259, 539
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 3 cut(s) 327, 459, 510
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 1 cut(s) 327
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 290, 299
NdeI CATATG 1 cut(s) 444
NdeII GATC 2 cut(s) 133, 320
NlaIII CATG 2 cut(s) 29, 463
NmuCI GTSAC 1 cut(s) 92
NspI RCATGY 1 cut(s) 463
PaqCI CACCTGC 1 cut(s) 51
PceI AGGCCT 1 cut(s) 337
PctI GAATGC 1 cut(s) 495
PkrI GCNGC 1 cut(s) 292
Ple19I CGATCG 1 cut(s) 323
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspPI GGNCC 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 96
PvuI CGATCG 1 cut(s) 323
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 2 cut(s) 133, 320
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 319
SetI ASST 3 cut(s) 65, 108, 219
SfaNI GCATC 1 cut(s) 96
Sse9I AATT 4 cut(s) 165, 249, 259, 539
SseBI AGGCCT 1 cut(s) 337
SsiI CCGC 1 cut(s) 270
SspMI CTAG 1 cut(s) 308
StuI AGGCCT 1 cut(s) 337
StyD4I CCNGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 438
TaqI TCGA 3 cut(s) 191, 222, 319
TasI AATT 4 cut(s) 165, 249, 259, 539
TatI WGTACW 1 cut(s) 454
TscAI CASTG 1 cut(s) 101
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 92
TspGWI ACGGA 2 cut(s) 110, 286
TspRI CASTG 1 cut(s) 101
XapI RAATTY 2 cut(s) 259, 539
XceI RCATGY 1 cut(s) 463
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.