RchiOBHm_Chr5g0004011

pyruvate, phosphate dikinase regulatory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013019
Physical Location & Seq
Forward (+)
2524175 .. 2526208
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28529

Sequence Viewer

Length: 1236 bp
ATGTCCCGTTACAACAACAAAACCAAAACCCAGGCAAAAAAATTTAACAATAAAATAATGATAGCTTCTTCCACCTTCACTCTCCGGAACCTCCGCGTCTCACTCGCCGACCCCCACCGTATACCCAAACCTCCACAGACCTCATACGAACCTGGATCCGAGCCTCAGCCTCGGAAGTTCAAGGCCAGCCCTCAGCTAAGCCGATGGTCCCGGGCCCGGTCCTTGCGCTCCGGTCGGAAATTCGACCGGACCGGCCAGCAGACCCAACTACTCGACCCAAACCCTCCTCCGCAGCTCAAAGAACGAGTCTTGCCGGAGCAAAAGGACACTTCTTTAACGGCGAGTGACGGCGAAGATGACGTGGTCATGACGGCCGGGAAGTCGATTTATATGGTTTCCGACGGAACTGGGTGGACGGTGGAACACTCCGTCGGCGCAGCGTTGGGCCAGTTTGAGCATTGCTTGGTCGATCGGAATTGCGCTGTGAATACCCATTTGTTTTCTGGGATTGATGATGTGGGGCAGTTAATGGAGATTATAAAGCAAGCAGCTAAAGAAGGAGCTATGGTGGTGTACACATTAGCTGACCCATCAATGGCTGAGTCTGCTAAACAAGCTTGTAAAATTTGGGGCTTACCCAGCATTGATATGTTAGGTCCAATTACACAGTCAATAGCTTCACATCTAAATGTGTCACCTTCTGGGGGTCCTAGGAGATACCCTCTTACAGATGAGTACTTTCAAAGGATTGAAGCGATTGAGTTTACGATCAAGCAAGATGATGGGGCATCGCCCCAGAACTTGCACAAGGCCGACATTGTGCTTGCCGGAGTTTCGAGAACGGGGAAGACCCCGTTGTCGATTTATCTGTCACAGAAGGGGTACAAGGTGGCTAATGTGCCTATTGTGATGGGGGTTCCTTTGCCAAAGACTCTTTTTGAGATTGACCCCGAGAAAGTTTTTGGCTTGACTATTAATCCTCTGGTGTTGCAAACGATTAGAAGGGCGAGGGCTAAGAGTTTGGGGTTCAGTGAAGGAGTGAGGAGTAACTACTCTGAGATGGATCATGTTCGAGAGGAGCTTGAGTATGCACGAAAGATTCTTGCACAAAATCCTGTCTGGCCAGTAATTGAAGTGACAGGAAAAGCAATAGAAGAAACTGCAGCTGTTGTATTGAGGCTTTATCATGACCGGAAGAACAAGTGCTCAATGCCAAGAATATCAAAACGTTATTGA

Protein Analysis

411

Amino Acids

45.76

Weight (kDa)

9.45

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinase-PPPase PF03618 129 - 392 4.6e-88 Kinase/pyrophosphorylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21210 AT4G21210
fragaria_vesca FvH4_3g02680
malus_domestica MD05G1340900.v1.1
prunus_persica Prupe.4G026400_v2.0.a1
pyrus_communis pycom05g31020 pycom10g26650
rosa_chinensis RchiOBHm_Chr5g0004011
rosa_laevigata RLG00000031196
rosa_multiflora Rmu_co8460589.1_g000001 Rmu_sc0000547.1_g000016
rosa_roxburghii Rroxscaffold_1G00071960
rosa_rugosa Rorug04G0409000
rosa_samantha Rh5AG036700 Rh5BG035800 Rh5CG039400 Rh5DG035200
rosa_wichuraiana Rw5G003330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AasI GACNNNNNNGTC 1 cut(s) 856
AccI GTMKAC 1 cut(s) 121
AccII CGCG 1 cut(s) 96
AccIII TCCGGA 1 cut(s) 84
AciI CCGC 2 cut(s) 94, 290
AclI AACGTT 1 cut(s) 1228
AclWI GGATC 3 cut(s) 150, 163, 1071
AcoI YGGCCR 3 cut(s) 253, 372, 1121
AcsI RAATTY 3 cut(s) 41, 239, 624
AfaI GTAC 3 cut(s) 575, 737, 884
AfiI CCNNNNNNNGG 3 cut(s) 216, 595, 704
AgsI TTSAA 4 cut(s) 181, 743, 752, 1133
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 2 cut(s) 30, 151
Alw21I GWGCWC 1 cut(s) 1208
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 3 cut(s) 150, 163, 1071
Ama87I CYCGRG 2 cut(s) 210, 950
Aor13HI TCCGGA 1 cut(s) 84
AoxI GGCC 7 cut(s) 183, 213, 253, 372, 445, 810, 1121
ApaI GGGCCC 1 cut(s) 217
ApeKI GCWGC 4 cut(s) 292, 437, 548, 1163
ApoI RAATTY 3 cut(s) 41, 239, 624
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AseI ATTAAT 1 cut(s) 975
AspA2I CCTAGG 1 cut(s) 710
AspLEI GCGC 3 cut(s) 228, 437, 482
AspS9I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
AsuC2I CCSGG 4 cut(s) 211, 212, 217, 376
AsuHPI GGTGA 1 cut(s) 687
AvaI CYCGRG 2 cut(s) 210, 950
AvaII GGWCC 5 cut(s) 207, 219, 249, 656, 707
AvrII CCTAGG 1 cut(s) 710
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 1123
BamHI GGATCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 854
Bbv12I GWGCWC 1 cut(s) 1208
BbvCI CCTCAGC 2 cut(s) 165, 192
BbvI GCAGC 4 cut(s) 304, 449, 560, 1175
BccI CCATC 5 cut(s) 198, 598, 776, 904, 1054
BceAI ACGGC 3 cut(s) 354, 364, 387
BciT130I CCWGG 2 cut(s) 32, 153
BcnI CCSGG 4 cut(s) 211, 212, 217, 376
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 1 cut(s) 1161
BisI GCNGC 4 cut(s) 293, 438, 549, 1164
BlnI CCTAGG 1 cut(s) 710
BlpI GCTNAGC 1 cut(s) 197
BlsI GCNGC 4 cut(s) 294, 439, 550, 1165
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
Bme18I GGWCC 5 cut(s) 207, 219, 249, 656, 707
BmeT110I CYCGRG 2 cut(s) 210, 950
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
BmiI GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
BmrFI CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 1 cut(s) 797
BmuI ACTGGG 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 854
BplI GAGNNNNNCTC 2 cut(s) 706, 738
Bpu10I CCTNAGC 2 cut(s) 165, 192
Bpu1102I GCTNAGC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 4 cut(s) 211, 212, 217, 376
BsaJI CCNNGG 4 cut(s) 30, 170, 210, 710
BsaWI WCCGGW 4 cut(s) 84, 230, 246, 1191
BsaXI ACNNNNNCTCC 4 cut(s) 552, 582, 1070, 1100
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 595, 704
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 3 cut(s) 412, 448, 1124
Bse3DI GCAATG 1 cut(s) 457
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 2 cut(s) 32, 153
BseDI CCNNGG 4 cut(s) 30, 170, 210, 710
BseLI CCNNNNNNNGG 3 cut(s) 216, 595, 704
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 4 cut(s) 179, 206, 591, 1047
BseNI ACTGG 3 cut(s) 412, 448, 1124
BseRI GAGGAG 3 cut(s) 276, 1057, 1091
BseSI GKGCMC 1 cut(s) 217
BseX3I CGGCCG 1 cut(s) 372
BseXI GCAGC 4 cut(s) 304, 449, 560, 1175
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 4 cut(s) 235, 247, 375, 472
BshFI GGCC 7 cut(s) 185, 215, 255, 374, 447, 812, 1123
BsiEI CGRYCG 4 cut(s) 235, 247, 375, 472
BsiHKAI GWGCWC 1 cut(s) 1208
BsiHKCI CYCGRG 2 cut(s) 210, 950
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 3 cut(s) 216, 595, 704
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 7 cut(s) 185, 215, 255, 374, 447, 812, 1123
BsoBI CYCGRG 2 cut(s) 210, 950
Bsp120I GGGCCC 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 217, 1208
Bsp13I TCCGGA 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 4 cut(s) 155, 469, 768, 1063
Bsp1720I GCTNAGC 1 cut(s) 197
BspACI CCGC 2 cut(s) 94, 290
BspANI GGCC 7 cut(s) 185, 215, 255, 374, 447, 812, 1123
BspCNI CTCAG 4 cut(s) 178, 205, 592, 1048
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 2 cut(s) 366, 1186
BspLI GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
BspMAI CTGCAG 1 cut(s) 1165
BspPI GGATC 3 cut(s) 150, 163, 1071
BsrDI GCAATG 1 cut(s) 457
BsrFI RCCGGY 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 3 cut(s) 412, 448, 1124
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 4 cut(s) 30, 170, 210, 710
BssMI GATC 4 cut(s) 155, 469, 768, 1063
BssNAI GTATAC 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 710
Bst1107I GTATAC 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 32, 153
Bst4CI ACNGT 3 cut(s) 119, 418, 669
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 4 cut(s) 187, 257, 546, 825
BstDEI CTNAG 6 cut(s) 165, 192, 197, 600, 1014, 1056
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 3 cut(s) 228, 437, 482
BstKTI GATC 4 cut(s) 158, 472, 771, 1066
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 4 cut(s) 155, 469, 768, 1063
BstMCI CGRYCG 4 cut(s) 235, 247, 375, 472
BstMWI GCNNNNNNNGC 3 cut(s) 605, 614, 639
BstNI CCWGG 2 cut(s) 32, 153
BstSCI CCNGG 6 cut(s) 30, 151, 209, 210, 215, 374
BstSFI CTRYAG 1 cut(s) 1161
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 304, 449, 560, 1175
BstV2I GAAGAC 1 cut(s) 854
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BstZ17I GTATAC 1 cut(s) 122
BstZI CGGCCG 1 cut(s) 372
BsuRI GGCC 7 cut(s) 185, 215, 255, 374, 447, 812, 1123
BtgZI GCGATG 1 cut(s) 774
BtrI CACGTC 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 1036
Cac8I GCNNGC 4 cut(s) 187, 257, 546, 825
CciI TCATGA 2 cut(s) 366, 1186
CfoI GCGC 3 cut(s) 228, 437, 482
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
Cfr9I CCCGGG 1 cut(s) 210
CpoI CGGWCCG 1 cut(s) 249
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 574, 736, 883
CspI CGGWCCG 1 cut(s) 249
CviAII CATG 3 cut(s) 367, 1067, 1187
CviQI GTAC 3 cut(s) 574, 736, 883
DdeI CTNAG 6 cut(s) 165, 192, 197, 600, 1014, 1056
DpnI GATC 4 cut(s) 157, 471, 770, 1065
DpnII GATC 4 cut(s) 155, 469, 768, 1063
DrdI GACNNNNNNGTC 1 cut(s) 856
DseDI GACNNNNNNGTC 1 cut(s) 856
EaeI YGGCCR 3 cut(s) 253, 372, 1121
EagI CGGCCG 1 cut(s) 372
EclXI CGGCCG 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 710
Eco24I GRGCYC 1 cut(s) 217
Eco47I GGWCC 5 cut(s) 207, 219, 249, 656, 707
Eco52I CGGCCG 1 cut(s) 372
Eco88I CYCGRG 2 cut(s) 210, 950
EcoO109I RGGNCCY 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 30, 151
EcoT14I CCWWGG 1 cut(s) 710
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 710
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 3 cut(s) 370, 1070, 1190
FaqI GGGAC 1 cut(s) 193
FatI CATG 3 cut(s) 366, 1066, 1186
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 293, 438, 549, 1164
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 4 cut(s) 293, 438, 549, 1164
FspBI CTAG 1 cut(s) 711
GlaI GCGC 3 cut(s) 227, 436, 481
GluI GCNGC 4 cut(s) 293, 438, 549, 1164
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 7 cut(s) 185, 215, 255, 374, 447, 812, 1123
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 228, 437, 482
Hin1II CATG 3 cut(s) 370, 1070, 1190
Hin6I GCGC 3 cut(s) 226, 435, 480
HinP1I GCGC 3 cut(s) 226, 435, 480
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 4 cut(s) 306, 602, 931, 1099
HphI GGTGA 1 cut(s) 687
Hpy166II GTNNAC 5 cut(s) 122, 414, 574, 576, 765
Hpy188I TCNGA 6 cut(s) 160, 174, 237, 400, 474, 1057
Hpy188III TCNNGA 5 cut(s) 85, 367, 837, 1073, 1187
Hpy8I GTNNAC 5 cut(s) 122, 414, 574, 576, 765
Hpy99I CGWCG 2 cut(s) 404, 434
HpyAV CCTTC 6 cut(s) 85, 551, 708, 871, 996, 1028
HpyCH4III ACNGT 3 cut(s) 119, 418, 669
HpyCH4IV ACGT 2 cut(s) 360, 1228
HpyCH4V TGCA 5 cut(s) 805, 991, 1091, 1106, 1163
HpyF10VI GCNNNNNNNGC 3 cut(s) 605, 614, 639
HpyF3I CTNAG 6 cut(s) 165, 192, 197, 600, 1014, 1056
HpySE526I ACGT 2 cut(s) 360, 1228
Hsp92II CATG 3 cut(s) 370, 1070, 1190
HspAI GCGC 3 cut(s) 226, 435, 480
Kpn2I TCCGGA 1 cut(s) 84
Kzo9I GATC 4 cut(s) 155, 469, 768, 1063
LmnI GCTCC 4 cut(s) 233, 316, 560, 1078
Lsp1109I GCAGC 4 cut(s) 304, 449, 560, 1175
LweI GCATC 1 cut(s) 797
MaeI CTAG 1 cut(s) 711
MaeII ACGT 2 cut(s) 360, 1228
MaeIII GTNAC 6 cut(s) 8, 344, 693, 870, 1046, 1135
MalI GATC 4 cut(s) 157, 471, 770, 1065
MboI GATC 4 cut(s) 155, 469, 768, 1063
MboII GAAGA 5 cut(s) 60, 365, 859, 1166, 1207
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 2 cut(s) 217, 1208
MlsI TGGCCA 1 cut(s) 1123
MluCI AATT 6 cut(s) 41, 239, 475, 624, 660, 1128
MluNI TGGCCA 1 cut(s) 1123
MlyI GAGTC 3 cut(s) 315, 611, 925
MmeI TCCRAC 2 cut(s) 215, 423
Mox20I TGGCCA 1 cut(s) 1123
MroI TCCGGA 1 cut(s) 84
MscI TGGCCA 1 cut(s) 1123
MseI TTAA 4 cut(s) 45, 335, 527, 975
MslI CAYNNNNRTG 2 cut(s) 647, 687
Msp20I TGGCCA 1 cut(s) 1123
MspA1I CMGCKG 1 cut(s) 1166
MspR9I CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
MvaI CCWGG 2 cut(s) 32, 153
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 3 cut(s) 605, 614, 639
NciI CCSGG 4 cut(s) 211, 212, 217, 376
NdeII GATC 4 cut(s) 155, 469, 768, 1063
NlaIII CATG 3 cut(s) 370, 1070, 1190
NlaIV GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
NmuCI GTSAC 4 cut(s) 344, 693, 870, 1135
PagI TCATGA 2 cut(s) 366, 1186
PfeI GAWTC 1 cut(s) 1099
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 4 cut(s) 294, 439, 550, 1165
Ple19I CGATCG 1 cut(s) 472
PleI GAGTC 3 cut(s) 314, 610, 925
PpsI GAGTC 3 cut(s) 314, 610, 925
PpuMI RGGWCCY 1 cut(s) 707
PshBI ATTAAT 1 cut(s) 975
PsiI TTATAA 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 1228
Psp5II RGGWCCY 1 cut(s) 707
Psp6I CCWGG 2 cut(s) 30, 151
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 2 cut(s) 30, 151
PspN4I GGNNCC 6 cut(s) 89, 157, 209, 215, 708, 918
PspOMI GGGCCC 1 cut(s) 213
PspPI GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
PspPPI RGGWCCY 1 cut(s) 707
PstI CTGCAG 1 cut(s) 1165
PsuI RGATCY 1 cut(s) 155
PsyI GACNNNGTC 1 cut(s) 362
PvuI CGATCG 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 1166
RsaI GTAC 3 cut(s) 575, 737, 884
RsaNI GTAC 3 cut(s) 574, 736, 883
RseI CAYNNNNRTG 2 cut(s) 647, 687
Rsr2I CGGWCCG 1 cut(s) 249
RsrII CGGWCCG 1 cut(s) 249
SaqAI TTAA 4 cut(s) 45, 335, 527, 975
SatI GCNGC 4 cut(s) 293, 438, 549, 1164
Sau3AI GATC 4 cut(s) 155, 469, 768, 1063
Sau96I GGNCC 8 cut(s) 207, 213, 214, 219, 249, 445, 656, 707
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 3 cut(s) 315, 611, 925
ScrFI CCNGG 6 cut(s) 32, 153, 211, 212, 217, 376
SduI GDGCHC 2 cut(s) 217, 1208
SfaNI GCATC 1 cut(s) 797
SfcI CTRYAG 1 cut(s) 1161
SinI GGWCC 5 cut(s) 207, 219, 249, 656, 707
SmaI CCCGGG 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 647, 687
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
Sse9I AATT 6 cut(s) 41, 239, 475, 624, 660, 1128
SsiI CCGC 2 cut(s) 94, 290
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 6 cut(s) 30, 151, 209, 210, 215, 374
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 3 cut(s) 119, 418, 669
TaiI ACGT 2 cut(s) 363, 1231
TaqI TCGA 7 cut(s) 243, 273, 383, 468, 836, 860, 1072
TasI AATT 6 cut(s) 41, 239, 475, 624, 660, 1128
TatI WGTACW 2 cut(s) 573, 735
TfiI GAWTC 1 cut(s) 1099
Tru1I TTAA 4 cut(s) 45, 335, 527, 975
Tru9I TTAA 4 cut(s) 45, 335, 527, 975
TscAI CASTG 1 cut(s) 1036
TseFI GTSAC 4 cut(s) 344, 693, 870, 1135
TseI GCWGC 4 cut(s) 292, 437, 548, 1163
Tsp45I GTSAC 4 cut(s) 344, 693, 870, 1135
TspGWI ACGGA 2 cut(s) 417, 418
TspMI CCCGGG 1 cut(s) 210
TspRI CASTG 1 cut(s) 1036
Tth111I GACNNNGTC 1 cut(s) 362
VpaK11BI GGWCC 5 cut(s) 207, 219, 249, 656, 707
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 3 cut(s) 41, 239, 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmaI CCCGGG 1 cut(s) 210
XmaJI CCTAGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 121
XspI CTAG 1 cut(s) 711
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.